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The map label for this gene is acsA [H]

Identifier: 116689338

GI number: 116689338

Start: 1446920

End: 1448539

Strand: Reverse

Name: acsA [H]

Synonym: Bcen2424_1316

Alternate gene names: 116689338

Gene position: 1448539-1446920 (Counterclockwise)

Preceding gene: 116689339

Following gene: 116689337

Centisome position: 41.58

GC content: 70.31

Gene sequence:

>1620_bases
ATGCTGCCCGCCGCCGACCGCTACGATCACCTCCTCGCCCGCTTCCGCTGGGACATTCCGGCGCGCTACAACATCGGCGT
GGACGTGTGCGACAAATGGGCCGACGGCAGCGGACGCGTCGCGCTGATCCACGAAACGGCGCGAGGCAACGTTTCCCGCC
TCACGTTCGACGACCTGAAGCATGCTTCTAACCGGCTCGCGAACAGCTTCGCGCGTGCCGGCCTGCGGCGCGGCGACCGG
ATCGGCATCTTTCTCGCGCAAAGCCCCGAAACGGCCATCGCACACCTGGCCGCGTACAAGTTCGGCGCGATCGCGGTACC
GCTCTTCACGCTGTTCGGCGTCGATGCGCTCGAGTACCGGCTCGCGAACAGCGAGGCGGCCGCGCTCGTCACCGACGCGA
CCGGTTACGCGAAGATCGCGCCGCTGCGCCCGCAACTGCCGGCGCTGCATACCGTCTACTGCATCGGCGACGATGCGCCC
GACGTGCCCGGCGTGCTGCGCTACGACGCGGCGCTCGCCGCCGAGGCGCCGGATTTCGTGCCGGTGGATACGGCCGCCGA
CGATCCGGCGCTGATCATCTACACGTCCGGCACGACCGGCAAACCGAAGGGCGCGCTGCACGCGCATCGCGTGCTGCTCG
GCCACCTGCCGGGCGTCGAGATGTCGCAGCAGCGCTTCCCGCGCGACGCGCGCCTGTTCTGGACACCCGCCGACTGGGCA
TGGATCGGCGGGTTGCTCGACGTGCTGCTACCGTCATGGCATCACGGCGTGCCGGTGCTCGCGCACCGCTTCGAGAAATT
CGACGGCGACGCGGCGTTCGCGCTGATGGCGCGGCACGGCGTCACCCACGCCTTTCTGCCGCCCACCGCGCTGAAGCTGA
TGCGCGCCGTCGTGACGCCGCGCGAACGCTACACGCTGGCGCTGAAATCGGTCGCGAGCGGCGGCGAATCGCTCGGCACC
GAACTGACGGCGTGGGGCCGCGATGCGCTCGGCGTGACGATCAACGAGTTCTACGGGCAAACCGAATGCAACATGGTGCT
GTCGTCGTGTGCGGCGCTGTTCGATGCGCAGCCCGGCGTGATCGGCAAGGCGGTGCCCGGTCATGCGGTCGCGATCGTCG
ATGCGCACGGCACGCCGCTGCCGCCCGGCGTCGAAGGCCGCATCGCGGTGCGCCGCCCCGACCCGGTGATGTTTCTCGAA
TACTGGCGCAACCCCGACGCGACGCGCGACAAGTTCGCGGGCGACTACCTGCTCACCGGCGATACGGGCACGATCGACGC
CGACGGTTTCGTGCGCTTCGTCGGCCGCGACGACGACGTGATCACGAGCGCCGGTTACCGGATCGGCCCCGGCCCGATCG
AGGACTGCCTGCTCACGCATCCGGCGGTGCGGATGGCCGCCGTCGTCGGCGTGCCCGACGCGACGCGCACCGAGATCGTC
AAGGCGTTCGTCGTGCTGAACCCCGGCCACGTCGGCGACGCCGCGCTCGTCGGCGCGCTGCAGGCACATGTGCGCACGCG
GCTCGCCGCCCACGAGTACCCGCGCGCGATCGCGTTCGTCGACAGCCTGCCGATGACCGCGACCGGCAAGATCATCCGGC
GCGCGCTGCGCGACGCGTGA

Upstream 100 bases:

>100_bases
GAACATTCCCTATCAGCAAACACCATTGTGGATTCGTCTGCGTGAAATCCTGATGTAAGCCGTTCATCCGACGCCACGCT
CGACACCAGGAGACCTCGCG

Downstream 100 bases:

>100_bases
GCGCCCCGCTGCCGCTCGGCCCGCCCGGCCGGATGGTTTATAGTGGCGCCACGCAGTCTTTCCAAGAAACCGATGTCCTC
TGATCAACACGCCGCGGGCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acetyl-CoA synthase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLPAADRYDHLLARFRWDIPARYNIGVDVCDKWADGSGRVALIHETARGNVSRLTFDDLKHASNRLANSFARAGLRRGDR
IGIFLAQSPETAIAHLAAYKFGAIAVPLFTLFGVDALEYRLANSEAAALVTDATGYAKIAPLRPQLPALHTVYCIGDDAP
DVPGVLRYDAALAAEAPDFVPVDTAADDPALIIYTSGTTGKPKGALHAHRVLLGHLPGVEMSQQRFPRDARLFWTPADWA
WIGGLLDVLLPSWHHGVPVLAHRFEKFDGDAAFALMARHGVTHAFLPPTALKLMRAVVTPRERYTLALKSVASGGESLGT
ELTAWGRDALGVTINEFYGQTECNMVLSSCAALFDAQPGVIGKAVPGHAVAIVDAHGTPLPPGVEGRIAVRRPDPVMFLE
YWRNPDATRDKFAGDYLLTGDTGTIDADGFVRFVGRDDDVITSAGYRIGPGPIEDCLLTHPAVRMAAVVGVPDATRTEIV
KAFVVLNPGHVGDAALVGALQAHVRTRLAAHEYPRAIAFVDSLPMTATGKIIRRALRDA

Sequences:

>Translated_539_residues
MLPAADRYDHLLARFRWDIPARYNIGVDVCDKWADGSGRVALIHETARGNVSRLTFDDLKHASNRLANSFARAGLRRGDR
IGIFLAQSPETAIAHLAAYKFGAIAVPLFTLFGVDALEYRLANSEAAALVTDATGYAKIAPLRPQLPALHTVYCIGDDAP
DVPGVLRYDAALAAEAPDFVPVDTAADDPALIIYTSGTTGKPKGALHAHRVLLGHLPGVEMSQQRFPRDARLFWTPADWA
WIGGLLDVLLPSWHHGVPVLAHRFEKFDGDAAFALMARHGVTHAFLPPTALKLMRAVVTPRERYTLALKSVASGGESLGT
ELTAWGRDALGVTINEFYGQTECNMVLSSCAALFDAQPGVIGKAVPGHAVAIVDAHGTPLPPGVEGRIAVRRPDPVMFLE
YWRNPDATRDKFAGDYLLTGDTGTIDADGFVRFVGRDDDVITSAGYRIGPGPIEDCLLTHPAVRMAAVVGVPDATRTEIV
KAFVVLNPGHVGDAALVGALQAHVRTRLAAHEYPRAIAFVDSLPMTATGKIIRRALRDA
>Mature_539_residues
MLPAADRYDHLLARFRWDIPARYNIGVDVCDKWADGSGRVALIHETARGNVSRLTFDDLKHASNRLANSFARAGLRRGDR
IGIFLAQSPETAIAHLAAYKFGAIAVPLFTLFGVDALEYRLANSEAAALVTDATGYAKIAPLRPQLPALHTVYCIGDDAP
DVPGVLRYDAALAAEAPDFVPVDTAADDPALIIYTSGTTGKPKGALHAHRVLLGHLPGVEMSQQRFPRDARLFWTPADWA
WIGGLLDVLLPSWHHGVPVLAHRFEKFDGDAAFALMARHGVTHAFLPPTALKLMRAVVTPRERYTLALKSVASGGESLGT
ELTAWGRDALGVTINEFYGQTECNMVLSSCAALFDAQPGVIGKAVPGHAVAIVDAHGTPLPPGVEGRIAVRRPDPVMFLE
YWRNPDATRDKFAGDYLLTGDTGTIDADGFVRFVGRDDDVITSAGYRIGPGPIEDCLLTHPAVRMAAVVGVPDATRTEIV
KAFVVLNPGHVGDAALVGALQAHVRTRLAAHEYPRAIAFVDSLPMTATGKIIRRALRDA

Specific function: Has a role in growth and sporulation on acetate [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI115511026, Length=539, Percent_Identity=32.0964749536178, Blast_Score=275, Evalue=8e-74,
Organism=Homo sapiens, GI157311624, Length=543, Percent_Identity=33.1491712707182, Blast_Score=273, Evalue=4e-73,
Organism=Homo sapiens, GI157311622, Length=543, Percent_Identity=33.1491712707182, Blast_Score=273, Evalue=4e-73,
Organism=Homo sapiens, GI122937307, Length=543, Percent_Identity=33.5174953959484, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI42544132, Length=548, Percent_Identity=31.021897810219, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI38505220, Length=535, Percent_Identity=33.0841121495327, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI58082049, Length=543, Percent_Identity=32.780847145488, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI13375727, Length=563, Percent_Identity=29.1296625222025, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI28416953, Length=582, Percent_Identity=26.8041237113402, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI187761345, Length=546, Percent_Identity=28.021978021978, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI187761343, Length=546, Percent_Identity=28.021978021978, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI8923896, Length=579, Percent_Identity=27.2884283246978, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI253970489, Length=592, Percent_Identity=27.1959459459459, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI156151445, Length=523, Percent_Identity=26.3862332695985, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI195947378, Length=449, Percent_Identity=26.9487750556793, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI42544134, Length=381, Percent_Identity=24.1469816272966, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI40807357, Length=468, Percent_Identity=26.7094017094017, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788107, Length=575, Percent_Identity=27.6521739130435, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1790505, Length=560, Percent_Identity=26.7857142857143, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI221142682, Length=536, Percent_Identity=27.7985074626866, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786529, Length=582, Percent_Identity=26.8041237113402, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI145693145, Length=507, Percent_Identity=27.2189349112426, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1786810, Length=506, Percent_Identity=28.2608695652174, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1786801, Length=513, Percent_Identity=25.9259259259259, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788595, Length=431, Percent_Identity=26.9141531322506, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=553, Percent_Identity=26.7631103074141, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71982997, Length=553, Percent_Identity=26.7631103074141, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538037, Length=514, Percent_Identity=26.0700389105058, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=530, Percent_Identity=24.1509433962264, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=510, Percent_Identity=22.9411764705882, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17559526, Length=542, Percent_Identity=26.1992619926199, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560140, Length=518, Percent_Identity=24.9034749034749, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=24.0740740740741, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=22.7926078028747, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=521, Percent_Identity=24.5681381957774, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994694, Length=521, Percent_Identity=24.5681381957774, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=521, Percent_Identity=24.5681381957774, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=24.8587570621469, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=530, Percent_Identity=23.5849056603774, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=366, Percent_Identity=25.6830601092896, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=568, Percent_Identity=27.9929577464789, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319264, Length=567, Percent_Identity=28.042328042328, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6319699, Length=516, Percent_Identity=24.031007751938, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24667955, Length=603, Percent_Identity=27.1973466003317, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI62472339, Length=525, Percent_Identity=28, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21355225, Length=567, Percent_Identity=26.63139329806, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24667959, Length=489, Percent_Identity=27.6073619631902, Blast_Score=143, Evalue=4e-34,
Organism=Drosophila melanogaster, GI19922652, Length=504, Percent_Identity=26.984126984127, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24656500, Length=511, Percent_Identity=28.5714285714286, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI18859661, Length=531, Percent_Identity=25.2354048964218, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24581924, Length=527, Percent_Identity=25.9962049335863, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355181, Length=524, Percent_Identity=23.0916030534351, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356947, Length=488, Percent_Identity=23.3606557377049, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281365686, Length=512, Percent_Identity=22.265625, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648260, Length=486, Percent_Identity=22.8395061728395, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358303, Length=474, Percent_Identity=23.8396624472574, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI20130357, Length=494, Percent_Identity=23.6842105263158, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653035, Length=359, Percent_Identity=23.9554317548747, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356441, Length=506, Percent_Identity=23.3201581027668, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648253, Length=494, Percent_Identity=23.0769230769231, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648257, Length=422, Percent_Identity=25.1184834123223, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45550496, Length=556, Percent_Identity=22.1223021582734, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI45552801, Length=556, Percent_Identity=22.1223021582734, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648255, Length=494, Percent_Identity=22.8744939271255, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076582, Length=366, Percent_Identity=22.4043715846995, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 57976; Mature: 57976

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPAADRYDHLLARFRWDIPARYNIGVDVCDKWADGSGRVALIHETARGNVSRLTFDDLK
CCCCCHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCCEEEEEECCCCCEEEEEHHHHH
HASNRLANSFARAGLRRGDRIGIFLAQSPETAIAHLAAYKFGAIAVPLFTLFGVDALEYR
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHE
LANSEAAALVTDATGYAKIAPLRPQLPALHTVYCIGDDAPDVPGVLRYDAALAAEAPDFV
ECCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCE
PVDTAADDPALIIYTSGTTGKPKGALHAHRVLLGHLPGVEMSQQRFPRDARLFWTPADWA
ECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCHH
WIGGLLDVLLPSWHHGVPVLAHRFEKFDGDAAFALMARHGVTHAFLPPTALKLMRAVVTP
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHCC
RERYTLALKSVASGGESLGTELTAWGRDALGVTINEFYGQTECNMVLSSCAALFDAQPGV
CHHHHHHHHHHHCCCHHHCCCHHHCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHCCCCCC
IGKAVPGHAVAIVDAHGTPLPPGVEGRIAVRRPDPVMFLEYWRNPDATRDKFAGDYLLTG
CCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCHHHCCCCEEEEC
DTGTIDADGFVRFVGRDDDVITSAGYRIGPGPIEDCLLTHPAVRMAAVVGVPDATRTEIV
CCCCCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHCCCHHHHHHEECCCCCHHHHHE
KAFVVLNPGHVGDAALVGALQAHVRTRLAAHEYPRAIAFVDSLPMTATGKIIRRALRDA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLPAADRYDHLLARFRWDIPARYNIGVDVCDKWADGSGRVALIHETARGNVSRLTFDDLK
CCCCCHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCCCCEEEEEECCCCCEEEEEHHHHH
HASNRLANSFARAGLRRGDRIGIFLAQSPETAIAHLAAYKFGAIAVPLFTLFGVDALEYR
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHE
LANSEAAALVTDATGYAKIAPLRPQLPALHTVYCIGDDAPDVPGVLRYDAALAAEAPDFV
ECCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCE
PVDTAADDPALIIYTSGTTGKPKGALHAHRVLLGHLPGVEMSQQRFPRDARLFWTPADWA
ECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCCCHH
WIGGLLDVLLPSWHHGVPVLAHRFEKFDGDAAFALMARHGVTHAFLPPTALKLMRAVVTP
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHCC
RERYTLALKSVASGGESLGTELTAWGRDALGVTINEFYGQTECNMVLSSCAALFDAQPGV
CHHHHHHHHHHHCCCHHHCCCHHHCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHCCCCCC
IGKAVPGHAVAIVDAHGTPLPPGVEGRIAVRRPDPVMFLEYWRNPDATRDKFAGDYLLTG
CCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCHHHCCCCEEEEC
DTGTIDADGFVRFVGRDDDVITSAGYRIGPGPIEDCLLTHPAVRMAAVVGVPDATRTEIV
CCCCCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHCCCHHHHHHEECCCCCHHHHHE
KAFVVLNPGHVGDAALVGALQAHVRTRLAAHEYPRAIAFVDSLPMTATGKIIRRALRDA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934817; 9387221; 9384377 [H]