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The map label for this gene is cooH [H]

Identifier: 118578782

GI number: 118578782

Start: 341075

End: 342589

Strand: Direct

Name: cooH [H]

Synonym: Ppro_0340

Alternate gene names: 118578782

Gene position: 341075-342589 (Clockwise)

Preceding gene: 118578781

Following gene: 118578783

Centisome position: 8.51

GC content: 66.8

Gene sequence:

>1515_bases
ATGAGTCACGGCATGAAGACCTTCCACAACGGCGCCTGCTTCGGGCGCGGCGAGATCCCCCTGCTGGACAACGACGGCTT
CTTCCAGGGAATCCTGGACGCCACCGGGCACGGCTGGCGGATCTGCTCCTATTTCGGCGCTCCCTCTTCCTCAGGGGCGA
TCCTCTACTGCGTCATGGCCTCCAGGGGAAGCGGCTCCCTGGGTATCTTCTCCACCCGGGTAAAGGACAGCTTCCCCTCC
CTGGCCGAGGCCTGTCCCCAGCTGCACCTCTTCGAACGCGAGATCGCCGAGCAGTGCCTGGTGCGGCCGGAGGGGCACCC
CTGGTTCAAGCCGGTGCGCTTTCAGAAGCCGCTCCGCCCTGGGGAGCAGTTCTGGAAGAACAGTGACACCGGCCTGCTGC
CGGCGGTGATGGACTTCTACCGCGTGGAGGGGGACGAGGTCCACGAGGTAGCGGTGGGGCCGGTGCATGCCGGCATCATC
GAGCCGGGGCACTTCCGCTTCCAGTGCCATGGCGAAGAGGTCTTTCACCTGGAGATCGCCTTGGGGTTCCAGCACCGCGG
CATCGAGCGGGCCCTGATCGGCGGGCCGTCGCCGCGAACCATGCACCAGATGGAGGCGGTTGCCGGCGACACCAGCATCG
GCCATGGCCAGGCCTTTTGCATGAACATGGAGGCACTGGGCGCGGTTTTCGCGCCTCCCCGGGCCGAGGCGGTGCGCGGC
GTGGCCCTGGAACTGGAGCGCCTGGCCAACCACACCGGCGATCTGGGGGCCATTGCCGGTGATGTGGGTTATCTGCCCAC
CTCCTCGTTCTGCGGACGCATCCGGGGCGATTTCCTCAACATGACCGCCCTGTTGTGCGGCAGCCGCTTCGGACGCGGCC
TGCTCCGCCCCGGTGGGCTCGGCTATGACTGTGACGCCGAAATCGCACAGAGCGTGCTGGAGCGGTTACACATCGCGCGT
CGGGAGGTGACCCAGGCCATCAATCTGCTCTGGGACACCCCCTCGGTCATGGCCCGCCTGGAAAACACCGGCCAGCTTAG
CGCTGAGGTTGCCCGGGAGATCGGGCTGGTGGGGCCGGCCGCCCGCGCCTGCGACGTGAACCGCGACGTGCGCCGCGACC
ACCCCTTCGGCATCTTCCGCATGAGCCAGATTCCGGTCGTCACCGCCGCCAGCGGCGACGTGTACGGCCGCGCCCTGGTG
CGCTGGCTGGAGATGCAGCGCTCCCTGGATTTTATCGAGGAACAGCTGCAGCATCTCCCCCGTGGCGGCTTTCGCAACCA
GCCAACCAAGGTCGCGCCCGACCGCTGCGCCGTGGCGCTGACCGAGGGGTGGCGGGGAGAGATCTGCCACACCGCCCTGA
CCGACGGAGAGGGACGTTTCCAGCGCTACAAGATCACCGATCCCTCCTTCCACAACTGGACCGGTCTGGCCCTGGCGCTG
CGCAACCAGCAGATATCGGATTTCCCGCTCTGCAACAAGAGCTTTAACCTGTCCTACTGCGGGTTTGACCTGTGA

Upstream 100 bases:

>100_bases
CCCTGGTCATGCTGCTTGTTGTGTTTCTGCTGGGGGTCTGGATACCTGAGCCGCTGCTGGCTATCCTGCGCGCGGCTGCG
TCGCTTCTGGAGGTGCGGGC

Downstream 100 bases:

>100_bases
CAGATGCAGTGACTGGAAACCGGTGACTCGTGACTCGTGACTGGTTGCGAGTCACGGCTTTTCCCTGAGTGAAACCTTCT
GAATTGGAGTCAGTATGCTT

Product: NADH-ubiquinone oxidoreductase subunit 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSHGMKTFHNGACFGRGEIPLLDNDGFFQGILDATGHGWRICSYFGAPSSSGAILYCVMASRGSGSLGIFSTRVKDSFPS
LAEACPQLHLFEREIAEQCLVRPEGHPWFKPVRFQKPLRPGEQFWKNSDTGLLPAVMDFYRVEGDEVHEVAVGPVHAGII
EPGHFRFQCHGEEVFHLEIALGFQHRGIERALIGGPSPRTMHQMEAVAGDTSIGHGQAFCMNMEALGAVFAPPRAEAVRG
VALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTALLCGSRFGRGLLRPGGLGYDCDAEIAQSVLERLHIAR
REVTQAINLLWDTPSVMARLENTGQLSAEVAREIGLVGPAARACDVNRDVRRDHPFGIFRMSQIPVVTAASGDVYGRALV
RWLEMQRSLDFIEEQLQHLPRGGFRNQPTKVAPDRCAVALTEGWRGEICHTALTDGEGRFQRYKITDPSFHNWTGLALAL
RNQQISDFPLCNKSFNLSYCGFDL

Sequences:

>Translated_504_residues
MSHGMKTFHNGACFGRGEIPLLDNDGFFQGILDATGHGWRICSYFGAPSSSGAILYCVMASRGSGSLGIFSTRVKDSFPS
LAEACPQLHLFEREIAEQCLVRPEGHPWFKPVRFQKPLRPGEQFWKNSDTGLLPAVMDFYRVEGDEVHEVAVGPVHAGII
EPGHFRFQCHGEEVFHLEIALGFQHRGIERALIGGPSPRTMHQMEAVAGDTSIGHGQAFCMNMEALGAVFAPPRAEAVRG
VALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTALLCGSRFGRGLLRPGGLGYDCDAEIAQSVLERLHIAR
REVTQAINLLWDTPSVMARLENTGQLSAEVAREIGLVGPAARACDVNRDVRRDHPFGIFRMSQIPVVTAASGDVYGRALV
RWLEMQRSLDFIEEQLQHLPRGGFRNQPTKVAPDRCAVALTEGWRGEICHTALTDGEGRFQRYKITDPSFHNWTGLALAL
RNQQISDFPLCNKSFNLSYCGFDL
>Mature_503_residues
SHGMKTFHNGACFGRGEIPLLDNDGFFQGILDATGHGWRICSYFGAPSSSGAILYCVMASRGSGSLGIFSTRVKDSFPSL
AEACPQLHLFEREIAEQCLVRPEGHPWFKPVRFQKPLRPGEQFWKNSDTGLLPAVMDFYRVEGDEVHEVAVGPVHAGIIE
PGHFRFQCHGEEVFHLEIALGFQHRGIERALIGGPSPRTMHQMEAVAGDTSIGHGQAFCMNMEALGAVFAPPRAEAVRGV
ALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTALLCGSRFGRGLLRPGGLGYDCDAEIAQSVLERLHIARR
EVTQAINLLWDTPSVMARLENTGQLSAEVAREIGLVGPAARACDVNRDVRRDHPFGIFRMSQIPVVTAASGDVYGRALVR
WLEMQRSLDFIEEQLQHLPRGGFRNQPTKVAPDRCAVALTEGWRGEICHTALTDGEGRFQRYKITDPSFHNWTGLALALR
NQQISDFPLCNKSFNLSYCGFDL

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=367, Percent_Identity=23.1607629427793, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI260898743, Length=367, Percent_Identity=23.1607629427793, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789076, Length=464, Percent_Identity=27.3706896551724, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788832, Length=466, Percent_Identity=25.9656652360515, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI145693162, Length=517, Percent_Identity=20.6963249516441, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17555284, Length=285, Percent_Identity=23.859649122807, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17568379, Length=285, Percent_Identity=23.1578947368421, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221459469, Length=348, Percent_Identity=23.8505747126437, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24638644, Length=369, Percent_Identity=24.390243902439, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 55450; Mature: 55319

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHGMKTFHNGACFGRGEIPLLDNDGFFQGILDATGHGWRICSYFGAPSSSGAILYCVMA
CCCCCCHHCCCCEECCCCCEEECCCCHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEE
SRGSGSLGIFSTRVKDSFPSLAEACPQLHLFEREIAEQCLVRPEGHPWFKPVRFQKPLRP
CCCCCCEEEEEHHHHHCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GEQFWKNSDTGLLPAVMDFYRVEGDEVHEVAVGPVHAGIIEPGHFRFQCHGEEVFHLEIA
HHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCEEEEECCCEEEEEEEE
LGFQHRGIERALIGGPSPRTMHQMEAVAGDTSIGHGQAFCMNMEALGAVFAPPRAEAVRG
ECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHCCCCHHHHHHH
VALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTALLCGSRFGRGLLRPGGL
HHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GYDCDAEIAQSVLERLHIARREVTQAINLLWDTPSVMARLENTGQLSAEVAREIGLVGPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCC
ARACDVNRDVRRDHPFGIFRMSQIPVVTAASGDVYGRALVRWLEMQRSLDFIEEQLQHLP
HHHCCCCCCHHCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGGFRNQPTKVAPDRCAVALTEGWRGEICHTALTDGEGRFQRYKITDPSFHNWTGLALAL
CCCCCCCCCCCCCCCEEEEECCCCCCCEEHHEECCCCCCEEEEEECCCCCCCCCEEEEEE
RNQQISDFPLCNKSFNLSYCGFDL
ECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SHGMKTFHNGACFGRGEIPLLDNDGFFQGILDATGHGWRICSYFGAPSSSGAILYCVMA
CCCCCHHCCCCEECCCCCEEECCCCHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEE
SRGSGSLGIFSTRVKDSFPSLAEACPQLHLFEREIAEQCLVRPEGHPWFKPVRFQKPLRP
CCCCCCEEEEEHHHHHCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GEQFWKNSDTGLLPAVMDFYRVEGDEVHEVAVGPVHAGIIEPGHFRFQCHGEEVFHLEIA
HHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCEEEEECCCEEEEEEEE
LGFQHRGIERALIGGPSPRTMHQMEAVAGDTSIGHGQAFCMNMEALGAVFAPPRAEAVRG
ECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHCCCCHHHHHHH
VALELERLANHTGDLGAIAGDVGYLPTSSFCGRIRGDFLNMTALLCGSRFGRGLLRPGGL
HHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GYDCDAEIAQSVLERLHIARREVTQAINLLWDTPSVMARLENTGQLSAEVAREIGLVGPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCC
ARACDVNRDVRRDHPFGIFRMSQIPVVTAASGDVYGRALVRWLEMQRSLDFIEEQLQHLP
HHHCCCCCCHHCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGGFRNQPTKVAPDRCAVALTEGWRGEICHTALTDGEGRFQRYKITDPSFHNWTGLALAL
CCCCCCCCCCCCCCCEEEEECCCCCCCEEHHEECCCCCCEEEEEECCCCCCCCCEEEEEE
RNQQISDFPLCNKSFNLSYCGFDL
ECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]