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The map label for this gene is uvrD [H]

Identifier: 119773559

GI number: 119773559

Start: 515732

End: 517897

Strand: Direct

Name: uvrD [H]

Synonym: Sama_0419

Alternate gene names: 119773559

Gene position: 515732-517897 (Clockwise)

Preceding gene: 119773557

Following gene: 119773560

Centisome position: 11.98

GC content: 56.83

Gene sequence:

>2166_bases
ATGGACGTTTCTTTTTTGCTGGATGGCCTGAACGATAAGCAGCGCGAAGCCGTGGCTGCACCCCAGTCCAGCATGCTGGT
GTTGGCCGGTGCCGGCAGTGGCAAGACCCGGGTGTTAACCCACCGAATCGCCTGGCTGTTGCAGGTGGAGAACCAGAGTC
CCTATTCGATATTGGCGGTAACCTTCACCAACAAGGCCGCGGCCGAAATGCGTGAGCGGGTCGAAAAAATCGTTGGTCAC
AATACCGGTCGCATGTGGATAGGTACTTTCCACGGCCTGGCCCATCGCCTGCTGCGCACCCACTGGCAGGACGCAGGTCT
GCCACAAAGTTTCCAGATCATGGACTCAGACGATCAGCAGCGTCTGCTGAAACGCATTCTCAAGAGCATGAATCTGGATG
AAAAACAGTATCCACCGCGTCTGGTTGCCGGGTATATCAACGGCAAAAAAGATCAGGGACTGCGCCCAGCCCATATAGAC
GCAGGTCCATTCCCTATGGAGCAAAAGCTGCTGGAACTGTACCGTATCTATCAGGACAGTTGTGACCGGGCTGGCTTGGT
GGACTTTGCTGAAATCCTGCTGCGTGCCCATGAGCTTTGGCTCAATAAACCCCATCTGCTGGCCCACTATCAGGACAGAT
TCCGCCATATTCTGGTGGACGAGTTTCAGGATACCAACGCCATTCAGTACGCCTGGATCCGGGTGCTGGCCGGCAACACG
GCAAATGTGATGATTGTGGGGGATGATGACCAGTCGATTTATGGCTGGCGCGGCGCCCAGATTGAAAACCTGCATCGCTT
TTTGCAGGACTTCCCCGGCGCCAAGACCATACGCCTGGAGCAAAATTACCGCTCCACCGGCAATATTCTCAAGGCCTCCA
ACGAACTTATCGCCAACAACCCCGACCGTATGGGCAAGGAGTTGTGGACCGAAGACAAGGATGGCGAGCCAATCTCCCTC
TACTGCGCCTTTAACGAGATGGACGAGGCCCGCTTTATTGTCGGCCGCATCAGCGACTGGCAGGACAAGGGCGGCATGCT
CGGTGAGTGCGCCATTCTGTACCGTTCCAACGCCCAGTCACGGGTGCTGGAAGAGGCCTTGCTCCACAAGGGAATGCCCT
ATCGTATCTATGGTGGCCTGCGCTTCTTCGAACGGCAGGAAATCAAGGATGCCATGGGCTATCTGCGCCTTATCTCCAAC
CGCGACGACGATGCTGCTTTTGAGCGTGTGGTCAATACGCCGGCCCGAGGCATAGGTGAGCGAACCCTGGATATTTTGCG
CAGTACCGCCCGCAAAGAGCAGCTGACCCTGTGGCAGACCTCGCTGCGTCTGCTCGAGGAAAAGGTGCTGTCGGGCCGCG
CTGCCAATGCGGTGAAAGGCTTTTTGGATTTGATAGTCCAGCTTCGAAGCGACACCGACGAGTTGCCGCTCTACCGCGTG
GCAGATCATGTGATTCAGCACTCAGGGCTTAAGGCCATGTACGAGGCCGAAAAGGGCGAAAACGCTCAGGCCCGGGTAGA
AAACCTTGAGGAACTGGTCACGGCCGCCCGCAGCTTTGAGATGCCGGAAGAGCTGGAAGACATGGGCGAACTCAATGCCT
TTTTGTCCCATGCGGCACTGGAGGCGGGGGAAGGTCAGGCCGATGCCCACACAGATGCTGTGCAACTGATGACGCTGCAC
TCCGCCAAGGGACTGGAATTCCCCATGGTGTTTATGGCCGGGGTGGAAGAAGGCATATTCCCCAGTAAGCTGGCTCTGGA
GGAGGGCGACCGCCTCGAAGAGGAGCGCCGCCTGTGCTATGTGGGTATGACCCGGGCAATGCAAAAGCTTTATATCACCT
ATGCCGAGTCACGCCGTATCTATGGCCGCGAAGACTATGCCAGACCCTCGCGCTTTATCCGCGAAATTCCCCCCGAGTAT
GTGGAAGAGATTCGCCTTAAAGCCCAGGTCTCGGCGCCACTGGTGGCCAATCGTTTTGCCCATGCACAGCAAACCTTTAA
TGATTCCGGCTTAAAGATTGGCGGCCGGGTACGGCACTTTAAGTTTGGTGAAGGCACTGTCACCAACTACGAGGGCAGTG
GCCCCCAGGCGAGGGTACAGGTGCAGTTTGACGATTTCGGCAGCAAATGGCTGGTGGTCAGCTACGCCAAGCTGGAATCC
CTGTGA

Upstream 100 bases:

>100_bases
CCACAGTTTCAGGCATTTGCCCAGCCCAACTCTCAGGGGCAATTCCACCCATTGCCGCAGGCTTCTGATATAATGCCGCC
TCGACTTTTGGGAGGCATGA

Downstream 100 bases:

>100_bases
GCCCAAGGGCAAAGATGCCAGAGCAAAACACAAAAGATAACAACCGCGCGATGACCCCAAATGGGGACAAGGAAAGACAA
TGAGCAAAATGGCGCAAAAA

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MDVSFLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLLQVENQSPYSILAVTFTNKAAAEMRERVEKIVGH
NTGRMWIGTFHGLAHRLLRTHWQDAGLPQSFQIMDSDDQQRLLKRILKSMNLDEKQYPPRLVAGYINGKKDQGLRPAHID
AGPFPMEQKLLELYRIYQDSCDRAGLVDFAEILLRAHELWLNKPHLLAHYQDRFRHILVDEFQDTNAIQYAWIRVLAGNT
ANVMIVGDDDQSIYGWRGAQIENLHRFLQDFPGAKTIRLEQNYRSTGNILKASNELIANNPDRMGKELWTEDKDGEPISL
YCAFNEMDEARFIVGRISDWQDKGGMLGECAILYRSNAQSRVLEEALLHKGMPYRIYGGLRFFERQEIKDAMGYLRLISN
RDDDAAFERVVNTPARGIGERTLDILRSTARKEQLTLWQTSLRLLEEKVLSGRAANAVKGFLDLIVQLRSDTDELPLYRV
ADHVIQHSGLKAMYEAEKGENAQARVENLEELVTAARSFEMPEELEDMGELNAFLSHAALEAGEGQADAHTDAVQLMTLH
SAKGLEFPMVFMAGVEEGIFPSKLALEEGDRLEEERRLCYVGMTRAMQKLYITYAESRRIYGREDYARPSRFIREIPPEY
VEEIRLKAQVSAPLVANRFAHAQQTFNDSGLKIGGRVRHFKFGEGTVTNYEGSGPQARVQVQFDDFGSKWLVVSYAKLES
L

Sequences:

>Translated_721_residues
MDVSFLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLLQVENQSPYSILAVTFTNKAAAEMRERVEKIVGH
NTGRMWIGTFHGLAHRLLRTHWQDAGLPQSFQIMDSDDQQRLLKRILKSMNLDEKQYPPRLVAGYINGKKDQGLRPAHID
AGPFPMEQKLLELYRIYQDSCDRAGLVDFAEILLRAHELWLNKPHLLAHYQDRFRHILVDEFQDTNAIQYAWIRVLAGNT
ANVMIVGDDDQSIYGWRGAQIENLHRFLQDFPGAKTIRLEQNYRSTGNILKASNELIANNPDRMGKELWTEDKDGEPISL
YCAFNEMDEARFIVGRISDWQDKGGMLGECAILYRSNAQSRVLEEALLHKGMPYRIYGGLRFFERQEIKDAMGYLRLISN
RDDDAAFERVVNTPARGIGERTLDILRSTARKEQLTLWQTSLRLLEEKVLSGRAANAVKGFLDLIVQLRSDTDELPLYRV
ADHVIQHSGLKAMYEAEKGENAQARVENLEELVTAARSFEMPEELEDMGELNAFLSHAALEAGEGQADAHTDAVQLMTLH
SAKGLEFPMVFMAGVEEGIFPSKLALEEGDRLEEERRLCYVGMTRAMQKLYITYAESRRIYGREDYARPSRFIREIPPEY
VEEIRLKAQVSAPLVANRFAHAQQTFNDSGLKIGGRVRHFKFGEGTVTNYEGSGPQARVQVQFDDFGSKWLVVSYAKLES
L
>Mature_721_residues
MDVSFLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLLQVENQSPYSILAVTFTNKAAAEMRERVEKIVGH
NTGRMWIGTFHGLAHRLLRTHWQDAGLPQSFQIMDSDDQQRLLKRILKSMNLDEKQYPPRLVAGYINGKKDQGLRPAHID
AGPFPMEQKLLELYRIYQDSCDRAGLVDFAEILLRAHELWLNKPHLLAHYQDRFRHILVDEFQDTNAIQYAWIRVLAGNT
ANVMIVGDDDQSIYGWRGAQIENLHRFLQDFPGAKTIRLEQNYRSTGNILKASNELIANNPDRMGKELWTEDKDGEPISL
YCAFNEMDEARFIVGRISDWQDKGGMLGECAILYRSNAQSRVLEEALLHKGMPYRIYGGLRFFERQEIKDAMGYLRLISN
RDDDAAFERVVNTPARGIGERTLDILRSTARKEQLTLWQTSLRLLEEKVLSGRAANAVKGFLDLIVQLRSDTDELPLYRV
ADHVIQHSGLKAMYEAEKGENAQARVENLEELVTAARSFEMPEELEDMGELNAFLSHAALEAGEGQADAHTDAVQLMTLH
SAKGLEFPMVFMAGVEEGIFPSKLALEEGDRLEEERRLCYVGMTRAMQKLYITYAESRRIYGREDYARPSRFIREIPPEY
VEEIRLKAQVSAPLVANRFAHAQQTFNDSGLKIGGRVRHFKFGEGTVTNYEGSGPQARVQVQFDDFGSKWLVVSYAKLES
L

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=721, Percent_Identity=70.0416088765603, Blast_Score=1055, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=634, Percent_Identity=38.4858044164038, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=90, Percent_Identity=45.5555555555556, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=755, Percent_Identity=27.8145695364238, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324477, Length=700, Percent_Identity=23.4285714285714, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81944; Mature: 81944

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSFLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLLQVENQSPYSILAV
CCHHHEECCCCCHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHEEEEEECCCCCEEEEEE
TFTNKAAAEMRERVEKIVGHNTGRMWIGTFHGLAHRLLRTHWQDAGLPQSFQIMDSDDQQ
EECCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHH
RLLKRILKSMNLDEKQYPPRLVAGYINGKKDQGLRPAHIDAGPFPMEQKLLELYRIYQDS
HHHHHHHHHCCCCCHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
CDRAGLVDFAEILLRAHELWLNKPHLLAHYQDRFRHILVDEFQDTNAIQYAWIRVLAGNT
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEHHEEEECCC
ANVMIVGDDDQSIYGWRGAQIENLHRFLQDFPGAKTIRLEQNYRSTGNILKASNELIANN
EEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHCCC
PDRMGKELWTEDKDGEPISLYCAFNEMDEARFIVGRISDWQDKGGMLGECAILYRSNAQS
HHHHHHHHCCCCCCCCCEEEEEEECCCCCHHEEEECCCCHHHCCCCEEEEEEEEECCCHH
RVLEEALLHKGMPYRIYGGLRFFERQEIKDAMGYLRLISNRDDDAAFERVVNTPARGIGE
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHH
RTLDILRSTARKEQLTLWQTSLRLLEEKVLSGRAANAVKGFLDLIVQLRSDTDELPLYRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
ADHVIQHSGLKAMYEAEKGENAQARVENLEELVTAARSFEMPEELEDMGELNAFLSHAAL
HHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EAGEGQADAHTDAVQLMTLHSAKGLEFPMVFMAGVEEGIFPSKLALEEGDRLEEERRLCY
HCCCCCCCCCHHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHH
VGMTRAMQKLYITYAESRRIYGREDYARPSRFIREIPPEYVEEIRLKAQVSAPLVANRFA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
HAQQTFNDSGLKIGGRVRHFKFGEGTVTNYEGSGPQARVQVQFDDFGSKWLVVSYAKLES
HHHHHCCCCCCEECCEEEEEECCCCCEECCCCCCCCEEEEEEECCCCCCEEEEEHHHHHC
L
C
>Mature Secondary Structure
MDVSFLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLLQVENQSPYSILAV
CCHHHEECCCCCHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHEEEEEECCCCCEEEEEE
TFTNKAAAEMRERVEKIVGHNTGRMWIGTFHGLAHRLLRTHWQDAGLPQSFQIMDSDDQQ
EECCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHH
RLLKRILKSMNLDEKQYPPRLVAGYINGKKDQGLRPAHIDAGPFPMEQKLLELYRIYQDS
HHHHHHHHHCCCCCHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
CDRAGLVDFAEILLRAHELWLNKPHLLAHYQDRFRHILVDEFQDTNAIQYAWIRVLAGNT
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEHHEEEECCC
ANVMIVGDDDQSIYGWRGAQIENLHRFLQDFPGAKTIRLEQNYRSTGNILKASNELIANN
EEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCHHHCCC
PDRMGKELWTEDKDGEPISLYCAFNEMDEARFIVGRISDWQDKGGMLGECAILYRSNAQS
HHHHHHHHCCCCCCCCCEEEEEEECCCCCHHEEEECCCCHHHCCCCEEEEEEEEECCCHH
RVLEEALLHKGMPYRIYGGLRFFERQEIKDAMGYLRLISNRDDDAAFERVVNTPARGIGE
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHH
RTLDILRSTARKEQLTLWQTSLRLLEEKVLSGRAANAVKGFLDLIVQLRSDTDELPLYRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
ADHVIQHSGLKAMYEAEKGENAQARVENLEELVTAARSFEMPEELEDMGELNAFLSHAAL
HHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EAGEGQADAHTDAVQLMTLHSAKGLEFPMVFMAGVEEGIFPSKLALEEGDRLEEERRLCY
HCCCCCCCCCHHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHH
VGMTRAMQKLYITYAESRRIYGREDYARPSRFIREIPPEYVEEIRLKAQVSAPLVANRFA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
HAQQTFNDSGLKIGGRVRHFKFGEGTVTNYEGSGPQARVQVQFDDFGSKWLVVSYAKLES
HHHHHCCCCCCEECCEEEEEECCCCCEECCCCCCCCEEEEEEECCCCCCEEEEEHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]