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The map label for this gene is pitA [H]

Identifier: 117919344

GI number: 117919344

Start: 1038528

End: 1039997

Strand: Direct

Name: pitA [H]

Synonym: Shewana3_0892

Alternate gene names: 117919344

Gene position: 1038528-1039997 (Clockwise)

Preceding gene: 117919343

Following gene: 117919346

Centisome position: 20.89

GC content: 50.95

Gene sequence:

>1470_bases
ATGTTTGAGATATTGACTTCTATCGGCCTGGGTTGGTCGGTAGCTTTAGTGCTGGCGGTGTTATTTGTGCTGGCCTACGA
ATTTATCAATGGTTTCCACGATACCGCCAATGCTGTTGCCACAGTCATCTACACCAAGGCAATGCCTGCCAACCTCGCGG
TGATTTCTTCAGCCCTGTTCAACTTTGCCGGAGTGCTGCTCGGTGGACTTGGCGTCGCCTACGCTATAGTGCATCTCTTG
CCGGTCGATTCTTTACTGGGAATGGATTCAACCCAAGGGCTGTTGATGGTGTTCTCACTACTCTTTTCGGCCATTATTTG
GAACTTAGGGACTTGGTATTTTGGTATTCCGGCCTCCAGTTCCCACACTCTGATTGGCTCGATTATGGGTGTTGGCGGCG
CATTCGCCTGGATACACAATGAACCTGTACTGCACGGCATTAATCTTACCAAAGCCACGCAAATTATGCTGTCGTTGATC
ATTTCGCCCACGCTGGGATTTGTGTTGGCGGGGGTACTGTTATTGCTAATGAAGTTTGTGTGGCAAAAGCACAAGATCCA
CCGCACCCCCGATGAACAATTTCAGATTAATGGCAAACGCCATCCGCCGTTCTGGGCGCGCGTCAGTTTGATTGCCTCGG
CCATGGGGGTGAGCTTTGCCCACGGTTCTAACGATGGCCAAAAGGGCATTGGCTTAGTGATGCTGGTACTGATCTGTATG
GCGCCAGCGTATTTTGCCCTCGACATGAGTAGCCAATCCTATGATTTAGAAAGAACACAGGATGCTAACCGCCGCATTAT
GGCTATCTACAGTCGCAATCAAGCTGTGATTAGCGATCTGGTGGATGTTAAAGCCCAAGTGAATGTGACTGACACTTTAC
TGAGCCACTGCTCGGCACAAACCGTATTACCGGCTATGTCGCTGCTCGATCGCCGTTTAGATAAGGCCAGCAGTTTTCAA
GAGATGAGTGTCGAAGAACGTCGTGAAGTGCGCCGCTTGCTGCTCTGTATCGATGATACTGCGCGTAAAGTGGCAAAATT
AGACTTACCCGCCAAGGAGTTAACATCACTCAATAAATGGCGTAAGGACTTAACTAAGTCAACCGAATACGCGCCTTTGT
GGGTGATAGTGGCCATTGCGACGGCCTTGGGCTGTGGCACCTTAGTGGGTTGGCGCCGCATTGTTTATACCGTCGGTGAG
AAGATAGGCTCCTCGGGGATGACCTACAGCCAAGGGATTGCGGCGCAGGTGACGGCGGCGGCATCGATTGGGATTGCTAG
CGTGACGGGGATGCCAGTTTCAACCACCCATATTCTGTCTTCGGCGGTGGCGGGCACTATGGTGGCCAATCGCTCAGGCC
TACAAAGCCAAACCATTAAGCAGATTTTATTAGCTTGGGTACTGACACTGCCGATTACCATGCTCCTATCTGCATTGGTG
TTTATCTTGGCTAACGCGCTCTGGGGTTAA

Upstream 100 bases:

>100_bases
GAGGACTGATTCACTTTTTGCAAATCTAAATAATTTTTCACTGTCAAAGAGGCCAACCTTAGGCGTAACCTGTGGCGTCC
TCGTTATAGGTTAGCGATTC

Downstream 100 bases:

>100_bases
GCTAAAGTCCTTTTACCACGTCAGTTGCATCTGCGCGGCAGACTCTGCCAACGCTTTATGCAACTGAATAGTGGGCAAGC
TGACCTCATCGGCCAGTTGT

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MFEILTSIGLGWSVALVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVISSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWIHNEPVLHGINLTKATQIMLSLI
ISPTLGFVLAGVLLLLMKFVWQKHKIHRTPDEQFQINGKRHPPFWARVSLIASAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALDMSSQSYDLERTQDANRRIMAIYSRNQAVISDLVDVKAQVNVTDTLLSHCSAQTVLPAMSLLDRRLDKASSFQ
EMSVEERREVRRLLLCIDDTARKVAKLDLPAKELTSLNKWRKDLTKSTEYAPLWVIVAIATALGCGTLVGWRRIVYTVGE
KIGSSGMTYSQGIAAQVTAAASIGIASVTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQILLAWVLTLPITMLLSALV
FILANALWG

Sequences:

>Translated_489_residues
MFEILTSIGLGWSVALVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVISSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWIHNEPVLHGINLTKATQIMLSLI
ISPTLGFVLAGVLLLLMKFVWQKHKIHRTPDEQFQINGKRHPPFWARVSLIASAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALDMSSQSYDLERTQDANRRIMAIYSRNQAVISDLVDVKAQVNVTDTLLSHCSAQTVLPAMSLLDRRLDKASSFQ
EMSVEERREVRRLLLCIDDTARKVAKLDLPAKELTSLNKWRKDLTKSTEYAPLWVIVAIATALGCGTLVGWRRIVYTVGE
KIGSSGMTYSQGIAAQVTAAASIGIASVTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQILLAWVLTLPITMLLSALV
FILANALWG
>Mature_489_residues
MFEILTSIGLGWSVALVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVISSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWIHNEPVLHGINLTKATQIMLSLI
ISPTLGFVLAGVLLLLMKFVWQKHKIHRTPDEQFQINGKRHPPFWARVSLIASAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALDMSSQSYDLERTQDANRRIMAIYSRNQAVISDLVDVKAQVNVTDTLLSHCSAQTVLPAMSLLDRRLDKASSFQ
EMSVEERREVRRLLLCIDDTARKVAKLDLPAKELTSLNKWRKDLTKSTEYAPLWVIVAIATALGCGTLVGWRRIVYTVGE
KIGSSGMTYSQGIAAQVTAAASIGIASVTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQILLAWVLTLPITMLLSALV
FILANALWG

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=470, Percent_Identity=51.7021276595745, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1789360, Length=470, Percent_Identity=51.9148936170213, Blast_Score=475, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 52808; Mature: 52808

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEILTSIGLGWSVALVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVISSALF
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NFAGVLLGGLGVAYAIVHLLPVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGSIMGVGGAFAWIHNEPVLHGINLTKATQIMLSLIISPTLGFVLAGVLLLLMKFV
CCHHHHHHHHCCCCEEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKHKIHRTPDEQFQINGKRHPPFWARVSLIASAMGVSFAHGSNDGQKGIGLVMLVLICM
HHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
APAYFALDMSSQSYDLERTQDANRRIMAIYSRNQAVISDLVDVKAQVNVTDTLLSHCSAQ
CCHHHEEECCCCCCCCHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHH
TVLPAMSLLDRRLDKASSFQEMSVEERREVRRLLLCIDDTARKVAKLDLPAKELTSLNKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RKDLTKSTEYAPLWVIVAIATALGCGTLVGWRRIVYTVGEKIGSSGMTYSQGIAAQVTAA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHH
ASIGIASVTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQILLAWVLTLPITMLLSALV
HHCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FILANALWG
HHHHHHHCC
>Mature Secondary Structure
MFEILTSIGLGWSVALVLAVLFVLAYEFINGFHDTANAVATVIYTKAMPANLAVISSALF
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NFAGVLLGGLGVAYAIVHLLPVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGSIMGVGGAFAWIHNEPVLHGINLTKATQIMLSLIISPTLGFVLAGVLLLLMKFV
CCHHHHHHHHCCCCEEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKHKIHRTPDEQFQINGKRHPPFWARVSLIASAMGVSFAHGSNDGQKGIGLVMLVLICM
HHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
APAYFALDMSSQSYDLERTQDANRRIMAIYSRNQAVISDLVDVKAQVNVTDTLLSHCSAQ
CCHHHEEECCCCCCCCHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHH
TVLPAMSLLDRRLDKASSFQEMSVEERREVRRLLLCIDDTARKVAKLDLPAKELTSLNKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RKDLTKSTEYAPLWVIVAIATALGCGTLVGWRRIVYTVGEKIGSSGMTYSQGIAAQVTAA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHH
ASIGIASVTGMPVSTTHILSSAVAGTMVANRSGLQSQTIKQILLAWVLTLPITMLLSALV
HHCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FILANALWG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]