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The map label for this gene is amiD [H]

Identifier: 116689570

GI number: 116689570

Start: 1714831

End: 1715703

Strand: Direct

Name: amiD [H]

Synonym: Bcen2424_1548

Alternate gene names: 116689570

Gene position: 1714831-1715703 (Clockwise)

Preceding gene: 116689569

Following gene: 116689571

Centisome position: 49.22

GC content: 69.64

Gene sequence:

>873_bases
ATGACCCTGTTTCGTATTGGCGCCTGCTGCGCCGTGTTCGGCCTGCTGGCGGCCTGCACGTCGCCGTCGCTCGTCGAACG
CGGCACCTACTACGCCGACACGAGCCTGCACGCGCGCGGCGCCGATTCGCGGATTCGCTTTCTCGTCATGCATTACACCG
AAAGCGACGAGACGAAATCGCTGCGCACGCTGACGGGCGACTCGGTCAGCGTGCATTACGTCATCCCGCCGCAGCCGCGC
GCCGAACGCGGGATGCCCGTCATCTACCAGCTCGTCCCGGAAGCGCGGCGCGCGTGGCACGCGGGCGTCAGCGAGTGGCA
GGGCACGACCGAGCTGAACGCGGTGTCGATCGGCATCGAGAACGTGAACCGCGGCCCGCTCGATCCGCAGCACCGCACCT
GGCAGCCATATCCGCCCGAACAGGTCGACGCGCTGACCCGCCTGTCGAAGGACATCGTCGCGCGCTACGGCATTCCGCCG
ACGCGCGTGGTCGGGCACAGCGATATCGCGCCTCAACGCAAGATCGATCCCGGCCCGCTGTTTCCTTGGCATGCGCTCGC
GCAAGCCGGCGTCGGCGCATGGCCGGACGACGCGACCGTCGCCGCTCGGCTCGCCGGCCGCGACCCGCACGCGCCGGTCG
ACGTGCGCGAATTGCAGCTCAAGCTCGCGCGCTACGGCTACGACGTGCCGACCGACGGCGTGCTCGACACGCGTACGCGG
CGCGTGTTCGCGGCGTTCCAGATGCATTTCCGGCCGTCCGATTACGCGGGCAATCCGGACGCGGAAAGCGATGCGATCGC
GCAGGCGCTGCTCGACAAGTACTTCCCCGGCACGCAAGCGGCGGACCCCGCGCCGGCGGCCGACACACCCTGA

Upstream 100 bases:

>100_bases
CCGACCCGCCCGTCCCGCGCGCGATGCGCGGGCGTCGAATGACCCGCACGACGCGTGCCCCGATGCAAGGCGGCACGCGG
CTCTCCGGAACCCGCGACCG

Downstream 100 bases:

>100_bases
ATACGCGGCGCGGCGCACGCCGAAAAACGCGGGGACGTCCGCCGACTCCGGTAAACTGGCGGTTTTCCGCAATCCCGCAG
CATTACCACGCCATGACTCA

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTLFRIGACCAVFGLLAACTSPSLVERGTYYADTSLHARGADSRIRFLVMHYTESDETKSLRTLTGDSVSVHYVIPPQPR
AERGMPVIYQLVPEARRAWHAGVSEWQGTTELNAVSIGIENVNRGPLDPQHRTWQPYPPEQVDALTRLSKDIVARYGIPP
TRVVGHSDIAPQRKIDPGPLFPWHALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQLKLARYGYDVPTDGVLDTRTR
RVFAAFQMHFRPSDYAGNPDAESDAIAQALLDKYFPGTQAADPAPAADTP

Sequences:

>Translated_290_residues
MTLFRIGACCAVFGLLAACTSPSLVERGTYYADTSLHARGADSRIRFLVMHYTESDETKSLRTLTGDSVSVHYVIPPQPR
AERGMPVIYQLVPEARRAWHAGVSEWQGTTELNAVSIGIENVNRGPLDPQHRTWQPYPPEQVDALTRLSKDIVARYGIPP
TRVVGHSDIAPQRKIDPGPLFPWHALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQLKLARYGYDVPTDGVLDTRTR
RVFAAFQMHFRPSDYAGNPDAESDAIAQALLDKYFPGTQAADPAPAADTP
>Mature_289_residues
TLFRIGACCAVFGLLAACTSPSLVERGTYYADTSLHARGADSRIRFLVMHYTESDETKSLRTLTGDSVSVHYVIPPQPRA
ERGMPVIYQLVPEARRAWHAGVSEWQGTTELNAVSIGIENVNRGPLDPQHRTWQPYPPEQVDALTRLSKDIVARYGIPPT
RVVGHSDIAPQRKIDPGPLFPWHALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQLKLARYGYDVPTDGVLDTRTRR
VFAAFQMHFRPSDYAGNPDAESDAIAQALLDKYFPGTQAADPAPAADTP

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=252, Percent_Identity=54.7619047619048, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI1786300, Length=96, Percent_Identity=40.625, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31774; Mature: 31643

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFRIGACCAVFGLLAACTSPSLVERGTYYADTSLHARGADSRIRFLVMHYTESDETKS
CCEEHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCCCCCEEEEEEEEECCCCCHHH
LRTLTGDSVSVHYVIPPQPRAERGMPVIYQLVPEARRAWHAGVSEWQGTTELNAVSIGIE
HHEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCEEEEEECC
NVNRGPLDPQHRTWQPYPPEQVDALTRLSKDIVARYGIPPTRVVGHSDIAPQRKIDPGPL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCCCCCCC
FPWHALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQLKLARYGYDVPTDGVLDTRTR
CHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
RVFAAFQMHFRPSDYAGNPDAESDAIAQALLDKYFPGTQAADPAPAADTP
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLFRIGACCAVFGLLAACTSPSLVERGTYYADTSLHARGADSRIRFLVMHYTESDETKS
CEEHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCCCCCEEEEEEEEECCCCCHHH
LRTLTGDSVSVHYVIPPQPRAERGMPVIYQLVPEARRAWHAGVSEWQGTTELNAVSIGIE
HHEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCEEEEEECC
NVNRGPLDPQHRTWQPYPPEQVDALTRLSKDIVARYGIPPTRVVGHSDIAPQRKIDPGPL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCCCCCCC
FPWHALAQAGVGAWPDDATVAARLAGRDPHAPVDVRELQLKLARYGYDVPTDGVLDTRTR
CHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
RVFAAFQMHFRPSDYAGNPDAESDAIAQALLDKYFPGTQAADPAPAADTP
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]