Click here to switch to the map view.

The map label for this gene is nudF [H]

Identifier: 117919178

GI number: 117919178

Start: 843080

End: 843691

Strand: Direct

Name: nudF [H]

Synonym: Shewana3_0726

Alternate gene names: 117919178

Gene position: 843080-843691 (Clockwise)

Preceding gene: 117919176

Following gene: 117919179

Centisome position: 16.96

GC content: 47.88

Gene sequence:

>612_bases
ATGAAGCCTGCTGGTTTTTCGCAAACAGATGTGGAAATTCTCGAGAAAAAGTCGCTGTATCAAGGTTTTTTCGCCCTCGA
ACAGTTCACTTTTAAACACAAATTATTTGCTGGTGGTTGGAGCCAACCCGTGACCCGTGAAGTGTTCGAGCGTGGCCATG
CCGTGGTGGTTCTGCCCTATGATGCTGCGCGTGACAAGATTGTTTTAATTGAACAAATTCGTTTTCCTGCATTAGCGACC
ACTAAATCTCCTTGGCTAATGGAGTTGGTCGCGGGTATGATTGCGCCCGACGAAACGCCGCAGGATGTTGCGCATCGCGA
ACTGCTGGAAGAAACCGGCTTAACCGCCCGCAATATGCATTATGTGAATAGTTATCTGGCAAGTCCTGGTGGCAGTACGG
AGCGTTTCTATTTCTATTGGGCCGAGGTGGATTCATCCCTGGCACAAGGTCTGCACGGATTAGCTGACGAACACGAAGAT
ATTCGATTGCATGTGTTAGACCGTGAGGATGCCTATAATCAGGTCGTCAACGGGGCAATAGACAATGCGTCGACCGTAAT
TGGATTGCAGTGGTTGCAATTAAACTATCAGCAACTGGTTAAAGTTGGATAA

Upstream 100 bases:

>100_bases
CACACGTTCAAGTCAACTGCGAAGTGTAACAGATTTTATTTTAAAAATGAGCCAAATTGCCTTGATAATAGGCGAAAATT
TAACGCTTAAGGATTGAAAT

Downstream 100 bases:

>100_bases
AGGTTTGGCTACTTCAACATCGCACCGAAAACCGCGCTACCAACCTAACGTGAGCGACTTTTTAGCGCTATGCGGGCGTA
ACTACGGCTATATGCAGAAG

Product: nucleoside diphosphate pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKPAGFSQTDVEILEKKSLYQGFFALEQFTFKHKLFAGGWSQPVTREVFERGHAVVVLPYDAARDKIVLIEQIRFPALAT
TKSPWLMELVAGMIAPDETPQDVAHRELLEETGLTARNMHYVNSYLASPGGSTERFYFYWAEVDSSLAQGLHGLADEHED
IRLHVLDREDAYNQVVNGAIDNASTVIGLQWLQLNYQQLVKVG

Sequences:

>Translated_203_residues
MKPAGFSQTDVEILEKKSLYQGFFALEQFTFKHKLFAGGWSQPVTREVFERGHAVVVLPYDAARDKIVLIEQIRFPALAT
TKSPWLMELVAGMIAPDETPQDVAHRELLEETGLTARNMHYVNSYLASPGGSTERFYFYWAEVDSSLAQGLHGLADEHED
IRLHVLDREDAYNQVVNGAIDNASTVIGLQWLQLNYQQLVKVG
>Mature_203_residues
MKPAGFSQTDVEILEKKSLYQGFFALEQFTFKHKLFAGGWSQPVTREVFERGHAVVVLPYDAARDKIVLIEQIRFPALAT
TKSPWLMELVAGMIAPDETPQDVAHRELLEETGLTARNMHYVNSYLASPGGSTERFYFYWAEVDSSLAQGLHGLADEHED
IRLHVLDREDAYNQVVNGAIDNASTVIGLQWLQLNYQQLVKVG

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=197, Percent_Identity=54.8223350253807, Blast_Score=231, Evalue=3e-62,
Organism=Escherichia coli, GI1788810, Length=188, Percent_Identity=29.7872340425532, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 22898; Mature: 22898

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAGFSQTDVEILEKKSLYQGFFALEQFTFKHKLFAGGWSQPVTREVFERGHAVVVLPY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEC
DAARDKIVLIEQIRFPALATTKSPWLMELVAGMIAPDETPQDVAHRELLEETGLTARNMH
CCCCCCEEEEECCCCCCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
YVNSYLASPGGSTERFYFYWAEVDSSLAQGLHGLADEHEDIRLHVLDREDAYNQVVNGAI
HHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCC
DNASTVIGLQWLQLNYQQLVKVG
CCCHHHEEHHHHHHCHHHHHCCC
>Mature Secondary Structure
MKPAGFSQTDVEILEKKSLYQGFFALEQFTFKHKLFAGGWSQPVTREVFERGHAVVVLPY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEC
DAARDKIVLIEQIRFPALATTKSPWLMELVAGMIAPDETPQDVAHRELLEETGLTARNMH
CCCCCCEEEEECCCCCCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
YVNSYLASPGGSTERFYFYWAEVDSSLAQGLHGLADEHEDIRLHVLDREDAYNQVVNGAI
HHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCC
DNASTVIGLQWLQLNYQQLVKVG
CCCHHHEEHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]