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The map label for this gene is murD [H]

Identifier: 119773493

GI number: 119773493

Start: 421842

End: 423173

Strand: Direct

Name: murD [H]

Synonym: Sama_0352

Alternate gene names: 119773493

Gene position: 421842-423173 (Clockwise)

Preceding gene: 119773492

Following gene: 119773494

Centisome position: 9.8

GC content: 57.43

Gene sequence:

>1332_bases
ATGGGTCTGCCACTGGATAAGCCACACTCACACGTCGTTCTGGGCCTTGGTGCCACGGGACTGTCTGTGGTGCGCTTTCT
GGCACGCAAAGGCATAGTGCCGCTGGTGATGGACAGCCGTCGCCAGCCGCCGGGTATGGATGTACTCAGTGCCGAGTTTC
CGGATGTTACGCTGGTTACGGGGGGCTTCGACTGCCGCTATCTGGTGCAGGCTCAGAGCATCATCATCAGCCCGGGTATT
GCCGTCGATACCCCAGAGGTGCGTGCGGCCATGGACATGGGCATTGAAGTGATTGGCGATGTCGAGCTTTTTGCCCGCGA
AATACAGGACATGGCGCCCTGCGTGCTGGCCATTACAGGCTCCAATGGCAAGTCAACCGTGACTACTCTGGTGGGGGAAA
TGGCCAGGGCTGATAACAAGGCCGTAGCCGTTGGCGGCAATATTGGCGTACCTGCGCTGGAGCTGCTGGGCAAAGGCGCC
GAGCTCTTTGTGCTGGAACTGTCCAGCTTCCAACTGGAAACCACCCACAGTCTTCATTGTATTGCAGGCACCTGCCTGAA
CATCAGTGAAGACCATATGGACAGGTACACAGATCTTGAAGCCTATCGTCAGGCCAAATTGCGGCTCTATCCGCAAAGTA
AGCTCAGCGTCTACAACCGGGACGATGCGGCGACCATACCTGACGAACCACGCAATACCGTCAGTTTTGGCTTGGGTGTA
CCCGAGCTTGATGACTGGGGGCTGATGGATGGCAAAATTTACCACGGCGCCAGTGAAATCATGAATTTATTGGACGTGGC
GTTGATTGGCAGCCATAACCACGCCAACCTGCTCGCCGCCATGGCACTGTGCGAAGCCGCAGGTATTTCCCGCGAGGCCA
TGGTGAAGGTCGCGAAGGAATTTACCGGGCTGGCGCACCGCTGCGAATTGGTGGCAAGCCACAACAGCATTGCCTGGGTC
AACGACTCAAAGGCCACCAATGTGGGGGCAACTGTTGCAGCGCTCAATGGCCTTGCTGAACATCTGGGTGACATCATTCT
GATTGCCGGCGGTGATGGTAAAGGGGCCGACTTCGAACCTCTGCGCGGTGCGCTGGGGGCTGTTAGCCACCTTATTACCC
TGGGCCGCGATGGCGACAAGATTGCCGTGCTGAAAGAAGGCGCCATCAAGGTGGCAACCATGGCGGAAGCGGTTGAAAAG
GCAAAAGCCCTGGCAAACCCGGGTGACATTGTGCTCTTGTCTCCAGCCTGCGCCAGCCTGGACATGTACACCAACTTTAT
GGCCCGAGGGGATGATTTCAGGGCCAATGTGGAGCGGGTCTATGGCAACTGA

Upstream 100 bases:

>100_bases
AGCTCAAAGGCTGGCCGGAACCCCGGGTAATTGTCCGCTTCTGGATTATCTCCCTGTTCCTGGTGCTGCTGGGTCTGGCC
ACGCTGAAGCTGAGGTAATT

Downstream 100 bases:

>100_bases
CAGCAAACAGCTCACCCTGTTTGAGCGTAGCGGCCATTTCTGGACCAGCTGGCTAAGCGGACGCAGTGCGCCCGGCAACC
AGCTGTATGACCGTGCGCTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MGLPLDKPHSHVVLGLGATGLSVVRFLARKGIVPLVMDSRRQPPGMDVLSAEFPDVTLVTGGFDCRYLVQAQSIIISPGI
AVDTPEVRAAMDMGIEVIGDVELFAREIQDMAPCVLAITGSNGKSTVTTLVGEMARADNKAVAVGGNIGVPALELLGKGA
ELFVLELSSFQLETTHSLHCIAGTCLNISEDHMDRYTDLEAYRQAKLRLYPQSKLSVYNRDDAATIPDEPRNTVSFGLGV
PELDDWGLMDGKIYHGASEIMNLLDVALIGSHNHANLLAAMALCEAAGISREAMVKVAKEFTGLAHRCELVASHNSIAWV
NDSKATNVGATVAALNGLAEHLGDIILIAGGDGKGADFEPLRGALGAVSHLITLGRDGDKIAVLKEGAIKVATMAEAVEK
AKALANPGDIVLLSPACASLDMYTNFMARGDDFRANVERVYGN

Sequences:

>Translated_443_residues
MGLPLDKPHSHVVLGLGATGLSVVRFLARKGIVPLVMDSRRQPPGMDVLSAEFPDVTLVTGGFDCRYLVQAQSIIISPGI
AVDTPEVRAAMDMGIEVIGDVELFAREIQDMAPCVLAITGSNGKSTVTTLVGEMARADNKAVAVGGNIGVPALELLGKGA
ELFVLELSSFQLETTHSLHCIAGTCLNISEDHMDRYTDLEAYRQAKLRLYPQSKLSVYNRDDAATIPDEPRNTVSFGLGV
PELDDWGLMDGKIYHGASEIMNLLDVALIGSHNHANLLAAMALCEAAGISREAMVKVAKEFTGLAHRCELVASHNSIAWV
NDSKATNVGATVAALNGLAEHLGDIILIAGGDGKGADFEPLRGALGAVSHLITLGRDGDKIAVLKEGAIKVATMAEAVEK
AKALANPGDIVLLSPACASLDMYTNFMARGDDFRANVERVYGN
>Mature_442_residues
GLPLDKPHSHVVLGLGATGLSVVRFLARKGIVPLVMDSRRQPPGMDVLSAEFPDVTLVTGGFDCRYLVQAQSIIISPGIA
VDTPEVRAAMDMGIEVIGDVELFAREIQDMAPCVLAITGSNGKSTVTTLVGEMARADNKAVAVGGNIGVPALELLGKGAE
LFVLELSSFQLETTHSLHCIAGTCLNISEDHMDRYTDLEAYRQAKLRLYPQSKLSVYNRDDAATIPDEPRNTVSFGLGVP
ELDDWGLMDGKIYHGASEIMNLLDVALIGSHNHANLLAAMALCEAAGISREAMVKVAKEFTGLAHRCELVASHNSIAWVN
DSKATNVGATVAALNGLAEHLGDIILIAGGDGKGADFEPLRGALGAVSHLITLGRDGDKIAVLKEGAIKVATMAEAVEKA
KALANPGDIVLLSPACASLDMYTNFMARGDDFRANVERVYGN

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=437, Percent_Identity=50.5720823798627, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46850; Mature: 46719

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLPLDKPHSHVVLGLGATGLSVVRFLARKGIVPLVMDSRRQPPGMDVLSAEFPDVTLVT
CCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECCCCCEEEEE
GGFDCRYLVQAQSIIISPGIAVDTPEVRAAMDMGIEVIGDVELFAREIQDMAPCVLAITG
CCCCEEEEEEECEEEECCCCEECCHHHHHHHHCCCCEECCHHHHHHHHHHCCCEEEEEEC
SNGKSTVTTLVGEMARADNKAVAVGGNIGVPALELLGKGAELFVLELSSFQLETTHSLHC
CCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCEEEEEEECCEEEECCCCEEE
IAGTCLNISEDHMDRYTDLEAYRQAKLRLYPQSKLSVYNRDDAATIPDEPRNTVSFGLGV
EEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHCEEECCCCCCCCCCCCCCCEEECCCC
PELDDWGLMDGKIYHGASEIMNLLDVALIGSHNHANLLAAMALCEAAGISREAMVKVAKE
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
FTGLAHRCELVASHNSIAWVNDSKATNVGATVAALNGLAEHLGDIILIAGGDGKGADFEP
HHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHH
LRGALGAVSHLITLGRDGDKIAVLKEGAIKVATMAEAVEKAKALANPGDIVLLSPACASL
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
DMYTNFMARGDDFRANVERVYGN
HHHHHHHHCCCCHHCCHHHHCCC
>Mature Secondary Structure 
GLPLDKPHSHVVLGLGATGLSVVRFLARKGIVPLVMDSRRQPPGMDVLSAEFPDVTLVT
CCCCCCCCCEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECCCCCEEEEE
GGFDCRYLVQAQSIIISPGIAVDTPEVRAAMDMGIEVIGDVELFAREIQDMAPCVLAITG
CCCCEEEEEEECEEEECCCCEECCHHHHHHHHCCCCEECCHHHHHHHHHHCCCEEEEEEC
SNGKSTVTTLVGEMARADNKAVAVGGNIGVPALELLGKGAELFVLELSSFQLETTHSLHC
CCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCEEEEEEECCEEEECCCCEEE
IAGTCLNISEDHMDRYTDLEAYRQAKLRLYPQSKLSVYNRDDAATIPDEPRNTVSFGLGV
EEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHCEEECCCCCCCCCCCCCCCEEECCCC
PELDDWGLMDGKIYHGASEIMNLLDVALIGSHNHANLLAAMALCEAAGISREAMVKVAKE
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
FTGLAHRCELVASHNSIAWVNDSKATNVGATVAALNGLAEHLGDIILIAGGDGKGADFEP
HHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHH
LRGALGAVSHLITLGRDGDKIAVLKEGAIKVATMAEAVEKAKALANPGDIVLLSPACASL
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
DMYTNFMARGDDFRANVERVYGN
HHHHHHHHCCCCHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]