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The map label for this gene is rpoD [H]

Identifier: 119773960

GI number: 119773960

Start: 1006839

End: 1008701

Strand: Reverse

Name: rpoD [H]

Synonym: Sama_0823

Alternate gene names: 119773960

Gene position: 1008701-1006839 (Counterclockwise)

Preceding gene: 119773961

Following gene: 119773957

Centisome position: 23.42

GC content: 52.93

Gene sequence:

>1863_bases
ATGATATCTATGGATCATACTCCGCAGTCGCAACTAAAGATGTTGCTCGCCAAAGGTAAAGAGCAAGGTTACTTAACCTA
TGCAGAAGTGAACGATCACTTGCCTGCAGATATGGTCGACTCTGACCAGATCGAAGATATTATCCAGATGATCAATGACA
TGGGTATCCGCGTTTATGAAGAAGCGCCCGATGCCGATGAAATGATGATGTCCGAAGACAACACGGACGAAGATGCCGCC
GAAGAAGCAGCGGCTGCGCTGGCCACAGTAGAAAGCGAACTTGGCCGCACCACTGACCCGGTACGCATGTACATGCGTGA
AATGGGTACTGTTGAGCTGCTCACCCGTGAAGGCGAAATCGTTATCGCCAAGCGTATCGAAGAAGGCATCAACACTGTTC
AAAGCTCTGTGGCTGAATATCCACAAGCTATCTCCATGATCCTGGAGCAATTCGACCAGTTCGAAGCAGACGCCATTCGT
CTGTCTGATATCATTTCCGGCTTTGTGGACCCCAACGAAGAAGACGTTGGCCCCACCGCGACCCATATTGGTTCCGAGCT
TTCAGAAGAAGAGCTCGAGGACGAAGACGATATGGACGAAGACGAAGAGGAAGACGAAGACGGCGACAGCAGCGATGACG
ACGGCGCCAAGGGCCCGGATCCGGAAGTCGCCCGTGAACGCTTTGCCGCACTGCGCGCCGCCTATGACAATGCACTGAAA
GTCATTGAAGTCAAAGGTCGTGCTCATCCCGAGTCTACCCGCGCCCTGTTTGAACTCGGTGAAATCTTCAAAGAATTCCG
TCTGGTGCCCAAGCAGTTTGACCGTCTGGTCAAGAGCATGCGCGAGATGATGGACAAGGTTCGTGTTCAGGAACGTCTGA
TCATGAAACTGTGCGTTGAACAGGCCAAGATGCCGAAGAAAAACTTCGTCAAGCTTTTCACCGGTAATGAAACCGATCTG
ACCTGGTTCAACAAAGAAATTGAAGCCGGACGCCCCTACTCTGCCGATCTCAAGTTGGTGGACGAAGACGTTCAGCGTTG
CCGCGCCAAGCTTGAAGCCATTGAAGTGGAAACCGGTTTGTCCATTGAATCCATCAAGGATATTAACCGCCGCATGAGCA
TCGGTGAAGCCAAGGCTCGCCGTGCCAAAAAAGAAATGGTTGAGGCCAACTTGCGTCTGGTAATTTCTATCGCCAAGAAA
TACACCAACCGCGGCCTGCAATTCCTGGATCTCATCCAGGAAGGTAATATCGGCCTGATGAAGGCGGTGGACAAGTTTGA
ATACCGTCGCGGTTACAAGTTCTCGACCTACGCGACCTGGTGGATCCGTCAGGCCATCACCCGCTCTATCGCGGATCAGG
CCCGTACCATCCGTATTCCGGTGCATATGATTGAGACCATTAACAAACTCAACCGTATCAGCCGCCAGATGCTGCAGGAA
ATGGGTCGCGAACCCTCACCTGAAGAACTGGCTGAGCGCATGGCCATGCCCGAGGACAAGATCCGTAAGGTACTGAAAAT
TGCCAAAGAGCCTATCTCCATGGAAACCCCAATCGGTGACGATGAAGATTCGCATCTGGGGGATTTCATCGAGGACACCA
CCCTCGAGCTGCCACTGGACAGCGCCACCAGCGAGAGCCTCAAGCAGGCTACCCACGAAGTGCTTGCCGGTTTGACTGCC
CGTGAGGCCAAGGTACTGCGCATGCGCTTTGGTATCGACATGAACACCGACCACACCCTCGAGGAAGTGGGCAAGCAGTT
CGATGTTACCCGTGAGCGTATCCGTCAGATTGAGGCCAAGGCACTGCGCAAACTGCGCCACCCAAGCCGCTCGGAAATCC
TCAAGTCATTCCTGGATGATTAA

Upstream 100 bases:

>100_bases
TCCCTCTGGCACAGCCATAGGCAGATGGCTATAATTGACGATTTGCGCGACTTTGTGGTCCGGCCACGGGTGCGCTCGTT
GTAACACTTACCCAAATTGG

Downstream 100 bases:

>100_bases
TCCTGACTGACACCACAAAGCCACCCTCGGGTGGCTTTGTTTTTTCTCGGTATTTTTAACCCGGCAACCGATTTGATTAC
CCAATAACCAATTGAACGCG

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MISMDHTPQSQLKMLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDIIQMINDMGIRVYEEAPDADEMMMSEDNTDEDAA
EEAAAALATVESELGRTTDPVRMYMREMGTVELLTREGEIVIAKRIEEGINTVQSSVAEYPQAISMILEQFDQFEADAIR
LSDIISGFVDPNEEDVGPTATHIGSELSEEELEDEDDMDEDEEEDEDGDSSDDDGAKGPDPEVARERFAALRAAYDNALK
VIEVKGRAHPESTRALFELGEIFKEFRLVPKQFDRLVKSMREMMDKVRVQERLIMKLCVEQAKMPKKNFVKLFTGNETDL
TWFNKEIEAGRPYSADLKLVDEDVQRCRAKLEAIEVETGLSIESIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKK
YTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQE
MGREPSPEELAERMAMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLKQATHEVLAGLTA
REAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEILKSFLDD

Sequences:

>Translated_620_residues
MISMDHTPQSQLKMLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDIIQMINDMGIRVYEEAPDADEMMMSEDNTDEDAA
EEAAAALATVESELGRTTDPVRMYMREMGTVELLTREGEIVIAKRIEEGINTVQSSVAEYPQAISMILEQFDQFEADAIR
LSDIISGFVDPNEEDVGPTATHIGSELSEEELEDEDDMDEDEEEDEDGDSSDDDGAKGPDPEVARERFAALRAAYDNALK
VIEVKGRAHPESTRALFELGEIFKEFRLVPKQFDRLVKSMREMMDKVRVQERLIMKLCVEQAKMPKKNFVKLFTGNETDL
TWFNKEIEAGRPYSADLKLVDEDVQRCRAKLEAIEVETGLSIESIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKK
YTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQE
MGREPSPEELAERMAMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLKQATHEVLAGLTA
REAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEILKSFLDD
>Mature_620_residues
MISMDHTPQSQLKMLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDIIQMINDMGIRVYEEAPDADEMMMSEDNTDEDAA
EEAAAALATVESELGRTTDPVRMYMREMGTVELLTREGEIVIAKRIEEGINTVQSSVAEYPQAISMILEQFDQFEADAIR
LSDIISGFVDPNEEDVGPTATHIGSELSEEELEDEDDMDEDEEEDEDGDSSDDDGAKGPDPEVARERFAALRAAYDNALK
VIEVKGRAHPESTRALFELGEIFKEFRLVPKQFDRLVKSMREMMDKVRVQERLIMKLCVEQAKMPKKNFVKLFTGNETDL
TWFNKEIEAGRPYSADLKLVDEDVQRCRAKLEAIEVETGLSIESIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKK
YTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQE
MGREPSPEELAERMAMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLKQATHEVLAGLTA
REAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEILKSFLDD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=618, Percent_Identity=78.3171521035599, Blast_Score=951, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=237, Percent_Identity=43.8818565400844, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1789871, Length=241, Percent_Identity=28.2157676348548, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.2178217821782, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 70701; Mature: 70701

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMDHTPQSQLKMLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDIIQMINDMGIRVYE
CCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
EAPDADEMMMSEDNTDEDAAEEAAAALATVESELGRTTDPVRMYMREMGTVELLTREGEI
CCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCE
VIAKRIEEGINTVQSSVAEYPQAISMILEQFDQFEADAIRLSDIISGFVDPNEEDVGPTA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
THIGSELSEEELEDEDDMDEDEEEDEDGDSSDDDGAKGPDPEVARERFAALRAAYDNALK
HHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEE
VIEVKGRAHPESTRALFELGEIFKEFRLVPKQFDRLVKSMREMMDKVRVQERLIMKLCVE
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKMPKKNFVKLFTGNETDLTWFNKEIEAGRPYSADLKLVDEDVQRCRAKLEAIEVETGL
HHHCCHHHEEEEECCCCCCCCEECCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SIESIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLM
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHH
KAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHH
MGREPSPEELAERMAMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLD
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEECCCC
SATSESLKQATHEVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAK
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALRKLRHPSRSEILKSFLDD
HHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MISMDHTPQSQLKMLLAKGKEQGYLTYAEVNDHLPADMVDSDQIEDIIQMINDMGIRVYE
CCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
EAPDADEMMMSEDNTDEDAAEEAAAALATVESELGRTTDPVRMYMREMGTVELLTREGEI
CCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCE
VIAKRIEEGINTVQSSVAEYPQAISMILEQFDQFEADAIRLSDIISGFVDPNEEDVGPTA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
THIGSELSEEELEDEDDMDEDEEEDEDGDSSDDDGAKGPDPEVARERFAALRAAYDNALK
HHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEE
VIEVKGRAHPESTRALFELGEIFKEFRLVPKQFDRLVKSMREMMDKVRVQERLIMKLCVE
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKMPKKNFVKLFTGNETDLTWFNKEIEAGRPYSADLKLVDEDVQRCRAKLEAIEVETGL
HHHCCHHHEEEEECCCCCCCCEECCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SIESIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLM
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHH
KAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHH
MGREPSPEELAERMAMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLD
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEECCCC
SATSESLKQATHEVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAK
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALRKLRHPSRSEILKSFLDD
HHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11272828 [H]