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The map label for this gene is kojP [H]

Identifier: 119357010

GI number: 119357010

Start: 1350932

End: 1353295

Strand: Direct

Name: kojP [H]

Synonym: Cpha266_1194

Alternate gene names: 119357010

Gene position: 1350932-1353295 (Clockwise)

Preceding gene: 119357008

Following gene: 119357011

Centisome position: 43.11

GC content: 44.08

Gene sequence:

>2364_bases
ATGTCCCGACGTTCCGCTTCTGACCTTGCTTCCGCTTGCTTTTCGGCTGAAAAATTACTTGAACTCTCTGCTGAAGAGTG
GCTTCTGAGACGAAAGGTTTTCAGAAATTCCGAAAAAAGCATTCAGGCAAACGAGTCGATTTTCACCATTGGAAACGGTT
ATCTCAATGTCAGGGGAAGTCTTGAAGAGCTCCCTCCGGGTCATTGCGGCGGGATGTATATCAGCGGGATTTACGATAAA
TCAGAAGCAGATGTTGAAGAATTGGTGAAATGTCCCATGTGGACGGATGTGTCAGTCTGGAGCGAAGGAGAGAAATTCTG
TTTGTCTTGCTGCAAGATACTCTTTCATGAGCAGGTGCTCGATATGAAAAAAGGGGTGCTTCATCGTCTGTCGACCCTGA
AAAACGCGGCAGGAAAAATCATCACCATTCAGACAGCCCGACTTGTTTTCATGCACAATGTGCACCTGGGTTATCTGTTT
GTAAAAGTGACCCCCAGAAACTTTTCGGCACCGTTGAGAGTCTTGAGCGGTCTTAATGGTGATGTCTGCAACAGGGGTTA
TTTTCCGAGAGAGATGCTCAAGCATCTTCAGCTTGAAAAAATTGAGCGCGGAAGAGCTTTCATGTATCTCGAAATGAAAA
CACGTGATCGCGGTATAAGAATATCCGAAGCGGCATCATGGAAGCTGATTTCGCATGACGCCTCCGTGCAGCGGTGGGAG
CCCCGGATATACGGAGAGAAATTTACCAGTGAAATTACTATTGATGCTCAAAAAGGCGAATCCTATATTTTTGAAAAGCT
TTGCGTGGTGTTGACAAGCAGGGAAGTCCCCGATGCACAATTATACAAAGAGACTATCTGTCAATTGAAAGCCTATGTCC
GAAGCACCGCTCTTAACGAAATTGCAGCAAACTTTACTGTCTGGCAAGAAAAATGGAAGCAATCCGATGTCGTCATTGTC
GGCGATGAGGAGGCGCAGAAAGCGTTGCGCTTCAATATTTATCACCTGCTTATCAACGGGCCGCCGAGAGTTGGAAGCAT
AGGGGCAAAGTTTCTGAGTTCAGAAGGATATCTCGGACATGTTTTCTGGGATACCGAAATTTTTATTCTGCCTTTTTATA
TCTACAACTTTCCGGAGATCGCCCGGAACATGCTCTTATACCGTTACCATACACTTCCGGGAGCGGTAAAAAACGCCGTG
AATGCCGGTTGTACAGGAGCGAAATACGCATGGGAATCGGCAACAACCGGAGAGGATGTCACTCCTCGATTTGCATCAAA
ACTTGAAAAAACCATCCGGTTTATCTATACCGGTACAGAAGAGGACCATATTGTTTCTGACGTGATCTATGGTCTTGAAA
AGTATTTTCGTGTTACGGGTGACGAGAGTTTTCTTCTTGAATACGGACTTGAAATGCTTTTTATGACCGCCAGATTTTGG
GCAAGCCGGGTTGTCAAGGTAGATGATTCCTATGAGATTCATTGCGTCATAGGACCGGATGAGTTTCATGAGCATGTCAA
TAACAATGCCTACACAAATTTTATGGTGAAATGGCATTTGGAACTTGCCGCGACTCTCTACGGCTATATGCATAAAAAAA
ACCCGGTTTCACTTGACTATCTCTGCCGGAAAATTTGTTTCAACAAATCCGAAGCTGATAACTGGCTTGTTATCAGCAGG
AATATGAAATTTTCTGTTGATCCGCAAACCCGTCTTTTCGAGCAGTTCGATGGTTACTTCAACCTCAGAGATTATGTTTT
CAGTGGTTATGATCGAACAGGCCATCCGACTTTGCCCCGAGGTGTCAATTACAGAACGATCGGGTCGACAACTCTGATCA
AGCAGGCTGATGTGGTCATCATGATGTTGCTGTTCCCTCACAACTTTAGTGATGAGGAAAAGAGGGTAAATTACGAGTAT
TATGAGCAGCGAACAGCTCATAAGTCATCGTTAAGTCACTGTACTTACGCCATGATGGGACTTGCAATCGGAAAGCGGAC
AAGGGCATATAGTTATTTTATGAAAACGGCACTGTTTGATCTTGACAATCTTCATAAAAATACTCATTTGGGGATTCATG
CTGCAGCCGTGGGTGGAACATGGCAGACCGTTGTCAACGGGTTCGGGGGATTGTCGATTAAATCAGACCGTATTGTCATG
AACCCTTGGCTTCCAAAGAAATGGGAGAGTCTTTCATTTACCGTTCGATGGAAAGCGAGGTTCGTTGATATCAATATTTT
TCATGACAGAATATCGATATTGATCCGTTCAGAAGATGCTGTTGAAATTCCTTTGAGTCTTTACAGAAAAACATATAAAA
TAAAATCTAACGAAGTTCAGGTTCTTCACTACTGTTCATCCTGA

Upstream 100 bases:

>100_bases
AAGAACTATTAGTGTCGGATAATTGTAATGTTACGTGTTAAAATAGTTCTGCCTGTTGACTTTTTTTGTCTCACCGCTGT
TTATATCCAGGGAGAATTTC

Downstream 100 bases:

>100_bases
CACGAAGGCGGAGGGACGGTTGCGATGAGATTGATCGTTGTAGGCTGTTCAGGCCGCTCAAGGTATTATCTGGCGGATCG
CTTGAAAAGTTGTACTATGT

Product: Kojibiose phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 787; Mature: 786

Protein sequence:

>787_residues
MSRRSASDLASACFSAEKLLELSAEEWLLRRKVFRNSEKSIQANESIFTIGNGYLNVRGSLEELPPGHCGGMYISGIYDK
SEADVEELVKCPMWTDVSVWSEGEKFCLSCCKILFHEQVLDMKKGVLHRLSTLKNAAGKIITIQTARLVFMHNVHLGYLF
VKVTPRNFSAPLRVLSGLNGDVCNRGYFPREMLKHLQLEKIERGRAFMYLEMKTRDRGIRISEAASWKLISHDASVQRWE
PRIYGEKFTSEITIDAQKGESYIFEKLCVVLTSREVPDAQLYKETICQLKAYVRSTALNEIAANFTVWQEKWKQSDVVIV
GDEEAQKALRFNIYHLLINGPPRVGSIGAKFLSSEGYLGHVFWDTEIFILPFYIYNFPEIARNMLLYRYHTLPGAVKNAV
NAGCTGAKYAWESATTGEDVTPRFASKLEKTIRFIYTGTEEDHIVSDVIYGLEKYFRVTGDESFLLEYGLEMLFMTARFW
ASRVVKVDDSYEIHCVIGPDEFHEHVNNNAYTNFMVKWHLELAATLYGYMHKKNPVSLDYLCRKICFNKSEADNWLVISR
NMKFSVDPQTRLFEQFDGYFNLRDYVFSGYDRTGHPTLPRGVNYRTIGSTTLIKQADVVIMMLLFPHNFSDEEKRVNYEY
YEQRTAHKSSLSHCTYAMMGLAIGKRTRAYSYFMKTALFDLDNLHKNTHLGIHAAAVGGTWQTVVNGFGGLSIKSDRIVM
NPWLPKKWESLSFTVRWKARFVDINIFHDRISILIRSEDAVEIPLSLYRKTYKIKSNEVQVLHYCSS

Sequences:

>Translated_787_residues
MSRRSASDLASACFSAEKLLELSAEEWLLRRKVFRNSEKSIQANESIFTIGNGYLNVRGSLEELPPGHCGGMYISGIYDK
SEADVEELVKCPMWTDVSVWSEGEKFCLSCCKILFHEQVLDMKKGVLHRLSTLKNAAGKIITIQTARLVFMHNVHLGYLF
VKVTPRNFSAPLRVLSGLNGDVCNRGYFPREMLKHLQLEKIERGRAFMYLEMKTRDRGIRISEAASWKLISHDASVQRWE
PRIYGEKFTSEITIDAQKGESYIFEKLCVVLTSREVPDAQLYKETICQLKAYVRSTALNEIAANFTVWQEKWKQSDVVIV
GDEEAQKALRFNIYHLLINGPPRVGSIGAKFLSSEGYLGHVFWDTEIFILPFYIYNFPEIARNMLLYRYHTLPGAVKNAV
NAGCTGAKYAWESATTGEDVTPRFASKLEKTIRFIYTGTEEDHIVSDVIYGLEKYFRVTGDESFLLEYGLEMLFMTARFW
ASRVVKVDDSYEIHCVIGPDEFHEHVNNNAYTNFMVKWHLELAATLYGYMHKKNPVSLDYLCRKICFNKSEADNWLVISR
NMKFSVDPQTRLFEQFDGYFNLRDYVFSGYDRTGHPTLPRGVNYRTIGSTTLIKQADVVIMMLLFPHNFSDEEKRVNYEY
YEQRTAHKSSLSHCTYAMMGLAIGKRTRAYSYFMKTALFDLDNLHKNTHLGIHAAAVGGTWQTVVNGFGGLSIKSDRIVM
NPWLPKKWESLSFTVRWKARFVDINIFHDRISILIRSEDAVEIPLSLYRKTYKIKSNEVQVLHYCSS
>Mature_786_residues
SRRSASDLASACFSAEKLLELSAEEWLLRRKVFRNSEKSIQANESIFTIGNGYLNVRGSLEELPPGHCGGMYISGIYDKS
EADVEELVKCPMWTDVSVWSEGEKFCLSCCKILFHEQVLDMKKGVLHRLSTLKNAAGKIITIQTARLVFMHNVHLGYLFV
KVTPRNFSAPLRVLSGLNGDVCNRGYFPREMLKHLQLEKIERGRAFMYLEMKTRDRGIRISEAASWKLISHDASVQRWEP
RIYGEKFTSEITIDAQKGESYIFEKLCVVLTSREVPDAQLYKETICQLKAYVRSTALNEIAANFTVWQEKWKQSDVVIVG
DEEAQKALRFNIYHLLINGPPRVGSIGAKFLSSEGYLGHVFWDTEIFILPFYIYNFPEIARNMLLYRYHTLPGAVKNAVN
AGCTGAKYAWESATTGEDVTPRFASKLEKTIRFIYTGTEEDHIVSDVIYGLEKYFRVTGDESFLLEYGLEMLFMTARFWA
SRVVKVDDSYEIHCVIGPDEFHEHVNNNAYTNFMVKWHLELAATLYGYMHKKNPVSLDYLCRKICFNKSEADNWLVISRN
MKFSVDPQTRLFEQFDGYFNLRDYVFSGYDRTGHPTLPRGVNYRTIGSTTLIKQADVVIMMLLFPHNFSDEEKRVNYEYY
EQRTAHKSSLSHCTYAMMGLAIGKRTRAYSYFMKTALFDLDNLHKNTHLGIHAAAVGGTWQTVVNGFGGLSIKSDRIVMN
PWLPKKWESLSFTVRWKARFVDINIFHDRISILIRSEDAVEIPLSLYRKTYKIKSNEVQVLHYCSS

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=440, Percent_Identity=27.7272727272727, Blast_Score=152, Evalue=9e-37,
Organism=Escherichia coli, GI1787575, Length=731, Percent_Identity=30.3693570451436, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6325283, Length=397, Percent_Identity=24.4332493702771, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583760, Length=379, Percent_Identity=26.6490765171504, Blast_Score=100, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 90613; Mature: 90482

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRSASDLASACFSAEKLLELSAEEWLLRRKVFRNSEKSIQANESIFTIGNGYLNVRGS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEECCCEEEECCC
LEELPPGHCGGMYISGIYDKSEADVEELVKCPMWTDVSVWSEGEKFCLSCCKILFHEQVL
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHH
DMKKGVLHRLSTLKNAAGKIITIQTARLVFMHNVHLGYLFVKVTPRNFSAPLRVLSGLNG
HHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECEEEEEEEEECCCCCCCHHHHHCCCCC
DVCNRGYFPREMLKHLQLEKIERGRAFMYLEMKTRDRGIRISEAASWKLISHDASVQRWE
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECCCCEEEEECCCCCHHCC
PRIYGEKFTSEITIDAQKGESYIFEKLCVVLTSREVPDAQLYKETICQLKAYVRSTALNE
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAANFTVWQEKWKQSDVVIVGDEEAQKALRFNIYHLLINGPPRVGSIGAKFLSSEGYLGH
HHHHHHHHHHHHCCCCEEEECCHHHHHHHHEEEEEEEECCCCCCCHHHHHHHCCCCCEEE
VFWDTEIFILPFYIYNFPEIARNMLLYRYHTLPGAVKNAVNAGCTGAKYAWESATTGEDV
EEECCEEEEEEHHHCCCHHHHHHHHHHHEECCCHHHHHHHCCCCCCCCEECCCCCCCCCC
TPRFASKLEKTIRFIYTGTEEDHIVSDVIYGLEKYFRVTGDESFLLEYGLEMLFMTARFW
CHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHH
ASRVVKVDDSYEIHCVIGPDEFHEHVNNNAYTNFMVKWHLELAATLYGYMHKKNPVSLDY
HHEEEEECCCEEEEEEECCHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCHHH
LCRKICFNKSEADNWLVISRNMKFSVDPQTRLFEQFDGYFNLRDYVFSGYDRTGHPTLPR
HHHHHHCCCCCCCCEEEEECCCEEECCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCC
GVNYRTIGSTTLIKQADVVIMMLLFPHNFSDEEKRVNYEYYEQRTAHKSSLSHCTYAMMG
CCCEEECCCCHHHHHHHHEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAIGKRTRAYSYFMKTALFDLDNLHKNTHLGIHAAAVGGTWQTVVNGFGGLSIKSDRIVM
HHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCEEECCEEEE
NPWLPKKWESLSFTVRWKARFVDINIFHDRISILIRSEDAVEIPLSLYRKTYKIKSNEVQ
CCCCCCCCCCCEEEEEEEEEEEEEEEEEEEEEEEEECCCCEECCHHHHHHHHEECCCCEE
VLHYCSS
EEEEECC
>Mature Secondary Structure 
SRRSASDLASACFSAEKLLELSAEEWLLRRKVFRNSEKSIQANESIFTIGNGYLNVRGS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEECCCEEEECCC
LEELPPGHCGGMYISGIYDKSEADVEELVKCPMWTDVSVWSEGEKFCLSCCKILFHEQVL
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHH
DMKKGVLHRLSTLKNAAGKIITIQTARLVFMHNVHLGYLFVKVTPRNFSAPLRVLSGLNG
HHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECEEEEEEEEECCCCCCCHHHHHCCCCC
DVCNRGYFPREMLKHLQLEKIERGRAFMYLEMKTRDRGIRISEAASWKLISHDASVQRWE
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEECCCCEEEEECCCCCHHCC
PRIYGEKFTSEITIDAQKGESYIFEKLCVVLTSREVPDAQLYKETICQLKAYVRSTALNE
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAANFTVWQEKWKQSDVVIVGDEEAQKALRFNIYHLLINGPPRVGSIGAKFLSSEGYLGH
HHHHHHHHHHHHCCCCEEEECCHHHHHHHHEEEEEEEECCCCCCCHHHHHHHCCCCCEEE
VFWDTEIFILPFYIYNFPEIARNMLLYRYHTLPGAVKNAVNAGCTGAKYAWESATTGEDV
EEECCEEEEEEHHHCCCHHHHHHHHHHHEECCCHHHHHHHCCCCCCCCEECCCCCCCCCC
TPRFASKLEKTIRFIYTGTEEDHIVSDVIYGLEKYFRVTGDESFLLEYGLEMLFMTARFW
CHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHH
ASRVVKVDDSYEIHCVIGPDEFHEHVNNNAYTNFMVKWHLELAATLYGYMHKKNPVSLDY
HHEEEEECCCEEEEEEECCHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCHHH
LCRKICFNKSEADNWLVISRNMKFSVDPQTRLFEQFDGYFNLRDYVFSGYDRTGHPTLPR
HHHHHHCCCCCCCCEEEEECCCEEECCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCC
GVNYRTIGSTTLIKQADVVIMMLLFPHNFSDEEKRVNYEYYEQRTAHKSSLSHCTYAMMG
CCCEEECCCCHHHHHHHHEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LAIGKRTRAYSYFMKTALFDLDNLHKNTHLGIHAAAVGGTWQTVVNGFGGLSIKSDRIVM
HHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCEEECCEEEE
NPWLPKKWESLSFTVRWKARFVDINIFHDRISILIRSEDAVEIPLSLYRKTYKIKSNEVQ
CCCCCCCCCCCEEEEEEEEEEEEEEEEEEEEEEEEECCCCEECCHHHHHHHHEECCCCEE
VLHYCSS
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA