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The map label for this gene is pycB [H]

Identifier: 119357008

GI number: 119357008

Start: 1347508

End: 1349412

Strand: Direct

Name: pycB [H]

Synonym: Cpha266_1192

Alternate gene names: 119357008

Gene position: 1347508-1349412 (Clockwise)

Preceding gene: 119357007

Following gene: 119357010

Centisome position: 43.0

GC content: 49.66

Gene sequence:

>1905_bases
ATGAAAAAAATCAGGTTTATGGATGTTTCTTTTCGTGACGGGTTTCAGTCATGTTACGGAGCAAGGGTAAAAACGGAAGA
CTTTTTGCCAGCGCTTGAAGCTGCTGTTCATGCAGGAACCGATAGTTTTGAGATTGGCGGCGGAGCACGTTTTCAAAGTC
TTTATTTCTATTGTGAAGAAGACGCTTTCGACATGATGGATGCTGCCCGCAAGGTTGTTGGAGAGAATATCAATCTTCAG
ACCCTTTCAAGGGGCGCTAATGTCGTAGGTCTTGTGTCGCAGTCGCGGGATATTATCGATCTTCACGCGCGGCTTTTTAA
AAAACACGGCATAACGACGATACGCAATTTCGATGCCCTGATGGATGTTCGCAATCTTGCCTATTCCGGTCATTGCATTC
ACGACGCGGGTCTCAGGCATCAGGTGGTTATTGCCTTGATGGGTCTTCCTCCTGGACTTGGGGAAAATTATTGCCATACG
CCTGCATTTTATCTTGACAAGCTGCAGGAAATTCTTGATGCCGTCATTCCATACGACAGCGTCGCTTTCAAGGATGCATC
TGGAACAACAACACCCGCAGTTGTCTATGAGGTGATTAAAGGTGCCAGAAAAATGCTTCCGGCAGAAACGCTGATTGAAT
TCCATACCCACGATACGGCAGGAATGGGAGTCGCCTGTAACTTTGCAGCAATTGAGGGGGGAGCGGATATTATCGATCTG
TCGATGGCTCCGGTGAGCGGCGGCACTGCCGAAGTTGATATTCTTACCATGTGGCACTGTCTCAGAGGAACCTGTTACAC
GCTTGATATCGACCAGGAAAAGTATCTTGAGGTCGAGTCACTGTTTATGCGGCTGATGGATAAATATTACATGCCGCCTG
AAGCCAAGGAGGTCAATCCCGTCATTCCTTTTTCACCCATGCCGGGCGGAGCGCTTACCGCAAACACGCAGATGATGCGG
GATAACAATACGCTGCATCTTTTTCCTGAGGTTATAAAAAATATGCGTGAAGTTGTTGCTAAAGGAGGGTATGGATCATC
AGTGACTCCGGTATCGCAATTTTATTTTCAGCAGGCATTTTCCAATACGGTGCAGGGCGCGTGGAAAAATATTACAGACG
GGTATGGCAAAATGGTTCTCGGTTATTTCGGACGTACTCCGGCATTGCCTGACGAGGATGTTGTCAGGCTTGCTTCAGAG
CAGCTCGGGCTTGAGCCGGTGGTCGAGGATGTGCATGATATCAATGATCGCAATCCGGAACTCGGTATAGCATACAACAG
GGGTCTGCTTGAAACGGCTGCGCTTCCCGTTACCGATGAGAATATCTTTATTGCGGCAACCTGTGGCGCCAAGGGTATCG
CCTTTCTTAAAGGAGAGTGCACGTCTGGTATCCGTTACAAGGCGGATATTGATGCCGAGATGGCGGCCAAGCCAAAATCA
GAGGTTGTTGCTGCGTATAATGAGGTACACAAGCACGATATCCATCCGCATGGCGTCAACAAGCGGTTGGTCGATCTGTT
TGCAAAATCAGCATCAAAGGAACCCGTTGTTTCCTCAAAGGCGATCGCTCTTGCCGGTAATTTTACGATGTATATTGATG
GCGCTCCCGTTACCGTTACGTTTTCCGATGGAGCAGGCAGTGACCTGCCGGCAAAACCGGCAGCTCCGGCTATCGTTAAC
CAGGCGACCAAGGGAACGCCTGTTACGGCGGCTATGCCGGGAAACCTCCTCTCACTTGCGGTGCAAGTCGGTGAAGAGGT
AACCGAAGGATGCGAGGTTGCCGTTCTTGAAGCCATGAAGATGGAGACGCCGCTCAAGGCCCCTTGTTCAGGAACAATAC
TTTCCATTGCCGTTTCCGTCGGAGAAGCTGTCGGCATGGGAGAGGCTCTGATGTATATCGGATAG

Upstream 100 bases:

>100_bases
TTATCGGTATGAAAAGGCCTTGAAGGTTTGAATGCCGAGGATCGGATTGGGGAATTCTTTTTGCCAATTATTACCGTTTT
TTGAAAAAGGAGTGAGGCCT

Downstream 100 bases:

>100_bases
CATGCAGGCAGCATAAAAGGTAAAAAAGGTGGCGGCGATTTTCTGCCACCCTTTTTTTGCCTTTTATGAGCCGAATTCCA
TTATTGCGTCAGAAGAATTT

Product: biotin/lipoyl attachment domain-containing protein

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MKKIRFMDVSFRDGFQSCYGARVKTEDFLPALEAAVHAGTDSFEIGGGARFQSLYFYCEEDAFDMMDAARKVVGENINLQ
TLSRGANVVGLVSQSRDIIDLHARLFKKHGITTIRNFDALMDVRNLAYSGHCIHDAGLRHQVVIALMGLPPGLGENYCHT
PAFYLDKLQEILDAVIPYDSVAFKDASGTTTPAVVYEVIKGARKMLPAETLIEFHTHDTAGMGVACNFAAIEGGADIIDL
SMAPVSGGTAEVDILTMWHCLRGTCYTLDIDQEKYLEVESLFMRLMDKYYMPPEAKEVNPVIPFSPMPGGALTANTQMMR
DNNTLHLFPEVIKNMREVVAKGGYGSSVTPVSQFYFQQAFSNTVQGAWKNITDGYGKMVLGYFGRTPALPDEDVVRLASE
QLGLEPVVEDVHDINDRNPELGIAYNRGLLETAALPVTDENIFIAATCGAKGIAFLKGECTSGIRYKADIDAEMAAKPKS
EVVAAYNEVHKHDIHPHGVNKRLVDLFAKSASKEPVVSSKAIALAGNFTMYIDGAPVTVTFSDGAGSDLPAKPAAPAIVN
QATKGTPVTAAMPGNLLSLAVQVGEEVTEGCEVAVLEAMKMETPLKAPCSGTILSIAVSVGEAVGMGEALMYIG

Sequences:

>Translated_634_residues
MKKIRFMDVSFRDGFQSCYGARVKTEDFLPALEAAVHAGTDSFEIGGGARFQSLYFYCEEDAFDMMDAARKVVGENINLQ
TLSRGANVVGLVSQSRDIIDLHARLFKKHGITTIRNFDALMDVRNLAYSGHCIHDAGLRHQVVIALMGLPPGLGENYCHT
PAFYLDKLQEILDAVIPYDSVAFKDASGTTTPAVVYEVIKGARKMLPAETLIEFHTHDTAGMGVACNFAAIEGGADIIDL
SMAPVSGGTAEVDILTMWHCLRGTCYTLDIDQEKYLEVESLFMRLMDKYYMPPEAKEVNPVIPFSPMPGGALTANTQMMR
DNNTLHLFPEVIKNMREVVAKGGYGSSVTPVSQFYFQQAFSNTVQGAWKNITDGYGKMVLGYFGRTPALPDEDVVRLASE
QLGLEPVVEDVHDINDRNPELGIAYNRGLLETAALPVTDENIFIAATCGAKGIAFLKGECTSGIRYKADIDAEMAAKPKS
EVVAAYNEVHKHDIHPHGVNKRLVDLFAKSASKEPVVSSKAIALAGNFTMYIDGAPVTVTFSDGAGSDLPAKPAAPAIVN
QATKGTPVTAAMPGNLLSLAVQVGEEVTEGCEVAVLEAMKMETPLKAPCSGTILSIAVSVGEAVGMGEALMYIG
>Mature_634_residues
MKKIRFMDVSFRDGFQSCYGARVKTEDFLPALEAAVHAGTDSFEIGGGARFQSLYFYCEEDAFDMMDAARKVVGENINLQ
TLSRGANVVGLVSQSRDIIDLHARLFKKHGITTIRNFDALMDVRNLAYSGHCIHDAGLRHQVVIALMGLPPGLGENYCHT
PAFYLDKLQEILDAVIPYDSVAFKDASGTTTPAVVYEVIKGARKMLPAETLIEFHTHDTAGMGVACNFAAIEGGADIIDL
SMAPVSGGTAEVDILTMWHCLRGTCYTLDIDQEKYLEVESLFMRLMDKYYMPPEAKEVNPVIPFSPMPGGALTANTQMMR
DNNTLHLFPEVIKNMREVVAKGGYGSSVTPVSQFYFQQAFSNTVQGAWKNITDGYGKMVLGYFGRTPALPDEDVVRLASE
QLGLEPVVEDVHDINDRNPELGIAYNRGLLETAALPVTDENIFIAATCGAKGIAFLKGECTSGIRYKADIDAEMAAKPKS
EVVAAYNEVHKHDIHPHGVNKRLVDLFAKSASKEPVVSSKAIALAGNFTMYIDGAPVTVTFSDGAGSDLPAKPAAPAIVN
QATKGTPVTAAMPGNLLSLAVQVGEEVTEGCEVAVLEAMKMETPLKAPCSGTILSIAVSVGEAVGMGEALMYIG

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=276, Percent_Identity=26.4492753623188, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI106049295, Length=276, Percent_Identity=26.4492753623188, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI106049292, Length=276, Percent_Identity=26.4492753623188, Blast_Score=96, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562816, Length=357, Percent_Identity=29.1316526610644, Blast_Score=96, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321376, Length=315, Percent_Identity=30.1587301587302, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319695, Length=315, Percent_Identity=29.5238095238095, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI281363050, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652224, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652222, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652220, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652218, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652212, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652210, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652214, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19921944, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24652216, Length=359, Percent_Identity=27.8551532033426, Blast_Score=100, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 68350; Mature: 68350

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIRFMDVSFRDGFQSCYGARVKTEDFLPALEAAVHAGTDSFEIGGGARFQSLYFYCEE
CCCEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEECC
DAFDMMDAARKVVGENINLQTLSRGANVVGLVSQSRDIIDLHARLFKKHGITTIRNFDAL
CHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHH
MDVRNLAYSGHCIHDAGLRHQVVIALMGLPPGLGENYCHTPAFYLDKLQEILDAVIPYDS
HHHHHHHCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VAFKDASGTTTPAVVYEVIKGARKMLPAETLIEFHTHDTAGMGVACNFAAIEGGADIIDL
EEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEE
SMAPVSGGTAEVDILTMWHCLRGTCYTLDIDQEKYLEVESLFMRLMDKYYMPPEAKEVNP
EECCCCCCCCEEHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VIPFSPMPGGALTANTQMMRDNNTLHLFPEVIKNMREVVAKGGYGSSVTPVSQFYFQQAF
CCCCCCCCCCCEECCCEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SNTVQGAWKNITDGYGKMVLGYFGRTPALPDEDVVRLASEQLGLEPVVEDVHDINDRNPE
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
LGIAYNRGLLETAALPVTDENIFIAATCGAKGIAFLKGECTSGIRYKADIDAEMAAKPKS
EEEEECCCCHHHCCCCEECCCEEEEEECCCCCEEEEECCCCCCCEEEECCCHHHHCCCHH
EVVAAYNEVHKHDIHPHGVNKRLVDLFAKSASKEPVVSSKAIALAGNFTMYIDGAPVTVT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEECCCEEEEE
FSDGAGSDLPAKPAAPAIVNQATKGTPVTAAMPGNLLSLAVQVGEEVTEGCEVAVLEAMK
ECCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHH
METPLKAPCSGTILSIAVSVGEAVGMGEALMYIG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MKKIRFMDVSFRDGFQSCYGARVKTEDFLPALEAAVHAGTDSFEIGGGARFQSLYFYCEE
CCCEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEECC
DAFDMMDAARKVVGENINLQTLSRGANVVGLVSQSRDIIDLHARLFKKHGITTIRNFDAL
CHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHH
MDVRNLAYSGHCIHDAGLRHQVVIALMGLPPGLGENYCHTPAFYLDKLQEILDAVIPYDS
HHHHHHHCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VAFKDASGTTTPAVVYEVIKGARKMLPAETLIEFHTHDTAGMGVACNFAAIEGGADIIDL
EEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEE
SMAPVSGGTAEVDILTMWHCLRGTCYTLDIDQEKYLEVESLFMRLMDKYYMPPEAKEVNP
EECCCCCCCCEEHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VIPFSPMPGGALTANTQMMRDNNTLHLFPEVIKNMREVVAKGGYGSSVTPVSQFYFQQAF
CCCCCCCCCCCEECCCEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SNTVQGAWKNITDGYGKMVLGYFGRTPALPDEDVVRLASEQLGLEPVVEDVHDINDRNPE
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
LGIAYNRGLLETAALPVTDENIFIAATCGAKGIAFLKGECTSGIRYKADIDAEMAAKPKS
EEEEECCCCHHHCCCCEECCCEEEEEECCCCCEEEEECCCCCCCEEEECCCHHHHCCCHH
EVVAAYNEVHKHDIHPHGVNKRLVDLFAKSASKEPVVSSKAIALAGNFTMYIDGAPVTVT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEECCCEEEEE
FSDGAGSDLPAKPAAPAIVNQATKGTPVTAAMPGNLLSLAVQVGEEVTEGCEVAVLEAMK
ECCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHH
METPLKAPCSGTILSIAVSVGEAVGMGEALMYIG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]