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The map label for this gene is dnaG [H]

Identifier: 119773961

GI number: 119773961

Start: 1008819

End: 1010549

Strand: Reverse

Name: dnaG [H]

Synonym: Sama_0824

Alternate gene names: 119773961

Gene position: 1010549-1008819 (Counterclockwise)

Preceding gene: 119773962

Following gene: 119773960

Centisome position: 23.47

GC content: 52.86

Gene sequence:

>1731_bases
GTGGCAATACCTCGTGATTTCATCAATGATCTGATAGCCAGAACGGACATAGTCGAGCTTATCGACCGTAAAGTGCCGCT
CAAAAAAGCAGGTAAGAACTATTCTGCCTGTTGTCCCTTCCACAGCGAAAAATCACCTTCATTTACCGTCAGCAGAGACA
AGCAGTTTTATCACTGTTTCGGTTGTGGCGCCCATGGTAATGCCATCGACTTCGTGATGGAGTACGACCGGCTTAACTTT
GTCGACGCCATTGAAGACCTGGCAGGACAGCTGGGGTTGGAAGTCCCGCGGGAGCAAGGCACTGGTCCTCGCCGCGACGA
GGGACTCGCCCGGGATCTGTATCAGCTGATGGAAGAGGCCGCGAGGCATTATCAGACACAACTGCGTCAGCACGCCGACA
AACAAAAAGTCGACGAGTATCTGGCACTGCGTGGGCTGAGCAAAGAAGTCGTTGAAGACTTTGGTATCGGTTTTGCCCCC
GATGGCTGGGATGGTCTGTTGGGTCGCTATCGTACCAACCCGGATGCCCAGGACAAGCTGCTGACCGGCGGCATGTTGAT
TGAAAACGATGGTGGCAAGCGATACGACCGCTTCCGCGATCGTTTGATGTTTCCTATTCGCGACAGGCGCGGTCGGGTCA
TAGGTTTCGGTGGCCGGGTGCTGGGTGATGGCACACCAAAATATTTGAATTCGCCGGAAACGCCCATATTTCATAAGGGA
CAGGAACTCTACGGTCTCTATGAGCTGAAGCAAAAGCATCGCGATCCAGAGCGGGTCATGATTGTGGAAGGCTACATGGA
CGTGGTGGCGTTGGCACAATTTGGCATCGACTACGCGGTGGCCTCTCTGGGCACCTCGACAACCGCAGAGCAATTTCAGT
TGCTGCTGCGCAGTGCCAAAGAAGTCATCTGTTGTTATGACGGTGACAAAGCCGGTTATGAGGCTGCCTGGCGGGCAATG
GAAACCGCCCTGCCGCTGCTCAAACCCGGCGACAAAGTGACATTTTTGTTCCTCCCTCAGGGAGAAGATCCGGACTCGCA
AGTCCGCAAAGAAGGCAAAGAAGCCTTTGAAATGCGCATCGATAATGCGCTGCCACTGGCCGATTATTTGTTCGACACCT
TGTCGCAGAAATACGGCACAGATAAAGGCAACCTGGCTAAGCAGGCCATTGCTCTGATTGAAAAAATTAAAGACACGGTT
CTTGCCAATCTGCTGCTGGAAAACCTTGCCCATAAACTTGGGATGAACTCCAGTGAAGATCTCAGGAAAAAACTCGGTCT
AACCAGTATGTCCACTGGTGCCAAGGCGATGGCGAAAAAAGGACTCAAAGGACGGGGTACACCGCTGCGACTGGCCATTT
CACTGCTGGTGCAACACCCCGAACTGGGACACAAACTGCCGGTTCAGCCGGCATTGAGCCACATTCGCATGACGGGTATT
GAGCTGCTCATCCAACTGTTGGAGCTCACCCGGGAACAGGTGCTCTCCACAGCCCAACTGCTCGAGTCCTATCGTGACAG
CGAGCATTGGGGGCCGTTAACTAAACTGGCCCAGTGGGAACACCAGATTGCCGAAGATAATGTGGCCCAGGAATTTCGCA
AAGCGCTGGTATGGCTTAATAATCAATACATAGAGCAGCGCTACCAGGAGCTCAGCATTAAACCCGACTTAACCCGGGAA
GAAAAGCTGCAACTCCAAAAGCTTATCGTTGTGATGAAGGGCATGAAGTAG

Upstream 100 bases:

>100_bases
CCGCGCCAAACTGCAATAATCGACTTTTGTGTATGAACAAGCCGTGCAATTGCGCGGCTTGTTTGTTTAACTGAGCTTTT
AGGAAATTGAAGTAAGCAAC

Downstream 100 bases:

>100_bases
GGCAAAAAACCGCCCTTTCCCTCTGGCACAGCCATAGGCAGATGGCTATAATTGACGATTTGCGCGACTTTGTGGTCCGG
CCACGGGTGCGCTCGTTGTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MAIPRDFINDLIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLNF
VDAIEDLAGQLGLEVPREQGTGPRRDEGLARDLYQLMEEAARHYQTQLRQHADKQKVDEYLALRGLSKEVVEDFGIGFAP
DGWDGLLGRYRTNPDAQDKLLTGGMLIENDGGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
QELYGLYELKQKHRDPERVMIVEGYMDVVALAQFGIDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDKAGYEAAWRAM
ETALPLLKPGDKVTFLFLPQGEDPDSQVRKEGKEAFEMRIDNALPLADYLFDTLSQKYGTDKGNLAKQAIALIEKIKDTV
LANLLLENLAHKLGMNSSEDLRKKLGLTSMSTGAKAMAKKGLKGRGTPLRLAISLLVQHPELGHKLPVQPALSHIRMTGI
ELLIQLLELTREQVLSTAQLLESYRDSEHWGPLTKLAQWEHQIAEDNVAQEFRKALVWLNNQYIEQRYQELSIKPDLTRE
EKLQLQKLIVVMKGMK

Sequences:

>Translated_576_residues
MAIPRDFINDLIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLNF
VDAIEDLAGQLGLEVPREQGTGPRRDEGLARDLYQLMEEAARHYQTQLRQHADKQKVDEYLALRGLSKEVVEDFGIGFAP
DGWDGLLGRYRTNPDAQDKLLTGGMLIENDGGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
QELYGLYELKQKHRDPERVMIVEGYMDVVALAQFGIDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDKAGYEAAWRAM
ETALPLLKPGDKVTFLFLPQGEDPDSQVRKEGKEAFEMRIDNALPLADYLFDTLSQKYGTDKGNLAKQAIALIEKIKDTV
LANLLLENLAHKLGMNSSEDLRKKLGLTSMSTGAKAMAKKGLKGRGTPLRLAISLLVQHPELGHKLPVQPALSHIRMTGI
ELLIQLLELTREQVLSTAQLLESYRDSEHWGPLTKLAQWEHQIAEDNVAQEFRKALVWLNNQYIEQRYQELSIKPDLTRE
EKLQLQKLIVVMKGMK
>Mature_575_residues
AIPRDFINDLIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLNFV
DAIEDLAGQLGLEVPREQGTGPRRDEGLARDLYQLMEEAARHYQTQLRQHADKQKVDEYLALRGLSKEVVEDFGIGFAPD
GWDGLLGRYRTNPDAQDKLLTGGMLIENDGGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKGQ
ELYGLYELKQKHRDPERVMIVEGYMDVVALAQFGIDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDKAGYEAAWRAME
TALPLLKPGDKVTFLFLPQGEDPDSQVRKEGKEAFEMRIDNALPLADYLFDTLSQKYGTDKGNLAKQAIALIEKIKDTVL
ANLLLENLAHKLGMNSSEDLRKKLGLTSMSTGAKAMAKKGLKGRGTPLRLAISLLVQHPELGHKLPVQPALSHIRMTGIE
LLIQLLELTREQVLSTAQLLESYRDSEHWGPLTKLAQWEHQIAEDNVAQEFRKALVWLNNQYIEQRYQELSIKPDLTREE
KLQLQKLIVVMKGMK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=576, Percent_Identity=51.0416666666667, Blast_Score=566, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65128; Mature: 64996

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPRDFINDLIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCF
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEEECCCCEEEEE
GCGAHGNAIDFVMEYDRLNFVDAIEDLAGQLGLEVPREQGTGPRRDEGLARDLYQLMEEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHH
ARHYQTQLRQHADKQKVDEYLALRGLSKEVVEDFGIGFAPDGWDGLLGRYRTNPDAQDKL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHHHH
LTGGMLIENDGGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
EECCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCCHHCCCCCCCCEECC
QELYGLYELKQKHRDPERVMIVEGYMDVVALAQFGIDYAVASLGTSTTAEQFQLLLRSAK
HHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVICCYDGDKAGYEAAWRAMETALPLLKPGDKVTFLFLPQGEDPDSQVRKEGKEAFEMRI
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
DNALPLADYLFDTLSQKYGTDKGNLAKQAIALIEKIKDTVLANLLLENLAHKLGMNSSED
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRKKLGLTSMSTGAKAMAKKGLKGRGTPLRLAISLLVQHPELGHKLPVQPALSHIRMTGI
HHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ELLIQLLELTREQVLSTAQLLESYRDSEHWGPLTKLAQWEHQIAEDNVAQEFRKALVWLN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQYIEQRYQELSIKPDLTREEKLQLQKLIVVMKGMK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIPRDFINDLIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCF
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEEECCCCEEEEE
GCGAHGNAIDFVMEYDRLNFVDAIEDLAGQLGLEVPREQGTGPRRDEGLARDLYQLMEEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHH
ARHYQTQLRQHADKQKVDEYLALRGLSKEVVEDFGIGFAPDGWDGLLGRYRTNPDAQDKL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHHHH
LTGGMLIENDGGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
EECCEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCCHHCCCCCCCCEECC
QELYGLYELKQKHRDPERVMIVEGYMDVVALAQFGIDYAVASLGTSTTAEQFQLLLRSAK
HHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EVICCYDGDKAGYEAAWRAMETALPLLKPGDKVTFLFLPQGEDPDSQVRKEGKEAFEMRI
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
DNALPLADYLFDTLSQKYGTDKGNLAKQAIALIEKIKDTVLANLLLENLAHKLGMNSSED
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRKKLGLTSMSTGAKAMAKKGLKGRGTPLRLAISLLVQHPELGHKLPVQPALSHIRMTGI
HHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ELLIQLLELTREQVLSTAQLLESYRDSEHWGPLTKLAQWEHQIAEDNVAQEFRKALVWLN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQYIEQRYQELSIKPDLTREEKLQLQKLIVVMKGMK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]