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The map label for this gene is gyrB [H]

Identifier: 119717182

GI number: 119717182

Start: 3143447

End: 3145579

Strand: Reverse

Name: gyrB [H]

Synonym: Noca_2958

Alternate gene names: 119717182

Gene position: 3145579-3143447 (Counterclockwise)

Preceding gene: 119717187

Following gene: 119717181

Centisome position: 63.09

GC content: 69.25

Gene sequence:

>2133_bases
ATGGCCGGGCGGCTCCACGCCGCCCGGCGGACAGACCGGCACGACCGGACCGGCACGACCAGGAGCCCCACGATCGACAA
CACGTACAACGCAGCCCACCTCCTCGTCCTCGAGGGCCTGGAGGCGGTCCGCAAGCGTCCCGGGATGTACATCGGCTCGA
CCGACACCCGCGGGCTCATGCACTGCCTGTGGGAGATCATCGACAACGGTGTCGACGAGGCGCTCGGGGGGCACGCCAGC
CACGTCGAGGTGACCCTCCACCCCGACGGCTCGGCCGAGGTGTACGACGACGGGCGGGGCATCCCGACCGACAAGGAGCC
CAAGACCGGGCTGCCGGGTGTCGAGGTCGTCGCGACCAAGCTGCACGCGGGCGGCAAGTTCGGCGGCGGCTCCTACGTCG
CGACCGGTGGCCTGCACGGAGTCGGCCTCTCGGTCGTCAATGCGCTGGCCACCCGGATGGACATCGACGTGGACCGCTCA
CCGGCCCGCCAGGGCATCTCGTTCCGGCGCGGCGTCCCCGGGGTGTTCTCCGGCGACGGCCCGCAGGCCGACTTCGAGGC
GCGCTCGGGCCTGACCCGCAAGGGTTCCCGGGTCGCGAAGGGCCGATCCGGCACCCGCATCGTGTTCTGGCCGGACCGGC
AGATCTTCACCAAGGACGCCCAGTTCGTCTACGAGGACCTGGTCGCGCGGGCCCGACAGACCTCGTTCATCGTCCCGGGG
CTCGAGCTCGTCATCCGCGACCTGCGCGGCCCCGACCCGGTCGAGGAGAAGTTCCGCCACGACGGCGGCATCGCCGAGTT
CGCCGACTTCCTGGCGCCCGACGAGCCGGTCACCGACATCCTTCGGCTCCAAGGCACCGACACGTTCACCGAGACGGTCC
CGCTGCTCGACGAGAAGGGGCAGATGACGCCGCAGGAGGTGGAGCGCGAGCTGCAGGTCGACGTCGCGCTGCGGTGGGGC
ACCGGCTACGACACCGTGCTGCGCTCGTTCGTCAACGTGATCGCCACGCCCAAGGGCGGCACCCACGTCAGCGGCTTCGA
ACAGGCCGTGACCCGCACGTTCAACGAGGTGATGAAGTCCGCGAAGGTGCTCAAGGTCAACGACGACGACGTGATCAAGG
ACGACGTCCTCGAGGGCATGACCTGCGTGGTGACGGTCCGGCTCTCCGAGCCGCAGTTCGAGGGCCAGACCAAGGAGATC
CTCGGCACACCGGCGGCTCGGACCGTCGTCCGCCGCGTCGTCTCCGCGGAGCTGAAGAAGTTCCTCACCTCGACCAAGCG
CGCCGAGAAGGCCCAGGCGAAGCTGGTCATGGAGAAGGTCGCCGGGGCGGCCAAGACCCGGATCACCGCCCGCCAGCACA
AGGAGACCCAGCGCCGCAAGAACGCCCTGGAGTCCTCGGCACTGCCCTCCAAGCTCGCCGACTGCCGTGCCGCGGACAAC
GACCGCACCGAGCTGTTCATCGTCGAGGGCGACTCCGCGCTCGGCACCGCCAAGCTCGCGCGCAACTCGGAGTTCCAGGC
GCTGCTGCCGATCCGCGGCAAGATCCTCAACGTCCAGAAGGCCTCGGTCGGCGACATGCTGAAGAACGCCGAGTGCGCCT
CGATCATCCAGGTGGTGGGAGCCGGCTCGGGCCGCACCTTCGACCTCGACGCACGCCGCTACGGGCGGATCATCTTCATG
GCCGACGCCGACTCCGACGGCGCCCACATCCGCTGCCTGCTGGCGACGCTGTTCTACAAGTACATGCCCGAGCTGATCCA
GGAGGGCCGGGTCTACACCGCCGTCCCGCCGCTGCACCGGGTCGAGATCTCCAACCCGAAGAAGGGGATGGAGAAGTACG
TGTACACCTACTCCGACGACGAGCTGCAGCGCAAGCTCGCCGAGCTCAAGAAGAAGGGCGTGCGCTGGAAGGACCCGGTG
CAGCGCTACAAGGGTCTCGGCGAGATGGACGCCGACCAGCTGGCGGAGACGACGATGGACCCGCGGCACCGCACGCTGCG
CCGGATCACCATCGACAACGCCGAGGAGGCCGCCGGGGTCTTCGAGCTGCTGATGGGCTCGGACGTCGCGCCCCGCAAGG
AGTTCATCGTGCAGGGCGCCTACGAGGTCGACGTCGAGGCGATCGACGCCTGA

Upstream 100 bases:

>100_bases
GCTCGGGGAAGTCCTGTCCACCGTAGCCACGGCCTCCGCAGGCGAGCCTGCACCGGCGACCGGCGTGTCGCAGGTAGATT
CGAGGACGACACCCGCACGG

Downstream 100 bases:

>100_bases
CCCCACGCCCGACCAGCCCTCGCACCAGCGTCCCGGAGGAGGCCCGATGTCCGTGCCGTGGGTCGACCCGGACGTCGACC
TGCACGCCCCCGGGCAGCAC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase II subunit B [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MAGRLHAARRTDRHDRTGTTRSPTIDNTYNAAHLLVLEGLEAVRKRPGMYIGSTDTRGLMHCLWEIIDNGVDEALGGHAS
HVEVTLHPDGSAEVYDDGRGIPTDKEPKTGLPGVEVVATKLHAGGKFGGGSYVATGGLHGVGLSVVNALATRMDIDVDRS
PARQGISFRRGVPGVFSGDGPQADFEARSGLTRKGSRVAKGRSGTRIVFWPDRQIFTKDAQFVYEDLVARARQTSFIVPG
LELVIRDLRGPDPVEEKFRHDGGIAEFADFLAPDEPVTDILRLQGTDTFTETVPLLDEKGQMTPQEVERELQVDVALRWG
TGYDTVLRSFVNVIATPKGGTHVSGFEQAVTRTFNEVMKSAKVLKVNDDDVIKDDVLEGMTCVVTVRLSEPQFEGQTKEI
LGTPAARTVVRRVVSAELKKFLTSTKRAEKAQAKLVMEKVAGAAKTRITARQHKETQRRKNALESSALPSKLADCRAADN
DRTELFIVEGDSALGTAKLARNSEFQALLPIRGKILNVQKASVGDMLKNAECASIIQVVGAGSGRTFDLDARRYGRIIFM
ADADSDGAHIRCLLATLFYKYMPELIQEGRVYTAVPPLHRVEISNPKKGMEKYVYTYSDDELQRKLAELKKKGVRWKDPV
QRYKGLGEMDADQLAETTMDPRHRTLRRITIDNAEEAAGVFELLMGSDVAPRKEFIVQGAYEVDVEAIDA

Sequences:

>Translated_710_residues
MAGRLHAARRTDRHDRTGTTRSPTIDNTYNAAHLLVLEGLEAVRKRPGMYIGSTDTRGLMHCLWEIIDNGVDEALGGHAS
HVEVTLHPDGSAEVYDDGRGIPTDKEPKTGLPGVEVVATKLHAGGKFGGGSYVATGGLHGVGLSVVNALATRMDIDVDRS
PARQGISFRRGVPGVFSGDGPQADFEARSGLTRKGSRVAKGRSGTRIVFWPDRQIFTKDAQFVYEDLVARARQTSFIVPG
LELVIRDLRGPDPVEEKFRHDGGIAEFADFLAPDEPVTDILRLQGTDTFTETVPLLDEKGQMTPQEVERELQVDVALRWG
TGYDTVLRSFVNVIATPKGGTHVSGFEQAVTRTFNEVMKSAKVLKVNDDDVIKDDVLEGMTCVVTVRLSEPQFEGQTKEI
LGTPAARTVVRRVVSAELKKFLTSTKRAEKAQAKLVMEKVAGAAKTRITARQHKETQRRKNALESSALPSKLADCRAADN
DRTELFIVEGDSALGTAKLARNSEFQALLPIRGKILNVQKASVGDMLKNAECASIIQVVGAGSGRTFDLDARRYGRIIFM
ADADSDGAHIRCLLATLFYKYMPELIQEGRVYTAVPPLHRVEISNPKKGMEKYVYTYSDDELQRKLAELKKKGVRWKDPV
QRYKGLGEMDADQLAETTMDPRHRTLRRITIDNAEEAAGVFELLMGSDVAPRKEFIVQGAYEVDVEAIDA
>Mature_709_residues
AGRLHAARRTDRHDRTGTTRSPTIDNTYNAAHLLVLEGLEAVRKRPGMYIGSTDTRGLMHCLWEIIDNGVDEALGGHASH
VEVTLHPDGSAEVYDDGRGIPTDKEPKTGLPGVEVVATKLHAGGKFGGGSYVATGGLHGVGLSVVNALATRMDIDVDRSP
ARQGISFRRGVPGVFSGDGPQADFEARSGLTRKGSRVAKGRSGTRIVFWPDRQIFTKDAQFVYEDLVARARQTSFIVPGL
ELVIRDLRGPDPVEEKFRHDGGIAEFADFLAPDEPVTDILRLQGTDTFTETVPLLDEKGQMTPQEVERELQVDVALRWGT
GYDTVLRSFVNVIATPKGGTHVSGFEQAVTRTFNEVMKSAKVLKVNDDDVIKDDVLEGMTCVVTVRLSEPQFEGQTKEIL
GTPAARTVVRRVVSAELKKFLTSTKRAEKAQAKLVMEKVAGAAKTRITARQHKETQRRKNALESSALPSKLADCRAADND
RTELFIVEGDSALGTAKLARNSEFQALLPIRGKILNVQKASVGDMLKNAECASIIQVVGAGSGRTFDLDARRYGRIIFMA
DADSDGAHIRCLLATLFYKYMPELIQEGRVYTAVPPLHRVEISNPKKGMEKYVYTYSDDELQRKLAELKKKGVRWKDPVQ
RYKGLGEMDADQLAETTMDPRHRTLRRITIDNAEEAAGVFELLMGSDVAPRKEFIVQGAYEVDVEAIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=711, Percent_Identity=24.1912798874824, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI19913406, Length=706, Percent_Identity=24.2209631728045, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI48994957, Length=601, Percent_Identity=40.5990016638935, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=39.0769230769231, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535065, Length=703, Percent_Identity=22.1906116642959, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI212645845, Length=352, Percent_Identity=27.8409090909091, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=236, Percent_Identity=25.4237288135593, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=376, Percent_Identity=28.4574468085106, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI17136538, Length=675, Percent_Identity=23.7037037037037, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 78027; Mature: 77895

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00626 RCC1_2 ; PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRLHAARRTDRHDRTGTTRSPTIDNTYNAAHLLVLEGLEAVRKRPGMYIGSTDTRGLM
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHH
HCLWEIIDNGVDEALGGHASHVEVTLHPDGSAEVYDDGRGIPTDKEPKTGLPGVEVVATK
HHHHHHHHCCHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHH
LHAGGKFGGGSYVATGGLHGVGLSVVNALATRMDIDVDRSPARQGISFRRGVPGVFSGDG
HHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHCCCCCCCCCCC
PQADFEARSGLTRKGSRVAKGRSGTRIVFWPDRQIFTKDAQFVYEDLVARARQTSFIVPG
CCCCHHHHCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEECCC
LELVIRDLRGPDPVEEKFRHDGGIAEFADFLAPDEPVTDILRLQGTDTFTETVPLLDEKG
HHHHHHHCCCCCCHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHCCCCCCC
QMTPQEVERELQVDVALRWGTGYDTVLRSFVNVIATPKGGTHVSGFEQAVTRTFNEVMKS
CCCHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
AKVLKVNDDDVIKDDVLEGMTCVVTVRLSEPQFEGQTKEILGTPAARTVVRRVVSAELKK
CEEEEECCCCHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
FLTSTKRAEKAQAKLVMEKVAGAAKTRITARQHKETQRRKNALESSALPSKLADCRAADN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
DRTELFIVEGDSALGTAKLARNSEFQALLPIRGKILNVQKASVGDMLKNAECASIIQVVG
CCCEEEEEECCCCCCHHHHHCCCCCEEEECCCCEEEEECHHHHHHHHHCCHHHHHHHHHC
AGSGRTFDLDARRYGRIIFMADADSDGAHIRCLLATLFYKYMPELIQEGRVYTAVPPLHR
CCCCCEEEECHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
VEISNPKKGMEKYVYTYSDDELQRKLAELKKKGVRWKDPVQRYKGLGEMDADQLAETTMD
EECCCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHCCC
PRHRTLRRITIDNAEEAAGVFELLMGSDVAPRKEFIVQGAYEVDVEAIDA
HHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCHHHHCCCCEEECEEEECC
>Mature Secondary Structure 
AGRLHAARRTDRHDRTGTTRSPTIDNTYNAAHLLVLEGLEAVRKRPGMYIGSTDTRGLM
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHH
HCLWEIIDNGVDEALGGHASHVEVTLHPDGSAEVYDDGRGIPTDKEPKTGLPGVEVVATK
HHHHHHHHCCHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHH
LHAGGKFGGGSYVATGGLHGVGLSVVNALATRMDIDVDRSPARQGISFRRGVPGVFSGDG
HHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHCCCCCCCCCCC
PQADFEARSGLTRKGSRVAKGRSGTRIVFWPDRQIFTKDAQFVYEDLVARARQTSFIVPG
CCCCHHHHCCCCCCCCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEECCC
LELVIRDLRGPDPVEEKFRHDGGIAEFADFLAPDEPVTDILRLQGTDTFTETVPLLDEKG
HHHHHHHCCCCCCHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHCCCCCCC
QMTPQEVERELQVDVALRWGTGYDTVLRSFVNVIATPKGGTHVSGFEQAVTRTFNEVMKS
CCCHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
AKVLKVNDDDVIKDDVLEGMTCVVTVRLSEPQFEGQTKEILGTPAARTVVRRVVSAELKK
CEEEEECCCCHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
FLTSTKRAEKAQAKLVMEKVAGAAKTRITARQHKETQRRKNALESSALPSKLADCRAADN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
DRTELFIVEGDSALGTAKLARNSEFQALLPIRGKILNVQKASVGDMLKNAECASIIQVVG
CCCEEEEEECCCCCCHHHHHCCCCCEEEECCCCEEEEECHHHHHHHHHCCHHHHHHHHHC
AGSGRTFDLDARRYGRIIFMADADSDGAHIRCLLATLFYKYMPELIQEGRVYTAVPPLHR
CCCCCEEEECHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
VEISNPKKGMEKYVYTYSDDELQRKLAELKKKGVRWKDPVQRYKGLGEMDADQLAETTMD
EECCCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHCCC
PRHRTLRRITIDNAEEAAGVFELLMGSDVAPRKEFIVQGAYEVDVEAIDA
HHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCHHHHCCCCEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8863738; 10360571 [H]