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The map label for this gene is tenA [H]

Identifier: 118478003

GI number: 118478003

Start: 2522175

End: 2522912

Strand: Direct

Name: tenA [H]

Synonym: BALH_2351

Alternate gene names: 118478003

Gene position: 2522175-2522912 (Clockwise)

Preceding gene: 118478002

Following gene: 118478004

Centisome position: 47.98

GC content: 35.37

Gene sequence:

>738_bases
TTGCTGTGGATAAATACTTATATGAAATGGATTATTGGAGGGAATCAAATGACTTTTTCACAATCATTACGTAAAGAAGT
AGATTCAATTTGGGAAGCTAGTTTTCATCATCCTTTTGTAAAAAAACTCGGTGAAGGAACGTTAGATTTAGCTAATTTTC
GCTATTACGTGCTTCAAGATTCCTATTATTTGAGTCACTTTGCTAGAGTACAAACATTAGGAGCTGCAAAAGCGCTTGAA
TTAGAAACGACTGCTCGCATGGCACATCATGCTCAAAATACATATGAGGCGGAATTATCTTTACATGAGAACTTTGCGAA
GAAACTAGGGATTACACAAGAAGAAAAAGATAATTTTATCCCTGCTCCAACTGCATATGCATATACTTCACATATGTACC
GTGCTGCGTATGAAGGACATTTAGGCGATATTATTGCAGCAATTTTACCTTGCTATTGGCTGTATTATGAAATTGGTGAG
CGTTTAAAAGAGTGTCAGCCAGAAGAGCCGATTTATAACGAATGGATTTCTGCTTATGGATCTGATTGGTTCCGAACATT
AGTTGAAGAGCAAATTACACGATTAGATGCAATAGCTGAAAAAGTGACAGAGTCTGATCGTAAGCGTATGAAACAGCATT
TTATTATAAGTAGTCAATATGAATATTCATTTTGGGAAATGGCTTATACTTTAGAAAAGTGGCCGGTAGATACAGAAGTA
AAAGATGTAATAAGATAA

Upstream 100 bases:

>100_bases
CGTAGGGAAGTGGGAAAGACATTGCTATTTCATGTATAAATACTGTCAATTTCACTTTCTTTACGCCTGTAAAGAAAGTT
TTTTTATTATAAAGCGTTAA

Downstream 100 bases:

>100_bases
AGAAAAGAGGTATTGCTATACGTATGTATGGACAATACCTCTTTTTAGGTTTTTATAAAATCATTGTAAAAATCACAAAT
AAAACACTCCTTTATATTGT

Product: transcriptional activator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MLWINTYMKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALE
LETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGE
RLKECQPEEPIYNEWISAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEV
KDVIR

Sequences:

>Translated_245_residues
MLWINTYMKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALE
LETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGE
RLKECQPEEPIYNEWISAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEV
KDVIR
>Mature_245_residues
MLWINTYMKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALE
LETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGE
RLKECQPEEPIYNEWISAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEV
KDVIR

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=202, Percent_Identity=26.7326732673267, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6325378, Length=205, Percent_Identity=25.8536585365854, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324997, Length=201, Percent_Identity=21.8905472636816, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 28876; Mature: 28876

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWINTYMKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQD
CEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEHHHCEEEEEEC
SYYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCC
PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAYG
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
SDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHH
KDVIR
HHHCC
>Mature Secondary Structure
MLWINTYMKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQD
CEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEHHHCEEEEEEC
SYYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCC
PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAYG
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
SDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDTEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHH
KDVIR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]