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The map label for this gene is hyfF [H]

Identifier: 118578781

GI number: 118578781

Start: 339645

End: 341078

Strand: Direct

Name: hyfF [H]

Synonym: Ppro_0339

Alternate gene names: 118578781

Gene position: 339645-341078 (Clockwise)

Preceding gene: 118578780

Following gene: 118578782

Centisome position: 8.47

GC content: 65.27

Gene sequence:

>1434_bases
ATGCTCGCCGCTCTGATACTGCTACCGCTCGCCGGCGCCCTGCTGGCCTGGCTGCTCCCCGGCGACCGTTTTCGCCCGGC
GCTTTTGCCCCTGTTCGCCTTCCCCCACCTGGGGCTGACCCTGTGGCTTCTGTACCGTACCCCACTCCCTTCTCCGGGCG
GGTGGTTCAACCTGGACCCCCTGGGCAAGATCGTTCTGCTCTGCACCAGCGTGCTGTTCCTGTTCTGTTCCCTCTACGCC
CTGGGCTACCTGGCCTACCGCAGGGAGCGCTCCAGCCGCATCCTCTGCATCGGCCTGCTGGTTTGCCTGTCCGCCGCCTC
CCTGGTGACGACAACCCATCACCTGGGGCTGCTCTGGGTGGCGTTGGAGACCACCACCATCTCCATGGCGCCCCTGATCT
ACTTCAACCGCAACGCCCGCTCCATCGAAGCCACCTGGAAATATCTGCTGATCTGCTCCGTGGGGGTCGCCCTGGCCCTG
ATCGGCCTCTTCTTCCTGGCTTATTCCACCATCGTTTCCGGCATGGAGCCGTCGCTTCTGCTGGACGACCTGATGCGCGA
CGCGCCCCGGCTCTCCCCTAGCTGGGTACATGCCGCCTTCGTGTTCCTGCTGGTCGGTTTCGGCTCCAAGATGGGGCTGG
CGCCGCTGCACACCTGGAAGCCGGACGCCTATGGCGAGGCCCCTGGGTTGGTGGGCGCCATGCTGGCCGGCGGCCTGGTC
AACCTGGCCTTTCTGGCGCTCTTGCGCATCTACCAGCTCTGCCTGGCCACCCGGGAGATCGTCTTCTTCCAGAACGCCCT
GGTGGCCATGGGGCTGATTTCCATGTTCCTGGCCGCGGTATTCATGGCCCGCCAGGCAGACTTCAAGCGGATGCTGGCCT
ATTCCAGCGTGGAGCATGTGGGCATCATGGCCGTGGCCCTGGGGCTGGGGGGCAAGGCCATCTTCGGCGCACTGTTTCAT
GTCTTTGCCAACGGCCTGACCAAGGGGGTGCTGTTCCTCTCCTCGGGGAACATCCACCGCTCCTACAACAGCAAGAGCAC
CAGCCAGGTGGCGGGCGCCCTGCGGCGGCTCCCCTGGTCCGGGGGGCTGTTCCTGGCCGGCTTCATCGCCATCACCGGTT
CTCCTCCCTTTGCCCCCTTCATCAGCGAATTCACCATCGTCAGCAGCGCCTTCATGCAGGGGCGCATGCTGGTGGCGGCG
CTCTTCCTGCTCTCCCTGACCATCATCTTCATCGGCATGGCCCTGACCGTGCTCCCCATGGTCATGGGTGAGGTGCCCGA
GTCCAGTGAGTCAAGCCCCTACCGCGACACGGTCTGGACGGTGGGGCCGCCCCTGGTCATGCTGCTTGTTGTGTTTCTGC
TGGGGGTCTGGATACCTGAGCCGCTGCTGGCTATCCTGCGCGCGGCTGCGTCGCTTCTGGAGGTGCGGGCATGA

Upstream 100 bases:

>100_bases
GTGGGCACCTTTGTCATGGGGATCGTCATCAACCACATCAGCCGCGAATTCTCCTCCCTGGACACCTCGCGCTTGACCTC
CCTGAAAGAGGAGTAGATAC

Downstream 100 bases:

>100_bases
GTCACGGCATGAAGACCTTCCACAACGGCGCCTGCTTCGGGCGCGGCGAGATCCCCCTGCTGGACAACGACGGCTTCTTC
CAGGGAATCCTGGACGCCAC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLAALILLPLAGALLAWLLPGDRFRPALLPLFAFPHLGLTLWLLYRTPLPSPGGWFNLDPLGKIVLLCTSVLFLFCSLYA
LGYLAYRRERSSRILCIGLLVCLSAASLVTTTHHLGLLWVALETTTISMAPLIYFNRNARSIEATWKYLLICSVGVALAL
IGLFFLAYSTIVSGMEPSLLLDDLMRDAPRLSPSWVHAAFVFLLVGFGSKMGLAPLHTWKPDAYGEAPGLVGAMLAGGLV
NLAFLALLRIYQLCLATREIVFFQNALVAMGLISMFLAAVFMARQADFKRMLAYSSVEHVGIMAVALGLGGKAIFGALFH
VFANGLTKGVLFLSSGNIHRSYNSKSTSQVAGALRRLPWSGGLFLAGFIAITGSPPFAPFISEFTIVSSAFMQGRMLVAA
LFLLSLTIIFIGMALTVLPMVMGEVPESSESSPYRDTVWTVGPPLVMLLVVFLLGVWIPEPLLAILRAAASLLEVRA

Sequences:

>Translated_477_residues
MLAALILLPLAGALLAWLLPGDRFRPALLPLFAFPHLGLTLWLLYRTPLPSPGGWFNLDPLGKIVLLCTSVLFLFCSLYA
LGYLAYRRERSSRILCIGLLVCLSAASLVTTTHHLGLLWVALETTTISMAPLIYFNRNARSIEATWKYLLICSVGVALAL
IGLFFLAYSTIVSGMEPSLLLDDLMRDAPRLSPSWVHAAFVFLLVGFGSKMGLAPLHTWKPDAYGEAPGLVGAMLAGGLV
NLAFLALLRIYQLCLATREIVFFQNALVAMGLISMFLAAVFMARQADFKRMLAYSSVEHVGIMAVALGLGGKAIFGALFH
VFANGLTKGVLFLSSGNIHRSYNSKSTSQVAGALRRLPWSGGLFLAGFIAITGSPPFAPFISEFTIVSSAFMQGRMLVAA
LFLLSLTIIFIGMALTVLPMVMGEVPESSESSPYRDTVWTVGPPLVMLLVVFLLGVWIPEPLLAILRAAASLLEVRA
>Mature_477_residues
MLAALILLPLAGALLAWLLPGDRFRPALLPLFAFPHLGLTLWLLYRTPLPSPGGWFNLDPLGKIVLLCTSVLFLFCSLYA
LGYLAYRRERSSRILCIGLLVCLSAASLVTTTHHLGLLWVALETTTISMAPLIYFNRNARSIEATWKYLLICSVGVALAL
IGLFFLAYSTIVSGMEPSLLLDDLMRDAPRLSPSWVHAAFVFLLVGFGSKMGLAPLHTWKPDAYGEAPGLVGAMLAGGLV
NLAFLALLRIYQLCLATREIVFFQNALVAMGLISMFLAAVFMARQADFKRMLAYSSVEHVGIMAVALGLGGKAIFGALFH
VFANGLTKGVLFLSSGNIHRSYNSKSTSQVAGALRRLPWSGGLFLAGFIAITGSPPFAPFISEFTIVSSAFMQGRMLVAA
LFLLSLTIIFIGMALTVLPMVMGEVPESSESSPYRDTVWTVGPPLVMLLVVFLLGVWIPEPLLAILRAAASLLEVRA

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=179, Percent_Identity=29.0502793296089, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788831, Length=433, Percent_Identity=35.796766743649, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788827, Length=383, Percent_Identity=29.7650130548303, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788613, Length=190, Percent_Identity=31.5789473684211, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI2367154, Length=353, Percent_Identity=24.6458923512748, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI145693160, Length=402, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788829, Length=353, Percent_Identity=27.1954674220963, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788614, Length=201, Percent_Identity=30.3482587064677, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51587; Mature: 51587

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAALILLPLAGALLAWLLPGDRFRPALLPLFAFPHLGLTLWLLYRTPLPSPGGWFNLDP
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LGKIVLLCTSVLFLFCSLYALGYLAYRRERSSRILCIGLLVCLSAASLVTTTHHLGLLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
ALETTTISMAPLIYFNRNARSIEATWKYLLICSVGVALALIGLFFLAYSTIVSGMEPSLL
EEHHHHEEEHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LDDLMRDAPRLSPSWVHAAFVFLLVGFGSKMGLAPLHTWKPDAYGEAPGLVGAMLAGGLV
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NLAFLALLRIYQLCLATREIVFFQNALVAMGLISMFLAAVFMARQADFKRMLAYSSVEHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIMAVALGLGGKAIFGALFHVFANGLTKGVLFLSSGNIHRSYNSKSTSQVAGALRRLPWS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCCHHHHHHHHHHHCCCC
GGLFLAGFIAITGSPPFAPFISEFTIVSSAFMQGRMLVAALFLLSLTIIFIGMALTVLPM
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMGEVPESSESSPYRDTVWTVGPPLVMLLVVFLLGVWIPEPLLAILRAAASLLEVRA
HHCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAALILLPLAGALLAWLLPGDRFRPALLPLFAFPHLGLTLWLLYRTPLPSPGGWFNLDP
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LGKIVLLCTSVLFLFCSLYALGYLAYRRERSSRILCIGLLVCLSAASLVTTTHHLGLLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
ALETTTISMAPLIYFNRNARSIEATWKYLLICSVGVALALIGLFFLAYSTIVSGMEPSLL
EEHHHHEEEHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LDDLMRDAPRLSPSWVHAAFVFLLVGFGSKMGLAPLHTWKPDAYGEAPGLVGAMLAGGLV
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NLAFLALLRIYQLCLATREIVFFQNALVAMGLISMFLAAVFMARQADFKRMLAYSSVEHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIMAVALGLGGKAIFGALFHVFANGLTKGVLFLSSGNIHRSYNSKSTSQVAGALRRLPWS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCCHHHHHHHHHHHCCCC
GGLFLAGFIAITGSPPFAPFISEFTIVSSAFMQGRMLVAALFLLSLTIIFIGMALTVLPM
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMGEVPESSESSPYRDTVWTVGPPLVMLLVVFLLGVWIPEPLLAILRAAASLLEVRA
HHCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]