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The map label for this gene is murD [H]

Identifier: 119717289

GI number: 119717289

Start: 3262823

End: 3264289

Strand: Reverse

Name: murD [H]

Synonym: Noca_3065

Alternate gene names: 119717289

Gene position: 3264289-3262823 (Counterclockwise)

Preceding gene: 119717290

Following gene: 119717288

Centisome position: 65.47

GC content: 73.35

Gene sequence:

>1467_bases
ATGGATCTCGATCACCTCGGTCGTCACGACTCCTGGGAAGGGGTCCGCGCCGTCGTCGCCGGCTTCGGCGTCTCCGGCTT
CGCGGCCGCCGACAACCTCAACCACCTCGGTGCGAGCGTGACCGCCCTCGACGAGTCCGAGGCCGGCGATCGGCCCGCCA
AGGCCGAGCTGCTCGAGGTGCTCGGCGCCGACGTACGCCTCGGGGCCGGCACCACCACGACCCTGCCCGAGGACGTGGAC
CTGCTGGTCACCTCGCCCGGCTGGAAGCCGAGCGCGCCGCTGCTCGCGCAGGCCCGCGCGCGCAGGATCCCCATCTGGGG
CGAGGTCGAGCTGGCCTGGCGGCTGCGCGACCCCGACCACCGCACCCCCTGGCTGTGCGTGACCGGCACGAACGGCAAGA
CCACCACCGTGCAGATGCTCGACTCGATCCTGCGGGCCGCCGGCCTGCGCAGCATCGCGGTCGGCAACGTCGGCCTCCCG
ATCGTCGAGGCCGTGATGGACCCCGAGCCGTACGACGTGCTGGCCGTCGAGCTGTCCAGCTTCCAGCTGCACTACACCGA
CTCGATGAGCGCCGAGGCGGCCGCGGTGCTCAACGTCGCCGAGGACCACCTGGACTGGTACTCCTCCTTCGGGGCCTATG
CCGCCGACAAGGGCCGGATCTACGAGCGGGCCCAGAAGGCCTGCGTCTACAACGTCGCCGACCCCGAGACCGAGCGGCTG
GTCCGCGAGGCCGACGTGGTCGAGGGCGCCCGCGCCGTCGGGTTCACCCTCGGCATGCCCGGCGTCGGCATGGTGGGCCT
GGTCGAGGACATCCTGGCCGACCGGGCCTTCATCGAGCAGCGCGACACCAGCGCCGCGGAGCTGTGCACGATCTCCGACC
TGGCCCTGCACTCGCCGGGCGGTGCGGCGCCCCACAACATCGCCAACGCGCTCGCCGCCGCCGCCCTGGCCCGCGCCCAC
GGCGTCTCCCAGGGCGCGGTCCGCGACGGGCTGCGCGCCTTCCGCCCCGACCGGCACCGGATCGCCGTCGTCGGCGCGGC
CGGCGGCGTGACCTGGGTCGACGACTCCAAGGCCACCAACCCGCATGCCGCCCAGTCCTCGCTGCTGGCCTACGAGCCGG
TGGTGTGGATCGCCGGCGGGCTCGCCAAGGGGGCCCACTTCGACGACCTGGTGCGCGCCGTGCGCGACCGGCTGCGCGGC
GTGGTGCTGCTCGGCCGCGACCGGGACGTCATCGCGGGGGCGCTTTCGCGACACGCGCCGGATGTGCCCGTCATCGTCGT
CGAGGACGACGAGACTGAGGGCGATCACGGTGGACGTGCCATGGAGCGCGTAGTCGGAGCGGCGGCCGAGTTGGCCCGAC
CCGGCGACACGGTCCTGCTGGCACCCGGCTGCGCCTCCATGGACATGTTCACCAACTACGCCGAGCGCGGCGACGCGTTC
GCCGACGCCGTACGACGCAGGCTGTAG

Upstream 100 bases:

>100_bases
GGGAGCAGGTGACCGTGGTGATCCGGTTCTGGATCATCACCGGGATCTGTGTCGCCGCGGGCCTGGGCGTGTTCTACGCG
GAATGGGTCGCCGGCATCTG

Downstream 100 bases:

>100_bases
GCCGACCGCACGCAGACCGGACCCGACGGGAGGGGGTGGGGCATGACGACCGCGAACCCCCTGGACCCCGTCGAGCGGGC
GCGGCCGGTCGGCCGGCGCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MDLDHLGRHDSWEGVRAVVAGFGVSGFAAADNLNHLGASVTALDESEAGDRPAKAELLEVLGADVRLGAGTTTTLPEDVD
LLVTSPGWKPSAPLLAQARARRIPIWGEVELAWRLRDPDHRTPWLCVTGTNGKTTTVQMLDSILRAAGLRSIAVGNVGLP
IVEAVMDPEPYDVLAVELSSFQLHYTDSMSAEAAAVLNVAEDHLDWYSSFGAYAADKGRIYERAQKACVYNVADPETERL
VREADVVEGARAVGFTLGMPGVGMVGLVEDILADRAFIEQRDTSAAELCTISDLALHSPGGAAPHNIANALAAAALARAH
GVSQGAVRDGLRAFRPDRHRIAVVGAAGGVTWVDDSKATNPHAAQSSLLAYEPVVWIAGGLAKGAHFDDLVRAVRDRLRG
VVLLGRDRDVIAGALSRHAPDVPVIVVEDDETEGDHGGRAMERVVGAAAELARPGDTVLLAPGCASMDMFTNYAERGDAF
ADAVRRRL

Sequences:

>Translated_488_residues
MDLDHLGRHDSWEGVRAVVAGFGVSGFAAADNLNHLGASVTALDESEAGDRPAKAELLEVLGADVRLGAGTTTTLPEDVD
LLVTSPGWKPSAPLLAQARARRIPIWGEVELAWRLRDPDHRTPWLCVTGTNGKTTTVQMLDSILRAAGLRSIAVGNVGLP
IVEAVMDPEPYDVLAVELSSFQLHYTDSMSAEAAAVLNVAEDHLDWYSSFGAYAADKGRIYERAQKACVYNVADPETERL
VREADVVEGARAVGFTLGMPGVGMVGLVEDILADRAFIEQRDTSAAELCTISDLALHSPGGAAPHNIANALAAAALARAH
GVSQGAVRDGLRAFRPDRHRIAVVGAAGGVTWVDDSKATNPHAAQSSLLAYEPVVWIAGGLAKGAHFDDLVRAVRDRLRG
VVLLGRDRDVIAGALSRHAPDVPVIVVEDDETEGDHGGRAMERVVGAAAELARPGDTVLLAPGCASMDMFTNYAERGDAF
ADAVRRRL
>Mature_488_residues
MDLDHLGRHDSWEGVRAVVAGFGVSGFAAADNLNHLGASVTALDESEAGDRPAKAELLEVLGADVRLGAGTTTTLPEDVD
LLVTSPGWKPSAPLLAQARARRIPIWGEVELAWRLRDPDHRTPWLCVTGTNGKTTTVQMLDSILRAAGLRSIAVGNVGLP
IVEAVMDPEPYDVLAVELSSFQLHYTDSMSAEAAAVLNVAEDHLDWYSSFGAYAADKGRIYERAQKACVYNVADPETERL
VREADVVEGARAVGFTLGMPGVGMVGLVEDILADRAFIEQRDTSAAELCTISDLALHSPGGAAPHNIANALAAAALARAH
GVSQGAVRDGLRAFRPDRHRIAVVGAAGGVTWVDDSKATNPHAAQSSLLAYEPVVWIAGGLAKGAHFDDLVRAVRDRLRG
VVLLGRDRDVIAGALSRHAPDVPVIVVEDDETEGDHGGRAMERVVGAAAELARPGDTVLLAPGCASMDMFTNYAERGDAF
ADAVRRRL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=476, Percent_Identity=33.1932773109244, Blast_Score=159, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 51664; Mature: 51664

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDHLGRHDSWEGVRAVVAGFGVSGFAAADNLNHLGASVTALDESEAGDRPAKAELLEV
CCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHCCCEEEECCCCCCCCCCHHHHHHHH
LGADVRLGAGTTTTLPEDVDLLVTSPGWKPSAPLLAQARARRIPIWGEVELAWRLRDPDH
HCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCC
RTPWLCVTGTNGKTTTVQMLDSILRAAGLRSIAVGNVGLPIVEAVMDPEPYDVLAVELSS
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCEEEEEEEEE
FQLHYTDSMSAEAAAVLNVAEDHLDWYSSFGAYAADKGRIYERAQKACVYNVADPETERL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCHHHHH
VREADVVEGARAVGFTLGMPGVGMVGLVEDILADRAFIEQRDTSAAELCTISDLALHSPG
HHHHHHHHCHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHHHEECCC
GAAPHNIANALAAAALARAHGVSQGAVRDGLRAFRPDRHRIAVVGAAGGVTWVDDSKATN
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCC
PHAAQSSLLAYEPVVWIAGGLAKGAHFDDLVRAVRDRLRGVVLLGRDRDVIAGALSRHAP
CCHHHHHHHHCCCHHEEECCCCCCCCHHHHHHHHHHHHCEEEEECCCCHHHHHHHHCCCC
DVPVIVVEDDETEGDHGGRAMERVVGAAAELARPGDTVLLAPGCASMDMFTNYAERGDAF
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCHHH
ADAVRRRL
HHHHHHCC
>Mature Secondary Structure
MDLDHLGRHDSWEGVRAVVAGFGVSGFAAADNLNHLGASVTALDESEAGDRPAKAELLEV
CCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHCCCEEEECCCCCCCCCCHHHHHHHH
LGADVRLGAGTTTTLPEDVDLLVTSPGWKPSAPLLAQARARRIPIWGEVELAWRLRDPDH
HCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCC
RTPWLCVTGTNGKTTTVQMLDSILRAAGLRSIAVGNVGLPIVEAVMDPEPYDVLAVELSS
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCEEEEEEEEE
FQLHYTDSMSAEAAAVLNVAEDHLDWYSSFGAYAADKGRIYERAQKACVYNVADPETERL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCHHHHH
VREADVVEGARAVGFTLGMPGVGMVGLVEDILADRAFIEQRDTSAAELCTISDLALHSPG
HHHHHHHHCHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHHHEECCC
GAAPHNIANALAAAALARAHGVSQGAVRDGLRAFRPDRHRIAVVGAAGGVTWVDDSKATN
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCC
PHAAQSSLLAYEPVVWIAGGLAKGAHFDDLVRAVRDRLRGVVLLGRDRDVIAGALSRHAP
CCHHHHHHHHCCCHHEEECCCCCCCCHHHHHHHHHHHHCEEEEECCCCHHHHHHHHCCCC
DVPVIVVEDDETEGDHGGRAMERVVGAAAELARPGDTVLLAPGCASMDMFTNYAERGDAF
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCHHH
ADAVRRRL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA