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The map label for this gene is hrdB [H]

Identifier: 119717187

GI number: 119717187

Start: 3148288

End: 3149775

Strand: Reverse

Name: hrdB [H]

Synonym: Noca_2963

Alternate gene names: 119717187

Gene position: 3149775-3148288 (Counterclockwise)

Preceding gene: 119717188

Following gene: 119717182

Centisome position: 63.17

GC content: 69.56

Gene sequence:

>1488_bases
GTGTTCGTGTCCTCGAACGCGCGCAAGATGCTTCCTGCCGAGGTGCTCACGCACCCTGCCGTCAAGGCCCTCATCGAGCG
AGGTACGCCGACCGGCAGCATCACTCCGGAGGAGGTGCGCCAGGCCAGTGAGGACGCGGCCGTCGAGCCCCGCCACCTCA
AGGCGCTGCTCGGGCACCTGAGCTCGCTGGGCATCGCGGTCGAGATCCCCGTGACCAGCCGCGCTGTCGCGGCCACCTCG
GCCCGCAAGACCGCCACCGCCAAGACGGCGGCCGCCAAGAAGGCACCGGCGAAGGCGGCGGCCGCCCCCGCGAAGTCCGC
TCCCGCGAAGAAGGCGGCGCCGGCGAAGGCTGCGCCCCGCAAGGCTGCCGCCGAGGGTGCTGCCGGCACCGAGTCGGCCG
AGCAGACCGTGACGGTCGGCCCGGACGGCAAGAAGGTGCTGCCCGACCTGCCCGACGAGCAGTTCGAGAAGGACGTGGCC
GCCGACCCGAGCATCGCCGAGGACGAGAAGCAGGCGTCGTTCGTCGTCTCCGCGGCCGACGACACCGACGAGCCCGAGCA
GCAGGTCATGGTGGCCGGCGCCACCGCGGACCCGGTCAAGGACTACCTCAAGCAGATCGGCAAGGTCCCCCTGCTCAACG
CCGAGATGGAGGTCGAGCTCGCCAAGCGGATCGAGGCCGGCCTGTTCTCCGAGGAGAAGCTCGGGAAGGGCGGCAAGCTC
TCGGCGAAGGTGTCCGAGGAGCTGGAGTGGATCGCCGAGGACGGCCGGCGCGCGAAGAACCACCTGCTCGAGGCGAACCT
GCGGCTGGTCGTCTCCCTGGCGAAGCGCTACACGGGGCGCGGGATGCTGTTCCTGGACCTGATCCAGGAGGGCAACCTCG
GCCTGATCCGCGCGGTCGAGAAGTTCGACTACACCAAGGGCTACAAGTTCTCGACCTACGCCACGTGGTGGATCCGCCAG
GCGATCACCCGCGCGATGGCCGACCAGGCCCGCACCATCCGGATCCCGGTGCACATGGTCGAGGTCATCAACAAGCTGGC
CCGCGTGCAGCGCCAGATGCTCCAGGACCTGGGCCGCGAGCCCACTCCGGAGGAGCTGGCCAAGGAGCTCGACATGACCC
CCGAGAAGGTCATCGAGGTCCAGAAGTACGGCCGCGAGCCGATCTCGTTGCACACCCCCCTCGGCGAGGACGGTGACTCC
GAGTTCGGCGACCTGATCGAGGACTCCGAGGCGATCGTCCCGGCCGACGCCGTGTCGTTCACGCTCCTCCAGGAGCAGCT
GCACGCCGTCCTCGACACGCTCTCCGAGCGCGAGGCGGGCGTGGTCAGCATGCGCTTCGGCCTGACCGACGGCCAGCCGA
AGACCCTCGACGAGATCGGCAAGGTGTACGGCGTGACCCGGGAGCGGATCCGCCAGATCGAGTCGAAGACCATGTCCAAG
CTGCGGCACCCGTCGCGCTCCCAGGTGCTGCGCGACTACCTGGACTGA

Upstream 100 bases:

>100_bases
CTGGGATCTCTGGAGGCCCGTGGCAGAATGTGGTCCGCGGCTCTTGACGTCACCGGGCCTCGCCGCGCCCCGATCCGGTT
CTCACAGTCGTTGACGAGAG

Downstream 100 bases:

>100_bases
TCCGGCCTCGGCTCAGGGCGCTGCGTCGTACCCGAAGACCTGGTAGTCCTTCCGGTACAGGTCGGCCACCACGCGCTGGA
GCTCGGTCGACGCCAGCAGC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MFVSSNARKMLPAEVLTHPAVKALIERGTPTGSITPEEVRQASEDAAVEPRHLKALLGHLSSLGIAVEIPVTSRAVAATS
ARKTATAKTAAAKKAPAKAAAAPAKSAPAKKAAPAKAAPRKAAAEGAAGTESAEQTVTVGPDGKKVLPDLPDEQFEKDVA
ADPSIAEDEKQASFVVSAADDTDEPEQQVMVAGATADPVKDYLKQIGKVPLLNAEMEVELAKRIEAGLFSEEKLGKGGKL
SAKVSEELEWIAEDGRRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQ
AITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAKELDMTPEKVIEVQKYGREPISLHTPLGEDGDS
EFGDLIEDSEAIVPADAVSFTLLQEQLHAVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSK
LRHPSRSQVLRDYLD

Sequences:

>Translated_495_residues
MFVSSNARKMLPAEVLTHPAVKALIERGTPTGSITPEEVRQASEDAAVEPRHLKALLGHLSSLGIAVEIPVTSRAVAATS
ARKTATAKTAAAKKAPAKAAAAPAKSAPAKKAAPAKAAPRKAAAEGAAGTESAEQTVTVGPDGKKVLPDLPDEQFEKDVA
ADPSIAEDEKQASFVVSAADDTDEPEQQVMVAGATADPVKDYLKQIGKVPLLNAEMEVELAKRIEAGLFSEEKLGKGGKL
SAKVSEELEWIAEDGRRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQ
AITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAKELDMTPEKVIEVQKYGREPISLHTPLGEDGDS
EFGDLIEDSEAIVPADAVSFTLLQEQLHAVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSK
LRHPSRSQVLRDYLD
>Mature_495_residues
MFVSSNARKMLPAEVLTHPAVKALIERGTPTGSITPEEVRQASEDAAVEPRHLKALLGHLSSLGIAVEIPVTSRAVAATS
ARKTATAKTAAAKKAPAKAAAAPAKSAPAKKAAPAKAAPRKAAAEGAAGTESAEQTVTVGPDGKKVLPDLPDEQFEKDVA
ADPSIAEDEKQASFVVSAADDTDEPEQQVMVAGATADPVKDYLKQIGKVPLLNAEMEVELAKRIEAGLFSEEKLGKGGKL
SAKVSEELEWIAEDGRRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQ
AITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAKELDMTPEKVIEVQKYGREPISLHTPLGEDGDS
EFGDLIEDSEAIVPADAVSFTLLQEQLHAVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSK
LRHPSRSQVLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=61.8257261410788, Blast_Score=322, Evalue=5e-89,
Organism=Escherichia coli, GI1789098, Length=339, Percent_Identity=36.283185840708, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1789871, Length=298, Percent_Identity=27.8523489932886, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=29, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 53761; Mature: 53761

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVSSNARKMLPAEVLTHPAVKALIERGTPTGSITPEEVRQASEDAAVEPRHLKALLGHL
CCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
SSLGIAVEIPVTSRAVAATSARKTATAKTAAAKKAPAKAAAAPAKSAPAKKAAPAKAAPR
HHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCH
KAAAEGAAGTESAEQTVTVGPDGKKVLPDLPDEQFEKDVAADPSIAEDEKQASFVVSAAD
HHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHEEEEEECC
DTDEPEQQVMVAGATADPVKDYLKQIGKVPLLNAEMEVELAKRIEAGLFSEEKLGKGGKL
CCCCCCHHEEEECCCCHHHHHHHHHHCCCCEECCHHHHHHHHHHHHCCCCHHHCCCCCCC
SAKVSEELEWIAEDGRRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHH
KFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGRE
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCC
PTPEELAKELDMTPEKVIEVQKYGREPISLHTPLGEDGDSEFGDLIEDSEAIVPADAVSF
CCHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHHHHH
TLLQEQLHAVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSK
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRHPSRSQVLRDYLD
HCCCCHHHHHHHHCC
>Mature Secondary Structure
MFVSSNARKMLPAEVLTHPAVKALIERGTPTGSITPEEVRQASEDAAVEPRHLKALLGHL
CCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
SSLGIAVEIPVTSRAVAATSARKTATAKTAAAKKAPAKAAAAPAKSAPAKKAAPAKAAPR
HHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCH
KAAAEGAAGTESAEQTVTVGPDGKKVLPDLPDEQFEKDVAADPSIAEDEKQASFVVSAAD
HHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHEEEEEECC
DTDEPEQQVMVAGATADPVKDYLKQIGKVPLLNAEMEVELAKRIEAGLFSEEKLGKGGKL
CCCCCCHHEEEECCCCHHHHHHHHHHCCCCEECCHHHHHHHHHHHHCCCCHHHCCCCCCC
SAKVSEELEWIAEDGRRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCHHHHHHH
KFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLARVQRQMLQDLGRE
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCC
PTPEELAKELDMTPEKVIEVQKYGREPISLHTPLGEDGDSEFGDLIEDSEAIVPADAVSF
CCHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCHHHHHH
TLLQEQLHAVLDTLSEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSK
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRHPSRSQVLRDYLD
HCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8690707 [H]