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The map label for this gene is truB [H]

Identifier: 116689524

GI number: 116689524

Start: 1657301

End: 1658233

Strand: Direct

Name: truB [H]

Synonym: Bcen2424_1502

Alternate gene names: 116689524

Gene position: 1657301-1658233 (Clockwise)

Preceding gene: 116689523

Following gene: 116689529

Centisome position: 47.57

GC content: 67.85

Gene sequence:

>933_bases
ATGACGAATGCAGCACCCCCACGTCCGCGCGTGCCCCGGCGCGTGCTGGACGGCGTTCTCCTGCTCGACAAGCCGATCGG
CCTGTCGAGCAACGATGCGCTGATTCGCGCAAAGCGCCTGCTTCTCGCGAAGAAAGCCGGTCACACCGGCACGCTCGATC
CGCTGGCTTCGGGCCTGCTGCCGCTGTGCTTCGGCGAGGCGACGAAGTTCTCGCAGGACTTGCTCGACGCCGACAAGACG
TATGAAGCGACGATGCGTCTCGGCCAGCGTACGGCCACCGGTGATGCGGAAGGCGAGGTGATCGACACGCGGCCCGTCGA
ATGCGATCGCGCGGCGGTGGAGGCCGCGCTCGTGCGCTTCACCGGCGACATCGTGCAGGTACCGCCGATGTATTCGGCGC
TCAAACGCGACGGCAAGCCGCTGTACGAATATGCGCGGGCCGGCCAGACCGTCGAGCGGGAGGGTCGCAACGTGACGATC
CATGCGCTCGATCTGCTCGCTTGCGAGCTGCCCGACGTGACGTTTCGCGTGACCTGCAGCAAGGGCACCTACGTGCGTAC
GCTCGCGGAAGATATCGGTGAAGCGCTCGGCTGCGGTGGGCATCTGACGATGCTGCGCCGTACGGGCGTCGGCGCGCTGA
CGCTCGAGCATGCGGTGACGCTCGATGCGCTGTCGGACGCTGCCGAAGCGGCGCGCGATGCATGGCTTCAGCCGGTCGAT
GCGCTGCTGTCGACGTTTCCGCTGGTGCGTCTCGACGAAGCCAGCGCGAAGCGGTTCCTGCACGGCCAGCGTCTACCGCT
GTCGGCCCTCGAGGCGATCGACGCGGCCGATGGCGAGCGCGTGCGCGTGTACGACGCCACCCGGTTGCTGGGCGTGGCGC
GCAAGGCGAATGGCGTGCTCGCCCCGGAACGGCTCGTCGTGACGGCTCAATGA

Upstream 100 bases:

>100_bases
GTCGAAATGTCGCGCCTGATCAAGGAAGCGAACTCGACGCGCGCGAAGGACGACGACGAGGCCGACACGCCTGCCAAGGA
CGATTGAGCTCGACCGGCCT

Downstream 100 bases:

>100_bases
CGCAGCAACGCACGCAAGTGCGCGTGGCAGAATGCAAAAAGCCCGGTCGATTTCGACCGGGCTTTTTATTTGGCGTGTTG
CGTTTAACGTGGCCGCTCAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTNAAPPRPRVPRRVLDGVLLLDKPIGLSSNDALIRAKRLLLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLDADKT
YEATMRLGQRTATGDAEGEVIDTRPVECDRAAVEAALVRFTGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTI
HALDLLACELPDVTFRVTCSKGTYVRTLAEDIGEALGCGGHLTMLRRTGVGALTLEHAVTLDALSDAAEAARDAWLQPVD
ALLSTFPLVRLDEASAKRFLHGQRLPLSALEAIDAADGERVRVYDATRLLGVARKANGVLAPERLVVTAQ

Sequences:

>Translated_310_residues
MTNAAPPRPRVPRRVLDGVLLLDKPIGLSSNDALIRAKRLLLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLDADKT
YEATMRLGQRTATGDAEGEVIDTRPVECDRAAVEAALVRFTGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTI
HALDLLACELPDVTFRVTCSKGTYVRTLAEDIGEALGCGGHLTMLRRTGVGALTLEHAVTLDALSDAAEAARDAWLQPVD
ALLSTFPLVRLDEASAKRFLHGQRLPLSALEAIDAADGERVRVYDATRLLGVARKANGVLAPERLVVTAQ
>Mature_309_residues
TNAAPPRPRVPRRVLDGVLLLDKPIGLSSNDALIRAKRLLLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLDADKTY
EATMRLGQRTATGDAEGEVIDTRPVECDRAAVEAALVRFTGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTIH
ALDLLACELPDVTFRVTCSKGTYVRTLAEDIGEALGCGGHLTMLRRTGVGALTLEHAVTLDALSDAAEAARDAWLQPVDA
LLSTFPLVRLDEASAKRFLHGQRLPLSALEAIDAADGERVRVYDATRLLGVARKANGVLAPERLVVTAQ

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=226, Percent_Identity=37.6106194690265, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI4503337, Length=262, Percent_Identity=27.4809160305344, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI215599015, Length=261, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI2367200, Length=316, Percent_Identity=45.8860759493671, Blast_Score=257, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17553978, Length=271, Percent_Identity=28.0442804428044, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=299, Percent_Identity=28.0936454849498, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=199, Percent_Identity=33.1658291457286, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364189, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364187, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364185, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364183, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI62471759, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17975520, Length=260, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33340; Mature: 33208

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAAPPRPRVPRRVLDGVLLLDKPIGLSSNDALIRAKRLLLAKKAGHTGTLDPLASGLL
CCCCCCCCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCH
PLCFGEATKFSQDLLDADKTYEATMRLGQRTATGDAEGEVIDTRPVECDRAAVEAALVRF
HHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHH
TGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTIHALDLLACELPDVTFRVTCS
CCCEEECCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEEEEEHHHCCCCCEEEEEEC
KGTYVRTLAEDIGEALGCGGHLTMLRRTGVGALTLEHAVTLDALSDAAEAARDAWLQPVD
CCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLSTFPLVRLDEASAKRFLHGQRLPLSALEAIDAADGERVRVYDATRLLGVARKANGVL
HHHHHCCCEEECHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCC
APERLVVTAQ
CCCEEEEEEC
>Mature Secondary Structure 
TNAAPPRPRVPRRVLDGVLLLDKPIGLSSNDALIRAKRLLLAKKAGHTGTLDPLASGLL
CCCCCCCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCH
PLCFGEATKFSQDLLDADKTYEATMRLGQRTATGDAEGEVIDTRPVECDRAAVEAALVRF
HHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHH
TGDIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTIHALDLLACELPDVTFRVTCS
CCCEEECCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEEEEEHHHCCCCCEEEEEEC
KGTYVRTLAEDIGEALGCGGHLTMLRRTGVGALTLEHAVTLDALSDAAEAARDAWLQPVD
CCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLSTFPLVRLDEASAKRFLHGQRLPLSALEAIDAADGERVRVYDATRLLGVARKANGVL
HHHHHCCCEEECHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCC
APERLVVTAQ
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA