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The map label for this gene is cysD [H]

Identifier: 117919315

GI number: 117919315

Start: 1005319

End: 1006227

Strand: Direct

Name: cysD [H]

Synonym: Shewana3_0863

Alternate gene names: 117919315

Gene position: 1005319-1006227 (Clockwise)

Preceding gene: 117919314

Following gene: 117919316

Centisome position: 20.22

GC content: 48.84

Gene sequence:

>909_bases
ATGGCCGGGCGTGAATTAAGCCACTTACAACAACTGGAAGCCGAAAGCATTCAGATCATTCGTGAAGTCGCTGCCGAATT
CGACAATCCAGTAATGTTGTATTCCATCGGAAAAGATTCTTCGGTGATGTTACACCTTGCGCGTAAAGCTTTTTATCCCG
GCAAGATCCCATTCCCCTTGCTGCACGTGGATACGGGCTGGAAATTCAAAGAGATGATCGCTTTTCGTGATGCGCAGGCG
AAAAAGTTTGGCTTTGAATTATTAACCCACACCAACCCCGAAGGCGTTGCCCAAGGGATTAACCCCTTCGATCATGGCAG
TGCCAAGCACACTGACATCATGAAAACTCAAGGTTTAAAACAAGCCTTAAACCAATATGGTTTCGATGCCGCATTCGGTG
GTGCGCGCCGCGATGAGGAAAAGTCCCGCGCGAAGGAGCGGGTGTATTCGTTCCGCGATCGTCACCATAGATGGGATCCC
AAGAATCAGCGTCCCGAACTGTGGCGCACCTATAACGGTGCGGTAAATAAGGGCGAAAGTATTCGTGTCTTCCCGTTATC
TAACTGGACCGAACTCGATATTTGGCAATATATCTATCAAGAAAACATTGAGTTAGTACCACTGTATTTTGCCGCCGAGC
GCCCTGTGGTTGAGCGTGGCGGTCAGTTAATTATGGCCGACGATGAGCGTATGAAGCTCGAGGAAGGCGAAACCATCAAG
CATGAGGTGGTACGTTTTAGAACCTTAGGTTGTTATCCATTGACGGCGGCGATGCATTCGCAGGCGGATAATCTCGAAAA
AATCATCGAAGAAATGCTGCTAACTCGCTCGAGCGAACGCCAAGGGCGATTGATTGACTCGGATCAGAGTGCATCGATGG
AGCAGAAGAAACGCCAAGGTTATTTCTAA

Upstream 100 bases:

>100_bases
CAAGATTGTGGCAAAAATTTAGTTAAATCAGATTTTTTAGAAATAGTTAAGTTAAATCCAGCTGCCTGTCGCAGCCCATT
TTAGGTAAACGGAGAAGAGT

Downstream 100 bases:

>100_bases
ACCAAGGCATTTCGAGCGCAGGTTAGAGACAGATTAAACCGATTTCGTGAATGTCCCGATGAAGGCTCTTAGAGCAATCG
AGTACCAGTGACTTCACGAA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MAGRELSHLQQLEAESIQIIREVAAEFDNPVMLYSIGKDSSVMLHLARKAFYPGKIPFPLLHVDTGWKFKEMIAFRDAQA
KKFGFELLTHTNPEGVAQGINPFDHGSAKHTDIMKTQGLKQALNQYGFDAAFGGARRDEEKSRAKERVYSFRDRHHRWDP
KNQRPELWRTYNGAVNKGESIRVFPLSNWTELDIWQYIYQENIELVPLYFAAERPVVERGGQLIMADDERMKLEEGETIK
HEVVRFRTLGCYPLTAAMHSQADNLEKIIEEMLLTRSSERQGRLIDSDQSASMEQKKRQGYF

Sequences:

>Translated_302_residues
MAGRELSHLQQLEAESIQIIREVAAEFDNPVMLYSIGKDSSVMLHLARKAFYPGKIPFPLLHVDTGWKFKEMIAFRDAQA
KKFGFELLTHTNPEGVAQGINPFDHGSAKHTDIMKTQGLKQALNQYGFDAAFGGARRDEEKSRAKERVYSFRDRHHRWDP
KNQRPELWRTYNGAVNKGESIRVFPLSNWTELDIWQYIYQENIELVPLYFAAERPVVERGGQLIMADDERMKLEEGETIK
HEVVRFRTLGCYPLTAAMHSQADNLEKIIEEMLLTRSSERQGRLIDSDQSASMEQKKRQGYF
>Mature_301_residues
AGRELSHLQQLEAESIQIIREVAAEFDNPVMLYSIGKDSSVMLHLARKAFYPGKIPFPLLHVDTGWKFKEMIAFRDAQAK
KFGFELLTHTNPEGVAQGINPFDHGSAKHTDIMKTQGLKQALNQYGFDAAFGGARRDEEKSRAKERVYSFRDRHHRWDPK
NQRPELWRTYNGAVNKGESIRVFPLSNWTELDIWQYIYQENIELVPLYFAAERPVVERGGQLIMADDERMKLEEGETIKH
EVVRFRTLGCYPLTAAMHSQADNLEKIIEEMLLTRSSERQGRLIDSDQSASMEQKKRQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=78.4511784511784, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34938; Mature: 34807

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRELSHLQQLEAESIQIIREVAAEFDNPVMLYSIGKDSSVMLHLARKAFYPGKIPFPL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEE
LHVDTGWKFKEMIAFRDAQAKKFGFELLTHTNPEGVAQGINPFDHGSAKHTDIMKTQGLK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHH
QALNQYGFDAAFGGARRDEEKSRAKERVYSFRDRHHRWDPKNQRPELWRTYNGAVNKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCE
IRVFPLSNWTELDIWQYIYQENIELVPLYFAAERPVVERGGQLIMADDERMKLEEGETIK
EEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCEEEEECCCHHHCCCCCHHH
HEVVRFRTLGCYPLTAAMHSQADNLEKIIEEMLLTRSSERQGRLIDSDQSASMEQKKRQG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
YF
CC
>Mature Secondary Structure 
AGRELSHLQQLEAESIQIIREVAAEFDNPVMLYSIGKDSSVMLHLARKAFYPGKIPFPL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEE
LHVDTGWKFKEMIAFRDAQAKKFGFELLTHTNPEGVAQGINPFDHGSAKHTDIMKTQGLK
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHH
QALNQYGFDAAFGGARRDEEKSRAKERVYSFRDRHHRWDPKNQRPELWRTYNGAVNKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCE
IRVFPLSNWTELDIWQYIYQENIELVPLYFAAERPVVERGGQLIMADDERMKLEEGETIK
EEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCEEEEECCCHHHCCCCCHHH
HEVVRFRTLGCYPLTAAMHSQADNLEKIIEEMLLTRSSERQGRLIDSDQSASMEQKKRQG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA