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The map label for this gene is emrB [H]

Identifier: 119773543

GI number: 119773543

Start: 500902

End: 502491

Strand: Direct

Name: emrB [H]

Synonym: Sama_0403

Alternate gene names: 119773543

Gene position: 500902-502491 (Clockwise)

Preceding gene: 119773542

Following gene: 119773544

Centisome position: 11.63

GC content: 55.91

Gene sequence:

>1590_bases
ATGAGTGCGAGTATCGCGTTGCCGCAAACCGATGTGGATTACGGTGTAGGCAGTCGCCGCGCCTGGATTGCCGTGATGGG
CGGCCTTATCGGGGCATTTATGGCGATTCTGGACATCCAGATCACCAATGCCTCCATGAAGGAGATCCAGGGCAGCCTGG
GGGCGACTCTTGAAGAGGGCTCTTGGATTTCAACCGCTTATCTGGTGGCTGAAATGATAGCCATTCCCCTGTCGGGATGG
CTCAGTACCGGTTTGTCGGTGCGCCGTTACCTGCTGTGGAGTACTACCGCCTTTATTCTCAGCTCGCTGCTTTGTTCCAT
GGCATGGGATTTAAACTCCCTGATTGCATTTCGTGCCCTGCAGGGATTCTTTGGCGGCGCCCTCATTCCGCTGGCGTTTC
GTCTGATTGTGGAATGCCTGCCTTTTGAAAAGCGTGCCATGGGGATGGCGCTCTTTGGGGTCACGGCAACCTTTGCGCCT
TCCATCGGGCCTACTCTGGGTGGCTGGCTAACCGAGCATTTCAGCTGGCATTTTCTGTTCTATATCAATGTGCCACCCGG
ACTTCTTGTACTGTGGATGCTGGCCTATGGCCTGCAAAAAGGCCCCATCGATTGGGGCAAACTTGGCAAGGTGAGCTGGG
TTGGTATCGCCACCATGGCGCTCGGCCTTGGCTGCCTTGAAGTGGTGCTGGAGGAGGGGAACCGAAAAGACTGGCTCGGT
TCCGACGTTATCCGTGATTTGGCCATCATAGCCGCCATCAACCTGGTGCTCTTTGTCTATTTGCAGCTGAGGAGCCGCAA
CCCGCTGGTTAACCTGCGTCTCTTAGCCCACAGGGACTTTGCGTTGTCGTCGTTGTCGTATTTTTTGCTGGGACTGGGGC
TTTTTGGCGCGATTTACCTTATTCCGCTTTACCTGTCGCAGATCCATAATTATTCGGCGCTGGAAATTGGCAAGGTCATC
ATGTGGATGGGGTTCCCCCAGCTTTTGGTGCTGCCTCTGGTACCTAAGTTGACCCAAAAGCTGGATCCCCGCTATCTGGC
GGCCTGGGGCTTTTTGTTGTTTGCTTTGAGTTACTATCTCAATGCCCATATGACGGCCCAGTACGCCGGTGAGCAGATGA
TTCTGGCGCAAATCGTCAGGGCGCTCGGGCAGCCATTCGTGCTGGTACCCATCGGTATGATAGCGACCATGCATTTGCGT
CAGGAAGATACCCCAAGCGCGTCTACGCTACTGAATCTTATCCGAAACCTCGGTGGTGCCTGTGGCATTGCGCTGGTGGC
GACCATGCTGGATAACCGTTTCCGCCAGCATCTGGCGTACTTGAAAGAGTCGCTGCCTGCGGTCAGTCAGCAGGCGCATA
CCTTCCTGCAACAGCAGGCAAGTAGCCTGTTTGCAGCCGGGGCCGATCCTATGGCGGCCGATTCGATGGCCCGGAAGATG
CTGTCTGACACCCTGGTGCGGGAAGCTTACATGCAGGCCTACAACGATGTGTTCTTTGCCATCGGGGGCTTGCTGTTGGT
TGCCGTCGTGGCTGTGTTGCTCATCAGACCTTCTTCTCAGGCTGGCAATAGCGGCGCAGCCGGACACTGA

Upstream 100 bases:

>100_bases
GGAGAGCAAAAGGTGGTACCGGGGCTCAGCGCCGTAGTGTCCGTGGATACGGCCGCAACGCCCGCGCTGACAGTGACCGC
CGGTCGCTGAGGGGACGGGC

Downstream 100 bases:

>100_bases
AGCCGGTATTTGTGTTGGTGGAAGTGGCTGTATGGCTAACTGTCCACGGCGGTGCTGCAAGGGGCTGATAGCGATGATAG
ACTGACCGCGGTCAATCATC

Product: drug resistance transporter, EmrB/QacA family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSASIALPQTDVDYGVGSRRAWIAVMGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYLVAEMIAIPLSGW
LSTGLSVRRYLLWSTTAFILSSLLCSMAWDLNSLIAFRALQGFFGGALIPLAFRLIVECLPFEKRAMGMALFGVTATFAP
SIGPTLGGWLTEHFSWHFLFYINVPPGLLVLWMLAYGLQKGPIDWGKLGKVSWVGIATMALGLGCLEVVLEEGNRKDWLG
SDVIRDLAIIAAINLVLFVYLQLRSRNPLVNLRLLAHRDFALSSLSYFLLGLGLFGAIYLIPLYLSQIHNYSALEIGKVI
MWMGFPQLLVLPLVPKLTQKLDPRYLAAWGFLLFALSYYLNAHMTAQYAGEQMILAQIVRALGQPFVLVPIGMIATMHLR
QEDTPSASTLLNLIRNLGGACGIALVATMLDNRFRQHLAYLKESLPAVSQQAHTFLQQQASSLFAAGADPMAADSMARKM
LSDTLVREAYMQAYNDVFFAIGGLLLVAVVAVLLIRPSSQAGNSGAAGH

Sequences:

>Translated_529_residues
MSASIALPQTDVDYGVGSRRAWIAVMGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYLVAEMIAIPLSGW
LSTGLSVRRYLLWSTTAFILSSLLCSMAWDLNSLIAFRALQGFFGGALIPLAFRLIVECLPFEKRAMGMALFGVTATFAP
SIGPTLGGWLTEHFSWHFLFYINVPPGLLVLWMLAYGLQKGPIDWGKLGKVSWVGIATMALGLGCLEVVLEEGNRKDWLG
SDVIRDLAIIAAINLVLFVYLQLRSRNPLVNLRLLAHRDFALSSLSYFLLGLGLFGAIYLIPLYLSQIHNYSALEIGKVI
MWMGFPQLLVLPLVPKLTQKLDPRYLAAWGFLLFALSYYLNAHMTAQYAGEQMILAQIVRALGQPFVLVPIGMIATMHLR
QEDTPSASTLLNLIRNLGGACGIALVATMLDNRFRQHLAYLKESLPAVSQQAHTFLQQQASSLFAAGADPMAADSMARKM
LSDTLVREAYMQAYNDVFFAIGGLLLVAVVAVLLIRPSSQAGNSGAAGH
>Mature_528_residues
SASIALPQTDVDYGVGSRRAWIAVMGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYLVAEMIAIPLSGWL
STGLSVRRYLLWSTTAFILSSLLCSMAWDLNSLIAFRALQGFFGGALIPLAFRLIVECLPFEKRAMGMALFGVTATFAPS
IGPTLGGWLTEHFSWHFLFYINVPPGLLVLWMLAYGLQKGPIDWGKLGKVSWVGIATMALGLGCLEVVLEEGNRKDWLGS
DVIRDLAIIAAINLVLFVYLQLRSRNPLVNLRLLAHRDFALSSLSYFLLGLGLFGAIYLIPLYLSQIHNYSALEIGKVIM
WMGFPQLLVLPLVPKLTQKLDPRYLAAWGFLLFALSYYLNAHMTAQYAGEQMILAQIVRALGQPFVLVPIGMIATMHLRQ
EDTPSASTLLNLIRNLGGACGIALVATMLDNRFRQHLAYLKESLPAVSQQAHTFLQQQASSLFAAGADPMAADSMARKML
SDTLVREAYMQAYNDVFFAIGGLLLVAVVAVLLIRPSSQAGNSGAAGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=492, Percent_Identity=30.6910569105691, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1788710, Length=427, Percent_Identity=33.9578454332553, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI87081983, Length=430, Percent_Identity=27.2093023255814, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1790195, Length=447, Percent_Identity=24.8322147651007, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788392, Length=425, Percent_Identity=25.6470588235294, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=140, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57642; Mature: 57511

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASIALPQTDVDYGVGSRRAWIAVMGGLIGAFMAILDIQITNASMKEIQGSLGATLEEG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
SWISTAYLVAEMIAIPLSGWLSTGLSVRRYLLWSTTAFILSSLLCSMAWDLNSLIAFRAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGFFGGALIPLAFRLIVECLPFEKRAMGMALFGVTATFAPSIGPTLGGWLTEHFSWHFLF
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEE
YINVPPGLLVLWMLAYGLQKGPIDWGKLGKVSWVGIATMALGLGCLEVVLEEGNRKDWLG
EEECCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SDVIRDLAIIAAINLVLFVYLQLRSRNPLVNLRLLAHRDFALSSLSYFLLGLGLFGAIYL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHH
IPLYLSQIHNYSALEIGKVIMWMGFPQLLVLPLVPKLTQKLDPRYLAAWGFLLFALSYYL
HHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHH
NAHMTAQYAGEQMILAQIVRALGQPFVLVPIGMIATMHLRQEDTPSASTLLNLIRNLGGA
HHHHHHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCH
CGIALVATMLDNRFRQHLAYLKESLPAVSQQAHTFLQQQASSLFAAGADPMAADSMARKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LSDTLVREAYMQAYNDVFFAIGGLLLVAVVAVLLIRPSSQAGNSGAAGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SASIALPQTDVDYGVGSRRAWIAVMGGLIGAFMAILDIQITNASMKEIQGSLGATLEEG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
SWISTAYLVAEMIAIPLSGWLSTGLSVRRYLLWSTTAFILSSLLCSMAWDLNSLIAFRAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGFFGGALIPLAFRLIVECLPFEKRAMGMALFGVTATFAPSIGPTLGGWLTEHFSWHFLF
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEE
YINVPPGLLVLWMLAYGLQKGPIDWGKLGKVSWVGIATMALGLGCLEVVLEEGNRKDWLG
EEECCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SDVIRDLAIIAAINLVLFVYLQLRSRNPLVNLRLLAHRDFALSSLSYFLLGLGLFGAIYL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHH
IPLYLSQIHNYSALEIGKVIMWMGFPQLLVLPLVPKLTQKLDPRYLAAWGFLLFALSYYL
HHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHH
NAHMTAQYAGEQMILAQIVRALGQPFVLVPIGMIATMHLRQEDTPSASTLLNLIRNLGGA
HHHHHHHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCH
CGIALVATMLDNRFRQHLAYLKESLPAVSQQAHTFLQQQASSLFAAGADPMAADSMARKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LSDTLVREAYMQAYNDVFFAIGGLLLVAVVAVLLIRPSSQAGNSGAAGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]