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The map label for this gene is ywbI [H]

Identifier: 119773724

GI number: 119773724

Start: 732315

End: 733223

Strand: Reverse

Name: ywbI [H]

Synonym: Sama_0586

Alternate gene names: 119773724

Gene position: 733223-732315 (Counterclockwise)

Preceding gene: 119773725

Following gene: 119773717

Centisome position: 17.03

GC content: 56.99

Gene sequence:

>909_bases
ATGAACATTCCAATCAAGAACTTGCACTGTTTTGTGACCCTTGCCGAGACGGGCAGTTTTACCCGCGCGGCCGAAAAACT
GCACCTGACTCAGCCCACGCTGAGCAAGATGCTGCAAAGACTGGAAGACCATTGGCAGCAGCAGCTCATCATCCGCAACA
ATCAAAAGCTGTCACTGACTCAGGCGGGCGAACTCTTGCTTGAACACGCCAGAGAAATGCTTGGGCAATGGCATTTATTG
GAAGAAGACTTATCAAATCTTCGCGGGGTACAACGGGGATACTTGCGTCTGGGAGTGTGCCCCATGATGAGCAGCCTGGT
GATTGAACTCCTCACTGCGTTTCGAGCAAGATATCCCGGGGTCAGATTGGAGATGTTCGAGTACGGCGGCTTCGGCTGTG
AAAAGGCTCTGCTGGCAAACAATCTGGATATTGCCTTTACCGCCCTGCCTGCAACCCACGCCGATGAGCTGACCGCGCAG
CCGTTGACGGCTTATCCCCTGCTCGCCTGCCTGCCGGCAGGCCACAAGCTTGCGAACAAAGCCGCCCTCGACTGGTGCGA
CTTTGGCTCCCATCCCTTTATCATGTACAACCAGGATTTTGCGCTGGCAAAGCTGCTCGACAGCCTGGCAAGAAACGCCG
GAGTAGAGCTGAATGTCGCCTATCGCAGCGGCCAGTGGGACTTTTTGGCGAGCATGGTGGAGGCCAACATGGGGCTGGCC
ATATTGCCCGCGCCCATTTGCGAAAAATTAACCGACAAGGCGCTGTGCTTCAGGCCATTAAGCGGCGGACACACCTGGGA
CCTGGCACTCATCTGGCGCAGTCATCTGCACCTGACCCCTGCGGCGGCCGCCTTTCTGGCCCTCAGTCGTGATAGGGGCC
AAGCCACTGCGCGTCCGGCGGCAGAATAA

Upstream 100 bases:

>100_bases
TTTCATCAAAATTCCTCCAAAGCTGAGATGCAGCTCAAGGTATCTCCCGGCCATCAATAAGTAAAATGAATCTATTCCAT
CACTTCCATTCCATATTGGC

Downstream 100 bases:

>100_bases
GCAGATTACACCTGAGCTTGTGCACCAGCTGCTCTGCGGTGCTGCCAATGGAAGCGCCGAATATACCCTCCCTTTGGCGT
GCTCCCATCACCAGGTATTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNIPIKNLHCFVTLAETGSFTRAAEKLHLTQPTLSKMLQRLEDHWQQQLIIRNNQKLSLTQAGELLLEHAREMLGQWHLL
EEDLSNLRGVQRGYLRLGVCPMMSSLVIELLTAFRARYPGVRLEMFEYGGFGCEKALLANNLDIAFTALPATHADELTAQ
PLTAYPLLACLPAGHKLANKAALDWCDFGSHPFIMYNQDFALAKLLDSLARNAGVELNVAYRSGQWDFLASMVEANMGLA
ILPAPICEKLTDKALCFRPLSGGHTWDLALIWRSHLHLTPAAAAFLALSRDRGQATARPAAE

Sequences:

>Translated_302_residues
MNIPIKNLHCFVTLAETGSFTRAAEKLHLTQPTLSKMLQRLEDHWQQQLIIRNNQKLSLTQAGELLLEHAREMLGQWHLL
EEDLSNLRGVQRGYLRLGVCPMMSSLVIELLTAFRARYPGVRLEMFEYGGFGCEKALLANNLDIAFTALPATHADELTAQ
PLTAYPLLACLPAGHKLANKAALDWCDFGSHPFIMYNQDFALAKLLDSLARNAGVELNVAYRSGQWDFLASMVEANMGLA
ILPAPICEKLTDKALCFRPLSGGHTWDLALIWRSHLHLTPAAAAFLALSRDRGQATARPAAE
>Mature_302_residues
MNIPIKNLHCFVTLAETGSFTRAAEKLHLTQPTLSKMLQRLEDHWQQQLIIRNNQKLSLTQAGELLLEHAREMLGQWHLL
EEDLSNLRGVQRGYLRLGVCPMMSSLVIELLTAFRARYPGVRLEMFEYGGFGCEKALLANNLDIAFTALPATHADELTAQ
PLTAYPLLACLPAGHKLANKAALDWCDFGSHPFIMYNQDFALAKLLDSLARNAGVELNVAYRSGQWDFLASMVEANMGLA
ILPAPICEKLTDKALCFRPLSGGHTWDLALIWRSHLHLTPAAAAFLALSRDRGQATARPAAE

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=289, Percent_Identity=28.0276816608997, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI145693105, Length=293, Percent_Identity=26.6211604095563, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787879, Length=294, Percent_Identity=27.891156462585, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790399, Length=241, Percent_Identity=27.8008298755187, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=25.5972696245734, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788748, Length=299, Percent_Identity=25.4180602006689, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788297, Length=143, Percent_Identity=34.2657342657343, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789204, Length=291, Percent_Identity=27.4914089347079, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=26.5060240963855, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790262, Length=293, Percent_Identity=26.6211604095563, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789639, Length=194, Percent_Identity=27.319587628866, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787601, Length=305, Percent_Identity=24.9180327868852, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786401, Length=163, Percent_Identity=30.0613496932515, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367136, Length=295, Percent_Identity=22.7118644067797, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787530, Length=241, Percent_Identity=22.8215767634855, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786713, Length=265, Percent_Identity=28.6792452830189, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=235, Percent_Identity=26.3829787234043, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=271, Percent_Identity=23.2472324723247, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789506, Length=296, Percent_Identity=21.2837837837838, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081904, Length=99, Percent_Identity=37.3737373737374, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33527; Mature: 33527

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPIKNLHCFVTLAETGSFTRAAEKLHLTQPTLSKMLQRLEDHWQQQLIIRNNQKLSLT
CCCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCEEEHH
QAGELLLEHAREMLGQWHLLEEDLSNLRGVQRGYLRLGVCPMMSSLVIELLTAFRARYPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VRLEMFEYGGFGCEKALLANNLDIAFTALPATHADELTAQPLTAYPLLACLPAGHKLANK
CEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCHHHCCCCCHHHHHHHHCCCCHHHHHH
AALDWCDFGSHPFIMYNQDFALAKLLDSLARNAGVELNVAYRSGQWDFLASMVEANMGLA
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCEE
ILPAPICEKLTDKALCFRPLSGGHTWDLALIWRSHLHLTPAAAAFLALSRDRGQATARPA
EECHHHHHHHCCHHHEEECCCCCCEEEHHEEEHHHCCCCHHHHHHHHHHCCCCCCCCCCC
AE
CC
>Mature Secondary Structure
MNIPIKNLHCFVTLAETGSFTRAAEKLHLTQPTLSKMLQRLEDHWQQQLIIRNNQKLSLT
CCCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCEEEHH
QAGELLLEHAREMLGQWHLLEEDLSNLRGVQRGYLRLGVCPMMSSLVIELLTAFRARYPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VRLEMFEYGGFGCEKALLANNLDIAFTALPATHADELTAQPLTAYPLLACLPAGHKLANK
CEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCHHHCCCCCHHHHHHHHCCCCHHHHHH
AALDWCDFGSHPFIMYNQDFALAKLLDSLARNAGVELNVAYRSGQWDFLASMVEANMGLA
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCEE
ILPAPICEKLTDKALCFRPLSGGHTWDLALIWRSHLHLTPAAAAFLALSRDRGQATARPA
EECHHHHHHHCCHHHEEECCCCCCEEEHHEEEHHHCCCCHHHHHHHHHHCCCCCCCCCCC
AE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]