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The map label for this gene is aceE [H]

Identifier: 119773516

GI number: 119773516

Start: 453823

End: 456519

Strand: Direct

Name: aceE [H]

Synonym: Sama_0375

Alternate gene names: 119773516

Gene position: 453823-456519 (Clockwise)

Preceding gene: 119773515

Following gene: 119773517

Centisome position: 10.54

GC content: 55.14

Gene sequence:

>2697_bases
ATGTCTGAACCTATGCAAATGCTACAAGACTTAGATCCCATCGAAACCCAGGAGTGGCTGGCGGCCCTCGAGTCCGTTGT
TCGCGAAGAAGGCGTCGAACGTGCCCAATTCCTGCTGGAGCAAGTGTTGGAGCAGGCCCGTCTTGATGGTGTGGACATGC
CAACCGGCATCACCACCAACTATGTGAACACCATCCCCTTGACTCAGGAACCGGCTTACCCTGGTGATATCACCATTGAA
CGCCGCATCCGTTCCATCATTCGCTGGAACGCCATCATGATAGTACTGCGCGCCTCCAAGAAAGATCTGGAGCTGGGTGG
TCACATGGCGTCTTTCCAGTCGGCGGCTGCTTTCTACGAAGTGTGTTTCAACCACTTTTTCCGCGCCCCCAACCACAAAG
ATGGTGGCGACCTGGTTTACTATCAGGGCCACATTGCCCCCGGTATTTATAGCCGTGCTTTCCTCGAAGGTCGTCTGACC
GAAGAGCAGCTGGACAACTTCCGTCAGGAAGTGGACGGCAAGGGTATTTCGTCTTACCCACACCCCAAGCTGATGCCTGA
ATTCTGGCAATTCCCAACCGTATCCATGGGTCTGGGCCCTATCTCTTCTATTTATCAGGCACGCTTCCTCAAGTACCTGA
ATGGCCGTGGACTGAAAGACACCACCGAGCAGCGCGTGTACGCCTTCCTCGGCGATGGTGAAATGGACGAGCCGGAAAGC
CGCGGTGCTATCTCCTTCGCTGCCCGTGAGAAGCTGGACAACCTGTGCTTCCTCATCAACTGTAACCTGCAGCGTCTCGA
CGGCCCGGTAATGGGTAACGGCAAAATTATCCAGGAACTCGAAGGCCTGTTTAAAGGCGCGGGCTGGAACGTAATCAAGG
TAGTGTGGGGCAGTGGCTGGGACAAGCTGCTGGCCAAAGACACCACCGGCAAGCTGCTGCAGCTGATGAACGAAACCGTT
GACGGTGACTATCAGACCTTCAAGGCCAAAGATGGTGCCTACGTGCGTGAGCACTTCTTCGGTAAGTACCCAGAGACGGC
TGCTCTGGTTGCCGACATGACCGACGAAGAAATCTTCGCCCTAAAGCGCGGTGGCCATGAGCCTTCCAAGCTGTACGCGG
CATTCAAAGCTGCCCAGGAGACCAAGGGGCGTCCAACTGTGATCTTGGCCAAGACTGTAAAAGGTTACGGCATGGGCGAT
GCGGCCGAAGGCAAGAACATCGCGCACCAGGTGAAAAAGATGGACATGTCCCACGTGGCCAAGCTGCGTGACCGTCTGGG
TGTGACGGATCTGGTCAGCGACGAAGATCTGGTTAATCTGCCATACATCAAGCTCGAAGAAGGCAGTGTTGAGCACACCT
ATTTGCACGCCCGTCGTGAAGCCCTGCACGGCTACACGCCCAAGCGTCTGCCTAACTTCACCGGCGAGTTTGTTGCCCCT
GAACTGTCTGAGTTCAAGCCGCTGCTGGAAGAGCAGAAGCGTGATATTTCTACCACCATGGGCTTTGTTCGTACCCTGAA
CATCCTGCTCAAGGACAAGAATATCGGCAAAAACATCGTGCCTATTATCGCCGACGAAGCCCGTACCTTCGGTATGGAAG
GTCTGTTCCGTCAGATTGGTATCTATAACCCCTACGGCCAGAACTACATTCCGGAAGACCGCGATATCGTGTCTTACTAC
AAGGAAGACACTTCAGGCCAGGTACTGCAAGAAGGTATCAACGAGCTGGGCGCCATGTCTTCATGGGTTGCCGCTGCCAC
CTCGTACAGCACCAACAACCTGCCAATGATCCCGTTCTACATCTACTACTCCATGTTCGGCTTCCAGCGTGTGGGCGATA
TGGCGTGGATGGCAGGCGACCAGCAGGCGCGCGGCTTCCTTTTGGGTGCCACTGCCGGTCGTACCACCTTGAACGGTGAA
GGTCTGCAGCACGAAGACGGTCACAGCCACATCCTGGCCGGTACCGTACCTAACTGTATTTCTTACGATCCGACCTTTGC
TTACGAAACTGCCGTGATCATTCAGGACGGTATCCGCCGCATGTATGGCGAGCAGGAGAACGTGTTCTACTACCTGACCC
TGATGAACGAAAACTACGCCATGCCAGCCATGCCAGAAGGTGCCGAGGAAGGTATCCGCAAGGGTATCTACAAACTCGAA
AGCCATACAGGTGGCAAGGGCAGTGCCTACAGCCCAGCGAAGCCATCAAAAGTGCAGTTGATGAGCTCAGGCACCATCAT
GAATGAAGTACGTAAAGCCGCGGTTATCCTGGCTGAAGAATACGGCATAGGTTCAGATGTGTTCTCCGTGACTTCGTTCA
ACGAGCTGGCCCGTAACGGTCAGGACTGCGAGCGTTTCAATATGCTGCATCCTGAAGCTGACGCCCGCGTACCTTACATC
ACCTCAGTACTGGGCAGCGAGCCTGCCATTGCCGCGACCGACTACATGAAGAACTACGCCGAACAGGTTCGTGCCTTCAT
GCCAAGCGTGTCCTACAAGGTGCTGGGTACCGACGGTTTCGGTCGCTCTGACAGCCGCGCCAACCTGCGTCGTCACTTCG
AAGTGAACGCCGGTTACGTGGTGGTTGCAGCCCTGAACGAGCTGGCCAAGCGCGGTGACATCGAGAAATCTGTGGTGGTT
GCTGCCATCAAGAAGTTCGATATCGACACCGAGAAGATGAACCCACTTTACGCGTAA

Upstream 100 bases:

>100_bases
CCTGCGCCGATTAAAGAGTGGCGCCTGATGGCCGGGATGGTGTTTACCAGTACCTTGCAAGCCACTTTTCCCATAAATAT
GTATATATAAGGACAGTCTC

Downstream 100 bases:

>100_bases
GAGACGAGACAATGGCAATCGAAATTCATGTACCCGATATCGGCACTGATGAAGTCGAAGTGACCGAGATCCTGGTCAAG
GTGGGTGATTCCATCACTGA

Product: pyruvate dehydrogenase subunit E1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 898; Mature: 897

Protein sequence:

>898_residues
MSEPMQMLQDLDPIETQEWLAALESVVREEGVERAQFLLEQVLEQARLDGVDMPTGITTNYVNTIPLTQEPAYPGDITIE
RRIRSIIRWNAIMIVLRASKKDLELGGHMASFQSAAAFYEVCFNHFFRAPNHKDGGDLVYYQGHIAPGIYSRAFLEGRLT
EEQLDNFRQEVDGKGISSYPHPKLMPEFWQFPTVSMGLGPISSIYQARFLKYLNGRGLKDTTEQRVYAFLGDGEMDEPES
RGAISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFKGAGWNVIKVVWGSGWDKLLAKDTTGKLLQLMNETV
DGDYQTFKAKDGAYVREHFFGKYPETAALVADMTDEEIFALKRGGHEPSKLYAAFKAAQETKGRPTVILAKTVKGYGMGD
AAEGKNIAHQVKKMDMSHVAKLRDRLGVTDLVSDEDLVNLPYIKLEEGSVEHTYLHARREALHGYTPKRLPNFTGEFVAP
ELSEFKPLLEEQKRDISTTMGFVRTLNILLKDKNIGKNIVPIIADEARTFGMEGLFRQIGIYNPYGQNYIPEDRDIVSYY
KEDTSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYYSMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGE
GLQHEDGHSHILAGTVPNCISYDPTFAYETAVIIQDGIRRMYGEQENVFYYLTLMNENYAMPAMPEGAEEGIRKGIYKLE
SHTGGKGSAYSPAKPSKVQLMSSGTIMNEVRKAAVILAEEYGIGSDVFSVTSFNELARNGQDCERFNMLHPEADARVPYI
TSVLGSEPAIAATDYMKNYAEQVRAFMPSVSYKVLGTDGFGRSDSRANLRRHFEVNAGYVVVAALNELAKRGDIEKSVVV
AAIKKFDIDTEKMNPLYA

Sequences:

>Translated_898_residues
MSEPMQMLQDLDPIETQEWLAALESVVREEGVERAQFLLEQVLEQARLDGVDMPTGITTNYVNTIPLTQEPAYPGDITIE
RRIRSIIRWNAIMIVLRASKKDLELGGHMASFQSAAAFYEVCFNHFFRAPNHKDGGDLVYYQGHIAPGIYSRAFLEGRLT
EEQLDNFRQEVDGKGISSYPHPKLMPEFWQFPTVSMGLGPISSIYQARFLKYLNGRGLKDTTEQRVYAFLGDGEMDEPES
RGAISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFKGAGWNVIKVVWGSGWDKLLAKDTTGKLLQLMNETV
DGDYQTFKAKDGAYVREHFFGKYPETAALVADMTDEEIFALKRGGHEPSKLYAAFKAAQETKGRPTVILAKTVKGYGMGD
AAEGKNIAHQVKKMDMSHVAKLRDRLGVTDLVSDEDLVNLPYIKLEEGSVEHTYLHARREALHGYTPKRLPNFTGEFVAP
ELSEFKPLLEEQKRDISTTMGFVRTLNILLKDKNIGKNIVPIIADEARTFGMEGLFRQIGIYNPYGQNYIPEDRDIVSYY
KEDTSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYYSMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGE
GLQHEDGHSHILAGTVPNCISYDPTFAYETAVIIQDGIRRMYGEQENVFYYLTLMNENYAMPAMPEGAEEGIRKGIYKLE
SHTGGKGSAYSPAKPSKVQLMSSGTIMNEVRKAAVILAEEYGIGSDVFSVTSFNELARNGQDCERFNMLHPEADARVPYI
TSVLGSEPAIAATDYMKNYAEQVRAFMPSVSYKVLGTDGFGRSDSRANLRRHFEVNAGYVVVAALNELAKRGDIEKSVVV
AAIKKFDIDTEKMNPLYA
>Mature_897_residues
SEPMQMLQDLDPIETQEWLAALESVVREEGVERAQFLLEQVLEQARLDGVDMPTGITTNYVNTIPLTQEPAYPGDITIER
RIRSIIRWNAIMIVLRASKKDLELGGHMASFQSAAAFYEVCFNHFFRAPNHKDGGDLVYYQGHIAPGIYSRAFLEGRLTE
EQLDNFRQEVDGKGISSYPHPKLMPEFWQFPTVSMGLGPISSIYQARFLKYLNGRGLKDTTEQRVYAFLGDGEMDEPESR
GAISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFKGAGWNVIKVVWGSGWDKLLAKDTTGKLLQLMNETVD
GDYQTFKAKDGAYVREHFFGKYPETAALVADMTDEEIFALKRGGHEPSKLYAAFKAAQETKGRPTVILAKTVKGYGMGDA
AEGKNIAHQVKKMDMSHVAKLRDRLGVTDLVSDEDLVNLPYIKLEEGSVEHTYLHARREALHGYTPKRLPNFTGEFVAPE
LSEFKPLLEEQKRDISTTMGFVRTLNILLKDKNIGKNIVPIIADEARTFGMEGLFRQIGIYNPYGQNYIPEDRDIVSYYK
EDTSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYYSMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEG
LQHEDGHSHILAGTVPNCISYDPTFAYETAVIIQDGIRRMYGEQENVFYYLTLMNENYAMPAMPEGAEEGIRKGIYKLES
HTGGKGSAYSPAKPSKVQLMSSGTIMNEVRKAAVILAEEYGIGSDVFSVTSFNELARNGQDCERFNMLHPEADARVPYIT
SVLGSEPAIAATDYMKNYAEQVRAFMPSVSYKVLGTDGFGRSDSRANLRRHFEVNAGYVVVAALNELAKRGDIEKSVVVA
AIKKFDIDTEKMNPLYA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG2609

COG function: function code C; Pyruvate dehydrogenase complex, dehydrogenase (E1) component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786304, Length=891, Percent_Identity=73.1762065095398, Blast_Score=1385, Evalue=0.0,

Paralogues:

None

Copy number: 1140 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 400 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004660
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 100266; Mature: 100135

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPMQMLQDLDPIETQEWLAALESVVREEGVERAQFLLEQVLEQARLDGVDMPTGITTN
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
YVNTIPLTQEPAYPGDITIERRIRSIIRWNAIMIVLRASKKDLELGGHMASFQSAAAFYE
CEEECCCCCCCCCCCCEEHHHHHHHHHHHCEEEEEEECCCCCHHHCCCHHHHHHHHHHHH
VCFNHFFRAPNHKDGGDLVYYQGHIAPGIYSRAFLEGRLTEEQLDNFRQEVDGKGISSYP
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
HPKLMPEFWQFPTVSMGLGPISSIYQARFLKYLNGRGLKDTTEQRVYAFLGDGEMDEPES
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCCCC
RGAISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFKGAGWNVIKVVWGSGW
CCCCHHHHHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCH
DKLLAKDTTGKLLQLMNETVDGDYQTFKAKDGAYVREHFFGKYPETAALVADMTDEEIFA
HHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCHHHHHCCCCCHHHHH
LKRGGHEPSKLYAAFKAAQETKGRPTVILAKTVKGYGMGDAAEGKNIAHQVKKMDMSHVA
HHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
KLRDRLGVTDLVSDEDLVNLPYIKLEEGSVEHTYLHARREALHGYTPKRLPNFTGEFVAP
HHHHHCCCHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
ELSEFKPLLEEQKRDISTTMGFVRTLNILLKDKNIGKNIVPIIADEARTFGMEGLFRQIG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEECHHHHHCHHHHHHHHC
IYNPYGQNYIPEDRDIVSYYKEDTSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFY
CCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IYYSMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEGLQHEDGHSHILAGTVPNCI
HHHHHHHHHHHCCHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
SYDPTFAYETAVIIQDGIRRMYGEQENVFYYLTLMNENYAMPAMPEGAEEGIRKGIYKLE
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
SHTGGKGSAYSPAKPSKVQLMSSGTIMNEVRKAAVILAEEYGIGSDVFSVTSFNELARNG
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
QDCERFNMLHPEADARVPYITSVLGSEPAIAATDYMKNYAEQVRAFMPSVSYKVLGTDGF
CCHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
GRSDSRANLRRHFEVNAGYVVVAALNELAKRGDIEKSVVVAAIKKFDIDTEKMNPLYA
CCCCCHHHHHHEEECCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
SEPMQMLQDLDPIETQEWLAALESVVREEGVERAQFLLEQVLEQARLDGVDMPTGITTN
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
YVNTIPLTQEPAYPGDITIERRIRSIIRWNAIMIVLRASKKDLELGGHMASFQSAAAFYE
CEEECCCCCCCCCCCCEEHHHHHHHHHHHCEEEEEEECCCCCHHHCCCHHHHHHHHHHHH
VCFNHFFRAPNHKDGGDLVYYQGHIAPGIYSRAFLEGRLTEEQLDNFRQEVDGKGISSYP
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
HPKLMPEFWQFPTVSMGLGPISSIYQARFLKYLNGRGLKDTTEQRVYAFLGDGEMDEPES
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCCCC
RGAISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFKGAGWNVIKVVWGSGW
CCCCHHHHHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCH
DKLLAKDTTGKLLQLMNETVDGDYQTFKAKDGAYVREHFFGKYPETAALVADMTDEEIFA
HHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCHHHHHCCCCCHHHHH
LKRGGHEPSKLYAAFKAAQETKGRPTVILAKTVKGYGMGDAAEGKNIAHQVKKMDMSHVA
HHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
KLRDRLGVTDLVSDEDLVNLPYIKLEEGSVEHTYLHARREALHGYTPKRLPNFTGEFVAP
HHHHHCCCHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
ELSEFKPLLEEQKRDISTTMGFVRTLNILLKDKNIGKNIVPIIADEARTFGMEGLFRQIG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEECHHHHHCHHHHHHHHC
IYNPYGQNYIPEDRDIVSYYKEDTSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFY
CCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IYYSMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEGLQHEDGHSHILAGTVPNCI
HHHHHHHHHHHCCHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
SYDPTFAYETAVIIQDGIRRMYGEQENVFYYLTLMNENYAMPAMPEGAEEGIRKGIYKLE
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
SHTGGKGSAYSPAKPSKVQLMSSGTIMNEVRKAAVILAEEYGIGSDVFSVTSFNELARNG
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
QDCERFNMLHPEADARVPYITSVLGSEPAIAATDYMKNYAEQVRAFMPSVSYKVLGTDGF
CCHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
GRSDSRANLRRHFEVNAGYVVVAALNELAKRGDIEKSVVVAAIKKFDIDTEKMNPLYA
CCCCCHHHHHHEEECCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]