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The map label for this gene is cobQ

Identifier: 119386421

GI number: 119386421

Start: 861405

End: 862838

Strand: Direct

Name: cobQ

Synonym: Pden_3713

Alternate gene names: 119386421

Gene position: 861405-862838 (Clockwise)

Preceding gene: 119386409

Following gene: 119386426

Centisome position: 49.79

GC content: 71.41

Gene sequence:

>1434_bases
ATGGGCGCGCTGATGATCCAGGGGACGGGGTCGAATGTGGGGAAATCCCTGCTGGTGGCGGGGCTTTGCCGGGCTGCAAG
GCGGCGCGGGATCGACGTCGCGCCCTTCAAGCCGCAGAACATGTCGAACAATGCCGCCGTGACCCCCGACGGGGGAGAGA
TCGGTCGGGCGCAGGCGCTGCAGGCGCGGGCGGCGGGGCTGGCGCCTTCGGTCCACATGAACCCGGTGCTGCTGAAGCCC
GAGACCGACCGCGCCGCCCAGGTGGTGGTGCAGGGCCGTGCCGCGACGCGGGCGGCGGCGGCGGATTACGGGGCGCTCAA
GGCGCGGCTGATGGGAGCGGTACTGGACAGCTTCGCGCGGCTGCGGACGACGCATGAGCTGGTGCTGGTCGAGGGCGCAG
GCTCGCCGGCCGAGGTCAACCTGCGCGCCCGCGACATCGCCAACATGGGCTTCGCCCGCGCTGCCGACGTGCCGGTGGTT
CTGGCCGGCGACATCGACCGCGGGGGCGTGATCGCGCAGATCGTCGGCACCCAGGCGGTGATCGACCCGGCTGACGCGGC
GATGATCCGCGGCTTCGTCATCAACCGCTTTCGCGGCGATCCCTCGCTGTTCGATGCCGGCCGCCGCTTCATCGTCGAAC
GCACCGGCTGGCCCGACCTGGGGCTGGTGCCGTGGTTCCCCGATGCCGTGCGGCTGCCGGCCGAGGATGCGGTAGATCTG
CGCCGTGCCTCGGGGGGCGGAGGCCTGCACATCGCCTGCCCGATGCTGTCGCGGATCGCCAATTTCGACGATCTCGACCC
GCTCGCGGCCGAGCCGGCGGTGCGGTTGAGCATGGTGCCGCCGGGCCATGCCCTGCCGGGCGATGCCGATCTGGTCATCC
TGCCGGGCACGAAATCCACCCGGGGCGACCTGGCCTTTCTGCGCGAACAGGGCTGGGATATCGACCTGGCCGCGCATCTG
CGGCGCGGTGGGCGGGTGCTGGGTATCTGCGGCGGCTATCAGATGCTGGGTCGGATGATCCACGATCCCGAGGGCCATGA
CGGCAGCCCCGGCAGCACGCCCGGCCTGGGCCTGCTGGATATCGAGACCCGCATGGCGCCCGAAAAGCGGCTGACGCGGA
TCGCGGGGCGCGCGCTCGGCCAGCCCGTCGAGGGCTACGAGATTCACATGGGCCGCAGCGAAGGCCCGGATTGCGCCCGT
CCCTTTGCCCATATCCCCGAGCCGGACGGTGCCACCAGCCCCGACGGACGCGTCGCCGGCACCTATCTGCATGGGCTCTT
CTCGGGCGATGGCTTTCGCGCCGCCTGGCTTGGGCAGTTCGGCGCTGCCTCGGCGCTGGATTACGGCGCCGGCGTCGATG
AGGTGCTCGACCGGCTGGCCGGGCACCTTGAGGCGCATCTGGACATCGACCGGCTTTTCGCGCTGGCGCGCTAG

Upstream 100 bases:

>100_bases
AGATGCACGGTAAGAACGATGTTTTCCACCACTGGGGCCTTTCATTCAGCGACGCGCCTATCTAGTCACCAAGGCGCGGA
TTGGCAAGCGGGGTGCGGGC

Downstream 100 bases:

>100_bases
AGGATTTCCGCCAGGATTCGCTCGGCCTCGGTCTGGCAGGGGGTCAGCTTCGCGCGATCCTCGCCCGGGAAAAAGCGCGC
GCGCACGGCCGTCGGCATCG

Product: cobyric acid synthase CobQ

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MGALMIQGTGSNVGKSLLVAGLCRAARRRGIDVAPFKPQNMSNNAAVTPDGGEIGRAQALQARAAGLAPSVHMNPVLLKP
ETDRAAQVVVQGRAATRAAAADYGALKARLMGAVLDSFARLRTTHELVLVEGAGSPAEVNLRARDIANMGFARAADVPVV
LAGDIDRGGVIAQIVGTQAVIDPADAAMIRGFVINRFRGDPSLFDAGRRFIVERTGWPDLGLVPWFPDAVRLPAEDAVDL
RRASGGGGLHIACPMLSRIANFDDLDPLAAEPAVRLSMVPPGHALPGDADLVILPGTKSTRGDLAFLREQGWDIDLAAHL
RRGGRVLGICGGYQMLGRMIHDPEGHDGSPGSTPGLGLLDIETRMAPEKRLTRIAGRALGQPVEGYEIHMGRSEGPDCAR
PFAHIPEPDGATSPDGRVAGTYLHGLFSGDGFRAAWLGQFGAASALDYGAGVDEVLDRLAGHLEAHLDIDRLFALAR

Sequences:

>Translated_477_residues
MGALMIQGTGSNVGKSLLVAGLCRAARRRGIDVAPFKPQNMSNNAAVTPDGGEIGRAQALQARAAGLAPSVHMNPVLLKP
ETDRAAQVVVQGRAATRAAAADYGALKARLMGAVLDSFARLRTTHELVLVEGAGSPAEVNLRARDIANMGFARAADVPVV
LAGDIDRGGVIAQIVGTQAVIDPADAAMIRGFVINRFRGDPSLFDAGRRFIVERTGWPDLGLVPWFPDAVRLPAEDAVDL
RRASGGGGLHIACPMLSRIANFDDLDPLAAEPAVRLSMVPPGHALPGDADLVILPGTKSTRGDLAFLREQGWDIDLAAHL
RRGGRVLGICGGYQMLGRMIHDPEGHDGSPGSTPGLGLLDIETRMAPEKRLTRIAGRALGQPVEGYEIHMGRSEGPDCAR
PFAHIPEPDGATSPDGRVAGTYLHGLFSGDGFRAAWLGQFGAASALDYGAGVDEVLDRLAGHLEAHLDIDRLFALAR
>Mature_476_residues
GALMIQGTGSNVGKSLLVAGLCRAARRRGIDVAPFKPQNMSNNAAVTPDGGEIGRAQALQARAAGLAPSVHMNPVLLKPE
TDRAAQVVVQGRAATRAAAADYGALKARLMGAVLDSFARLRTTHELVLVEGAGSPAEVNLRARDIANMGFARAADVPVVL
AGDIDRGGVIAQIVGTQAVIDPADAAMIRGFVINRFRGDPSLFDAGRRFIVERTGWPDLGLVPWFPDAVRLPAEDAVDLR
RASGGGGLHIACPMLSRIANFDDLDPLAAEPAVRLSMVPPGHALPGDADLVILPGTKSTRGDLAFLREQGWDIDLAAHLR
RGGRVLGICGGYQMLGRMIHDPEGHDGSPGSTPGLGLLDIETRMAPEKRLTRIAGRALGQPVEGYEIHMGRSEGPDCARP
FAHIPEPDGATSPDGRVAGTYLHGLFSGDGFRAAWLGQFGAASALDYGAGVDEVLDRLAGHLEAHLDIDRLFALAR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_PARDP (A1B8D6)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_917476.1
- ProteinModelPortal:   A1B8D6
- STRING:   A1B8D6
- GeneID:   4582265
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_3713
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 50122; Mature: 49991

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 330-330 ACT_SITE 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGALMIQGTGSNVGKSLLVAGLCRAARRRGIDVAPFKPQNMSNNAAVTPDGGEIGRAQAL
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHH
QARAAGLAPSVHMNPVLLKPETDRAAQVVVQGRAATRAAAADYGALKARLMGAVLDSFAR
HHHHHCCCCCCCCCEEEECCCCCCHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRTTHELVLVEGAGSPAEVNLRARDIANMGFARAADVPVVLAGDIDRGGVIAQIVGTQAV
HCCCEEEEEEECCCCCCEEEEEHHHHHHCCCCHHCCCCEEEECCCCCCCEEEEEECCCCE
IDPADAAMIRGFVINRFRGDPSLFDAGRRFIVERTGWPDLGLVPWFPDAVRLPAEDAVDL
ECCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCHHHCCCCHHHHHH
RRASGGGGLHIACPMLSRIANFDDLDPLAAEPAVRLSMVPPGHALPGDADLVILPGTKST
HHCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCC
RGDLAFLREQGWDIDLAAHLRRGGRVLGICGGYQMLGRMIHDPEGHDGSPGSTPGLGLLD
CCHHHHHHHCCCCCHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IETRMAPEKRLTRIAGRALGQPVEGYEIHMGRSEGPDCARPFAHIPEPDGATSPDGRVAG
EHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCEEH
TYLHGLFSGDGFRAAWLGQFGAASALDYGAGVDEVLDRLAGHLEAHLDIDRLFALAR
HEEHEEECCCCCEEEHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
GALMIQGTGSNVGKSLLVAGLCRAARRRGIDVAPFKPQNMSNNAAVTPDGGEIGRAQAL
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHH
QARAAGLAPSVHMNPVLLKPETDRAAQVVVQGRAATRAAAADYGALKARLMGAVLDSFAR
HHHHHCCCCCCCCCEEEECCCCCCHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRTTHELVLVEGAGSPAEVNLRARDIANMGFARAADVPVVLAGDIDRGGVIAQIVGTQAV
HCCCEEEEEEECCCCCCEEEEEHHHHHHCCCCHHCCCCEEEECCCCCCCEEEEEECCCCE
IDPADAAMIRGFVINRFRGDPSLFDAGRRFIVERTGWPDLGLVPWFPDAVRLPAEDAVDL
ECCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCHHHCCCCHHHHHH
RRASGGGGLHIACPMLSRIANFDDLDPLAAEPAVRLSMVPPGHALPGDADLVILPGTKST
HHCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCC
RGDLAFLREQGWDIDLAAHLRRGGRVLGICGGYQMLGRMIHDPEGHDGSPGSTPGLGLLD
CCHHHHHHHCCCCCHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IETRMAPEKRLTRIAGRALGQPVEGYEIHMGRSEGPDCARPFAHIPEPDGATSPDGRVAG
EHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCEEH
TYLHGLFSGDGFRAAWLGQFGAASALDYGAGVDEVLDRLAGHLEAHLDIDRLFALAR
HEEHEEECCCCCEEEHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA