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The map label for this gene is rlmB [H]

Identifier: 116689569

GI number: 116689569

Start: 1713954

End: 1714697

Strand: Direct

Name: rlmB [H]

Synonym: Bcen2424_1547

Alternate gene names: 116689569

Gene position: 1713954-1714697 (Clockwise)

Preceding gene: 116689568

Following gene: 116689570

Centisome position: 49.2

GC content: 68.82

Gene sequence:

>744_bases
ATGTCACGTCTGAAGGTTCTTTACGGTTTTCATGCGGTGACCGCACGTTTGCGGCACGATGCATCGACGGTGGCGGAGGT
GCTGTACGACCAGACGCGCCGCGACCGCCGCATGCAGGACTTCCTGCACGCCGCGAAGGAAGCGGGCGTGCGGCTGATCG
CAGCCGACGAAACGCGCCTGTGGGGCCTCGCGCACACCGAGCGCCATCAGGGTGTCGTCGCGCGCGTCGAGGACATGCCG
CTTGCGCAGAACCTCTCCGAGCTGCTCGACGGCATCAACGGGCCCGCGCTGCTGCTCGTGCTCGACGGCGTCACCGATCC
GCACAACCTCGGCGCATGCCTGCGGGTCGCCGATTCAGCCGGCGCGCATGCGGTGATCGCCCCGCGTGACCGCGCGGTCG
GCCTGAACGCGACCGCGGCCAAGGTGGCGAGCGGCGCGGCCGACACGGTGCCGTACATCACGGTGACGAACCTCGCCCGC
GCGCTGCGCGAGCTGAAGGAAGCGGGGGTATGGATCATCGGCACGTCGGACGAGGCGTCGGCGACGCTCTACGAAACGAA
GCTCGACGGCCCGGTCGCGCTCGTGATGGGCGCGGAAGGCGAAGGCATGCGCCGGCTCACGCGCGATACCTGCGACGAAG
TGATGAGCATCCCGATGGCCGGCAGCGTGGAAAGCCTGAACGTGTCGGTCGCCAGCGGTGTCTGCCTGTACGAAGCCGTG
CGTCAGCGGCGCGTGAAGGGCTGA

Upstream 100 bases:

>100_bases
CGTCACCGGTCATCGGCCGGCCACGCGGCGCTTTCCTTTTCCATGGATCGCGGCCGCGCCCGTCGCGCGGCCGCACGTTT
GATCGAAGGTTGTTCCAGTC

Downstream 100 bases:

>100_bases
ACCTTCGGGCTGAACCCCGGGGCTGACCCGGGGCCGACCCGCCCGTCCCGCGCGCGATGCGCGGGCGTCGAATGACCCGC
ACGACGCGTGCCCCGATGCA

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDMP
LAQNLSELLDGINGPALLLVLDGVTDPHNLGACLRVADSAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKEAGVWIIGTSDEASATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
RQRRVKG

Sequences:

>Translated_247_residues
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDMP
LAQNLSELLDGINGPALLLVLDGVTDPHNLGACLRVADSAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKEAGVWIIGTSDEASATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
RQRRVKG
>Mature_246_residues
SRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDMPL
AQNLSELLDGINGPALLLVLDGVTDPHNLGACLRVADSAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARA
LRELKEAGVWIIGTSDEASATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAVR
QRRVKG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=26.9961977186312, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=55.1867219917012, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788935, Length=240, Percent_Identity=28.75, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790083, Length=157, Percent_Identity=36.9426751592357, Blast_Score=81, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17555908, Length=256, Percent_Identity=25.390625, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=162, Percent_Identity=31.4814814814815, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26477; Mature: 26345

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDMPLAQNLSELLDGINGPALLLVLDGVTDPHNLGACLRVADSA
EECCHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHEECCCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKEAGVWIIGTSDEAS
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
EEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHCEEHHHCCHHHHHHH
RQRRVKG
HHHCCCC
>Mature Secondary Structure 
SRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHAAKEAGVRLIAADETRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDMPLAQNLSELLDGINGPALLLVLDGVTDPHNLGACLRVADSA
EECCHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHEECCCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKEAGVWIIGTSDEAS
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
EEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHCEEHHHCCHHHHHHH
RQRRVKG
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]