Click here to switch to the map view.

The map label for this gene is aceF [H]

Identifier: 119773517

GI number: 119773517

Start: 456531

End: 458459

Strand: Direct

Name: aceF [H]

Synonym: Sama_0376

Alternate gene names: 119773517

Gene position: 456531-458459 (Clockwise)

Preceding gene: 119773516

Following gene: 119773518

Centisome position: 10.6

GC content: 58.11

Gene sequence:

>1929_bases
ATGGCAATCGAAATTCATGTACCCGATATCGGCACTGATGAAGTCGAAGTGACCGAGATCCTGGTCAAGGTGGGTGATTC
CATCACTGAGGATCAGTCGCTGATCACCGTGGAAGGCGACAAGTCTTCCATGGAAGTACCGGCTCCCAAGGCTGGTGTGG
TCAAGGAAATCAAAATTGCGGTGGGCGACAAGGTCGCCACCGGCTCACTGATCATGATGTTTGAAGGTGAGCAAGCAGCT
GCTGCTCCGGCGGCCCAGGCCGCTGCGCCAGTGCCGGTTGCTGCAGCCCCAGCTGCTACAGCGGTAGCCGCGCTGAAAGA
CGTATTCGTGCCTGACATCGGCGGTGACGAAGTTGAAGTCACTGAAATTCTGGTGAAAGTAGGCGACAGCATCACTGATG
AGCAGCCACTGATCACAGTAGAAGGCGACAAGGCTTCTATGGAAGTGCCTGCGCCGTTCGCCGGTGTGCTCAAAGAAATC
AAGGTCGCCACCGGTGATAAGGTAGCCACTGGCTCCCTTATCATGGTGTTCGAGACTCAGGCTGCATCCGCTGCTCCTGT
GGCCGCAGCTGCTCCTGTGGCCGCAGCTGCTGCGCCAGCGCCGGTTGCCGCCGCGCCAGTTGCCGGCGGTGACAAAGACA
TTCACGTGCCGGACATCGGCGGCGATGAAGTGGAAGTGACCGAAGTGCTGGTGAAGGTAGGCGACACAGTTGCCGCCGAC
CAGTCGCTGCTGACAGTGGAAGGCGACAAAGCCTCTATGGAAGTACCTGCGCCGGTTGCCGGTGTGGTTAAGGCCATCAA
GGTGGCTGCCGGTGACAAGGTGTCTACCGGTTCACTGATCATGGTGTTCGAAGTACAAGGTGCTGCTCCTGCTGCCGCGC
CAGCTGCACCTGTGGCTCCGGCCGCTCCTCAAGCTGCGGCTCCGGCTGCCAATGCCCAGGCCCAGGCTCCGTCTCAGGGG
CAAAGTAAGACTGGTGAGTTCGTGGAAAACCACGAGTACGCCCACGCGTCACCGGTTATCCGTCGTCTGGCCCGTGAGTT
TGGCGTTAACCTTGCCAAGGTCAAGGGTACAGGTCGTAAGGGCCGAGTGGTACGTGAAGACGTGCAGTCTTACATCAAAG
AAGCACTCAAGCGCCTCGAGTCAGGCTCTGCTACCGCCGCCGCGGGCGCTGCCTCCGGCGCTGGCCTGAACCTGCTGCCA
TGGCCGAAGGTTGACTTCAGCAAATTTGGTGAAGTTGAGACTCAGCCGTTGTCACGCATCAAGAAGATCAGTGGTGCCAA
CCTGCACCGTAACTGGGTGATGATCCCCCATGTGACTCAGTTTGATGAGGCCGACATCACCGAGCTTGAGGACTTCCGCA
AGGCGCAAAACGCCGAAGAAGCCAAGCGCGATACCGGTATGAAGATCACCCCGCTGGTGTTCATCATGAAGGCGGTGGCC
AAGTCGCTGGAAGCCTTCCCATCATTCAACGCGTCTCTGTCTGAAGATGGCGAGAGCCTTATTCTGAAGAAGTACATCAA
CATCGGCATTGCGGTGGATACCCCCAATGGTCTGGTGGTGCCTGTGGTACGTGATGTGAACAAGAAAGGCATCTATGAGC
TGTCCCGCGAGCTGGCTGACATCTCCAAGAAGGCCCGCGCCGGTAAGCTTACTGCCTCTGACATGCAGGGCGGCTGCTTT
ACCATCTCCAGCCTCGGTGGCCTCGGTGGTACCTCGTTCACCCCAATCGTGAATGCCCCTGAAGTGGGTATTCTGGGTGT
GTCCAAGTCTGAGCTCAAGCCCAAGTGGAACGGCAAGGAATTTGTGCCTCGCCTGATGTTGCCACTGTCGCTGTCGTACG
ACCACCGGGTGATCGACGGCGCCGAAGGTGCCAAGTTCACCACGTATCTGAGCGGTGTACTGTCAGACATACGCAAACTC
GTTCTGTAA

Upstream 100 bases:

>100_bases
AGCGCGGTGACATCGAGAAATCTGTGGTGGTTGCTGCCATCAAGAAGTTCGATATCGACACCGAGAAGATGAACCCACTT
TACGCGTAAGAGACGAGACA

Downstream 100 bases:

>100_bases
CCGTAAAGGCTGTCCTGAATAGGACGGCCTTTTTATTGTGATCCGTGTCGAACGCGGGTTAAAATGCGGCCACCTTACAA
GCGGGCCGATAGTTGGTTTA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MAIEIHVPDIGTDEVEVTEILVKVGDSITEDQSLITVEGDKSSMEVPAPKAGVVKEIKIAVGDKVATGSLIMMFEGEQAA
AAPAAQAAAPVPVAAAPAATAVAALKDVFVPDIGGDEVEVTEILVKVGDSITDEQPLITVEGDKASMEVPAPFAGVLKEI
KVATGDKVATGSLIMVFETQAASAAPVAAAAPVAAAAAPAPVAAAPVAGGDKDIHVPDIGGDEVEVTEVLVKVGDTVAAD
QSLLTVEGDKASMEVPAPVAGVVKAIKVAAGDKVSTGSLIMVFEVQGAAPAAAPAAPVAPAAPQAAAPAANAQAQAPSQG
QSKTGEFVENHEYAHASPVIRRLAREFGVNLAKVKGTGRKGRVVREDVQSYIKEALKRLESGSATAAAGAASGAGLNLLP
WPKVDFSKFGEVETQPLSRIKKISGANLHRNWVMIPHVTQFDEADITELEDFRKAQNAEEAKRDTGMKITPLVFIMKAVA
KSLEAFPSFNASLSEDGESLILKKYINIGIAVDTPNGLVVPVVRDVNKKGIYELSRELADISKKARAGKLTASDMQGGCF
TISSLGGLGGTSFTPIVNAPEVGILGVSKSELKPKWNGKEFVPRLMLPLSLSYDHRVIDGAEGAKFTTYLSGVLSDIRKL
VL

Sequences:

>Translated_642_residues
MAIEIHVPDIGTDEVEVTEILVKVGDSITEDQSLITVEGDKSSMEVPAPKAGVVKEIKIAVGDKVATGSLIMMFEGEQAA
AAPAAQAAAPVPVAAAPAATAVAALKDVFVPDIGGDEVEVTEILVKVGDSITDEQPLITVEGDKASMEVPAPFAGVLKEI
KVATGDKVATGSLIMVFETQAASAAPVAAAAPVAAAAAPAPVAAAPVAGGDKDIHVPDIGGDEVEVTEVLVKVGDTVAAD
QSLLTVEGDKASMEVPAPVAGVVKAIKVAAGDKVSTGSLIMVFEVQGAAPAAAPAAPVAPAAPQAAAPAANAQAQAPSQG
QSKTGEFVENHEYAHASPVIRRLAREFGVNLAKVKGTGRKGRVVREDVQSYIKEALKRLESGSATAAAGAASGAGLNLLP
WPKVDFSKFGEVETQPLSRIKKISGANLHRNWVMIPHVTQFDEADITELEDFRKAQNAEEAKRDTGMKITPLVFIMKAVA
KSLEAFPSFNASLSEDGESLILKKYINIGIAVDTPNGLVVPVVRDVNKKGIYELSRELADISKKARAGKLTASDMQGGCF
TISSLGGLGGTSFTPIVNAPEVGILGVSKSELKPKWNGKEFVPRLMLPLSLSYDHRVIDGAEGAKFTTYLSGVLSDIRKL
VL
>Mature_641_residues
AIEIHVPDIGTDEVEVTEILVKVGDSITEDQSLITVEGDKSSMEVPAPKAGVVKEIKIAVGDKVATGSLIMMFEGEQAAA
APAAQAAAPVPVAAAPAATAVAALKDVFVPDIGGDEVEVTEILVKVGDSITDEQPLITVEGDKASMEVPAPFAGVLKEIK
VATGDKVATGSLIMVFETQAASAAPVAAAAPVAAAAAPAPVAAAPVAGGDKDIHVPDIGGDEVEVTEVLVKVGDTVAADQ
SLLTVEGDKASMEVPAPVAGVVKAIKVAAGDKVSTGSLIMVFEVQGAAPAAAPAAPVAPAAPQAAAPAANAQAQAPSQGQ
SKTGEFVENHEYAHASPVIRRLAREFGVNLAKVKGTGRKGRVVREDVQSYIKEALKRLESGSATAAAGAASGAGLNLLPW
PKVDFSKFGEVETQPLSRIKKISGANLHRNWVMIPHVTQFDEADITELEDFRKAQNAEEAKRDTGMKITPLVFIMKAVAK
SLEAFPSFNASLSEDGESLILKKYINIGIAVDTPNGLVVPVVRDVNKKGIYELSRELADISKKARAGKLTASDMQGGCFT
ISSLGGLGGTSFTPIVNAPEVGILGVSKSELKPKWNGKEFVPRLMLPLSLSYDHRVIDGAEGAKFTTYLSGVLSDIRKLV
L

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=497, Percent_Identity=28.169014084507, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI31711992, Length=308, Percent_Identity=32.7922077922078, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=32.7510917030568, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI203098816, Length=482, Percent_Identity=26.7634854771784, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI203098753, Length=443, Percent_Identity=26.410835214447, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI260898739, Length=142, Percent_Identity=35.2112676056338, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786305, Length=644, Percent_Identity=70.1863354037267, Blast_Score=803, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=32.0276497695853, Blast_Score=185, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=29.5508274231678, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=28.4403669724771, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=208, Percent_Identity=36.5384615384615, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=313, Percent_Identity=28.1150159744409, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=238, Percent_Identity=33.6134453781513, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=436, Percent_Identity=27.9816513761468, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=30.5747126436782, Blast_Score=170, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24645909, Length=210, Percent_Identity=36.1904761904762, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=28.5106382978723, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=28.5106382978723, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 66377; Mature: 66245

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIHVPDIGTDEVEVTEILVKVGDSITEDQSLITVEGDKSSMEVPAPKAGVVKEIKIA
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKVATGSLIMMFEGEQAAAAPAAQAAAPVPVAAAPAATAVAALKDVFVPDIGGDEVEV
ECCEECCCCEEEEECCCCHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHH
TEILVKVGDSITDEQPLITVEGDKASMEVPAPFAGVLKEIKVATGDKVATGSLIMVFETQ
HHHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHCCCCCEECCCEEEEEECC
AASAAPVAAAAPVAAAAAPAPVAAAPVAGGDKDIHVPDIGGDEVEVTEVLVKVGDTVAAD
CCCCCCCHHHCCHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCHHCC
QSLLTVEGDKASMEVPAPVAGVVKAIKVAAGDKVSTGSLIMVFEVQGAAPAAAPAAPVAP
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
AAPQAAAPAANAQAQAPSQGQSKTGEFVENHEYAHASPVIRRLAREFGVNLAKVKGTGRK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCC
GRVVREDVQSYIKEALKRLESGSATAAAGAASGAGLNLLPWPKVDFSKFGEVETQPLSRI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHH
KKISGANLHRNWVMIPHVTQFDEADITELEDFRKAQNAEEAKRDTGMKITPLVFIMKAVA
HHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEEHHHHHHHHHHH
KSLEAFPSFNASLSEDGESLILKKYINIGIAVDTPNGLVVPVVRDVNKKGIYELSRELAD
HHHHHCCCCCCCCCCCCHHHHHHHHCEEEEEEECCCCEEEEHHHCCCCCHHHHHHHHHHH
ISKKARAGKLTASDMQGGCFTISSLGGLGGTSFTPIVNAPEVGILGVSKSELKPKWNGKE
HHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCCHH
FVPRLMLPLSLSYDHRVIDGAEGAKFTTYLSGVLSDIRKLVL
HHHHHHCCEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AIEIHVPDIGTDEVEVTEILVKVGDSITEDQSLITVEGDKSSMEVPAPKAGVVKEIKIA
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKVATGSLIMMFEGEQAAAAPAAQAAAPVPVAAAPAATAVAALKDVFVPDIGGDEVEV
ECCEECCCCEEEEECCCCHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHH
TEILVKVGDSITDEQPLITVEGDKASMEVPAPFAGVLKEIKVATGDKVATGSLIMVFETQ
HHHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHCCCCCEECCCEEEEEECC
AASAAPVAAAAPVAAAAAPAPVAAAPVAGGDKDIHVPDIGGDEVEVTEVLVKVGDTVAAD
CCCCCCCHHHCCHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCHHCC
QSLLTVEGDKASMEVPAPVAGVVKAIKVAAGDKVSTGSLIMVFEVQGAAPAAAPAAPVAP
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
AAPQAAAPAANAQAQAPSQGQSKTGEFVENHEYAHASPVIRRLAREFGVNLAKVKGTGRK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCC
GRVVREDVQSYIKEALKRLESGSATAAAGAASGAGLNLLPWPKVDFSKFGEVETQPLSRI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHH
KKISGANLHRNWVMIPHVTQFDEADITELEDFRKAQNAEEAKRDTGMKITPLVFIMKAVA
HHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEEHHHHHHHHHHH
KSLEAFPSFNASLSEDGESLILKKYINIGIAVDTPNGLVVPVVRDVNKKGIYELSRELAD
HHHHHCCCCCCCCCCCCHHHHHHHHCEEEEEEECCCCEEEEHHHCCCCCHHHHHHHHHHH
ISKKARAGKLTASDMQGGCFTISSLGGLGGTSFTPIVNAPEVGILGVSKSELKPKWNGKE
HHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCCHH
FVPRLMLPLSLSYDHRVIDGAEGAKFTTYLSGVLSDIRKLVL
HHHHHHCCEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]