Click here to switch to the map view.

The map label for this gene is nemR [H]

Identifier: 119773616

GI number: 119773616

Start: 586362

End: 586952

Strand: Reverse

Name: nemR [H]

Synonym: Sama_0476

Alternate gene names: 119773616

Gene position: 586952-586362 (Counterclockwise)

Preceding gene: 119773621

Following gene: 119773615

Centisome position: 13.63

GC content: 54.82

Gene sequence:

>591_bases
ATGAATACCAAAACCCAAGACACCCGGCAACACATACTCGACATAGGCTACCAACTGGTGGTCAACAAAGGCTTTGCCGG
TGTGGGCCTGTCGGAACTGCTGAAAACCGCCGAGGTGCCCAAGGGCTCCTTCTATCACTACTTCAAGTCCAAGGAGCAGT
TTGGCGAAGCCCTTATCCAGGCATATTTCAGCGAGTACCTGCGTCAACTGAACCAGTTGCTGGAGACGGCTCCGGGTAAC
CAGTATCAAAAACTGATGCACTACTGGCAGCATTGGAGCGAGCAAAACAGCTGTCAGGGTCAGGCTGGAAAGTGCCTGGT
AGTCAAGCTTGCCGGTGAGGTATCAGACTTATCCGAGCCCATGCGCCTGGCCCTCCTCACTGGCACCAATCGCATCATAG
AGCGGGTGGCCGAGTGTCTTGAAAAGGCCCGGACTGCTGGCGAGATAAGCATCAGTAATAGCCGCCAGACAGCAGAGCAG
CTGTACAACCTGTGGATTGGCGCCAGTTTGCTGAACAAGATACGTTTGGACGATGCCAGCCTGCAGCAGGCACTGCTGAC
CACCAGACAGGTATTACAGCCAACGGCCTGA

Upstream 100 bases:

>100_bases
TACCCATCCCTTCTCCCCGAAGACGCCATTCAAAGTGAAGCGGCGATCCATTGGCACAAAAAACTAGACGACTGGTCTAA
TCGCCGTTATTATCGCCGTC

Downstream 100 bases:

>100_bases
GCCGGGTAGCCAAACACTAGAATAGCCCCAAATCCAAGCCGCTATTTCGATAGCGGATTTTTTGAAACAATCACTAGACG
ACTGGTCTAATTTAAGTAAA

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNTKTQDTRQHILDIGYQLVVNKGFAGVGLSELLKTAEVPKGSFYHYFKSKEQFGEALIQAYFSEYLRQLNQLLETAPGN
QYQKLMHYWQHWSEQNSCQGQAGKCLVVKLAGEVSDLSEPMRLALLTGTNRIIERVAECLEKARTAGEISISNSRQTAEQ
LYNLWIGASLLNKIRLDDASLQQALLTTRQVLQPTA

Sequences:

>Translated_196_residues
MNTKTQDTRQHILDIGYQLVVNKGFAGVGLSELLKTAEVPKGSFYHYFKSKEQFGEALIQAYFSEYLRQLNQLLETAPGN
QYQKLMHYWQHWSEQNSCQGQAGKCLVVKLAGEVSDLSEPMRLALLTGTNRIIERVAECLEKARTAGEISISNSRQTAEQ
LYNLWIGASLLNKIRLDDASLQQALLTTRQVLQPTA
>Mature_196_residues
MNTKTQDTRQHILDIGYQLVVNKGFAGVGLSELLKTAEVPKGSFYHYFKSKEQFGEALIQAYFSEYLRQLNQLLETAPGN
QYQKLMHYWQHWSEQNSCQGQAGKCLVVKLAGEVSDLSEPMRLALLTGTNRIIERVAECLEKARTAGEISISNSRQTAEQ
LYNLWIGASLLNKIRLDDASLQQALLTTRQVLQPTA

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=198, Percent_Identity=42.4242424242424, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22108; Mature: 22108

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKTQDTRQHILDIGYQLVVNKGFAGVGLSELLKTAEVPKGSFYHYFKSKEQFGEALIQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AYFSEYLRQLNQLLETAPGNQYQKLMHYWQHWSEQNSCQGQAGKCLVVKLAGEVSDLSEP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHH
MRLALLTGTNRIIERVAECLEKARTAGEISISNSRQTAEQLYNLWIGASLLNKIRLDDAS
HHHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHH
LQQALLTTRQVLQPTA
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTKTQDTRQHILDIGYQLVVNKGFAGVGLSELLKTAEVPKGSFYHYFKSKEQFGEALIQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AYFSEYLRQLNQLLETAPGNQYQKLMHYWQHWSEQNSCQGQAGKCLVVKLAGEVSDLSEP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHH
MRLALLTGTNRIIERVAECLEKARTAGEISISNSRQTAEQLYNLWIGASLLNKIRLDDAS
HHHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHH
LQQALLTTRQVLQPTA
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]