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The map label for this gene is cysN [H]

Identifier: 117919316

GI number: 117919316

Start: 1006357

End: 1007799

Strand: Direct

Name: cysN [H]

Synonym: Shewana3_0864

Alternate gene names: 117919316

Gene position: 1006357-1007799 (Clockwise)

Preceding gene: 117919315

Following gene: 117919317

Centisome position: 20.24

GC content: 50.17

Gene sequence:

>1443_bases
ATGGATAAAGCGGTCAATAAAACCAATCGTATGGCGGCAGAAATTAGCCAGCACGGGGTAAAAGAATATCTAGCCCAGCA
ACAACATAAAGGTTTGTTGCGCTTTTTAACCTGCGGCAGCGTAGACGATGGCAAGAGCACCCTGATTGGCCGTTTACTGC
ATGACAGCGCGCAGATTTATGAAGACCAACTCGCTAGCTTAAAAAATGATAGCGCTAAGATGGGCACAACGGGCGAAGCC
ATCGACTTGGCTTTGTTGGTCGATGGTCTGCAAGCCGAGCGTGAGCAGGGCATTACTATCGATGTGGCTTATCGTTATTT
TTCAAGCGACAAGCGTAAGTTTATTATCGCCGACACCCCAGGCCATGAGCAGTACACCCGTAATATGGCGACTGGCGCCT
CAACCTGTGATTTAGCAGTGATCTTAGTGGATGCGCGTTACGGCGTGCAGACTCAAACCAAGCGTCATGCCTTTATTGCC
TCTTTGCTGGGTATTCGCCACTTTGTGGTTGCGGTTAATAAGATGGATTTACTCGGGTTCGATGAGCAAGTCTTCAATCG
TATCCGTAATGATTTCAGTGAGTTCGTAAAAGATTTTGGCGACTTAGATATCCACTTTGTGCCTTTATCAGCCCTCAATG
GCGATAACGTGGTTGAGCCGAGTCTGCATACGCCTTGGTATCAGGGCGGCACACTGCTGGAGTTACTCGAAACCATTGAT
ACCCAACGTGAACTCAGCGCCTTGCCTGTACGCTTCCCGGTGCAATATGTGTCGCGTCCCAATCTCGATTTTCGTGGTTT
TGCCGGTACTTTAGCCTCTGGCGTCATCAAGGTGGGTGACGAGGTGGTTGCGCTGCCTTCGGGTAAGCGTAGCAAGGTTG
AACGCATTGTGACCTTCGATGGCGATCTCGCCGAAGCAACGGCTGGGCAAGCCGTCACTATCACCTTAGAAGATGAAATC
GATATTTCCCGCGGTGACTTACTTGCACTGCCAGAGAGTGCGCCACAGGTTGCCAATCAAATCGTGGCTGACCTAGTGTG
GATGGATGAAAAACCATTACAACTCGGCCAGTTATACGACATTAAAGTCGCGGGTAAGAAGACCCAAGCCTCTGTTACGG
CCATCGAGTATGTGGTTGATGTGAACACACTTGCGCGCAGCAGCGCCGACACTTTAGGGCTCAATGCCATCGCCCGCGTG
ACCTTAGAGCTGACCGAAGATATCGTCTTCGATGCCTATTCTCTGGTGCGTGATACTGGCGGCATGATCCTGATTGACCG
TTTATCCAATGCTACTGTTGCGGCTGTGATGGTGGTATCGGGACAGCATGTGAACAAGCAAGCGTCCTCACAATTTAGCG
CCTTTGAAATCGAGTTTAATGCGCTGGTGCGTAAGCACTTCCCGCATTGGCAAGCGATTGATATTTCAAAGCTTGGAGCC
TAA

Upstream 100 bases:

>100_bases
CAGATTAAACCGATTTCGTGAATGTCCCGATGAAGGCTCTTAGAGCAATCGAGTACCAGTGACTTCACGAACAGTTGAGC
AAGATAAGAGTGAGATAAAG

Downstream 100 bases:

>100_bases
TACGTGAGTGACTTGTGGCTATTAGCTATAGTGCTGTTTGGATTAGTGGCTGGCTTAATCGCCGGCCGCATTAATCCCGC
TGTGTTGTTTTTATTTGCTT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MDKAVNKTNRMAAEISQHGVKEYLAQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKNDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRNDFSEFVKDFGDLDIHFVPLSALNGDNVVEPSLHTPWYQGGTLLELLETID
TQRELSALPVRFPVQYVSRPNLDFRGFAGTLASGVIKVGDEVVALPSGKRSKVERIVTFDGDLAEATAGQAVTITLEDEI
DISRGDLLALPESAPQVANQIVADLVWMDEKPLQLGQLYDIKVAGKKTQASVTAIEYVVDVNTLARSSADTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQASSQFSAFEIEFNALVRKHFPHWQAIDISKLGA

Sequences:

>Translated_480_residues
MDKAVNKTNRMAAEISQHGVKEYLAQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKNDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRNDFSEFVKDFGDLDIHFVPLSALNGDNVVEPSLHTPWYQGGTLLELLETID
TQRELSALPVRFPVQYVSRPNLDFRGFAGTLASGVIKVGDEVVALPSGKRSKVERIVTFDGDLAEATAGQAVTITLEDEI
DISRGDLLALPESAPQVANQIVADLVWMDEKPLQLGQLYDIKVAGKKTQASVTAIEYVVDVNTLARSSADTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQASSQFSAFEIEFNALVRKHFPHWQAIDISKLGA
>Mature_480_residues
MDKAVNKTNRMAAEISQHGVKEYLAQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKNDSAKMGTTGEA
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA
SLLGIRHFVVAVNKMDLLGFDEQVFNRIRNDFSEFVKDFGDLDIHFVPLSALNGDNVVEPSLHTPWYQGGTLLELLETID
TQRELSALPVRFPVQYVSRPNLDFRGFAGTLASGVIKVGDEVVALPSGKRSKVERIVTFDGDLAEATAGQAVTITLEDEI
DISRGDLLALPESAPQVANQIVADLVWMDEKPLQLGQLYDIKVAGKKTQASVTAIEYVVDVNTLARSSADTLGLNAIARV
TLELTEDIVFDAYSLVRDTGGMILIDRLSNATVAAVMVVSGQHVNKQASSQFSAFEIEFNALVRKHFPHWQAIDISKLGA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503475, Length=358, Percent_Identity=30.4469273743017, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI223555963, Length=459, Percent_Identity=28.9760348583878, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI5729864, Length=459, Percent_Identity=28.9760348583878, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI4503471, Length=335, Percent_Identity=31.044776119403, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI194097354, Length=329, Percent_Identity=29.483282674772, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI194018522, Length=329, Percent_Identity=29.483282674772, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI194018520, Length=329, Percent_Identity=29.483282674772, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI46094014, Length=309, Percent_Identity=30.0970873786408, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI34147630, Length=326, Percent_Identity=29.7546012269939, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1789108, Length=462, Percent_Identity=64.2857142857143, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1790412, Length=312, Percent_Identity=28.8461538461538, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789737, Length=312, Percent_Identity=28.8461538461538, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI2367247, Length=302, Percent_Identity=26.8211920529801, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17552884, Length=353, Percent_Identity=31.728045325779, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=353, Percent_Identity=31.728045325779, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=296, Percent_Identity=31.4189189189189, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI115532065, Length=296, Percent_Identity=31.4189189189189, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32566629, Length=435, Percent_Identity=25.2873563218391, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566303, Length=346, Percent_Identity=30.0578034682081, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25141371, Length=287, Percent_Identity=27.1777003484321, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=352, Percent_Identity=25.2840909090909, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325337, Length=331, Percent_Identity=31.1178247734139, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=331, Percent_Identity=31.1178247734139, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=408, Percent_Identity=29.656862745098, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=327, Percent_Identity=29.9694189602446, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324761, Length=327, Percent_Identity=27.82874617737, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=192, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550900, Length=442, Percent_Identity=27.6018099547511, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652838, Length=309, Percent_Identity=31.0679611650485, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17137572, Length=309, Percent_Identity=31.0679611650485, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553807, Length=309, Percent_Identity=30.42071197411, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553816, Length=309, Percent_Identity=30.42071197411, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24651721, Length=309, Percent_Identity=30.42071197411, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17864154, Length=309, Percent_Identity=30.42071197411, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=27.1137026239067, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281363316, Length=311, Percent_Identity=29.903536977492, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17864358, Length=311, Percent_Identity=29.903536977492, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19921738, Length=260, Percent_Identity=28.4615384615385, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52598; Mature: 52598

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKAVNKTNRMAAEISQHGVKEYLAQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKNDSAKMGTTGEAIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIASLLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCC
DEQVFNRIRNDFSEFVKDFGDLDIHFVPLSALNGDNVVEPSLHTPWYQGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHC
TQRELSALPVRFPVQYVSRPNLDFRGFAGTLASGVIKVGDEVVALPSGKRSKVERIVTFD
CHHHHHCCCEECCHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCHHHEEEEEEEC
GDLAEATAGQAVTITLEDEIDISRGDLLALPESAPQVANQIVADLVWMDEKPLQLGQLYD
CCHHHHCCCCEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
IKVAGKKTQASVTAIEYVVDVNTLARSSADTLGLNAIARVTLELTEDIVFDAYSLVRDTG
EEEECCCCHHHHHHHHHEEEHHHHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GMILIDRLSNATVAAVMVVSGQHVNKQASSQFSAFEIEFNALVRKHFPHWQAIDISKLGA
CEEEEEECCCCEEEEEEEECCCCCCHHHHCCCEEEEEEHHHHHHHHCCCCCEECHHHCCC
>Mature Secondary Structure
MDKAVNKTNRMAAEISQHGVKEYLAQQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKNDSAKMGTTGEAIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIASLLGIRHFVVAVNKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCC
DEQVFNRIRNDFSEFVKDFGDLDIHFVPLSALNGDNVVEPSLHTPWYQGGTLLELLETID
CHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHC
TQRELSALPVRFPVQYVSRPNLDFRGFAGTLASGVIKVGDEVVALPSGKRSKVERIVTFD
CHHHHHCCCEECCHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCHHHEEEEEEEC
GDLAEATAGQAVTITLEDEIDISRGDLLALPESAPQVANQIVADLVWMDEKPLQLGQLYD
CCHHHHCCCCEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
IKVAGKKTQASVTAIEYVVDVNTLARSSADTLGLNAIARVTLELTEDIVFDAYSLVRDTG
EEEECCCCHHHHHHHHHEEEHHHHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GMILIDRLSNATVAAVMVVSGQHVNKQASSQFSAFEIEFNALVRKHFPHWQAIDISKLGA
CEEEEEECCCCEEEEEEEECCCCCCHHHHCCCEEEEEEHHHHHHHHCCCCCEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]