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The map label for this gene is lpdA [H]

Identifier: 119773518

GI number: 119773518

Start: 458630

End: 460060

Strand: Direct

Name: lpdA [H]

Synonym: Sama_0377

Alternate gene names: 119773518

Gene position: 458630-460060 (Clockwise)

Preceding gene: 119773517

Following gene: 119773519

Centisome position: 10.65

GC content: 54.72

Gene sequence:

>1431_bases
ATGAGTAATCAAATCAAAACTCAGGTAGTAGTGTTGGGCGCGGGCCCTGCGGGTTATTCCGCCGCTTTCCGTGCTGCCGA
CCTTGGTCTGGAAACCGTAATCATCGAGCGCTTCAGCACCCTGGGTGGCGTGTGTCTGAACGTGGGTTGTATCCCATCAA
AGGCCCTGCTGCACATTGCCAAAGTGATTGAAGAAGCCAAAACTGCTGCCGAGCACGGCGTAGTATTCGGCGAGCCAAGC
ATCGATATCGACAAGCTGCGCGGCTACAAAGAGAAAGTGATCGGCCAACTGACCGGTGGTCTGGGTGGCATGGCCAAGAT
GCGTAAAGTGCAGGTGGTGAACGGTCTGGGTAAATTCACCGGCCCTAACAGCATCGAAGTAACAGGTGCCGATGGCGCCG
CTACCGTAATCAACTTCGACAACGCCATCATTGCTGCCGGTTCACGTCCAATCCAGCTGCCATTCATTCCTCATGAAGAC
CCACGTATTTGGGACTCTACCGATGCGCTGGAACTGAAAGAAGTACCCAAGAAGCTGCTGGTGATGGGCGGTGGTATTAT
CGGTCTGGAAATGGGCACTGTTTACCATGCGCTGGGCAGTGACATCGACGTGGTTGAAATGTTCGACCAGGTTATCCCAG
CCGCAGACAAAGACGTGGTGAAGGTTTACACCCGTAAGATCAGCAAGAAATTCAACCTGATGCTCGAAACCAAAGTCACC
GCGGTTGAAGCCAAAGAAGACGGTATCTACGTCTCTATGGAAGGCAAGAAGGCCCCAACTGAGCCTGTGCGTTACGACGC
CGTGCTGGTGGCCATCGGCCGTACTCCTAACGGCAAGCTGATCGATGCCGACAAGGCGGGCATCAATGTTGATGAGCGTG
GCTTTATCAAGGTAGACAAGCAGCTGCGCACCAACGTGCCACACATCTTCGCCGTGGGCGACATCGTGGGTCAGCCAATG
CTGGCGCACAAAGGTGTGCATGAAGGCCACGTTGCTGCCGAGGTGATCTCTGGCCTGAAGCACTTCTTCGATCCTAAGGT
GATCCCATCTATCGCTTACACCGACCCTGAAGTGGCCTGGGTAGGTCTGACCGAGAAAGAAGCCAAAGAGAAAGGCATCG
CTTACGAAACTGCGACCTTCCCATGGGCTGCTTCCGGCCGCGCCATCGCCTCTGATGCCAGCGACGGTATGACCAAGCTG
ATTTTCGACAAAGAAACTCACCGCGTCATTGGTGGTGCCATCGTGGGTGTGAACGGCGGCGAACTGCTGGGCGAAATCGG
TCTGGCAATCGAAATGGGCTGTGATGCTGAAGATATCGCGCTGACCATCCACGCTCACCCAACTCTGCACGAGTCTGTGG
GTCTGGCGGCTGAGGTTTACGAAGGTTCTATCACTGACCTGCCAAACCCAAAGGCGAAAAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
CACCTTACAAGCGGGCCGATAGTTGGTTTAAAAAGTCGCCTCCCTCAAATGCTTGAGCAACTTTTTAAACGACGGAATGA
AGAAAAATTCAGAGGAAAAC

Downstream 100 bases:

>100_bases
TTTTCTGAAAAGCCAAAAAAAGACGCCCAAGGGGCGTCTTTTTTTATGGCTCTATTTGTGAATGGTGTAAAATTTGTGTA
ATTATCTCGTGGTGTTAGGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSNQIKTQVVVLGAGPAGYSAAFRAADLGLETVIIERFSTLGGVCLNVGCIPSKALLHIAKVIEEAKTAAEHGVVFGEPS
IDIDKLRGYKEKVIGQLTGGLGGMAKMRKVQVVNGLGKFTGPNSIEVTGADGAATVINFDNAIIAAGSRPIQLPFIPHED
PRIWDSTDALELKEVPKKLLVMGGGIIGLEMGTVYHALGSDIDVVEMFDQVIPAADKDVVKVYTRKISKKFNLMLETKVT
AVEAKEDGIYVSMEGKKAPTEPVRYDAVLVAIGRTPNGKLIDADKAGINVDERGFIKVDKQLRTNVPHIFAVGDIVGQPM
LAHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAWVGLTEKEAKEKGIAYETATFPWAASGRAIASDASDGMTKL
IFDKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVYEGSITDLPNPKAKKKK

Sequences:

>Translated_476_residues
MSNQIKTQVVVLGAGPAGYSAAFRAADLGLETVIIERFSTLGGVCLNVGCIPSKALLHIAKVIEEAKTAAEHGVVFGEPS
IDIDKLRGYKEKVIGQLTGGLGGMAKMRKVQVVNGLGKFTGPNSIEVTGADGAATVINFDNAIIAAGSRPIQLPFIPHED
PRIWDSTDALELKEVPKKLLVMGGGIIGLEMGTVYHALGSDIDVVEMFDQVIPAADKDVVKVYTRKISKKFNLMLETKVT
AVEAKEDGIYVSMEGKKAPTEPVRYDAVLVAIGRTPNGKLIDADKAGINVDERGFIKVDKQLRTNVPHIFAVGDIVGQPM
LAHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAWVGLTEKEAKEKGIAYETATFPWAASGRAIASDASDGMTKL
IFDKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVYEGSITDLPNPKAKKKK
>Mature_475_residues
SNQIKTQVVVLGAGPAGYSAAFRAADLGLETVIIERFSTLGGVCLNVGCIPSKALLHIAKVIEEAKTAAEHGVVFGEPSI
DIDKLRGYKEKVIGQLTGGLGGMAKMRKVQVVNGLGKFTGPNSIEVTGADGAATVINFDNAIIAAGSRPIQLPFIPHEDP
RIWDSTDALELKEVPKKLLVMGGGIIGLEMGTVYHALGSDIDVVEMFDQVIPAADKDVVKVYTRKISKKFNLMLETKVTA
VEAKEDGIYVSMEGKKAPTEPVRYDAVLVAIGRTPNGKLIDADKAGINVDERGFIKVDKQLRTNVPHIFAVGDIVGQPML
AHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAWVGLTEKEAKEKGIAYETATFPWAASGRAIASDASDGMTKLI
FDKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVYEGSITDLPNPKAKKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=456, Percent_Identity=44.078947368421, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI50301238, Length=454, Percent_Identity=28.1938325991189, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI148277065, Length=430, Percent_Identity=27.906976744186, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI33519430, Length=430, Percent_Identity=27.906976744186, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI33519428, Length=430, Percent_Identity=27.906976744186, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI33519426, Length=430, Percent_Identity=27.906976744186, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI148277071, Length=430, Percent_Identity=28.1395348837209, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=28.7037037037037, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI22035672, Length=462, Percent_Identity=27.2727272727273, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI291045268, Length=426, Percent_Identity=27.2300469483568, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=88.2105263157895, Blast_Score=858, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=27.0788912579957, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=28.2894736842105, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789915, Length=445, Percent_Identity=27.4157303370787, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=39.8678414096916, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=28.0590717299578, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=437, Percent_Identity=27.0022883295195, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=437, Percent_Identity=27.0022883295195, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=448, Percent_Identity=26.1160714285714, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.2280701754386, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=26.2820512820513, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=454, Percent_Identity=27.5330396475771, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.0437636761488, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24640549, Length=466, Percent_Identity=29.8283261802575, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640553, Length=466, Percent_Identity=29.8283261802575, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640551, Length=466, Percent_Identity=29.8283261802575, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17737741, Length=485, Percent_Identity=26.1855670103093, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50548; Mature: 50417

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQIKTQVVVLGAGPAGYSAAFRAADLGLETVIIERFSTLGGVCLNVGCIPSKALLHIA
CCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKTAAEHGVVFGEPSIDIDKLRGYKEKVIGQLTGGLGGMAKMRKVQVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
GPNSIEVTGADGAATVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPKKLL
CCCEEEEECCCCCEEEEECCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
VMGGGIIGLEMGTVYHALGSDIDVVEMFDQVIPAADKDVVKVYTRKISKKFNLMLETKVT
HHCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAKEDGIYVSMEGKKAPTEPVRYDAVLVAIGRTPNGKLIDADKAGINVDERGFIKVDK
EEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCCCCEEEECH
QLRTNVPHIFAVGDIVGQPMLAHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEKGIAYETATFPWAASGRAIASDASDGMTKLIFDKETHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCCEEEEECCCHHEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVYEGSITDLPNPKAKKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNQIKTQVVVLGAGPAGYSAAFRAADLGLETVIIERFSTLGGVCLNVGCIPSKALLHIA
CCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKTAAEHGVVFGEPSIDIDKLRGYKEKVIGQLTGGLGGMAKMRKVQVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
GPNSIEVTGADGAATVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPKKLL
CCCEEEEECCCCCEEEEECCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
VMGGGIIGLEMGTVYHALGSDIDVVEMFDQVIPAADKDVVKVYTRKISKKFNLMLETKVT
HHCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAKEDGIYVSMEGKKAPTEPVRYDAVLVAIGRTPNGKLIDADKAGINVDERGFIKVDK
EEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCCCCEEEECH
QLRTNVPHIFAVGDIVGQPMLAHKGVHEGHVAAEVISGLKHFFDPKVIPSIAYTDPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEKGIAYETATFPWAASGRAIASDASDGMTKLIFDKETHRVIGGAIVGVNGG
EECCHHHHHHCCCEEEECCCCCCCCCCEEECCCCCCCEEEEECCCHHEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVYEGSITDLPNPKAKKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]