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The map label for this gene is aspA [H]

Identifier: 118478428

GI number: 118478428

Start: 2954152

End: 2955582

Strand: Reverse

Name: aspA [H]

Synonym: BALH_2796

Alternate gene names: 118478428

Gene position: 2955582-2954152 (Counterclockwise)

Preceding gene: 118478429

Following gene: 118478425

Centisome position: 56.22

GC content: 39.83

Gene sequence:

>1431_bases
ATGGCAATGACAAACAAGAACGTGCGAGTGGAGAAGGATTTTTTGGGAGGAAAGGAATTACCTATTGAGGCATATTACGG
AATTCAGACTTTACGCGCTGTTGAAAATTTCCCGATTACAGGGTACAAAATTCATGAAAGTTTAATTAGAGCGTTCGCAG
TTGTAAAAAAAGCTGCAGCGCTTGCGAATACAGATGTAGGAAGATTGGAATTGAACAAGGGCGGCGCGATCGCAGAAGCT
GCTCAAGAAATTCTTGATGGAAAATGGCATGATCATTTCATCGTAGATCCAATCCAAGGCGGAGCAGGTACTTCAATGAA
CATGAATGCAAATGAAGTCATGGCCAATCGTGCTCTTGAATTATTAGGGATGGAAAAGGGAGACTATCATTATATTAGTC
CAAATAGTCATGTAAACATGGCGCAATCAACAAATGATGCATTCCCAACGGCAATTCATATCGCAACGTTAAATGCATTA
GAAGGTTTATTACAAACGATGGGTTATATGCATGATGTATTTGAATTAAAAGCAGAGCAGTTCGACCATGTGATTAAAAT
GGGACGTACACATTTACAAGACGCTGTGCCAATTCGTCTTGGACAAGAATTTAAAGCGTACTCTCGCGTGCTTGAACGTG
ATATGAAACGAATTCAGCAATCACGTCAACACTTATATGAAGTGAATATGGGGGCAACTGCAGTTGGTACAGGCTTAAAT
GCAGATCCAGAATATATTGAAGCAGTTGTAAAACATTTAGCTGCAATTAGTGAGCTACCGCTTGTTGGTGCAGAAGACTT
AGTAGATGCAACGCAAAATACGGATGCATATACAGAAGTATCAGCAGCACTTAAAGTATGTATGATGAATATGTCTAAAA
TTGCGAATGACCTTCGCTTAATGGCATCAGGTCCACGTGTTGGCTTAGCGGAAATTATGTTACCTGCTCGTCAACCAGGT
TCATCTATTATGCCAGGGAAAGTAAACCCTGTTATGCCAGAAGTAATTAATCAAATTGCGTTCCAAGTCATTGGTAACGA
CCATACAATTTGCCTTGCTTCAGAAGCAGGTCAATTAGAATTAAACGTTATGGAACCAGTACTTGTTTTCAACTTACTAC
AATCAATTAGCATTATGAATAACGGTTTCCGTGCCTTTACAGATAATTGCTTAAAAGGAATTGAAGCGAATGAAGCTCGC
TTAAAAGAGTATGTTGAGAAGAGTGTAGGAATTATTACAGCCGTGAACCCTCATATCGGTTATGAAGCAGCAGCTCGCGT
TGCGAAAGAAGCAATCGCGACAGGGCAATCCGTTCGAGAACTTTGTGTGAAAAATGGTGTACTGTCACAAGAAGACTTAG
AATTAATTCTAGATCCATTCGAAATGACACACCCAGGGATTGCAGGAGCAACTCTTTTAAAGAAAAATTAA

Upstream 100 bases:

>100_bases
AGCCTTTAGCCTACGATTTAACAATAAAATCTGATAAGGATTGGTAATGACTTCATATGATTTATTTAGGTTTTACATTT
AATATAGGGGAGAAATTAAA

Downstream 100 bases:

>100_bases
AAGAAGAGGGGAACAAGCCCCTCCTCTTTGTTTACAATATAAGGATGTTATAAGTAAGTAAACAGTGGCGTCTAATGGTT
CATAGAAACAAAATTAAGAG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAMTNKNVRVEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAALANTDVGRLELNKGGAIAEA
AQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVMANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNAL
EGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPG
SSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEAR
LKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN

Sequences:

>Translated_476_residues
MAMTNKNVRVEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAALANTDVGRLELNKGGAIAEA
AQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVMANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNAL
EGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPG
SSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEAR
LKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
>Mature_475_residues
AMTNKNVRVEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAALANTDVGRLELNKGGAIAEAA
QEILDGKWHDHFIVDPIQGGAGTSMNMNANEVMANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALE
GLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLNA
DPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPGS
SIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEARL
KEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=465, Percent_Identity=41.9354838709677, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI68303547, Length=245, Percent_Identity=29.7959183673469, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI31541964, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI68303542, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=49.7854077253219, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=42.3326133909287, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=43.1670281995662, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=41.8960244648318, Blast_Score=270, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=41.5948275862069, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640179, Length=469, Percent_Identity=41.5778251599147, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640177, Length=471, Percent_Identity=41.1889596602972, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24662684, Length=471, Percent_Identity=41.6135881104034, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=40.6926406926407, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=37.9310344827586, Blast_Score=338, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51947; Mature: 51815

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTNKNVRVEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAA
CCCCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVMANRALE
HHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
LLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELKAEQ
HHCCCCCCEEEECCCCCEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLE
HCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEARLKEYVEKSVGIITAVNPHIG
EEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
AMTNKNVRVEKDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHESLIRAFAVVKKAAA
CCCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVMANRALE
HHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
LLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELKAEQ
HHCCCCCCEEEECCCCCEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLE
HCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEARLKEYVEKSVGIITAVNPHIG
EEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]