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The map label for this gene is pilR [H]

Identifier: 116689551

GI number: 116689551

Start: 1690970

End: 1692361

Strand: Direct

Name: pilR [H]

Synonym: Bcen2424_1529

Alternate gene names: 116689551

Gene position: 1690970-1692361 (Clockwise)

Preceding gene: 116689550

Following gene: 116689552

Centisome position: 48.54

GC content: 65.23

Gene sequence:

>1392_bases
ATGAGAAATACGCCCGCAATCGAAGAGCTCGACCTGTACGTCTGGGAAGGCAAGGCGGACATCGTCGACCGCGTCGCCCG
GTGCATGGCGAGCTTCGACGTCGAAGTGATCCGCGCCGACAACGCGGCGGTCTCGCCCGAGCGCGCCGCATTGCGGCGGT
CGCTGGCGATCATCAGCGTGACGATGATCGAGGGCGGCGCGGCATTCCTGCGCGACTGGCAGGCCAACATCGGCATGCCG
GTGGTCTGGGTCGGCGCGGCGCGCGACCACGACGCGTCGCAGTATCCGCCCGAGTATTCGCACATCCTGCCGCTCGACTT
CACCTGTGCCGAACTGCGCGGCATGATCGGCAAGCTCGTCACGCAACTGCGGGCGCACGCAGCCGAAACGTTGCAGCCGT
CGGAACTCGTCGCGCACTCCGAATCGATGCAGGCGCTGCTGCACGAAGTCGATACGTTCGCCGACTGCGACACCAACGTG
CTGCTGCACGGTGAAACCGGCGTCGGCAAGGAGCGCATCGCGCAACTGCTGCACGAAAAGCATTCGCGCTATCGGCACGG
CGAATTCGTGCCGGTGAACTGCGGCGCGATTCCGGACGGCCTGTTCGAATCGCTGTTCTTCGGTCATGCGAAAGGGTCGT
TCACGGGCGCGGTTGTTGCGCATAAAGGCTATTTCGAGCAGGCGGCCGGCGGCACGCTGTTCCTCGACGAGGTCGGCGAT
CTGCCGCTGTACCAGCAGGTCAAGCTGCTGCGCGTGCTCGAGGACGGCGCGGTGCTGCGGGTCGGTGCGACCGCGCCCGT
CAAGGTCGATTTCCGTCTGGTCGCGGCGAGCAACAAGAAGCTGCCGCAGCTCGTGAAGGAAGGGTTGTTCCGCGCCGATC
TCTATTACCGGCTCGCGGTGATCGAACTGAGCATTCCGTCGCTAGAAGAGCGCGGTGCGGTGGACAAGATCGCGCTGTTC
AAGTCGTTCGTCGCCCAGGTCGTGGGCGAGGAGCGGCTCGCCCAGTTGTCCGATCTGCCGTACTGGCTCACGGATTCGGT
CGCGGACAGCTATTTCCCCGGCAACGTGCGCGAACTGCGCAACCTGGCCGAGCGGGTCGGCGTGACGGTACGGCAGACGG
GCGGCTGGGATGCCGCGCGGCTGCAGCGGCTGATCGCGCATGCGCGCAGCGCGGCGCAGCCGGTGCCGGCGGAGAGCGCG
GCCGAGGTGTTCGTCGATCGCAGCAAGTGGGACATGAACGAGCGCAACCGCGTGATCTCGGCGCTCGATGCAAACGGTTG
GCGGCGTCAGGACACGGCCCAGCAACTCGGCATCAGCCGCAAGGTATTGTGGGAAAAAATGCGCAAATATCAGATCTTCG
ACGAAGAGCCCGAGACCCGCGAAAGTGAGTAA

Upstream 100 bases:

>100_bases
CTGCTGAATCTGCTTGGCGTCCAGGTCGGCGCGGCGACGGTCCATCAGATCTCGCTCAATTGCGGGGTCGCCCAAACGGT
TTACTGAATAAGCAGATCAG

Downstream 100 bases:

>100_bases
TTAATGAGATAAAATCGCGGTGAATTACAACGACTCGGGCGGGAATAAAACTTCATGAGCCATATGACGGGGTTGGGGCG
GGTGAGCGTATTGCTGCTCG

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRNTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAAVSPERAALRRSLAIISVTMIEGGAAFLRDWQANIGMP
VVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMIGKLVTQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNV
LLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
LPLYQQVKLLRVLEDGAVLRVGATAPVKVDFRLVAASNKKLPQLVKEGLFRADLYYRLAVIELSIPSLEERGAVDKIALF
KSFVAQVVGEERLAQLSDLPYWLTDSVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAESA
AEVFVDRSKWDMNERNRVISALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE

Sequences:

>Translated_463_residues
MRNTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAAVSPERAALRRSLAIISVTMIEGGAAFLRDWQANIGMP
VVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMIGKLVTQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNV
LLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
LPLYQQVKLLRVLEDGAVLRVGATAPVKVDFRLVAASNKKLPQLVKEGLFRADLYYRLAVIELSIPSLEERGAVDKIALF
KSFVAQVVGEERLAQLSDLPYWLTDSVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAESA
AEVFVDRSKWDMNERNRVISALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE
>Mature_463_residues
MRNTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAAVSPERAALRRSLAIISVTMIEGGAAFLRDWQANIGMP
VVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMIGKLVTQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNV
LLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
LPLYQQVKLLRVLEDGAVLRVGATAPVKVDFRLVAASNKKLPQLVKEGLFRADLYYRLAVIELSIPSLEERGAVDKIALF
KSFVAQVVGEERLAQLSDLPYWLTDSVADSYFPGNVRELRNLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAESA
AEVFVDRSKWDMNERNRVISALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=336, Percent_Identity=36.6071428571429, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1790437, Length=352, Percent_Identity=35.5113636363636, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788550, Length=337, Percent_Identity=35.0148367952522, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1790299, Length=423, Percent_Identity=33.3333333333333, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=36.9565217391304, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789233, Length=335, Percent_Identity=33.4328358208955, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87082152, Length=254, Percent_Identity=42.1259842519685, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=35.4938271604938, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1788905, Length=230, Percent_Identity=40, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=35.8208955223881, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1787583, Length=353, Percent_Identity=33.4277620396601, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789828, Length=154, Percent_Identity=40.9090909090909, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081858, Length=301, Percent_Identity=28.9036544850498, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51503; Mature: 51503

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAAVSPERAALRRSLAIISV
CCCCCCCHHCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHEEE
TMIEGGAAFLRDWQANIGMPVVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMIGKLV
EEECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNVLLHGETGVGKERIAQLLHEK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHH
HSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
HHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCEEEECCCCC
LPLYQQVKLLRVLEDGAVLRVGATAPVKVDFRLVAASNKKLPQLVKEGLFRADLYYRLAV
CCHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEE
IELSIPSLEERGAVDKIALFKSFVAQVVGEERLAQLSDLPYWLTDSVADSYFPGNVRELR
EEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHH
NLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAESAAEVFVDRSKWDMNERNRVIS
HHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
ALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE
HHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNTPAIEELDLYVWEGKADIVDRVARCMASFDVEVIRADNAAVSPERAALRRSLAIISV
CCCCCCCHHCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHEEE
TMIEGGAAFLRDWQANIGMPVVWVGAARDHDASQYPPEYSHILPLDFTCAELRGMIGKLV
EEECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TQLRAHAAETLQPSELVAHSESMQALLHEVDTFADCDTNVLLHGETGVGKERIAQLLHEK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHH
HSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVGD
HHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCEEEECCCCC
LPLYQQVKLLRVLEDGAVLRVGATAPVKVDFRLVAASNKKLPQLVKEGLFRADLYYRLAV
CCHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEE
IELSIPSLEERGAVDKIALFKSFVAQVVGEERLAQLSDLPYWLTDSVADSYFPGNVRELR
EEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHH
NLAERVGVTVRQTGGWDAARLQRLIAHARSAAQPVPAESAAEVFVDRSKWDMNERNRVIS
HHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHH
ALDANGWRRQDTAQQLGISRKVLWEKMRKYQIFDEEPETRESE
HHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]