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The map label for this gene is miaB

Identifier: 119386409

GI number: 119386409

Start: 850505

End: 851842

Strand: Direct

Name: miaB

Synonym: Pden_3701

Alternate gene names: 119386409

Gene position: 850505-851842 (Clockwise)

Preceding gene: 119386408

Following gene: 119386421

Centisome position: 49.16

GC content: 67.41

Gene sequence:

>1338_bases
ATGAGTGACCAAGCCAGCAAACCCGCCCCGGCGGTGAAGAAGCTCTTCATCAAGACCTATGGCTGCCAGATGAACGTCTA
TGACAGCCAGCGCATGGCCGAGGCCATGGGCGCCGAGGGCTATGTCCTGACCGAGAACCAGTCGGACGCCGACATGGTGC
TGCTGAACACCTGCCATATCCGGGAAAAAGCGGCCGAGAAGCTTTATTCCGACCTGGGGCGGCTGAAGCCCCTGAAGGCG
GAAAGGCCGGACCTGAAGATCGGCGTCGCCGGCTGCGTGGCCCAGGCCGAGGGCGAGGAGATCCAGCGCCGCATGCCCAT
CGTCGACCTGGTGGTGGGGCCGCAGGCCTATCACCGGCTGCCGGCCATGGCGCGCGCGGGCAGGGGCGTCGATACCGAAT
TCCCGGCCGAGGACAAGTTCGAGCATCTGCCGAAGCCCGCCGCCACCAGGCGCGCCCCGGCGGCTTTCCTGACCGTGCAG
GAGGGGTGCGACAAGTTCTGCGCCTTTTGCGTCGTGCCCTATACGCGGGGGGCCGAGGTGTCGCGCCCGGTCTCGCGCAT
CCTGGCCGAGGCGCGCGATCTGGTCGCGCGCGGCGTGCGCGAGATCACGCTTTTGGGCCAGAATGTCAACGGCTGGCATG
GCGAGGGGCCGGAGGGGTCCGAATGGGGTTTCGGCCGGCTGATCCGGGCGATCGCGGAAATCGACGGGCTGGACCGCATC
CGCTATACCACCAGCCATCCGAACGACATGGCCGACGACCTGATCGCGGCGCATCGCGATGAGCCGAAGCTGATGCCCTA
TCTGCACCTGCCGGTGCAATCGGGTTCCGACCGCATCCTCAAGGCGATGAACCGCCGCCACACGGTCGACCAATACCTGC
GGCTGATCGAGCGTATCCGCGAGGCGCGGCCCGACATCATGCTGACCAGCGATTTCATCGTCGGCTTCCCCGGCGAGACC
GACCAGGACCACCAGGGCACGCTGGAGCTGGTGCGGGCGGTGAATTTCGGCACCGCCTTCAGCTTCAAGTATTCGCCGCG
TCCCGGCACCCCGGCCTATGAGCGGCCGGAGATCGAGGGTGCCGTGGCCGATGCGCGCCTGCAGGAATTGCAGGCGTTGC
TGACCAGCCAGCAGAAGGCGGCGCAGGAGGGCATGGTCGGGCGCGAGCTGGGCGTGCTGTTCGAGAAGCCCGGCCGCAAT
CCGGGGCAGATGGTCGGCAAGTCGGACTATCTGCACGCGGTCTTTGTCGAGGCGCCGGCCGCCAAGGTCGGCGATCTGGT
GCGCGTGCGCATCACCCATAGCGCGCCGAACTCGCTGGCCGGGGTGTTGGCGGCCTGA

Upstream 100 bases:

>100_bases
TTTGGGGAACGAAGAAGCCGGGCGCCGGGCGTTATGGCGGCCGGCCTTTCCAAGCCGGGCGTTTTCCTGTATCAGGCGTC
GATCCCAAGGACAGCACGGC

Downstream 100 bases:

>100_bases
GCCGCCAGTGACGCTGCCGCCAGCGACAATGGTCAGACCGTGTCGAGGTAAAGCTCGACGGTTTCCTCGTCGGAAAGTTC
GGCCATGTAGTGCAGTTCCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSDQASKPAPAVKKLFIKTYGCQMNVYDSQRMAEAMGAEGYVLTENQSDADMVLLNTCHIREKAAEKLYSDLGRLKPLKA
ERPDLKIGVAGCVAQAEGEEIQRRMPIVDLVVGPQAYHRLPAMARAGRGVDTEFPAEDKFEHLPKPAATRRAPAAFLTVQ
EGCDKFCAFCVVPYTRGAEVSRPVSRILAEARDLVARGVREITLLGQNVNGWHGEGPEGSEWGFGRLIRAIAEIDGLDRI
RYTTSHPNDMADDLIAAHRDEPKLMPYLHLPVQSGSDRILKAMNRRHTVDQYLRLIERIREARPDIMLTSDFIVGFPGET
DQDHQGTLELVRAVNFGTAFSFKYSPRPGTPAYERPEIEGAVADARLQELQALLTSQQKAAQEGMVGRELGVLFEKPGRN
PGQMVGKSDYLHAVFVEAPAAKVGDLVRVRITHSAPNSLAGVLAA

Sequences:

>Translated_445_residues
MSDQASKPAPAVKKLFIKTYGCQMNVYDSQRMAEAMGAEGYVLTENQSDADMVLLNTCHIREKAAEKLYSDLGRLKPLKA
ERPDLKIGVAGCVAQAEGEEIQRRMPIVDLVVGPQAYHRLPAMARAGRGVDTEFPAEDKFEHLPKPAATRRAPAAFLTVQ
EGCDKFCAFCVVPYTRGAEVSRPVSRILAEARDLVARGVREITLLGQNVNGWHGEGPEGSEWGFGRLIRAIAEIDGLDRI
RYTTSHPNDMADDLIAAHRDEPKLMPYLHLPVQSGSDRILKAMNRRHTVDQYLRLIERIREARPDIMLTSDFIVGFPGET
DQDHQGTLELVRAVNFGTAFSFKYSPRPGTPAYERPEIEGAVADARLQELQALLTSQQKAAQEGMVGRELGVLFEKPGRN
PGQMVGKSDYLHAVFVEAPAAKVGDLVRVRITHSAPNSLAGVLAA
>Mature_444_residues
SDQASKPAPAVKKLFIKTYGCQMNVYDSQRMAEAMGAEGYVLTENQSDADMVLLNTCHIREKAAEKLYSDLGRLKPLKAE
RPDLKIGVAGCVAQAEGEEIQRRMPIVDLVVGPQAYHRLPAMARAGRGVDTEFPAEDKFEHLPKPAATRRAPAAFLTVQE
GCDKFCAFCVVPYTRGAEVSRPVSRILAEARDLVARGVREITLLGQNVNGWHGEGPEGSEWGFGRLIRAIAEIDGLDRIR
YTTSHPNDMADDLIAAHRDEPKLMPYLHLPVQSGSDRILKAMNRRHTVDQYLRLIERIREARPDIMLTSDFIVGFPGETD
QDHQGTLELVRAVNFGTAFSFKYSPRPGTPAYERPEIEGAVADARLQELQALLTSQQKAAQEGMVGRELGVLFEKPGRNP
GQMVGKSDYLHAVFVEAPAAKVGDLVRVRITHSAPNSLAGVLAA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=35.3065539112051, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI28872782, Length=473, Percent_Identity=35.3065539112051, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI93277076, Length=453, Percent_Identity=27.8145695364238, Blast_Score=188, Evalue=8e-48,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=49.8866213151927, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=31.140350877193, Blast_Score=192, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=458, Percent_Identity=32.5327510917031, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=297, Percent_Identity=27.9461279461279, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=30.7368421052632, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=26.4444444444444, Blast_Score=154, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PARDP (A1B8C4)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_917464.1
- ProteinModelPortal:   A1B8C4
- SMR:   A1B8C4
- STRING:   A1B8C4
- GeneID:   4582253
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_3701
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- PhylomeDB:   A1B8C4
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48977; Mature: 48845

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQASKPAPAVKKLFIKTYGCQMNVYDSQRMAEAMGAEGYVLTENQSDADMVLLNTCHI
CCCCCCCCCHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCEEEECCCCCCCEEEEEHHHH
REKAAEKLYSDLGRLKPLKAERPDLKIGVAGCVAQAEGEEIQRRMPIVDLVVGPQAYHRL
HHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHCCHHHHHHHCCEEEEEECCHHHHHH
PAMARAGRGVDTEFPAEDKFEHLPKPAATRRAPAAFLTVQEGCDKFCAFCVVPYTRGAEV
HHHHHCCCCCCCCCCCCCHHHHCCCCCHHCCCCHHEEEHHHHHHHHHHHHHCCCCCCCCH
SRPVSRILAEARDLVARGVREITLLGQNVNGWHGEGPEGSEWGFGRLIRAIAEIDGLDRI
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
RYTTSHPNDMADDLIAAHRDEPKLMPYLHLPVQSGSDRILKAMNRRHTVDQYLRLIERIR
EEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
EARPDIMLTSDFIVGFPGETDQDHQGTLELVRAVNFGTAFSFKYSPRPGTPAYERPEIEG
HCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCH
AVADARLQELQALLTSQQKAAQEGMVGRELGVLFEKPGRNPGQMVGKSDYLHAVFVEAPA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCHHHHCCCCCCEEEEEEECCC
AKVGDLVRVRITHSAPNSLAGVLAA
HHHCCEEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
SDQASKPAPAVKKLFIKTYGCQMNVYDSQRMAEAMGAEGYVLTENQSDADMVLLNTCHI
CCCCCCCCHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCEEEECCCCCCCEEEEEHHHH
REKAAEKLYSDLGRLKPLKAERPDLKIGVAGCVAQAEGEEIQRRMPIVDLVVGPQAYHRL
HHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHCCHHHHHHHCCEEEEEECCHHHHHH
PAMARAGRGVDTEFPAEDKFEHLPKPAATRRAPAAFLTVQEGCDKFCAFCVVPYTRGAEV
HHHHHCCCCCCCCCCCCCHHHHCCCCCHHCCCCHHEEEHHHHHHHHHHHHHCCCCCCCCH
SRPVSRILAEARDLVARGVREITLLGQNVNGWHGEGPEGSEWGFGRLIRAIAEIDGLDRI
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
RYTTSHPNDMADDLIAAHRDEPKLMPYLHLPVQSGSDRILKAMNRRHTVDQYLRLIERIR
EEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
EARPDIMLTSDFIVGFPGETDQDHQGTLELVRAVNFGTAFSFKYSPRPGTPAYERPEIEG
HCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCH
AVADARLQELQALLTSQQKAAQEGMVGRELGVLFEKPGRNPGQMVGKSDYLHAVFVEAPA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEECCCCCHHHHCCCCCCEEEEEEECCC
AKVGDLVRVRITHSAPNSLAGVLAA
HHHCCEEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA