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The map label for this gene is ansA [H]

Identifier: 118478429

GI number: 118478429

Start: 2955636

End: 2956655

Strand: Reverse

Name: ansA [H]

Synonym: BALH_2797

Alternate gene names: 118478429

Gene position: 2956655-2955636 (Counterclockwise)

Preceding gene: 118478431

Following gene: 118478428

Centisome position: 56.24

GC content: 35.78

Gene sequence:

>1020_bases
ATGGAGGGGTTGTGTATGAAGAAAATTTTATTACTTGCAACTGGTGGGACAATTGCATCTGTGGAGGGGAATGAAGGACT
TGCTCCAGGATTGTCCGCTGAGGAATTATTGAATTATTTTTCGAGGTCATCCCGAAATTTAGAAATAGATTGCAAAATCT
TAATGAATATTGATAGTACCAATATGCAACCTGAGCACTGGAAAGAAATAGCTAATGCTGTTTTTAACCACTATGATGAC
TATGACGGGTTTGTAATTACACATGGAACGGATACGTTAGCTTATACATCTTCTGCGTTATCTTATATGCTTCAAGGTTT
AAGAAAACCAGTTGTTTTAACAGGCTCACAAGTACCTATTAGTTTTAAAAAGACAGACGCAAAGAAAAATGTAGCTGATG
CTCTTAGGTTTGCCTGTGAGGATGTTGGCGGTGTGTTTATTGTTTTTGATAGTAGGGTAATTATTGGGACGAGAGCAGTT
AAAATGCGAACAAAAAGTTATGATGCATTTGAAAGTGTGAATTATCCATATGTTGCTGAGGTAAATGAAGATGAAGTGAA
ATATCATTGGAAGCCAACGTCCTCCCATAATGAACTTTCAATAAATACGAATTTATGTACGGATGTTTTTCTTATGAAAT
TGTATCCAGGTACAAAGCCAGAGATATTTGATTGTTTAAAAGGTTTGTATAAAGGGATTATTATTGAAAGTTTTGGTAAC
GGTGGATTACCTTTTGAAGGAAGGAACTTGCTTTCGAAAATTCAAGAGTTGACTGAAATGGGGACTGCTGTAGTGATTAC
TACTCAATGCCTGGAAGAAGGAGAAGACATACTTCTCTATGAAGTAGGACGGAAAGTGGCGCAACATCAAGTAATATTGT
CTGGAGATATGAATACGGAAGCCATCATAGCAAAATTGATGTGGACGCTTGGAAAAACTCATAAATTAGAAGAGATTAAA
AAAATTATTGAAGAGCCTTTAGCCTACGATTTAACAATAAAATCTGATAAGGATTGGTAA

Upstream 100 bases:

>100_bases
AGTACGCGTATTGAATACAAAATCTTGATTAATTCAAATTAAGTTCTATAATGATAAATATAAATACGCATTACGCGAAC
GATATATCGTTCTTTATTTT

Downstream 100 bases:

>100_bases
TGACTTCATATGATTTATTTAGGTTTTACATTTAATATAGGGGAGAAATTAAAATGGCAATGACAAACAAGAACGTGCGA
GTGGAGAAGGATTTTTTGGG

Product: asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MEGLCMKKILLLATGGTIASVEGNEGLAPGLSAEELLNYFSRSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDD
YDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAV
KMRTKSYDAFESVNYPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKGLYKGIIIESFGN
GGLPFEGRNLLSKIQELTEMGTAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWTLGKTHKLEEIK
KIIEEPLAYDLTIKSDKDW

Sequences:

>Translated_339_residues
MEGLCMKKILLLATGGTIASVEGNEGLAPGLSAEELLNYFSRSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDD
YDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAV
KMRTKSYDAFESVNYPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKGLYKGIIIESFGN
GGLPFEGRNLLSKIQELTEMGTAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWTLGKTHKLEEIK
KIIEEPLAYDLTIKSDKDW
>Mature_339_residues
MEGLCMKKILLLATGGTIASVEGNEGLAPGLSAEELLNYFSRSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDD
YDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAV
KMRTKSYDAFESVNYPYVAEVNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKGLYKGIIIESFGN
GGLPFEGRNLLSKIQELTEMGTAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWTLGKTHKLEEIK
KIIEEPLAYDLTIKSDKDW

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=316, Percent_Identity=34.1772151898734, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788065, Length=335, Percent_Identity=33.7313432835821, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789327, Length=303, Percent_Identity=25.0825082508251, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=285, Percent_Identity=32.9824561403509, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558280, Length=285, Percent_Identity=32.9824561403509, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558278, Length=285, Percent_Identity=32.9824561403509, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI193207374, Length=285, Percent_Identity=32.9824561403509, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=25.0755287009063, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=339, Percent_Identity=24.188790560472, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323187, Length=339, Percent_Identity=24.188790560472, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323186, Length=339, Percent_Identity=24.188790560472, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323184, Length=339, Percent_Identity=24.188790560472, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24639609, Length=282, Percent_Identity=34.7517730496454, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24645530, Length=347, Percent_Identity=32.2766570605187, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37861; Mature: 37861

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLCMKKILLLATGGTIASVEGNEGLAPGLSAEELLNYFSRSSRNLEIDCKILMNIDST
CCCHHHHHHHEEECCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCC
NMQPEHWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPI
CCCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCE
SFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNYPYVAE
EEECCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEEEECCCCHHHHCCCCCEEEE
VNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKGLYKGIIIESFGN
CCCCCEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCC
GGLPFEGRNLLSKIQELTEMGTAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTE
CCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHH
AIIAKLMWTLGKTHKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC
>Mature Secondary Structure
MEGLCMKKILLLATGGTIASVEGNEGLAPGLSAEELLNYFSRSSRNLEIDCKILMNIDST
CCCHHHHHHHEEECCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCC
NMQPEHWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPI
CCCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCE
SFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNYPYVAE
EEECCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEEEECCCCHHHHCCCCCEEEE
VNEDEVKYHWKPTSSHNELSINTNLCTDVFLMKLYPGTKPEIFDCLKGLYKGIIIESFGN
CCCCCEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCC
GGLPFEGRNLLSKIQELTEMGTAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTE
CCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHH
AIIAKLMWTLGKTHKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]