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The map label for this gene is ispH [H]

Identifier: 119715315

GI number: 119715315

Start: 1134319

End: 1135383

Strand: Direct

Name: ispH [H]

Synonym: Noca_1075

Alternate gene names: 119715315

Gene position: 1134319-1135383 (Clockwise)

Preceding gene: 119715311

Following gene: 119715316

Centisome position: 22.75

GC content: 71.27

Gene sequence:

>1065_bases
ATGGCCAGTCTCGGGCACCGGGCCGACGGGCGGCGGGCGCTCTCCACAGGCCCGGTCCGTAGACTCGGGGTCATGACGGA
CCTCGCCACCCCACGCACGCTGACCGAGGCGGAGAAGTCGGTCCTGCTGGCCGCGCCGCGCGGCTACTGTGCCGGCGTCG
ACCGGGCGGTGATCACCGTCGAGAAGGCACTGGACCTGTACGGCGCGCCCGTCTACGTGCGCAAGCAGATCGTGCACAAC
AAGCACGTGGTCGCCGACCTCGAGTCCCGCGGCGCGATCTTCGTCGAGGAGCTCGACGAAGTTCCCGAGGGCCAGACCGT
GGTGTTCTCGGCGCACGGCGTCTCGCCCGAGGTGCACCGGCAGGCCGAGGAGCGATCGCTGAAGACGATCGACGCGACCT
GCCCGCTGGTGACCAAGGTCCACCACGAGGCGAAGCGGTTCGCGACCGAGGACTACGACATCCTCCTCATCGGCCACGCC
GGCCACGAGGAGGTCGAGGGCACCGCGGGCGAGGCCCCCGGGCACATCCAGCTCGTGCAGGGTCCCGCGGACGTCCCGAA
CATCAAGGTCCGCGACCCGAAGCGGGTCGCCTGGCTCTCCCAGACCACGCTGTCGGTCGACGAGACCCTCGAGACCGTCG
ACGCGATCCGGGAGCGGTTCCCCGACCTGCTCGACCCGCCGAGCGACGACATCTGCTACGCCACCCAGAACCGTCAGCTG
GCGGTCAAGGAGATCGCTCGTGACTCCGACCTCGTGATCGTGGTCGGGTCGGGCAACTCCTCCAACTCGGTGCGACTGGT
CGAGGTCGCGCTCGAGGCGGGCGCCCGCGCGGCGCACCGGGTCGACGACGCCTCCGAGATCGACGAGGCCTGGCTCGACG
GGGTGCGCACGGTGAGCGTCACCTCCGGCGCCAGCGTGCCAGAGGCGCTGGTCCAGGGCGTGCTCTCCTTCCTGGGCGAG
CGCGGGTACCCCGACGCTCGGGCGGTGCACAGCGCCGAGGAGTCGCTGATCTTCGCGCTGCCCCACGAGCTGCGGCGCGA
CCTCAAGGCCGCCGGGCGGAGCTGA

Upstream 100 bases:

>100_bases
CTCGAGCGAGCTCTCCAGGGCCATGGCCGGGACCCTACCGAGCAGCGCCGACAGACTGCTTCTTCCACGATGGGCCCGGG
CCCGGGGCCGGGGCCGGGGC

Downstream 100 bases:

>100_bases
GCACCCGGATGGCCCGCTACGTCGACGTACACCCCGAGAACCCCCAGTCCCGCCTGCTCGACCAGATCGCCGACGCGCTG
CGCGAGGACCAGCTGATCGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MASLGHRADGRRALSTGPVRRLGVMTDLATPRTLTEAEKSVLLAAPRGYCAGVDRAVITVEKALDLYGAPVYVRKQIVHN
KHVVADLESRGAIFVEELDEVPEGQTVVFSAHGVSPEVHRQAEERSLKTIDATCPLVTKVHHEAKRFATEDYDILLIGHA
GHEEVEGTAGEAPGHIQLVQGPADVPNIKVRDPKRVAWLSQTTLSVDETLETVDAIRERFPDLLDPPSDDICYATQNRQL
AVKEIARDSDLVIVVGSGNSSNSVRLVEVALEAGARAAHRVDDASEIDEAWLDGVRTVSVTSGASVPEALVQGVLSFLGE
RGYPDARAVHSAEESLIFALPHELRRDLKAAGRS

Sequences:

>Translated_354_residues
MASLGHRADGRRALSTGPVRRLGVMTDLATPRTLTEAEKSVLLAAPRGYCAGVDRAVITVEKALDLYGAPVYVRKQIVHN
KHVVADLESRGAIFVEELDEVPEGQTVVFSAHGVSPEVHRQAEERSLKTIDATCPLVTKVHHEAKRFATEDYDILLIGHA
GHEEVEGTAGEAPGHIQLVQGPADVPNIKVRDPKRVAWLSQTTLSVDETLETVDAIRERFPDLLDPPSDDICYATQNRQL
AVKEIARDSDLVIVVGSGNSSNSVRLVEVALEAGARAAHRVDDASEIDEAWLDGVRTVSVTSGASVPEALVQGVLSFLGE
RGYPDARAVHSAEESLIFALPHELRRDLKAAGRS
>Mature_353_residues
ASLGHRADGRRALSTGPVRRLGVMTDLATPRTLTEAEKSVLLAAPRGYCAGVDRAVITVEKALDLYGAPVYVRKQIVHNK
HVVADLESRGAIFVEELDEVPEGQTVVFSAHGVSPEVHRQAEERSLKTIDATCPLVTKVHHEAKRFATEDYDILLIGHAG
HEEVEGTAGEAPGHIQLVQGPADVPNIKVRDPKRVAWLSQTTLSVDETLETVDAIRERFPDLLDPPSDDICYATQNRQLA
VKEIARDSDLVIVVGSGNSSNSVRLVEVALEAGARAAHRVDDASEIDEAWLDGVRTVSVTSGASVPEALVQGVLSFLGER
GYPDARAVHSAEESLIFALPHELRRDLKAAGRS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=312, Percent_Identity=50.6410256410256, Blast_Score=319, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 38234; Mature: 38103

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLGHRADGRRALSTGPVRRLGVMTDLATPRTLTEAEKSVLLAAPRGYCAGVDRAVITV
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCEEEECCCCCCCCCCHHHHHH
EKALDLYGAPVYVRKQIVHNKHVVADLESRGAIFVEELDEVPEGQTVVFSAHGVSPEVHR
HHHHHHCCCCHHHHHHHHCCCCEEEECCCCCCEEEHHHHCCCCCCEEEEEECCCCHHHHH
QAEERSLKTIDATCPLVTKVHHEAKRFATEDYDILLIGHAGHEEVEGTAGEAPGHIQLVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
GPADVPNIKVRDPKRVAWLSQTTLSVDETLETVDAIRERFPDLLDPPSDDICYATQNRQL
CCCCCCCEEECCCHHEEEHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEECCCCCC
AVKEIARDSDLVIVVGSGNSSNSVRLVEVALEAGARAAHRVDDASEIDEAWLDGVRTVSV
HHHHHCCCCCEEEEEECCCCCCCEEEEEEEHHHCCHHHHHCCCHHHHHHHHHCCCEEEEE
TSGASVPEALVQGVLSFLGERGYPDARAVHSAEESLIFALPHELRRDLKAAGRS
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEECCHHHHHHHHHCCCC
>Mature Secondary Structure 
ASLGHRADGRRALSTGPVRRLGVMTDLATPRTLTEAEKSVLLAAPRGYCAGVDRAVITV
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCEEEECCCCCCCCCCHHHHHH
EKALDLYGAPVYVRKQIVHNKHVVADLESRGAIFVEELDEVPEGQTVVFSAHGVSPEVHR
HHHHHHCCCCHHHHHHHHCCCCEEEECCCCCCEEEHHHHCCCCCCEEEEEECCCCHHHHH
QAEERSLKTIDATCPLVTKVHHEAKRFATEDYDILLIGHAGHEEVEGTAGEAPGHIQLVQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
GPADVPNIKVRDPKRVAWLSQTTLSVDETLETVDAIRERFPDLLDPPSDDICYATQNRQL
CCCCCCCEEECCCHHEEEHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEECCCCCC
AVKEIARDSDLVIVVGSGNSSNSVRLVEVALEAGARAAHRVDDASEIDEAWLDGVRTVSV
HHHHHCCCCCEEEEEECCCCCCCEEEEEEEHHHCCHHHHHCCCHHHHHHHHHCCCEEEEE
TSGASVPEALVQGVLSFLGERGYPDARAVHSAEESLIFALPHELRRDLKAAGRS
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA