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The map label for this gene is hyfI [H]

Identifier: 118578783

GI number: 118578783

Start: 342684

End: 343442

Strand: Direct

Name: hyfI [H]

Synonym: Ppro_0341

Alternate gene names: 118578783

Gene position: 342684-343442 (Clockwise)

Preceding gene: 118578782

Following gene: 118578784

Centisome position: 8.55

GC content: 67.85

Gene sequence:

>759_bases
ATGCTTCGAGCCATTCTCGCACGTTGCCGCCAGGGGCATCGCACGTTCGCCTATCCCGATGCCCCCTTGCCGTTGCCACC
CCGTTTTCGCGGGCTGCCGCTGCTGGACGCGTCCCGCTGCGAATCCGGCTGCCGTGCCTGCGCCGAGAGCTGCCCCTACG
GCGCCATTTCCGCTGACGGGGCGCTGAGTCTGGACATGGGGAAGTGCCTCTTCTGCGGCCAGTGCGCCGAGGAGTGCCGG
CAGGGCGCAATCTCCTTCAGCAACGATGCCGCTCTGGCCAGCCGCAGTCGGGAGGGGCTCGTGGTCAGGGCCGCCGAGCA
GTACCAGCCCGCCGAGGCCATGGGCAGAGAGCTTTTGCGCCTGTACGGCCGTTCGCTCAAGTTGCGCGAGGTGAGCGCCG
GCGGCTGCAACGCCTGTGAGGCGGACACCAACGTGCTCTCCACCATCGGCTGGGACCTGGGGCGCTTCGGCATCCAGTTC
GTGGCCAGCCCCCGCCATGCCGACGGCCTGTTCATAACCGGTCCGGTGAGCGAAAACATGCGTCTTGCCCTGCTGGATAC
CTATGCCGCCATACCCGACCCCAAGCTGGTCATCGTGACCGGCGCCTGCGCCATCAACGGCGGACCCTTCAAGGACCACG
ACGAGGTGTGCAATGGCGCCGATGGCCTGCTAGCGGTGGACCTGTACATCCCCGGCTGCCCCCCCAGCCCGCTGACAATC
CTGGACGGGCTGCTGCGGCTGATACGCCGCATCCGCTGA

Upstream 100 bases:

>100_bases
CTGTGACAGATGCAGTGACTGGAAACCGGTGACTCGTGACTCGTGACTGGTTGCGAGTCACGGCTTTTCCCTGAGTGAAA
CCTTCTGAATTGGAGTCAGT

Downstream 100 bases:

>100_bases
ACCCGCCGCGGGCCAGTTCCCCCTTTCTCTTGCGCGCACATTTCAGCTGCCAGGGAGAGGGGGTTTCTTTTGACTTTTTC
ATAAAGCGCTGATAGCATCG

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLRAILARCRQGHRTFAYPDAPLPLPPRFRGLPLLDASRCESGCRACAESCPYGAISADGALSLDMGKCLFCGQCAEECR
QGAISFSNDAALASRSREGLVVRAAEQYQPAEAMGRELLRLYGRSLKLREVSAGGCNACEADTNVLSTIGWDLGRFGIQF
VASPRHADGLFITGPVSENMRLALLDTYAAIPDPKLVIVTGACAINGGPFKDHDEVCNGADGLLAVDLYIPGCPPSPLTI
LDGLLRLIRRIR

Sequences:

>Translated_252_residues
MLRAILARCRQGHRTFAYPDAPLPLPPRFRGLPLLDASRCESGCRACAESCPYGAISADGALSLDMGKCLFCGQCAEECR
QGAISFSNDAALASRSREGLVVRAAEQYQPAEAMGRELLRLYGRSLKLREVSAGGCNACEADTNVLSTIGWDLGRFGIQF
VASPRHADGLFITGPVSENMRLALLDTYAAIPDPKLVIVTGACAINGGPFKDHDEVCNGADGLLAVDLYIPGCPPSPLTI
LDGLLRLIRRIR
>Mature_252_residues
MLRAILARCRQGHRTFAYPDAPLPLPPRFRGLPLLDASRCESGCRACAESCPYGAISADGALSLDMGKCLFCGQCAEECR
QGAISFSNDAALASRSREGLVVRAAEQYQPAEAMGRELLRLYGRSLKLREVSAGGCNACEADTNVLSTIGWDLGRFGIQF
VASPRHADGLFITGPVSENMRLALLDTYAAIPDPKLVIVTGACAINGGPFKDHDEVCNGADGLLAVDLYIPGCPPSPLTI
LDGLLRLIRRIR

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=118, Percent_Identity=36.4406779661017, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1788834, Length=136, Percent_Identity=41.9117647058824, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1789074, Length=132, Percent_Identity=43.9393939393939, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788624, Length=118, Percent_Identity=36.4406779661017, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789075, Length=111, Percent_Identity=30.6306306306306, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17509685, Length=118, Percent_Identity=37.2881355932203, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI18859983, Length=118, Percent_Identity=33.8983050847458, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24642371, Length=118, Percent_Identity=33.8983050847458, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24651058, Length=131, Percent_Identity=32.824427480916, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26953; Mature: 26953

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAILARCRQGHRTFAYPDAPLPLPPRFRGLPLLDASRCESGCRACAESCPYGAISADG
CHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
ALSLDMGKCLFCGQCAEECRQGAISFSNDAALASRSREGLVVRAAEQYQPAEAMGRELLR
EEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEHHHCCCHHHHHHHHHH
LYGRSLKLREVSAGGCNACEADTNVLSTIGWDLGRFGIQFVASPRHADGLFITGPVSENM
HHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCCC
RLALLDTYAAIPDPKLVIVTGACAINGGPFKDHDEVCNGADGLLAVDLYIPGCPPSPLTI
EEEEEEHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHCCCCCEEEEEEEECCCCCCHHHH
LDGLLRLIRRIR
HHHHHHHHHHCC
>Mature Secondary Structure
MLRAILARCRQGHRTFAYPDAPLPLPPRFRGLPLLDASRCESGCRACAESCPYGAISADG
CHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
ALSLDMGKCLFCGQCAEECRQGAISFSNDAALASRSREGLVVRAAEQYQPAEAMGRELLR
EEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEHHHCCCHHHHHHHHHH
LYGRSLKLREVSAGGCNACEADTNVLSTIGWDLGRFGIQFVASPRHADGLFITGPVSENM
HHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCCC
RLALLDTYAAIPDPKLVIVTGACAINGGPFKDHDEVCNGADGLLAVDLYIPGCPPSPLTI
EEEEEEHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHCCCCCEEEEEEEECCCCCCHHHH
LDGLLRLIRRIR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]