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The map label for this gene is citA [H]

Identifier: 119386229

GI number: 119386229

Start: 664452

End: 665795

Strand: Direct

Name: citA [H]

Synonym: Pden_3518

Alternate gene names: 119386229

Gene position: 664452-665795 (Clockwise)

Preceding gene: 119386228

Following gene: 119386230

Centisome position: 38.41

GC content: 66.37

Gene sequence:

>1344_bases
ATGGAAACCACACGGCAGGACTGGCGGAGCGTGCCCGCCAAACGATCATCGCCGGGGCGCGCGGTCGCCGCGGCCTCGAT
CGGCAACGCGCTCGAATGGTACGACTTCACCGTCTATGCACTGTTCGCGATCTATATCGCGCAGAACTTCTTTCCGGGCG
GCGACGATACCGTCGAACTGGTCAAGGCCTTCCTGGCCTTCGGTCTCGGCTTCGTGATCCGCCCGCTGGGGGCCGTCGTG
ATCGGGGTCTATGGCGACAAGGCCGGCCGCAAGGCGGCGCTGTTTCTGACGATCATGATCATGGCGGCGGGGACGCTGCT
CATCGCAATTGCGCCGACCTATGCCGCGATGGGCATCGGCGCGCCGCTGATGATCCTTGCCGGCCGCGTTCTGCAGGGCT
TTTCGGCGGGTGGCGAGATCGGCGGCGCGGCGGCATTTCTGGTCGAGCATGCGCCGGCCGACAAGAAGGGCAAATACGCC
TCGTGGTTGCAGGCGAGCATGGCGATCTCGAACATCATGGGCGCGCTTGTGGCCTTCGCCGTCACATCGCTCCTGACCGA
GCAGCAGGTCGGGGACTGGGGCTGGCGCATACCCTTCTTCATCGGTGTCCTGATCGCGCCGGTCGGCCTGTGGCTGCGCA
AGACGCTGGACGAAACCCCGGCCTTCCAGGCCGAGCGGGCCAGCGCCGGGCGCGAACCCGCCGCCGCGCCGCTCAGGCAG
GTCTTCTCGGACTATCTCGGCCACCTGATCAAGGGCTTCGCCTTTTCGATCCTGTGGGCGGTCTGCGTCTACGTGCTGAT
CATCTACATGCCGATCTTCGTCCAGAGGGCCTTCGGCTTCACCCCCTCGCAGGCCTTCACCGCCTCGCTGATCGGCAACG
TCTTCCTGGCCGTGGGCTGTGTCTGCGCCGGCACCGTCTCGGACAGGATCGGGCGTCGCCTGATGCTGGGAGCCTCGGCG
GCGCTGATGCTGGTCGCGGTCTATCCGTTGATCTCGTGGCTTCAGGCATCGCCGACGCTGACCACCCTCGTCATCGTGCA
AAGCCTCTTTTGCATCATGGTCTCGGGCTTCGTGGGCGTGGCGCCCGCCACCCTTTCCGAAGTCTTTCCGACCCATGTCC
GCACCACCGGCATGTCGCTGGCCTACAATGCCGCCGTGACGATCTTCGGCGGGTTCGCCCCCGCGACGCTGACATGGCTG
GGCAGCACGGGGATCGGTTTCCATGCGCCCGCATTCTACGTCATGGCGGCCGCCGCCGCGTCGCTGATCGCGGTGCTGGT
GCTGCCAAAGCCCTATTCGACGAATTGCGGGCGCGGGGCCGTTCCGGCCCCCGCCGATCCTTGA

Upstream 100 bases:

>100_bases
CGCGGCAGCCAGAACCCGCCAAGGGTCCGCCTGCGCCCGGAAGAAGCCAAACAGCAATCAACCAGCGCCCGCGGTTACCG
ACGGGCAAAGGGGAGGAAAG

Downstream 100 bases:

>100_bases
TGCGGCAACCATGGAAAGGAAATCTCATGTCCGTTCGAACCGGAACCGGCCGCCACCACTGCCTGACCGCCCTTTGCCTG
ACGGCCGCGCTTGGCGGTTC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
METTRQDWRSVPAKRSSPGRAVAAASIGNALEWYDFTVYALFAIYIAQNFFPGGDDTVELVKAFLAFGLGFVIRPLGAVV
IGVYGDKAGRKAALFLTIMIMAAGTLLIAIAPTYAAMGIGAPLMILAGRVLQGFSAGGEIGGAAAFLVEHAPADKKGKYA
SWLQASMAISNIMGALVAFAVTSLLTEQQVGDWGWRIPFFIGVLIAPVGLWLRKTLDETPAFQAERASAGREPAAAPLRQ
VFSDYLGHLIKGFAFSILWAVCVYVLIIYMPIFVQRAFGFTPSQAFTASLIGNVFLAVGCVCAGTVSDRIGRRLMLGASA
ALMLVAVYPLISWLQASPTLTTLVIVQSLFCIMVSGFVGVAPATLSEVFPTHVRTTGMSLAYNAAVTIFGGFAPATLTWL
GSTGIGFHAPAFYVMAAAAASLIAVLVLPKPYSTNCGRGAVPAPADP

Sequences:

>Translated_447_residues
METTRQDWRSVPAKRSSPGRAVAAASIGNALEWYDFTVYALFAIYIAQNFFPGGDDTVELVKAFLAFGLGFVIRPLGAVV
IGVYGDKAGRKAALFLTIMIMAAGTLLIAIAPTYAAMGIGAPLMILAGRVLQGFSAGGEIGGAAAFLVEHAPADKKGKYA
SWLQASMAISNIMGALVAFAVTSLLTEQQVGDWGWRIPFFIGVLIAPVGLWLRKTLDETPAFQAERASAGREPAAAPLRQ
VFSDYLGHLIKGFAFSILWAVCVYVLIIYMPIFVQRAFGFTPSQAFTASLIGNVFLAVGCVCAGTVSDRIGRRLMLGASA
ALMLVAVYPLISWLQASPTLTTLVIVQSLFCIMVSGFVGVAPATLSEVFPTHVRTTGMSLAYNAAVTIFGGFAPATLTWL
GSTGIGFHAPAFYVMAAAAASLIAVLVLPKPYSTNCGRGAVPAPADP
>Mature_447_residues
METTRQDWRSVPAKRSSPGRAVAAASIGNALEWYDFTVYALFAIYIAQNFFPGGDDTVELVKAFLAFGLGFVIRPLGAVV
IGVYGDKAGRKAALFLTIMIMAAGTLLIAIAPTYAAMGIGAPLMILAGRVLQGFSAGGEIGGAAAFLVEHAPADKKGKYA
SWLQASMAISNIMGALVAFAVTSLLTEQQVGDWGWRIPFFIGVLIAPVGLWLRKTLDETPAFQAERASAGREPAAAPLRQ
VFSDYLGHLIKGFAFSILWAVCVYVLIIYMPIFVQRAFGFTPSQAFTASLIGNVFLAVGCVCAGTVSDRIGRRLMLGASA
ALMLVAVYPLISWLQASPTLTTLVIVQSLFCIMVSGFVGVAPATLSEVFPTHVRTTGMSLAYNAAVTIFGGFAPATLTWL
GSTGIGFHAPAFYVMAAAAASLIAVLVLPKPYSTNCGRGAVPAPADP

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=438, Percent_Identity=34.2465753424658, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=33.4928229665072, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788292, Length=439, Percent_Identity=28.0182232346241, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=30.4651162790698, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082231, Length=436, Percent_Identity=24.0825688073394, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082404, Length=419, Percent_Identity=24.3436754176611, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47012; Mature: 47012

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTRQDWRSVPAKRSSPGRAVAAASIGNALEWYDFTVYALFAIYIAQNFFPGGDDTVEL
CCCCHHHHHCCCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
VKAFLAFGLGFVIRPLGAVVIGVYGDKAGRKAALFLTIMIMAAGTLLIAIAPTYAAMGIG
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHCC
APLMILAGRVLQGFSAGGEIGGAAAFLVEHAPADKKGKYASWLQASMAISNIMGALVAFA
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VTSLLTEQQVGDWGWRIPFFIGVLIAPVGLWLRKTLDETPAFQAERASAGREPAAAPLRQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHH
VFSDYLGHLIKGFAFSILWAVCVYVLIIYMPIFVQRAFGFTPSQAFTASLIGNVFLAVGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VCAGTVSDRIGRRLMLGASAALMLVAVYPLISWLQASPTLTTLVIVQSLFCIMVSGFVGV
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
APATLSEVFPTHVRTTGMSLAYNAAVTIFGGFAPATLTWLGSTGIGFHAPAFYVMAAAAA
CHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
SLIAVLVLPKPYSTNCGRGAVPAPADP
HHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
METTRQDWRSVPAKRSSPGRAVAAASIGNALEWYDFTVYALFAIYIAQNFFPGGDDTVEL
CCCCHHHHHCCCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
VKAFLAFGLGFVIRPLGAVVIGVYGDKAGRKAALFLTIMIMAAGTLLIAIAPTYAAMGIG
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHCC
APLMILAGRVLQGFSAGGEIGGAAAFLVEHAPADKKGKYASWLQASMAISNIMGALVAFA
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VTSLLTEQQVGDWGWRIPFFIGVLIAPVGLWLRKTLDETPAFQAERASAGREPAAAPLRQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHH
VFSDYLGHLIKGFAFSILWAVCVYVLIIYMPIFVQRAFGFTPSQAFTASLIGNVFLAVGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VCAGTVSDRIGRRLMLGASAALMLVAVYPLISWLQASPTLTTLVIVQSLFCIMVSGFVGV
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
APATLSEVFPTHVRTTGMSLAYNAAVTIFGGFAPATLTWLGSTGIGFHAPAFYVMAAAAA
CHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHH
SLIAVLVLPKPYSTNCGRGAVPAPADP
HHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]