Click here to switch to the map view.

The map label for this gene is def2 [H]

Identifier: 117919351

GI number: 117919351

Start: 1051477

End: 1052007

Strand: Reverse

Name: def2 [H]

Synonym: Shewana3_0900

Alternate gene names: 117919351

Gene position: 1052007-1051477 (Counterclockwise)

Preceding gene: 117919352

Following gene: 117919350

Centisome position: 21.16

GC content: 48.02

Gene sequence:

>531_bases
ATGTTTAAAGATGAGATGTTACCAGCCCCCAATCAGCCACTACCAATAGCCTTGGTGGGTGAAGCCATTTTAAAACAGCA
GGCGGATGAAGTACGCGACTTTGATGAAAAGCTCAGTCATTTAGCATCGCAAATGGTGGCAAGCATGGTTGAGGCAAAAG
GTGTCGGAATTGCCGCTCCTCAAGTCCATAGCCCACTCGCCCTGTTTATTATGGCATCCCGGCCAAACGAGCGTTATCCC
GATGCGCCGCTAATGGACCCTGTCGTGGTGGTTAATCCACAGATCTTGTCTGCCTCTGCGGAATTAGTTAGCGGTGAAGA
AGGCTGTTTATCGGTTCCCGGCCAAAGGTTTAGCATTTGGCGCCACCAGACGATTGTCGTGCGCTATCAAGATCTTGCAG
GACACTGGCAACAGACTGAGTTAACAGGTTTTATCGCTCGTATTTTCCAACATGAGTTTGATCATCTACAAGGGATCACT
TTGCTTGAACGCTCTCAAATGCCAGAACAAAAACTAATGGCAAGAAGGTAA

Upstream 100 bases:

>100_bases
CGACTCAAGCAGAAGAAACACCACCGCCACATTACTAATTCAATAACGGATAATCCAATCCATTAACGACAATATGACCA
CTAAATATCCAGGGAATGCG

Downstream 100 bases:

>100_bases
ACCACAGGCATGAAAACCACCTTCATATACTCTTTTATTAAACCCACACTTAGCACGCTAATGCTCGCCGCCATCTTAGG
CTCGAGCGGCTGTGCCTATA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MFKDEMLPAPNQPLPIALVGEAILKQQADEVRDFDEKLSHLASQMVASMVEAKGVGIAAPQVHSPLALFIMASRPNERYP
DAPLMDPVVVVNPQILSASAELVSGEEGCLSVPGQRFSIWRHQTIVVRYQDLAGHWQQTELTGFIARIFQHEFDHLQGIT
LLERSQMPEQKLMARR

Sequences:

>Translated_176_residues
MFKDEMLPAPNQPLPIALVGEAILKQQADEVRDFDEKLSHLASQMVASMVEAKGVGIAAPQVHSPLALFIMASRPNERYP
DAPLMDPVVVVNPQILSASAELVSGEEGCLSVPGQRFSIWRHQTIVVRYQDLAGHWQQTELTGFIARIFQHEFDHLQGIT
LLERSQMPEQKLMARR
>Mature_176_residues
MFKDEMLPAPNQPLPIALVGEAILKQQADEVRDFDEKLSHLASQMVASMVEAKGVGIAAPQVHSPLALFIMASRPNERYP
DAPLMDPVVVVNPQILSASAELVSGEEGCLSVPGQRFSIWRHQTIVVRYQDLAGHWQQTELTGFIARIFQHEFDHLQGIT
LLERSQMPEQKLMARR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=137, Percent_Identity=31.3868613138686, Blast_Score=71, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.0126582278481, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19692; Mature: 19692

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKDEMLPAPNQPLPIALVGEAILKQQADEVRDFDEKLSHLASQMVASMVEAKGVGIAAP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QVHSPLALFIMASRPNERYPDAPLMDPVVVVNPQILSASAELVSGEEGCLSVPGQRFSIW
CCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEECCCCCEEHE
RHQTIVVRYQDLAGHWQQTELTGFIARIFQHEFDHLQGITLLERSQMPEQKLMARR
ECEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MFKDEMLPAPNQPLPIALVGEAILKQQADEVRDFDEKLSHLASQMVASMVEAKGVGIAAP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QVHSPLALFIMASRPNERYPDAPLMDPVVVVNPQILSASAELVSGEEGCLSVPGQRFSIW
CCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEECCCCCEEHE
RHQTIVVRYQDLAGHWQQTELTGFIARIFQHEFDHLQGITLLERSQMPEQKLMARR
ECEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]