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The map label for this gene is ddl [H]

Identifier: 118478002

GI number: 118478002

Start: 2520986

End: 2521921

Strand: Direct

Name: ddl [H]

Synonym: BALH_2350

Alternate gene names: 118478002

Gene position: 2520986-2521921 (Clockwise)

Preceding gene: 118478001

Following gene: 118478003

Centisome position: 47.95

GC content: 34.19

Gene sequence:

>936_bases
ATGAAATTTGGAGTTGAGGAAATGAGAATGGGCGTTATTATGGGAGGAGTATCCTCTGAAAAACAAGTATCAATTATGAC
TGGAAATGAAATGATTGCACATTTAGATAAAAATAAATATGAAATAGTACCAATTACGTTAAATGAAAAAATGGATTTAA
TCGAAAAAGCAAAAGACATTGATTTTGCACTGCTCGCATTACATGGAAAATACGGAGAAGATGGGACAGTTCAAGGAACG
CTTGAAAGTTTAGGTATTCCTTATAGCGGGAGCAATATGTTATCTAGCGGTATATGTATGGACAAAAACATGTCAAAAAA
GATTTTACGTTATGAAGGAATAGAAACACCAGATTGGATTGAACTTACAAAAATGGAGGATTTGAATTTTGATGAGTTAG
ATAAGTTAGGATTTCCATTAGTGGTAAAACCAAACTCTGGTGGTTCAAGTGTTGGGGTAAAGATTGTCTATGATAAAGAC
GAATTAATTTCAACGCTGGAAACTGTGTTCGAATGGGATTCTGAAGTAGTAATAGAAAAGTATATTAAAGGTGATGAGAT
TACATGTTCCATTTTTGATGGAAAACAGTTACCTATCATTTCAATTCGACATGCGGCTGAGTTCTTTGATTACAATGCGA
AATATGATGATGCAGGTACTATTGAAGAAGTTATCGAACTTCCAGCTGAACTTAAGGAACGTGTAAACAAAGCGTCACTG
GCGTGCTATAAAGCATTAAAATGTAGTGTTTATGCAAGGGTTGATATGATGGTGAAGGACGGTATTCCATATGTAATGGA
GGTTAATACATTACCAGGGATGACACAAGCGAGTTTACTACCAAAAAGTGCAGAAGCAGAGGGAATTGATTATAGTAAAC
TATTAGATATGATTATTGAAACTTCATTAAGAGTAAGAAAAGAAGAAGGGTTTTAA

Upstream 100 bases:

>100_bases
TCTAATGGAGAAGGAATAAACACATTTCGATTAGGATTTTCACGTCTGAAAGAAGAAGAAATAGTTCGGGGAATCAAAAT
AATCGGTGATACAATAAAAA

Downstream 100 bases:

>100_bases
GCAATGGTATCATGAATACTTTTGACAGTAACAAGAAGTATGTTATGATGAATACGATACGAAAATAAAATATTTATCCA
CTAGGGGGGCCTATTATAGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKFGVEEMRMGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGT
LESLGIPYSGSNMLSSGICMDKNMSKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKD
ELISTLETVFEWDSEVVIEKYIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASL
ACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSAEAEGIDYSKLLDMIIETSLRVRKEEGF

Sequences:

>Translated_311_residues
MKFGVEEMRMGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGT
LESLGIPYSGSNMLSSGICMDKNMSKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKD
ELISTLETVFEWDSEVVIEKYIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASL
ACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSAEAEGIDYSKLLDMIIETSLRVRKEEGF
>Mature_311_residues
MKFGVEEMRMGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGT
LESLGIPYSGSNMLSSGICMDKNMSKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKD
ELISTLETVFEWDSEVVIEKYIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASL
ACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSAEAEGIDYSKLLDMIIETSLRVRKEEGF

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=301, Percent_Identity=35.8803986710963, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1786579, Length=352, Percent_Identity=29.5454545454545, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 34708; Mature: 34708

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFGVEEMRMGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDI
CCCCHHHHHHEEEECCCCCCCEEEEEECCCEEEEECCCCEEEEEEEECCHHHHHHHHCCC
DFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNMSKKILRYEGIETPDWI
CEEEEEEECCCCCCCCEEEEHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCCCE
ELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISTLETVFEWDSEVVIEK
EEEECCCCCHHHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCHHHHHH
YIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASL
HCCCCCEEEEEECCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHH
ACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSAEAEGIDYSKLLDMIIE
HHHHHHHHEEEHEEEEHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
TSLRVRKEEGF
HHHHHHHCCCC
>Mature Secondary Structure
MKFGVEEMRMGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMDLIEKAKDI
CCCCHHHHHHEEEECCCCCCCEEEEEECCCEEEEECCCCEEEEEEEECCHHHHHHHHCCC
DFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNMSKKILRYEGIETPDWI
CEEEEEEECCCCCCCCEEEEHHHCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCCCE
ELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISTLETVFEWDSEVVIEK
EEEECCCCCHHHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCHHHHHH
YIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDAGTIEEVIELPAELKERVNKASL
HCCCCCEEEEEECCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHH
ACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSAEAEGIDYSKLLDMIIE
HHHHHHHHEEEHEEEEHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
TSLRVRKEEGF
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA