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The map label for this gene is vat [H]

Identifier: 118478015

GI number: 118478015

Start: 2537816

End: 2538472

Strand: Direct

Name: vat [H]

Synonym: BALH_2366

Alternate gene names: 118478015

Gene position: 2537816-2538472 (Clockwise)

Preceding gene: 118478014

Following gene: 118478017

Centisome position: 48.27

GC content: 33.18

Gene sequence:

>657_bases
GTGGTAAAGGAAGTGCTATACAAAATGAATCCTAATCCAAATGTTAAATACCCTATTGAAGGAAATCAAAACGTCCATTT
TATAAAAAATACAATAACAAAAGCTAATATACTCGTTGGAGACTATTCATATTATGATGCGAAAGATGGAGAAACATTTG
AGGATCGAGTATTACATCATTATGAATTTCTTGGAGATCGCCTTTTTATTGGAAAGTTTTGTTGTATTGCAAATGGAGTT
ACCTTTATTATGAACGGAGCAAATCATCGGATGGATGGATTTTCAGCATATCCATTTAATATATTTGGAAATGGGTGGGA
GAAATATACACCTAATTTGTCTGATTTACCTTACAAAGGTGATACAGTTATAGGAAATGACGTTTGGATCGGTATGGATG
TAACGATTATGCCTGGAATAAAAATAGGGGATGGTGCAATAATAGCAGCTAAATCTGTTGTGACGAGAGACGTCGCACCG
TATACAATTGTTGGCGGAAACCCTGCAAATAAAATAAGAGAGAGATTTTCAAATGCAATAATAGAAGAATTATTGCAAAT
TCAATGGTGGCATTTTCATATTGAAAAAATAACTGAAAATATAGATGCGATTGTACAAGGGAATATAGAGCCATTAAGAA
AGCTAAAAAGGGAATAA

Upstream 100 bases:

>100_bases
ATGATGTATGAAGGAACGGCTTTCCTATTTATAATTGGTTTATTGTTTATTTTACCTATATACAATTTAAAGCCAACGAT
AGCAATAGAAAGTGAAATAA

Downstream 100 bases:

>100_bases
AAAATGCATAACAAAGAGTATCATACCTCCGAATTTTGTTAATGATAATAGAACAAGGAGGTGTGATACTATGGCACAAG
ACGTTTTATGTGAAGTAAAC

Product: virginiamycin A acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MVKEVLYKMNPNPNVKYPIEGNQNVHFIKNTITKANILVGDYSYYDAKDGETFEDRVLHHYEFLGDRLFIGKFCCIANGV
TFIMNGANHRMDGFSAYPFNIFGNGWEKYTPNLSDLPYKGDTVIGNDVWIGMDVTIMPGIKIGDGAIIAAKSVVTRDVAP
YTIVGGNPANKIRERFSNAIIEELLQIQWWHFHIEKITENIDAIVQGNIEPLRKLKRE

Sequences:

>Translated_218_residues
MVKEVLYKMNPNPNVKYPIEGNQNVHFIKNTITKANILVGDYSYYDAKDGETFEDRVLHHYEFLGDRLFIGKFCCIANGV
TFIMNGANHRMDGFSAYPFNIFGNGWEKYTPNLSDLPYKGDTVIGNDVWIGMDVTIMPGIKIGDGAIIAAKSVVTRDVAP
YTIVGGNPANKIRERFSNAIIEELLQIQWWHFHIEKITENIDAIVQGNIEPLRKLKRE
>Mature_218_residues
MVKEVLYKMNPNPNVKYPIEGNQNVHFIKNTITKANILVGDYSYYDAKDGETFEDRVLHHYEFLGDRLFIGKFCCIANGV
TFIMNGANHRMDGFSAYPFNIFGNGWEKYTPNLSDLPYKGDTVIGNDVWIGMDVTIMPGIKIGDGAIIAAKSVVTRDVAP
YTIVGGNPANKIRERFSNAIIEELLQIQWWHFHIEKITENIDAIVQGNIEPLRKLKRE

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 24773; Mature: 24773

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKEVLYKMNPNPNVKYPIEGNQNVHFIKNTITKANILVGDYSYYDAKDGETFEDRVLHH
CCHHHEECCCCCCCEEEECCCCCCEEEEECCCCEEEEEEECCCEECCCCCCCHHHHHHHH
YEFLGDRLFIGKFCCIANGVTFIMNGANHRMDGFSAYPFNIFGNGWEKYTPNLSDLPYKG
HHHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEECCEEEECCCHHHCCCCCCCCCCCC
DTVIGNDVWIGMDVTIMPGIKIGDGAIIAAKSVVTRDVAPYTIVGGNPANKIRERFSNAI
CEEECCCEEEEEEEEEECCEEECCCEEEEEHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
IEELLQIQWWHFHIEKITENIDAIVQGNIEPLRKLKRE
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCC
>Mature Secondary Structure
MVKEVLYKMNPNPNVKYPIEGNQNVHFIKNTITKANILVGDYSYYDAKDGETFEDRVLHH
CCHHHEECCCCCCCEEEECCCCCCEEEEECCCCEEEEEEECCCEECCCCCCCHHHHHHHH
YEFLGDRLFIGKFCCIANGVTFIMNGANHRMDGFSAYPFNIFGNGWEKYTPNLSDLPYKG
HHHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEECCEEEECCCHHHCCCCCCCCCCCC
DTVIGNDVWIGMDVTIMPGIKIGDGAIIAAKSVVTRDVAPYTIVGGNPANKIRERFSNAI
CEEECCCEEEEEEEEEECCEEECCCEEEEEHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
IEELLQIQWWHFHIEKITENIDAIVQGNIEPLRKLKRE
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]