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The map label for this gene is pcnB [H]

Identifier: 119773915

GI number: 119773915

Start: 960154

End: 961428

Strand: Reverse

Name: pcnB [H]

Synonym: Sama_0778

Alternate gene names: 119773915

Gene position: 961428-960154 (Counterclockwise)

Preceding gene: 119773916

Following gene: 119773914

Centisome position: 22.33

GC content: 58.67

Gene sequence:

>1275_bases
GTGCTTTATCGCCTGAACAAGGCCGGTTACGAGGCCTTTTTGGTGGGTGGCGGTGTACGCGATCTGCTGCTGGGCCTGGA
GCCGAAAGACTTTGACGTGGTGACCAACGCCACCCCCGAAGACATCAAAAAGTTGTTCCGCAACTGCCGCCTGGTGGGTC
GCCGTTTCCGTTTGGCCCACATCGTATTTGGCCGTGACGTGATTGAAGTAGCGACCTTCCGTGGTCATCACGGCAATGAG
GATGGCGACAAGATTTCCAAGGCCAACGCCGAGGGACGCCTGCTGCGGGACAACGTGTACGGCAGCATCGATGAAGACGC
CGAACGCCGCGACTTTACCGTAAACGCCCTCTACTACGACATCAGCGATTACTCTATCCGCAGCTACGGCGGCGGTATGG
AAGATCTCAACGACGGTGTATTGCGCCTGATTGGCGATCCTGAGACCCGCTACCGCGAAGACCCGGTGCGGATGATCCGC
GCCGTGCGCTTTGCCACCAAGCTGGGCATGCGCATCGAAGAGGTAACAGCCGCGCCAATCCGCAAACTGGCACCGCTGCT
GCGCGATATTCCGGCCGCCCGCATGTACGAAGAAGTGCTCAAGCTGTTTTTTGCGGGCAAGGGTCAGGCAAACTATCAGA
TGATGCAGGAATATCAGCTATTTGCGCCGATTTTCCCGCTGCTGGCCGACACCATTGCCGACTCACCAAAGGGCCACTGT
GCCCGTATGGTGAACCTGGTGATGCGCAATACCGATATCCGGGTCATGGAAGACAAGCCAGTCACACCGGCCTTCTTCTA
TGCGGCCCTGCTGTGGTATCCGCTGAAAAGCCGCGCCGAAGAAATTGCCAACGAGAGTGGCCTCAGTCTGTACGATGCCT
ATTTTGCCGCCATGGGCGATGTGCTCGAGCAGCAATGCCAGACCATTTCGCTGCCCAAGCGCTTCAGTGTTCCGGCTCGG
GATATCTGGCAGATGCAGCTCAGATTTGAGCGCAGCCAGGGTACCCGCGCCTTTAAACTGATGGAAAGCCCCAAGTTCCG
CGCCGGTTATGATTTGCTGTTGATGCGCGCCGAAATAGAGGGTGGCAATCTGCAAAAGACCGCCAGCTGGTGGCAGTCGT
TTGTAGAAGCGCCGGAAGAAGAACGTGGCAATATCGCCCGCGGCGGTGCCAAGAGTGATGCTCCCCGCAACCGCAAACGC
CGCGTTCGCCGTCGCCCCCGCAGTGAAGGTGGCAGTGGCGAAGGAGCCAAGGCAAACACCGGCAGCGACAGCTGA

Upstream 100 bases:

>100_bases
TCAGCAGGGTGCCGACAATGCACAGGGCCTGAGTCTGAGCATCACGCCCCGAAATGCCCACACCATCTCGCGCAAAAACA
TCAGTGAAAATGCGCTCAAG

Downstream 100 bases:

>100_bases
ATATGACCCAGGTTTATGTGGCTCTCGGCGCCAATCTCGCCGAGCCCATCAGCCAACTGAACAGGGCCTGCGAGGCCCTG
TTGTCCTTGGCAGCCCCAGG

Product: tRNA nucleotidyltransferase (CCA-adding enzyme)

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MLYRLNKAGYEAFLVGGGVRDLLLGLEPKDFDVVTNATPEDIKKLFRNCRLVGRRFRLAHIVFGRDVIEVATFRGHHGNE
DGDKISKANAEGRLLRDNVYGSIDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLNDGVLRLIGDPETRYREDPVRMIR
AVRFATKLGMRIEEVTAAPIRKLAPLLRDIPAARMYEEVLKLFFAGKGQANYQMMQEYQLFAPIFPLLADTIADSPKGHC
ARMVNLVMRNTDIRVMEDKPVTPAFFYAALLWYPLKSRAEEIANESGLSLYDAYFAAMGDVLEQQCQTISLPKRFSVPAR
DIWQMQLRFERSQGTRAFKLMESPKFRAGYDLLLMRAEIEGGNLQKTASWWQSFVEAPEEERGNIARGGAKSDAPRNRKR
RVRRRPRSEGGSGEGAKANTGSDS

Sequences:

>Translated_424_residues
MLYRLNKAGYEAFLVGGGVRDLLLGLEPKDFDVVTNATPEDIKKLFRNCRLVGRRFRLAHIVFGRDVIEVATFRGHHGNE
DGDKISKANAEGRLLRDNVYGSIDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLNDGVLRLIGDPETRYREDPVRMIR
AVRFATKLGMRIEEVTAAPIRKLAPLLRDIPAARMYEEVLKLFFAGKGQANYQMMQEYQLFAPIFPLLADTIADSPKGHC
ARMVNLVMRNTDIRVMEDKPVTPAFFYAALLWYPLKSRAEEIANESGLSLYDAYFAAMGDVLEQQCQTISLPKRFSVPAR
DIWQMQLRFERSQGTRAFKLMESPKFRAGYDLLLMRAEIEGGNLQKTASWWQSFVEAPEEERGNIARGGAKSDAPRNRKR
RVRRRPRSEGGSGEGAKANTGSDS
>Mature_424_residues
MLYRLNKAGYEAFLVGGGVRDLLLGLEPKDFDVVTNATPEDIKKLFRNCRLVGRRFRLAHIVFGRDVIEVATFRGHHGNE
DGDKISKANAEGRLLRDNVYGSIDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLNDGVLRLIGDPETRYREDPVRMIR
AVRFATKLGMRIEEVTAAPIRKLAPLLRDIPAARMYEEVLKLFFAGKGQANYQMMQEYQLFAPIFPLLADTIADSPKGHC
ARMVNLVMRNTDIRVMEDKPVTPAFFYAALLWYPLKSRAEEIANESGLSLYDAYFAAMGDVLEQQCQTISLPKRFSVPAR
DIWQMQLRFERSQGTRAFKLMESPKFRAGYDLLLMRAEIEGGNLQKTASWWQSFVEAPEEERGNIARGGAKSDAPRNRKR
RVRRRPRSEGGSGEGAKANTGSDS

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=163, Percent_Identity=30.6748466257669, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI87081691, Length=386, Percent_Identity=59.0673575129534, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71995920, Length=215, Percent_Identity=26.5116279069767, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281360112, Length=229, Percent_Identity=27.0742358078603, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357337, Length=229, Percent_Identity=27.0742358078603, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 48063; Mature: 48063

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYRLNKAGYEAFLVGGGVRDLLLGLEPKDFDVVTNATPEDIKKLFRNCRLVGRRFRLAH
CEEECCCCCCEEEEECCCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IVFGRDVIEVATFRGHHGNEDGDKISKANAEGRLLRDNVYGSIDEDAERRDFTVNALYYD
HHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEE
ISDYSIRSYGGGMEDLNDGVLRLIGDPETRYREDPVRMIRAVRFATKLGMRIEEVTAAPI
CCCCCHHHCCCCHHHHCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
RKLAPLLRDIPAARMYEEVLKLFFAGKGQANYQMMQEYQLFAPIFPLLADTIADSPKGHC
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ARMVNLVMRNTDIRVMEDKPVTPAFFYAALLWYPLKSRAEEIANESGLSLYDAYFAAMGD
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VLEQQCQTISLPKRFSVPARDIWQMQLRFERSQGTRAFKLMESPKFRAGYDLLLMRAEIE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEEEEEEEECC
GGNLQKTASWWQSFVEAPEEERGNIARGGAKSDAPRNRKRRVRRRPRSEGGSGEGAKANT
CCCCHHHHHHHHHHHCCCHHHCCCEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
GSDS
CCCC
>Mature Secondary Structure
MLYRLNKAGYEAFLVGGGVRDLLLGLEPKDFDVVTNATPEDIKKLFRNCRLVGRRFRLAH
CEEECCCCCCEEEEECCCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IVFGRDVIEVATFRGHHGNEDGDKISKANAEGRLLRDNVYGSIDEDAERRDFTVNALYYD
HHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEE
ISDYSIRSYGGGMEDLNDGVLRLIGDPETRYREDPVRMIRAVRFATKLGMRIEEVTAAPI
CCCCCHHHCCCCHHHHCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
RKLAPLLRDIPAARMYEEVLKLFFAGKGQANYQMMQEYQLFAPIFPLLADTIADSPKGHC
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ARMVNLVMRNTDIRVMEDKPVTPAFFYAALLWYPLKSRAEEIANESGLSLYDAYFAAMGD
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VLEQQCQTISLPKRFSVPARDIWQMQLRFERSQGTRAFKLMESPKFRAGYDLLLMRAEIE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEEEEEEEECC
GGNLQKTASWWQSFVEAPEEERGNIARGGAKSDAPRNRKRRVRRRPRSEGGSGEGAKANT
CCCCHHHHHHHHHHHCCCHHHCCCEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
GSDS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]