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The map label for this gene is kipI [H]

Identifier: 119386142

GI number: 119386142

Start: 572761

End: 573534

Strand: Direct

Name: kipI [H]

Synonym: Pden_3430

Alternate gene names: 119386142

Gene position: 572761-573534 (Clockwise)

Preceding gene: 119386141

Following gene: 119386143

Centisome position: 33.11

GC content: 67.7

Gene sequence:

>774_bases
ATGCCCGCGGCCCGCACGGGGCCGCGGCGCACGTCACCAGAACAAGAAGAAATGATGCAACCCAAGATCCTACCCTGCGG
CGATACCGCGCTTTCGCTGCAATTCGGCGAAACCATCGATGCTAAGGCCAATGCGCGGGTGATCGCCACCGCCGCGCGTC
TGGAGGGCATGGCGGTGCCGGGCCTTGTCGATATCGTGCCGACCTATCGCTCGCTCCTGCTGCGCTACGATCCCGAAATC
GTCAGCGGCGCCCGCCTCGAAACCATCCTGGCCGGACTGGCGCCGGATGATTGCGCAAGCGAGGCGCCGTCGCGGCTATG
GGTCGTGCCCTGCCTCTATGGCGGAGAGGTCGGCCAGGATCTTGACGAACTGGCCCGGATGAAGGGGATGCCGCCCGACG
AGATCGTCGCGCTGCACTCGGCGGCGGAATACCGCATCTACATGATCGGCTTCGCGCCGGGCTTCGCCTATCTGGGCGGC
CTGCCCGAGATCCTGCACACGCCGCGCCTGCCCAGGCCGCGCCAGAACATCCCGGCGGGCGCGATCGGCATCGGCGGGCA
GCAGGGCAATATCAACTCGGTTGCGGGGCCTTCGGGCTGGCGCTTCATCGGCTGGACGCCGCTGCGATCATTCGATCCGT
CGCGGACCGAGCCCTTCCTGTTCCGGGCTGGCGACCGCATCCGGTTTCGTCCCATCGATGCTGCCGAGGCCCAGGTCCTG
GCCGGCCGCGTCGCGGCAGGAGAGGCGATTCTCCAACCCGAGGCCATGGCATGA

Upstream 100 bases:

>100_bases
CCGTTCTTCTACATCATGTCGGCATTCGTGCTGCTGATCGCGGTCTTTCCGGGGATCATCGACCTGCTGCCCGGCATGAT
GGCGGGACGATAGGGCAGGG

Downstream 100 bases:

>100_bases
GCGTCGAGATCGTTTCCGCCGGGCCGATGCTGACCGTCCAGGACGCGGGCAGGATCGGGCTGCGGCATATGGGCGTCTCG
GATGCCGGTCCGGTCGATGC

Product: allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MPAARTGPRRTSPEQEEMMQPKILPCGDTALSLQFGETIDAKANARVIATAARLEGMAVPGLVDIVPTYRSLLLRYDPEI
VSGARLETILAGLAPDDCASEAPSRLWVVPCLYGGEVGQDLDELARMKGMPPDEIVALHSAAEYRIYMIGFAPGFAYLGG
LPEILHTPRLPRPRQNIPAGAIGIGGQQGNINSVAGPSGWRFIGWTPLRSFDPSRTEPFLFRAGDRIRFRPIDAAEAQVL
AGRVAAGEAILQPEAMA

Sequences:

>Translated_257_residues
MPAARTGPRRTSPEQEEMMQPKILPCGDTALSLQFGETIDAKANARVIATAARLEGMAVPGLVDIVPTYRSLLLRYDPEI
VSGARLETILAGLAPDDCASEAPSRLWVVPCLYGGEVGQDLDELARMKGMPPDEIVALHSAAEYRIYMIGFAPGFAYLGG
LPEILHTPRLPRPRQNIPAGAIGIGGQQGNINSVAGPSGWRFIGWTPLRSFDPSRTEPFLFRAGDRIRFRPIDAAEAQVL
AGRVAAGEAILQPEAMA
>Mature_256_residues
PAARTGPRRTSPEQEEMMQPKILPCGDTALSLQFGETIDAKANARVIATAARLEGMAVPGLVDIVPTYRSLLLRYDPEIV
SGARLETILAGLAPDDCASEAPSRLWVVPCLYGGEVGQDLDELARMKGMPPDEIVALHSAAEYRIYMIGFAPGFAYLGGL
PEILHTPRLPRPRQNIPAGAIGIGGQQGNINSVAGPSGWRFIGWTPLRSFDPSRTEPFLFRAGDRIRFRPIDAAEAQVLA
GRVAAGEAILQPEAMA

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=215, Percent_Identity=38.6046511627907, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 27612; Mature: 27481

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAARTGPRRTSPEQEEMMQPKILPCGDTALSLQFGETIDAKANARVIATAARLEGMAVP
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEHHHCCCCCC
GLVDIVPTYRSLLLRYDPEIVSGARLETILAGLAPDDCASEAPSRLWVVPCLYGGEVGQD
CHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCCCCHHHHCCCCEEEEEEEEECCCCCCC
LDELARMKGMPPDEIVALHSAAEYRIYMIGFAPGFAYLGGLPEILHTPRLPRPRQNIPAG
HHHHHHHCCCCHHHEEEEECCCCEEEEEEEECCHHHHHCCCHHHHHCCCCCCCCCCCCCC
AIGIGGQQGNINSVAGPSGWRFIGWTPLRSFDPSRTEPFLFRAGDRIRFRPIDAAEAQVL
EEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHHH
AGRVAAGEAILQPEAMA
HHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
PAARTGPRRTSPEQEEMMQPKILPCGDTALSLQFGETIDAKANARVIATAARLEGMAVP
CCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEHHHCCCCCC
GLVDIVPTYRSLLLRYDPEIVSGARLETILAGLAPDDCASEAPSRLWVVPCLYGGEVGQD
CHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHCCCCCHHHHCCCCEEEEEEEEECCCCCCC
LDELARMKGMPPDEIVALHSAAEYRIYMIGFAPGFAYLGGLPEILHTPRLPRPRQNIPAG
HHHHHHHCCCCHHHEEEEECCCCEEEEEEEECCHHHHHCCCHHHHHCCCCCCCCCCCCCC
AIGIGGQQGNINSVAGPSGWRFIGWTPLRSFDPSRTEPFLFRAGDRIRFRPIDAAEAQVL
EEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHHH
AGRVAAGEAILQPEAMA
HHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]