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The map label for this gene is mpl [H]

Identifier: 117919243

GI number: 117919243

Start: 911715

End: 913112

Strand: Direct

Name: mpl [H]

Synonym: Shewana3_0791

Alternate gene names: 117919243

Gene position: 911715-913112 (Clockwise)

Preceding gene: 117919242

Following gene: 117919244

Centisome position: 18.34

GC content: 50.93

Gene sequence:

>1398_bases
ATGCACGTACATATTTTAGGAATTTGTGGAACCTTCATGGGTGGTCTAGCACTCTTGGCTAGGGCAAAAGGGCACAGAGT
GACAGGCTCGGATGCCAACGTCTATCCACCGATGAGTACGCAGCTTGAAGAGCAAGGTATTGAACTCATTCAAGGTTTTG
ACCAAAGCCAGTTAGGTGAAGCGGGCAGTTATCCCGATCTTGTGGTGATTGGTAATGCCATGAGCCGAGGTAATCCTTGC
GTCGAAGCGGTATTAAACCTTGGGATCCCTTACACTTCTGGACCACAGTTTTTAGCCGATCATATTCTGCCTGAGCGTTG
GGTGTTAGCCGTGTCTGGTACCCATGGAAAGACCTCCACCTCAAGCATGCTGGCGTGGATCTTAGAGGATTGTGGTTATC
AACCCGGTTTCTTGATTGGCGGCGTGCCGCAAAACTTTGGCGTGTCGGCCCGTTTAGGGGAGTCGGCGTTTTTTGTGGTC
GAAGCCGATGAGTACGACAGCGCCTTCTTCGATAAGCGTTCTAAATTTGTGCATTATCATCCCCGTACGCTGGTGATCAA
TAACCTCGAGTTTGACCATGCCGATATTTTTGCCGATTTAGCGGCGATCCAAAAACAGTTCCATCATCTGATCCGAACCG
TGCCAGGGCAAGGCAAAGTGATTTGGCCTGCCGATTCCCACGCGGTTAAGCAAACCATTGATATGGGCTGCTGGAGCGAG
CAAGAGACGTTCCACACCGCTGAGGTGACTAAGGGCTGGCATGCTAAGGCTTTAAGTGATGATTGCCATGAGTTTGAAGT
GTTCTTCGACGGCGAATCCCAAGGTGTGCTCGAATGGGCGCTGATTGGTCAGCACAATATTGAAAACGCGCTAATGGCCA
TCGCGGCCGCGCGCCATGTTGGGGTTAAACCGAGTGCTGCTATCGCGGCATTAACCCGCTTTGTACCGCCGAAACGTCGT
CTAGAGCTGTTGGGTACTGTTCACGGTGTGAGCGTATACGATGATTTTGCTCACCATCCAACGGCGATTGCGACCACCAT
TAAGGGCATGCGTGCCAAGGTCGGTCAAAGCAAAATCACTATCGTATTAGAGCCACGCTCAAACACCATGAAGAGTGGGG
TGCACAAAGACACGCTGGCTCATTCCATGCTGCAAGCGGACGAAGCCTTTTTATATCAAGCCGATACTATCGATTGGAAT
ATGAAAGAGGCGATGGAAGCGGCGGTGATCCCTGTGACTGTTCTGCATCAAATTGATGATGTTGTGGCTAAAGTCTCTGC
TTCGGCAAAAGTGGGTGACACTATCGTGGTGATGAGTAACGGCGGCTTTGGTGGCTTACATAAGAAACTGTTGGCGGCGC
TCGAAGTCAGCCCACAGTTTGTTCAGCAGGCGGACTAA

Upstream 100 bases:

>100_bases
ATCTTTGCCAAACAAGTGGCTTTTCCGAGATCCTAACCTGCCAATCTGCGGTGCTATGGATTAGAATAGCCTTGAATTCA
AAAACATCAGAGCGAGCGGT

Downstream 100 bases:

>100_bases
ATATGGCCTACGCCAAATCTGAGAAAGCCATTAGCTTAGCCTGGACGGGGGCCTCGGGCGCGCCCTATGGCTTGAAATTA
TTAGAATGTTTATTAGCCTC

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEEQGIELIQGFDQSQLGEAGSYPDLVVIGNAMSRGNPC
VEAVLNLGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILEDCGYQPGFLIGGVPQNFGVSARLGESAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFHHLIRTVPGQGKVIWPADSHAVKQTIDMGCWSE
QETFHTAEVTKGWHAKALSDDCHEFEVFFDGESQGVLEWALIGQHNIENALMAIAAARHVGVKPSAAIAALTRFVPPKRR
LELLGTVHGVSVYDDFAHHPTAIATTIKGMRAKVGQSKITIVLEPRSNTMKSGVHKDTLAHSMLQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVSASAKVGDTIVVMSNGGFGGLHKKLLAALEVSPQFVQQAD

Sequences:

>Translated_465_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEEQGIELIQGFDQSQLGEAGSYPDLVVIGNAMSRGNPC
VEAVLNLGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILEDCGYQPGFLIGGVPQNFGVSARLGESAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFHHLIRTVPGQGKVIWPADSHAVKQTIDMGCWSE
QETFHTAEVTKGWHAKALSDDCHEFEVFFDGESQGVLEWALIGQHNIENALMAIAAARHVGVKPSAAIAALTRFVPPKRR
LELLGTVHGVSVYDDFAHHPTAIATTIKGMRAKVGQSKITIVLEPRSNTMKSGVHKDTLAHSMLQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVSASAKVGDTIVVMSNGGFGGLHKKLLAALEVSPQFVQQAD
>Mature_465_residues
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEEQGIELIQGFDQSQLGEAGSYPDLVVIGNAMSRGNPC
VEAVLNLGIPYTSGPQFLADHILPERWVLAVSGTHGKTSTSSMLAWILEDCGYQPGFLIGGVPQNFGVSARLGESAFFVV
EADEYDSAFFDKRSKFVHYHPRTLVINNLEFDHADIFADLAAIQKQFHHLIRTVPGQGKVIWPADSHAVKQTIDMGCWSE
QETFHTAEVTKGWHAKALSDDCHEFEVFFDGESQGVLEWALIGQHNIENALMAIAAARHVGVKPSAAIAALTRFVPPKRR
LELLGTVHGVSVYDDFAHHPTAIATTIKGMRAKVGQSKITIVLEPRSNTMKSGVHKDTLAHSMLQADEAFLYQADTIDWN
MKEAMEAAVIPVTVLHQIDDVVAKVSASAKVGDTIVVMSNGGFGGLHKKLLAALEVSPQFVQQAD

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=463, Percent_Identity=63.2829373650108, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1786279, Length=404, Percent_Identity=26.4851485148515, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50493; Mature: 50493

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEEQGIELIQGFDQSQLGE
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
AGSYPDLVVIGNAMSRGNPCVEAVLNLGIPYTSGPQFLADHILPERWVLAVSGTHGKTST
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCH
SSMLAWILEDCGYQPGFLIGGVPQNFGVSARLGESAFFVVEADEYDSAFFDKRSKFVHYH
HHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHCCCCEEEEC
PRTLVINNLEFDHADIFADLAAIQKQFHHLIRTVPGQGKVIWPADSHAVKQTIDMGCWSE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCC
QETFHTAEVTKGWHAKALSDDCHEFEVFFDGESQGVLEWALIGQHNIENALMAIAAARHV
CCCEEHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GVKPSAAIAALTRFVPPKRRLELLGTVHGVSVYDDFAHHPTAIATTIKGMRAKVGQSKIT
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
IVLEPRSNTMKSGVHKDTLAHSMLQADEAFLYQADTIDWNMKEAMEAAVIPVTVLHQIDD
EEECCCCCHHHCCCHHHHHHHHHHHHCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
VVAKVSASAKVGDTIVVMSNGGFGGLHKKLLAALEVSPQFVQQAD
HHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MHVHILGICGTFMGGLALLARAKGHRVTGSDANVYPPMSTQLEEQGIELIQGFDQSQLGE
CEEEEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
AGSYPDLVVIGNAMSRGNPCVEAVLNLGIPYTSGPQFLADHILPERWVLAVSGTHGKTST
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCH
SSMLAWILEDCGYQPGFLIGGVPQNFGVSARLGESAFFVVEADEYDSAFFDKRSKFVHYH
HHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCEEEEEECCCCCHHHHHCCCCEEEEC
PRTLVINNLEFDHADIFADLAAIQKQFHHLIRTVPGQGKVIWPADSHAVKQTIDMGCWSE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCC
QETFHTAEVTKGWHAKALSDDCHEFEVFFDGESQGVLEWALIGQHNIENALMAIAAARHV
CCCEEHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GVKPSAAIAALTRFVPPKRRLELLGTVHGVSVYDDFAHHPTAIATTIKGMRAKVGQSKIT
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
IVLEPRSNTMKSGVHKDTLAHSMLQADEAFLYQADTIDWNMKEAMEAAVIPVTVLHQIDD
EEECCCCCHHHCCCHHHHHHHHHHHHCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
VVAKVSASAKVGDTIVVMSNGGFGGLHKKLLAALEVSPQFVQQAD
HHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]