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The map label for this gene is lieB [H]

Identifier: 119717390

GI number: 119717390

Start: 3365367

End: 3366950

Strand: Reverse

Name: lieB [H]

Synonym: Noca_3166

Alternate gene names: 119717390

Gene position: 3366950-3365367 (Counterclockwise)

Preceding gene: 119717391

Following gene: 119717388

Centisome position: 67.53

GC content: 70.45

Gene sequence:

>1584_bases
ATGCGCAGAATCTCGGGCGTCGCCACGGCGGCGGTTCTCGCTCTCACCGCCGTCGGCATCCAGTCCGGCGCCAGCCAGGC
CGTGACCGAGCGGCCGAACGCGGTCCAGGCCCTGCTCGCCCATCCGGGCGCGGCGCTGGCCAGCAACGGAACGGCGTTCA
CGGTCACCCACACCGTGACCGACGCCGACGGCAGCACCCACGTCCGCATGGACCGCACCTACCGTGGCCTGCCCGTGCTC
GGTGGGGACCTGGTCGTCCACCGCGGTACCCAGGGCGGGTGGCGCGGGGTGAGCCAGACCCTCGAGAACGAGGTGCACGT
CTCGACGACGCCGGCGGTCGGGAAGGCCGCCGCGGCCGTCCGGGCGCTCGCGCCGGCGAAGGCGACGCGCGGCACCACCG
GCGCGAAGACGCAGTCGACCCGCCTGGTCGTCGACGCGACCACCGGCACTGCTCGGCTCGCGTGGGAGGTCATCACCGGC
GGCACCCAGCAGGACGGCACGCCGAGCCGGCTGGCGACGTACGTCGACGCCCGGACCGGCGCGGTGATCCGCCGCGAGCA
GCAGATCCAGACCGCGGACGGCTCGGGCCAGTCGCTCTACTCCGGGACCGTGCCGCTGCAGCTGACGCTGTCGGGCTCGA
CGTACCAGCTCAAGGACCCGACCCGGGGCAACACCTACACGACCGACATGGGCAACGCGAGCGACTCCCTCGGCTGCCAG
TACTTCGGCTTCAACTGCAAGACCGGCACCCTGTTCACCAGCCCGGACAACCTGTTCGGCAACGGCGCGACGAGCAGCCG
GGAGTCGGCCGCCGTGGACGCGCAGTACGGCACGAACATGACGTGGGACTTCTACAAGTCGACCTACGGGCGCAACGGGA
TCTTCGGGACCGGCGCCGGCTCCTACAACCGGGTCCACTACGGCAAGAACTACGTCAACGCGTTCTGGGACGGCACCAAG
ATGACGTACGGCGACGGCGACGGGACCAACTACGGACCGTTGGTCTCGCTGGACGTGGCCGGTCACGAGATGTCGCACGG
CGTCACCGAGAACACCGCCGGACTGGCCTACTCGGGCGAGTCCGGTGGTCTCAACGAGGCGACCTCGGACATCTTCGGCA
CGATGGTGGAGTTCTTCGCCGCCAACGCCAACGACCCGGGCGACTACCTGATCGGCGAGGAGTTCGACCTCAAGAACCAC
CTCGGCTTCCGGCGGATGGACAACCCGGCCTCGGACGGCAGCTCCTTCAACTGCTGGTCGTCGACCGTCGGGAGCGCCGA
CGTCCACTACTCCTCGGGCGTCGGGAACCACTTCTTCTACCTGCTCGCCGAGGGCTCGGGCGCCAAGACCATCGGCGGCG
TCGCCCACAACAGCCCGACCTGCAACGGCTCGACGGTGACCGGCATCGGCCGGGACGCCGCGAGCGCGATCTGGTACCGC
GCGCTCACGGTCTACATGACCTCCAGCACCAGCTACGCCGGCGCCCGCACCGCCACGTTGAACGCGGCGCGGGACCTGTA
CGGCGCGGGCAGCGCGCAGCAGAACGCCGTGGCCGCCGCGTGGAGCGCGGTCAGCGTCAACTGA

Upstream 100 bases:

>100_bases
TTGCAGGAATCCGCACATCGCAACGGCTTCCCGATCTCGTTGTGGCTGGCAGGCTACGTCTCCTTCGTAGTCCCACCCAT
CGATGAAGAAGGACTCTCAC

Downstream 100 bases:

>100_bases
CGGTCCCGGTGACCGAGCTCGGACTCGGGTGACCGAGCTCGGACTCCGGTGATCGAGCTTGTCGAGATCCCGGGGCATGC
GCAGGGCGGTGACCGTGGTG

Product: griselysin

Products: NA

Alternate protein names: LIE2 [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MRRISGVATAAVLALTAVGIQSGASQAVTERPNAVQALLAHPGAALASNGTAFTVTHTVTDADGSTHVRMDRTYRGLPVL
GGDLVVHRGTQGGWRGVSQTLENEVHVSTTPAVGKAAAAVRALAPAKATRGTTGAKTQSTRLVVDATTGTARLAWEVITG
GTQQDGTPSRLATYVDARTGAVIRREQQIQTADGSGQSLYSGTVPLQLTLSGSTYQLKDPTRGNTYTTDMGNASDSLGCQ
YFGFNCKTGTLFTSPDNLFGNGATSSRESAAVDAQYGTNMTWDFYKSTYGRNGIFGTGAGSYNRVHYGKNYVNAFWDGTK
MTYGDGDGTNYGPLVSLDVAGHEMSHGVTENTAGLAYSGESGGLNEATSDIFGTMVEFFAANANDPGDYLIGEEFDLKNH
LGFRRMDNPASDGSSFNCWSSTVGSADVHYSSGVGNHFFYLLAEGSGAKTIGGVAHNSPTCNGSTVTGIGRDAASAIWYR
ALTVYMTSSTSYAGARTATLNAARDLYGAGSAQQNAVAAAWSAVSVN

Sequences:

>Translated_527_residues
MRRISGVATAAVLALTAVGIQSGASQAVTERPNAVQALLAHPGAALASNGTAFTVTHTVTDADGSTHVRMDRTYRGLPVL
GGDLVVHRGTQGGWRGVSQTLENEVHVSTTPAVGKAAAAVRALAPAKATRGTTGAKTQSTRLVVDATTGTARLAWEVITG
GTQQDGTPSRLATYVDARTGAVIRREQQIQTADGSGQSLYSGTVPLQLTLSGSTYQLKDPTRGNTYTTDMGNASDSLGCQ
YFGFNCKTGTLFTSPDNLFGNGATSSRESAAVDAQYGTNMTWDFYKSTYGRNGIFGTGAGSYNRVHYGKNYVNAFWDGTK
MTYGDGDGTNYGPLVSLDVAGHEMSHGVTENTAGLAYSGESGGLNEATSDIFGTMVEFFAANANDPGDYLIGEEFDLKNH
LGFRRMDNPASDGSSFNCWSSTVGSADVHYSSGVGNHFFYLLAEGSGAKTIGGVAHNSPTCNGSTVTGIGRDAASAIWYR
ALTVYMTSSTSYAGARTATLNAARDLYGAGSAQQNAVAAAWSAVSVN
>Mature_527_residues
MRRISGVATAAVLALTAVGIQSGASQAVTERPNAVQALLAHPGAALASNGTAFTVTHTVTDADGSTHVRMDRTYRGLPVL
GGDLVVHRGTQGGWRGVSQTLENEVHVSTTPAVGKAAAAVRALAPAKATRGTTGAKTQSTRLVVDATTGTARLAWEVITG
GTQQDGTPSRLATYVDARTGAVIRREQQIQTADGSGQSLYSGTVPLQLTLSGSTYQLKDPTRGNTYTTDMGNASDSLGCQ
YFGFNCKTGTLFTSPDNLFGNGATSSRESAAVDAQYGTNMTWDFYKSTYGRNGIFGTGAGSYNRVHYGKNYVNAFWDGTK
MTYGDGDGTNYGPLVSLDVAGHEMSHGVTENTAGLAYSGESGGLNEATSDIFGTMVEFFAANANDPGDYLIGEEFDLKNH
LGFRRMDNPASDGSSFNCWSSTVGSADVHYSSGVGNHFFYLLAEGSGAKTIGGVAHNSPTCNGSTVTGIGRDAASAIWYR
ALTVYMTSSTSYAGARTATLNAARDLYGAGSAQQNAVAAAWSAVSVN

Specific function: A leucine-specific metalloprotease that plays a role in controlling the amount of leupeptin during colony development. Degrades leupeptin into three components, acetyl-leucine, leucine and argininal [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28A subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR007484
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR008757
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF04389 Peptidase_M28; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C; PF05547 Peptidase_M6 [H]

EC number: NA

Molecular weight: Translated: 54786; Mature: 54786

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRISGVATAAVLALTAVGIQSGASQAVTERPNAVQALLAHPGAALASNGTAFTVTHTVT
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCCCHHCCCCCEEEEEEEEE
DADGSTHVRMDRTYRGLPVLGGDLVVHRGTQGGWRGVSQTLENEVHVSTTPAVGKAAAAV
CCCCCEEEEECCCCCCCCEECCCEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHH
RALAPAKATRGTTGAKTQSTRLVVDATTGTARLAWEVITGGTQQDGTPSRLATYVDARTG
HHHCCCHHCCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCC
AVIRREQQIQTADGSGQSLYSGTVPLQLTLSGSTYQLKDPTRGNTYTTDMGNASDSLGCQ
CEEEHHHHHEECCCCCCEEEECCCEEEEEECCCEEEECCCCCCCEEEECCCCCCCCCCEE
YFGFNCKTGTLFTSPDNLFGNGATSSRESAAVDAQYGTNMTWDFYKSTYGRNGIFGTGAG
EEEEECCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCEEHHHHHHCCCCCEEECCCC
SYNRVHYGKNYVNAFWDGTKMTYGDGDGTNYGPLVSLDVAGHEMSHGVTENTAGLAYSGE
CCCEEECCCHHHHEEECCCEEEECCCCCCCCCCEEEEEECCCHHHCCCCCCCCCEEECCC
SGGLNEATSDIFGTMVEFFAANANDPGDYLIGEEFDLKNHLGFRRMDNPASDGSSFNCWS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHCCCCCCCCCCCCCCCCCCCHH
STVGSADVHYSSGVGNHFFYLLAEGSGAKTIGGVAHNSPTCNGSTVTGIGRDAASAIWYR
HCCCCCCEEECCCCCCEEEEEEECCCCCCEECCCCCCCCCCCCCEEECCCCHHHHHHHHE
ALTVYMTSSTSYAGARTATLNAARDLYGAGSAQQNAVAAAWSAVSVN
EEEEEEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MRRISGVATAAVLALTAVGIQSGASQAVTERPNAVQALLAHPGAALASNGTAFTVTHTVT
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCCCHHCCCCCEEEEEEEEE
DADGSTHVRMDRTYRGLPVLGGDLVVHRGTQGGWRGVSQTLENEVHVSTTPAVGKAAAAV
CCCCCEEEEECCCCCCCCEECCCEEEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHH
RALAPAKATRGTTGAKTQSTRLVVDATTGTARLAWEVITGGTQQDGTPSRLATYVDARTG
HHHCCCHHCCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCCC
AVIRREQQIQTADGSGQSLYSGTVPLQLTLSGSTYQLKDPTRGNTYTTDMGNASDSLGCQ
CEEEHHHHHEECCCCCCEEEECCCEEEEEECCCEEEECCCCCCCEEEECCCCCCCCCCEE
YFGFNCKTGTLFTSPDNLFGNGATSSRESAAVDAQYGTNMTWDFYKSTYGRNGIFGTGAG
EEEEECCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCEEHHHHHHCCCCCEEECCCC
SYNRVHYGKNYVNAFWDGTKMTYGDGDGTNYGPLVSLDVAGHEMSHGVTENTAGLAYSGE
CCCEEECCCHHHHEEECCCEEEECCCCCCCCCCEEEEEECCCHHHCCCCCCCCCEEECCC
SGGLNEATSDIFGTMVEFFAANANDPGDYLIGEEFDLKNHLGFRRMDNPASDGSSFNCWS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHCCCCCCCCCCCCCCCCCCCHH
STVGSADVHYSSGVGNHFFYLLAEGSGAKTIGGVAHNSPTCNGSTVTGIGRDAASAIWYR
HCCCCCCEEECCCCCCEEEEEEECCCCCCEECCCCCCCCCCCCCEEECCCCHHHHHHHHE
ALTVYMTSSTSYAGARTATLNAARDLYGAGSAQQNAVAAAWSAVSVN
EEEEEEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA