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The map label for this gene is yafC [H]

Identifier: 116689416

GI number: 116689416

Start: 1538276

End: 1539208

Strand: Reverse

Name: yafC [H]

Synonym: Bcen2424_1394

Alternate gene names: 116689416

Gene position: 1539208-1538276 (Counterclockwise)

Preceding gene: 116689423

Following gene: 116689414

Centisome position: 44.18

GC content: 68.92

Gene sequence:

>933_bases
ATGCTGACCATGCAACATCTTCCTGATCTCGAAGCCTGGGCCATCTTCGCGCGCGTCGCGGAGACCGGTTCGTTCGCGAA
AGCGGCCGACCTGCTCGGCATCTCGCAACCCACCGTGTCGAAGGCGATCGCACGCCTCGAAAAACGCCTCGGCGCGATGC
TGCTGTACCGCACGTCGCGCAAGCTGTCGCTCACGCCGACCGGCGAACTGCTGCGCGACCGCGCGATCCGCCTGCTCGCC
GATGCGGAGCTGATCGAGAACGAAGCGTCCGCGCAGGCGCTCGAGCCGCACGGCCTCGTGCGCGTGAATGCGCCGATGTC
GTTCGGGCTGCACCATCTGTCGCCGGTGCTGCCCGCGTTTCTCGAGCGCTATCCGCGCGTCGACATCGATCTCGTGCTCA
CCGACCACATCGTCGACATCGTGTCGGGCGGCTTCGACGTCGCGATCCGCATCGCCGAGCTGAGCGATTCGACGCTGCGC
TCGCGCCGCCTGTGCGCGGTGCGCCGGCCGCTCGTCGCGTCGCCCGCGTATCTCGATCGGCGCGGCCGCCCGACGCATCC
GCGCGACCTCGAACAGCACGTGTGCCTCACGTACACCAACCTGCCGACGCCCGAGTACTGGCGCTTCCGTCATCCGGCGT
CGGGCGACGAAGTCGGCGTGTCCGTGCACGGGCGCATCCGCACCAACAACGCGGACGTGATCCTCCCGGCCCTCGTCGGC
GGTCACGGCCTCGCGCTGCAGCCCGAGTTCCTCGTGTGGGACGAACTGCAGCGCGGCGCGCTCGAGGAAGTGATGAGCGA
CTGGAAGATCGCGGACATCAACGTGAACCTCGTCACGCCGCCCGGCATGCTGCGCGCCGCGCGCGTGACGGTGCTGCTCG
ATTTCCTCGCCGAACATTTCTCGCACGCGCCGTGGGCGCTGCCCGCCGCCTGA

Upstream 100 bases:

>100_bases
TAAGATTCCATAATGTTTTTTCTCACGACCGGATTAGAATCACCGGCATCGCGAGGCCGCCCGCGCGGCGCATCGCATCC
GTTTCGCTTCCCTGCCATTC

Downstream 100 bases:

>100_bases
ACGCGTGATTGCCGCTTCATGGCCGATGGCAGACGATCGCCGCGTCCGGCGCGGCGATCGCGGCCGGTGACCGTTACTTC
GCCGGTACGGCCGGAATCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLTMQHLPDLEAWAIFARVAETGSFAKAADLLGISQPTVSKAIARLEKRLGAMLLYRTSRKLSLTPTGELLRDRAIRLLA
DAELIENEASAQALEPHGLVRVNAPMSFGLHHLSPVLPAFLERYPRVDIDLVLTDHIVDIVSGGFDVAIRIAELSDSTLR
SRRLCAVRRPLVASPAYLDRRGRPTHPRDLEQHVCLTYTNLPTPEYWRFRHPASGDEVGVSVHGRIRTNNADVILPALVG
GHGLALQPEFLVWDELQRGALEEVMSDWKIADINVNLVTPPGMLRAARVTVLLDFLAEHFSHAPWALPAA

Sequences:

>Translated_310_residues
MLTMQHLPDLEAWAIFARVAETGSFAKAADLLGISQPTVSKAIARLEKRLGAMLLYRTSRKLSLTPTGELLRDRAIRLLA
DAELIENEASAQALEPHGLVRVNAPMSFGLHHLSPVLPAFLERYPRVDIDLVLTDHIVDIVSGGFDVAIRIAELSDSTLR
SRRLCAVRRPLVASPAYLDRRGRPTHPRDLEQHVCLTYTNLPTPEYWRFRHPASGDEVGVSVHGRIRTNNADVILPALVG
GHGLALQPEFLVWDELQRGALEEVMSDWKIADINVNLVTPPGMLRAARVTVLLDFLAEHFSHAPWALPAA
>Mature_310_residues
MLTMQHLPDLEAWAIFARVAETGSFAKAADLLGISQPTVSKAIARLEKRLGAMLLYRTSRKLSLTPTGELLRDRAIRLLA
DAELIENEASAQALEPHGLVRVNAPMSFGLHHLSPVLPAFLERYPRVDIDLVLTDHIVDIVSGGFDVAIRIAELSDSTLR
SRRLCAVRRPLVASPAYLDRRGRPTHPRDLEQHVCLTYTNLPTPEYWRFRHPASGDEVGVSVHGRIRTNNADVILPALVG
GHGLALQPEFLVWDELQRGALEEVMSDWKIADINVNLVTPPGMLRAARVTVLLDFLAEHFSHAPWALPAA

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=35.2542372881356, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87081978, Length=303, Percent_Identity=30.6930693069307, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=29.2096219931272, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=30.2083333333333, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789639, Length=260, Percent_Identity=30.3846153846154, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789440, Length=284, Percent_Identity=26.7605633802817, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787589, Length=260, Percent_Identity=30.3846153846154, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789173, Length=259, Percent_Identity=29.3436293436293, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789506, Length=146, Percent_Identity=30.1369863013699, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790011, Length=260, Percent_Identity=25.7692307692308, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790399, Length=113, Percent_Identity=35.3982300884956, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788481, Length=275, Percent_Identity=25.4545454545455, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=144, Percent_Identity=30.5555555555556, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34327; Mature: 34327

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMQHLPDLEAWAIFARVAETGSFAKAADLLGISQPTVSKAIARLEKRLGAMLLYRTSR
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCC
KLSLTPTGELLRDRAIRLLADAELIENEASAQALEPHGLVRVNAPMSFGLHHLSPVLPAF
EEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCHHHCHHHHHHHHHHH
LERYPRVDIDLVLTDHIVDIVSGGFDVAIRIAELSDSTLRSRRLCAVRRPLVASPAYLDR
HHHCCCCCEEEEEHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHC
RGRPTHPRDLEQHVCLTYTNLPTPEYWRFRHPASGDEVGVSVHGRIRTNNADVILPALVG
CCCCCCCCCHHCCEEEEEECCCCCCCEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEEC
GHGLALQPEFLVWDELQRGALEEVMSDWKIADINVNLVTPPGMLRAARVTVLLDFLAEHF
CCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
SHAPWALPAA
CCCCCCCCCC
>Mature Secondary Structure
MLTMQHLPDLEAWAIFARVAETGSFAKAADLLGISQPTVSKAIARLEKRLGAMLLYRTSR
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCC
KLSLTPTGELLRDRAIRLLADAELIENEASAQALEPHGLVRVNAPMSFGLHHLSPVLPAF
EEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCHHHCHHHHHHHHHHH
LERYPRVDIDLVLTDHIVDIVSGGFDVAIRIAELSDSTLRSRRLCAVRRPLVASPAYLDR
HHHCCCCCEEEEEHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHC
RGRPTHPRDLEQHVCLTYTNLPTPEYWRFRHPASGDEVGVSVHGRIRTNNADVILPALVG
CCCCCCCCCHHCCEEEEEECCCCCCCEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEEC
GHGLALQPEFLVWDELQRGALEEVMSDWKIADINVNLVTPPGMLRAARVTVLLDFLAEHF
CCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
SHAPWALPAA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]