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The map label for this gene is phoA [H]

Identifier: 119773642

GI number: 119773642

Start: 615538

End: 617202

Strand: Reverse

Name: phoA [H]

Synonym: Sama_0502

Alternate gene names: 119773642

Gene position: 617202-615538 (Counterclockwise)

Preceding gene: 119773643

Following gene: 119773640

Centisome position: 14.33

GC content: 56.34

Gene sequence:

>1665_bases
ATGAAACATAAAAGCCTGCTGGCAGTAGCCATCGCAGCCGCACTGATGAGCGGTTGCAACGACGACAAAACGGTTGAAGT
GATCAAGGAAGTGGAAGTCATCAAGGAAGTGGCTCCCGAGGAAATCACCTCGGTGAAAAACGTCATCCTGATGATCGGCG
ATGGTATGGGTGCCCAGCAGGTTGGCCTACTCGAAGAATATGCCCGCCGCGCTCCGCACTCCACCTACAACAGCAAAGGG
AACCAAACTGCCCTCAAGCGTTTGGGTGATGCCGGCCAGATGGGTCTGTCGCTCAACGGCCCCGATGGCAGCCTGGTAGT
GGATTCAGCCTGCTCAGCCAGCCAGTTGGCTACCGGCAAGGCCGCGGGTTCAGAGATGATTGGTTTGGATGCTCAGGGCT
ATGTAGTTGAAACCATCCTTGAGAAGGCAAAAGCCAAAGGCAAGGCAACCGGTTTGGTATCCGATACCCGGCTGACTCAC
GCAACACCCGCCGCCTTTGCCGCCCACCAGCCACATCGCAGCTATGAATCAGCCATTGCCGCCGAGCTTATCGACAAGGC
CGTGGTGGACGTCATGCTCTCCGGTGGTGCCCGTGAATTTATTCCTCAGGATGCCAGCAGCAACAATGCGGTCAAAGCCG
AACTGGCCGCCCTGGGCGCCGTGTCGTCTGTGGTAAAAAAATCCAAGCGTAAAGACGACCGCAACCTGGTGAAAGAAGCC
AAGGAAGCCAAGGGCTACCAGCTGGCGTTCGACAAGGCACAGCTCAATGCCGCCGACGGTGACAAGCTGCTGGGTCTGTT
TGCCAACTCTGGCATGATGGATGCCATTGCCTACAAGGCCTGTCAGGAAGCCGGGGATTGCAAAGAGCCTTCGCTGCGTG
AAATGACTCTCAAAGCGCTGGATATTCTGTCCAAGGACGAAGACGGCTTCTTCCTGATGATTGAAGGTGGCCAGATTGAC
TGGGCCGGCCACATCAACGACGCAGGCTGGATGCTGCACGAACTGCTGCGCTTCGACGAAGCTGTGGATGCAGTATATGA
ATGGGTTAAAGACCGCAACGATACCCTGGTGATTGTGACCGCCGACCACGAAACAGGCAGCTTCGGTCTGAGCTACAGCC
GCAAAGACCTGCCCGGCGCCCAGAGCCTGCCCGGTGATGGAATGCAGGGCGCCGACTATCAGCCCAACTTCAACTTTGGC
AGTCTGGACAAACTGGACCGCCTCTATGCGCAAAGCGGCACCTTCTACAACATGATGGACATGGTCAGCGCCGACTGGGC
CTTCGATAACTCCACCGGTGACGAATGGGCCAGTGCCATCAACCACTATAGCGACTTCAAAGTAACACCAGAAGAGGCCG
CGCCGGTTGCCCTGCGTGAAGCCAATGAGTATTGGGTCGATGGCCACAGCTATTTATCTGCCACCGTGTTCCCTAAGGTC
AATGACTTCAAGGAGTTCTACGTTTACGCAGACGAAGTACACGCCAACCTGATTGGCCGAGCCCTGGCTGCGGATCAAAA
CGTGGTTTGGGGTACAGGTACCCACACAGCCGCGCCAGTGCCCGTTTACGCTTTCGGTCCTTACGGTGTAACCAAGCAGT
TCTCCACTATGCAGCACCACACCGAAGTGGGTCAGAAAATGATGGCCGCACTGCTGGGTGAATAA

Upstream 100 bases:

>100_bases
AAACTGACACAAAACTTTCGTCATTTTGTCATCTGCTCTGGTCAGACTTTCGGGCGTTTAAGTCACGCCCTACATATTAG
AAAGTCAAAAGAGATCAAGC

Downstream 100 bases:

>100_bases
CCAAGCTCTGTGAGTACAAACACCCCATAAAAAAGCCGCCACTGGGCGGCTTTTTTATTTGGCTCATCAAGGCTGGCTGC
AAACATCAGGGACTTGTTGA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKHKSLLAVAIAAALMSGCNDDKTVEVIKEVEVIKEVAPEEITSVKNVILMIGDGMGAQQVGLLEEYARRAPHSTYNSKG
NQTALKRLGDAGQMGLSLNGPDGSLVVDSACSASQLATGKAAGSEMIGLDAQGYVVETILEKAKAKGKATGLVSDTRLTH
ATPAAFAAHQPHRSYESAIAAELIDKAVVDVMLSGGAREFIPQDASSNNAVKAELAALGAVSSVVKKSKRKDDRNLVKEA
KEAKGYQLAFDKAQLNAADGDKLLGLFANSGMMDAIAYKACQEAGDCKEPSLREMTLKALDILSKDEDGFFLMIEGGQID
WAGHINDAGWMLHELLRFDEAVDAVYEWVKDRNDTLVIVTADHETGSFGLSYSRKDLPGAQSLPGDGMQGADYQPNFNFG
SLDKLDRLYAQSGTFYNMMDMVSADWAFDNSTGDEWASAINHYSDFKVTPEEAAPVALREANEYWVDGHSYLSATVFPKV
NDFKEFYVYADEVHANLIGRALAADQNVVWGTGTHTAAPVPVYAFGPYGVTKQFSTMQHHTEVGQKMMAALLGE

Sequences:

>Translated_554_residues
MKHKSLLAVAIAAALMSGCNDDKTVEVIKEVEVIKEVAPEEITSVKNVILMIGDGMGAQQVGLLEEYARRAPHSTYNSKG
NQTALKRLGDAGQMGLSLNGPDGSLVVDSACSASQLATGKAAGSEMIGLDAQGYVVETILEKAKAKGKATGLVSDTRLTH
ATPAAFAAHQPHRSYESAIAAELIDKAVVDVMLSGGAREFIPQDASSNNAVKAELAALGAVSSVVKKSKRKDDRNLVKEA
KEAKGYQLAFDKAQLNAADGDKLLGLFANSGMMDAIAYKACQEAGDCKEPSLREMTLKALDILSKDEDGFFLMIEGGQID
WAGHINDAGWMLHELLRFDEAVDAVYEWVKDRNDTLVIVTADHETGSFGLSYSRKDLPGAQSLPGDGMQGADYQPNFNFG
SLDKLDRLYAQSGTFYNMMDMVSADWAFDNSTGDEWASAINHYSDFKVTPEEAAPVALREANEYWVDGHSYLSATVFPKV
NDFKEFYVYADEVHANLIGRALAADQNVVWGTGTHTAAPVPVYAFGPYGVTKQFSTMQHHTEVGQKMMAALLGE
>Mature_554_residues
MKHKSLLAVAIAAALMSGCNDDKTVEVIKEVEVIKEVAPEEITSVKNVILMIGDGMGAQQVGLLEEYARRAPHSTYNSKG
NQTALKRLGDAGQMGLSLNGPDGSLVVDSACSASQLATGKAAGSEMIGLDAQGYVVETILEKAKAKGKATGLVSDTRLTH
ATPAAFAAHQPHRSYESAIAAELIDKAVVDVMLSGGAREFIPQDASSNNAVKAELAALGAVSSVVKKSKRKDDRNLVKEA
KEAKGYQLAFDKAQLNAADGDKLLGLFANSGMMDAIAYKACQEAGDCKEPSLREMTLKALDILSKDEDGFFLMIEGGQID
WAGHINDAGWMLHELLRFDEAVDAVYEWVKDRNDTLVIVTADHETGSFGLSYSRKDLPGAQSLPGDGMQGADYQPNFNFG
SLDKLDRLYAQSGTFYNMMDMVSADWAFDNSTGDEWASAINHYSDFKVTPEEAAPVALREANEYWVDGHSYLSATVFPKV
NDFKEFYVYADEVHANLIGRALAADQNVVWGTGTHTAAPVPVYAFGPYGVTKQFSTMQHHTEVGQKMMAALLGE

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=387, Percent_Identity=30.2325581395349, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI116734717, Length=350, Percent_Identity=30.2857142857143, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157266296, Length=365, Percent_Identity=30.1369863013699, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI94721246, Length=365, Percent_Identity=29.8630136986301, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI294660770, Length=346, Percent_Identity=29.4797687861272, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI294660772, Length=290, Percent_Identity=30.6896551724138, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI48994877, Length=359, Percent_Identity=34.2618384401114, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6320689, Length=350, Percent_Identity=33.4285714285714, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24651554, Length=391, Percent_Identity=32.2250639386189, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585213, Length=364, Percent_Identity=32.6923076923077, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355981, Length=393, Percent_Identity=30.0254452926209, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24651556, Length=311, Percent_Identity=34.7266881028939, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21358067, Length=366, Percent_Identity=30.327868852459, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21355151, Length=355, Percent_Identity=31.2676056338028, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19921912, Length=404, Percent_Identity=29.7029702970297, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI18859923, Length=395, Percent_Identity=30.379746835443, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24657827, Length=356, Percent_Identity=31.4606741573034, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355149, Length=372, Percent_Identity=29.5698924731183, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI85725200, Length=388, Percent_Identity=29.1237113402062, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI85815833, Length=388, Percent_Identity=29.1237113402062, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24657835, Length=352, Percent_Identity=28.9772727272727, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24657842, Length=373, Percent_Identity=28.9544235924933, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 59663; Mature: 59663

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKSLLAVAIAAALMSGCNDDKTVEVIKEVEVIKEVAPEEITSVKNVILMIGDGMGAQQ
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHEEEEEECCCCHHH
VGLLEEYARRAPHSTYNSKGNQTALKRLGDAGQMGLSLNGPDGSLVVDSACSASQLATGK
HHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEEECCCCHHHHCCCC
AAGSEMIGLDAQGYVVETILEKAKAKGKATGLVSDTRLTHATPAAFAAHQPHRSYESAIA
CCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCHHHHCCCCCHHHHHHHH
AELIDKAVVDVMLSGGAREFIPQDASSNNAVKAELAALGAVSSVVKKSKRKDDRNLVKEA
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KEAKGYQLAFDKAQLNAADGDKLLGLFANSGMMDAIAYKACQEAGDCKEPSLREMTLKAL
HHCCCCEEEECHHHCCCCCCCCEEEEHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
DILSKDEDGFFLMIEGGQIDWAGHINDAGWMLHELLRFDEAVDAVYEWVKDRNDTLVIVT
HHHCCCCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ADHETGSFGLSYSRKDLPGAQSLPGDGMQGADYQPNFNFGSLDKLDRLYAQSGTFYNMMD
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
MVSADWAFDNSTGDEWASAINHYSDFKVTPEEAAPVALREANEYWVDGHSYLSATVFPKV
HHHCCCCCCCCCHHHHHHHHHHCCCCEECCHHCCCCCEECCCCEEECCCHHEEEEECCCC
NDFKEFYVYADEVHANLIGRALAADQNVVWGTGTHTAAPVPVYAFGPYGVTKQFSTMQHH
CCHHHHHEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHH
TEVGQKMMAALLGE
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKHKSLLAVAIAAALMSGCNDDKTVEVIKEVEVIKEVAPEEITSVKNVILMIGDGMGAQQ
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHEEEEEECCCCHHH
VGLLEEYARRAPHSTYNSKGNQTALKRLGDAGQMGLSLNGPDGSLVVDSACSASQLATGK
HHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEEECCCCHHHHCCCC
AAGSEMIGLDAQGYVVETILEKAKAKGKATGLVSDTRLTHATPAAFAAHQPHRSYESAIA
CCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCHHHHCCCCCHHHHHHHH
AELIDKAVVDVMLSGGAREFIPQDASSNNAVKAELAALGAVSSVVKKSKRKDDRNLVKEA
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KEAKGYQLAFDKAQLNAADGDKLLGLFANSGMMDAIAYKACQEAGDCKEPSLREMTLKAL
HHCCCCEEEECHHHCCCCCCCCEEEEHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
DILSKDEDGFFLMIEGGQIDWAGHINDAGWMLHELLRFDEAVDAVYEWVKDRNDTLVIVT
HHHCCCCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
ADHETGSFGLSYSRKDLPGAQSLPGDGMQGADYQPNFNFGSLDKLDRLYAQSGTFYNMMD
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
MVSADWAFDNSTGDEWASAINHYSDFKVTPEEAAPVALREANEYWVDGHSYLSATVFPKV
HHHCCCCCCCCCHHHHHHHHHHCCCCEECCHHCCCCCEECCCCEEECCCHHEEEEECCCC
NDFKEFYVYADEVHANLIGRALAADQNVVWGTGTHTAAPVPVYAFGPYGVTKQFSTMQHH
CCHHHHHEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHH
TEVGQKMMAALLGE
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]