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The map label for this gene is opuE [H]

Identifier: 119773632

GI number: 119773632

Start: 604192

End: 605688

Strand: Reverse

Name: opuE [H]

Synonym: Sama_0492

Alternate gene names: 119773632

Gene position: 605688-604192 (Counterclockwise)

Preceding gene: 119773640

Following gene: 119773631

Centisome position: 14.07

GC content: 55.44

Gene sequence:

>1497_bases
ATGGAATACTCCAGCTATATTTCGCTGGCACTCTACTTTATTGCCATGTTGGCAATAGGGCTGTTTGCCTATCGTAATTC
CACCGACGATCTTGCGGGTTATATGCTTGGCGGGCGTCAGGTCAGCCCGCACGTCACCGCCCTGTCTGCCGGTGCATCCG
ATATGAGCGGCTGGATGCTGATGGGGCTGCCCGGCGCCATGTTTACCATGGGCTATGATGCTCTGTGGATTGCCGCTGGC
CTGCTGCTGGGGGCGCTGCTCAATTATCTGCTGGTTGCACCCAGACTCAGAGTGTTTACTGAAGCAGCTGATGACGCCCT
GACACTGCCAGACTTTTTCAGCAAGCGCTTCAATAAAGACAACGGCTCGGTGCGGATGATTTCAGCGGCGGTGATTATCC
TGTTTTTCACCCTGTATACCTCAGCCGGACTGGTCGCCGGGGGTAAGTTTTTTGAGTCGGCTTTTGGACTGGGCTATCAA
ACCGGCTTGTTGCTGACTGTGTCTGTGGTGGTGGCATACACCCTGCTCGGCGGATTCCTGGCGGTCAGCCTGACTGACTT
TGTTCAGGGCTGTATCATGTTTCTTGCACTGGTGTTGGTGCCGTTGGTTGCGCTTCAGGAATTCAACAGCAGTACCGACA
TGCTGGCGCAGGCCAGCCGCAGCATTACCCCATTGGCCGACTCTTTTGAGCACATGGGGATTCTGGCGATAGTCTCAAGC
CTCGCATGGGGGCTGGGTTACTTTGGGCAACCGCATATTATTGTGCGATTTATGGCAATTCGGTCGGTGAAGGACATTGC
TGTTGCCCGCAATATCGGCATGAGCTGGATGTTGGTCACCATATTAGGCGCGCTGGCAACCGGACTTATCGGCGTGGCTT
ACGTGAATAAATTCAAGCCGGGACTGGACGATGGCGAGACCATCTTCATTTTGTTCGCTGAAATCCTGTTTCATCCGCTC
ATCAGTGGGTTCCTGCTGGCAGCCATTCTGGCGGCCATCATGAGCACCATTTCCTCGCAGTTACTGGTGTCATCAAGCTC
TCTGTCGGAGGATGTGTACCGCGCCCTGTTTCACAAAGAGATGGACCAGAAAGCCACGGTTACGGCGGGACGTATCGGTG
TGCTGGCCGTGGCGGCGGTGGCAACGCTGCTGGCGCTGGACAATAACGCCAGCATCCTCTCGCTGGTGAGCAATGCCTGG
GCCGGTTTTGGCGCCGCCTTCGGCCCCCTCGTACTCCTGAGTTTATTCTGGCCCCGCATGACCCACAGCGGCGCAGTAGC
AGGCATACTCTGTGGCGCAGGCACTGTGTTGGTGTGGAGCCAGGTTCCACTGCTCGAAAACGGCTTACCCTTATCCAGTC
TCATTTATGAGATTGTGCCCGGGTTTATCGTAAGCACTCTCGCCATAGTACTGGTGAGTTATGCAGACCAGGCCCCCTGT
AAATCGACTCAGAAAGCCTTCAAGCTCAGCCGGGATAGGCTGAAACAAGTCAGCTGA

Upstream 100 bases:

>100_bases
CAATTAGCCTGCTTTTGGCTAATTCTCTCAAGTTATATATTTCCTTGGCTATCAGCCAATATCAAAAATATACTTCCTAC
AAAAATCAGGTAATTTTTAA

Downstream 100 bases:

>100_bases
TCCCACACAGGCCCGCCCCATGCGGGCCTTTTGCTATTTAACAGCGCGAACAGTCTTTTAGCGGTATGGGCTACCACCGC
CATGGCAAATTTTCCCAATC

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MEYSSYISLALYFIAMLAIGLFAYRNSTDDLAGYMLGGRQVSPHVTALSAGASDMSGWMLMGLPGAMFTMGYDALWIAAG
LLLGALLNYLLVAPRLRVFTEAADDALTLPDFFSKRFNKDNGSVRMISAAVIILFFTLYTSAGLVAGGKFFESAFGLGYQ
TGLLLTVSVVVAYTLLGGFLAVSLTDFVQGCIMFLALVLVPLVALQEFNSSTDMLAQASRSITPLADSFEHMGILAIVSS
LAWGLGYFGQPHIIVRFMAIRSVKDIAVARNIGMSWMLVTILGALATGLIGVAYVNKFKPGLDDGETIFILFAEILFHPL
ISGFLLAAILAAIMSTISSQLLVSSSSLSEDVYRALFHKEMDQKATVTAGRIGVLAVAAVATLLALDNNASILSLVSNAW
AGFGAAFGPLVLLSLFWPRMTHSGAVAGILCGAGTVLVWSQVPLLENGLPLSSLIYEIVPGFIVSTLAIVLVSYADQAPC
KSTQKAFKLSRDRLKQVS

Sequences:

>Translated_498_residues
MEYSSYISLALYFIAMLAIGLFAYRNSTDDLAGYMLGGRQVSPHVTALSAGASDMSGWMLMGLPGAMFTMGYDALWIAAG
LLLGALLNYLLVAPRLRVFTEAADDALTLPDFFSKRFNKDNGSVRMISAAVIILFFTLYTSAGLVAGGKFFESAFGLGYQ
TGLLLTVSVVVAYTLLGGFLAVSLTDFVQGCIMFLALVLVPLVALQEFNSSTDMLAQASRSITPLADSFEHMGILAIVSS
LAWGLGYFGQPHIIVRFMAIRSVKDIAVARNIGMSWMLVTILGALATGLIGVAYVNKFKPGLDDGETIFILFAEILFHPL
ISGFLLAAILAAIMSTISSQLLVSSSSLSEDVYRALFHKEMDQKATVTAGRIGVLAVAAVATLLALDNNASILSLVSNAW
AGFGAAFGPLVLLSLFWPRMTHSGAVAGILCGAGTVLVWSQVPLLENGLPLSSLIYEIVPGFIVSTLAIVLVSYADQAPC
KSTQKAFKLSRDRLKQVS
>Mature_498_residues
MEYSSYISLALYFIAMLAIGLFAYRNSTDDLAGYMLGGRQVSPHVTALSAGASDMSGWMLMGLPGAMFTMGYDALWIAAG
LLLGALLNYLLVAPRLRVFTEAADDALTLPDFFSKRFNKDNGSVRMISAAVIILFFTLYTSAGLVAGGKFFESAFGLGYQ
TGLLLTVSVVVAYTLLGGFLAVSLTDFVQGCIMFLALVLVPLVALQEFNSSTDMLAQASRSITPLADSFEHMGILAIVSS
LAWGLGYFGQPHIIVRFMAIRSVKDIAVARNIGMSWMLVTILGALATGLIGVAYVNKFKPGLDDGETIFILFAEILFHPL
ISGFLLAAILAAIMSTISSQLLVSSSSLSEDVYRALFHKEMDQKATVTAGRIGVLAVAAVATLLALDNNASILSLVSNAW
AGFGAAFGPLVLLSLFWPRMTHSGAVAGILCGAGTVLVWSQVPLLENGLPLSSLIYEIVPGFIVSTLAIVLVSYADQAPC
KSTQKAFKLSRDRLKQVS

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=502, Percent_Identity=49.800796812749, Blast_Score=487, Evalue=1e-138,
Organism=Homo sapiens, GI14140236, Length=473, Percent_Identity=22.8329809725159, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4507031, Length=463, Percent_Identity=21.8142548596112, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1787251, Length=486, Percent_Identity=48.9711934156379, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1790503, Length=421, Percent_Identity=26.3657957244656, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082237, Length=443, Percent_Identity=26.6365688487585, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17539284, Length=413, Percent_Identity=22.2760290556901, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24645928, Length=388, Percent_Identity=24.2268041237113, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648033, Length=392, Percent_Identity=22.9591836734694, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21356865, Length=392, Percent_Identity=22.9591836734694, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53008; Mature: 53008

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSSYISLALYFIAMLAIGLFAYRNSTDDLAGYMLGGRQVSPHVTALSAGASDMSGWML
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHEECCCCCCCCEEE
MGLPGAMFTMGYDALWIAAGLLLGALLNYLLVAPRLRVFTEAADDALTLPDFFSKRFNKD
ECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
NGSVRMISAAVIILFFTLYTSAGLVAGGKFFESAFGLGYQTGLLLTVSVVVAYTLLGGFL
CCCCHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AVSLTDFVQGCIMFLALVLVPLVALQEFNSSTDMLAQASRSITPLADSFEHMGILAIVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LAWGLGYFGQPHIIVRFMAIRSVKDIAVARNIGMSWMLVTILGALATGLIGVAYVNKFKP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLDDGETIFILFAEILFHPLISGFLLAAILAAIMSTISSQLLVSSSSLSEDVYRALFHKE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MDQKATVTAGRIGVLAVAAVATLLALDNNASILSLVSNAWAGFGAAFGPLVLLSLFWPRM
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
THSGAVAGILCGAGTVLVWSQVPLLENGLPLSSLIYEIVPGFIVSTLAIVLVSYADQAPC
CCCCCHHHHHHCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSTQKAFKLSRDRLKQVS
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEYSSYISLALYFIAMLAIGLFAYRNSTDDLAGYMLGGRQVSPHVTALSAGASDMSGWML
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHEECCCCCCCCEEE
MGLPGAMFTMGYDALWIAAGLLLGALLNYLLVAPRLRVFTEAADDALTLPDFFSKRFNKD
ECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
NGSVRMISAAVIILFFTLYTSAGLVAGGKFFESAFGLGYQTGLLLTVSVVVAYTLLGGFL
CCCCHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AVSLTDFVQGCIMFLALVLVPLVALQEFNSSTDMLAQASRSITPLADSFEHMGILAIVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LAWGLGYFGQPHIIVRFMAIRSVKDIAVARNIGMSWMLVTILGALATGLIGVAYVNKFKP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLDDGETIFILFAEILFHPLISGFLLAAILAAIMSTISSQLLVSSSSLSEDVYRALFHKE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MDQKATVTAGRIGVLAVAAVATLLALDNNASILSLVSNAWAGFGAAFGPLVLLSLFWPRM
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
THSGAVAGILCGAGTVLVWSQVPLLENGLPLSSLIYEIVPGFIVSTLAIVLVSYADQAPC
CCCCCHHHHHHCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSTQKAFKLSRDRLKQVS
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]