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The map label for this gene is nemA [H]

Identifier: 116689262

GI number: 116689262

Start: 1369929

End: 1371050

Strand: Reverse

Name: nemA [H]

Synonym: Bcen2424_1240

Alternate gene names: 116689262

Gene position: 1371050-1369929 (Counterclockwise)

Preceding gene: 116689273

Following gene: 116689261

Centisome position: 39.35

GC content: 67.91

Gene sequence:

>1122_bases
ATGACGATCGACAACTCTCCTTCCGGCCGGGCGGCATCGACCCGCGCCACGTTGTTCGACCCGCTCGAATTCGGCGGGAT
CACGCTCGGCAACCGCATCGTGATGGCACCGATGACGCGCAGCCGTGCCGGCGCCGGGGATGTACCCGGCGCGCTCAGCG
CCACCTACTACGCCCAACGCGCGTCGGCTGGCCTGATCGTCACGGAGGGCACGCAGATTTCGCCGCTCGGCAAGGGCTAT
CCGTTCACGCCGGGGATTTACAGCGATGCGCAAATCGACGGCTGGCAACGCGTGACCGACGCCGTGCATCGCGCAGGCGG
CCGGATTTTCGTCCAGCTCGCGCACGTCGGCCGCATCGGCCATCCCGATCTGCAGCCCGACGGCGCCCTGCCCGTCGCGC
CGTCGGCCATTCAGCCGGACGGACAGGCATTCACGCCGGCCGGACCGAAACCGCACCCCGTACCGCGCGCGCTCGAGACG
AATGAAATACCAGGCATCGTCGAGCAATACTCGCATGCGGCGCAGGCCGCGTTGCGGGCAGGTTTCGACGGCGTCGAAAT
CCACGCGGGGAACGGTTACCTGCTCGATCAGTTCCTGCGCGACGGTACGAACCGGCGCACGGACCCTTACGGCGGCACGA
TCGACAACCGTATCCGGCTGCCGCTCGACGTCGTGCGGGCGGTCGGACGTGTCGTGAGTGCCGCGCGCGTGGGCGTCCGC
ATTTCGCCGGTCACACCGCATTTCGGCGGCATCGCCGACAGTCAACCGCAGGCGCTGTTCGACCGGTTCGCCGACCGGCT
GACCGGGCTCGCCGCCTATCTTCACGTCGTCGAAGGCATTCCGCAGGTCGCGGCCGATCAGGCACCGCCGTTCGACTACC
GCGCATTGCGTCGTGCCTTCGGCGGGCTCTACATCGCGAACGGCGGCTACACGAAGGCGCGTGCGCAACAGGCACTCGAC
GACGGCCATGCCGACCTCGTGTCGTTCGCCTATCCGTTCATCGCGAACCCGGACTTCGTGACGCGTCTTCGACACGATCG
CCCGCTCGCGCACGCGGACATGTCCACCGCTTACGGCGGCGGTGAAGCCGGCTACACCGACTATCCGACGCTCGACGCGT
AA

Upstream 100 bases:

>100_bases
GCATTCTCCTAAATTCCTGTTCAGCAGCGCTCGACGCGTGCGCGCCGGGCATCCCGGATCGCGCGTCGACGCCCCATGAA
CACGACAGGCAAATGGAATC

Downstream 100 bases:

>100_bases
GCATCAATCGCCCGACAACAGGTCGGTCGCTCGACCGGATCGACGATCCACCCACTCATTCATTATGGATTCCATATGAT
CAATTCGATCAACCGCGCAC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTIDNSPSGRAASTRATLFDPLEFGGITLGNRIVMAPMTRSRAGAGDVPGALSATYYAQRASAGLIVTEGTQISPLGKGY
PFTPGIYSDAQIDGWQRVTDAVHRAGGRIFVQLAHVGRIGHPDLQPDGALPVAPSAIQPDGQAFTPAGPKPHPVPRALET
NEIPGIVEQYSHAAQAALRAGFDGVEIHAGNGYLLDQFLRDGTNRRTDPYGGTIDNRIRLPLDVVRAVGRVVSAARVGVR
ISPVTPHFGGIADSQPQALFDRFADRLTGLAAYLHVVEGIPQVAADQAPPFDYRALRRAFGGLYIANGGYTKARAQQALD
DGHADLVSFAYPFIANPDFVTRLRHDRPLAHADMSTAYGGGEAGYTDYPTLDA

Sequences:

>Translated_373_residues
MTIDNSPSGRAASTRATLFDPLEFGGITLGNRIVMAPMTRSRAGAGDVPGALSATYYAQRASAGLIVTEGTQISPLGKGY
PFTPGIYSDAQIDGWQRVTDAVHRAGGRIFVQLAHVGRIGHPDLQPDGALPVAPSAIQPDGQAFTPAGPKPHPVPRALET
NEIPGIVEQYSHAAQAALRAGFDGVEIHAGNGYLLDQFLRDGTNRRTDPYGGTIDNRIRLPLDVVRAVGRVVSAARVGVR
ISPVTPHFGGIADSQPQALFDRFADRLTGLAAYLHVVEGIPQVAADQAPPFDYRALRRAFGGLYIANGGYTKARAQQALD
DGHADLVSFAYPFIANPDFVTRLRHDRPLAHADMSTAYGGGEAGYTDYPTLDA
>Mature_372_residues
TIDNSPSGRAASTRATLFDPLEFGGITLGNRIVMAPMTRSRAGAGDVPGALSATYYAQRASAGLIVTEGTQISPLGKGYP
FTPGIYSDAQIDGWQRVTDAVHRAGGRIFVQLAHVGRIGHPDLQPDGALPVAPSAIQPDGQAFTPAGPKPHPVPRALETN
EIPGIVEQYSHAAQAALRAGFDGVEIHAGNGYLLDQFLRDGTNRRTDPYGGTIDNRIRLPLDVVRAVGRVVSAARVGVRI
SPVTPHFGGIADSQPQALFDRFADRLTGLAAYLHVVEGIPQVAADQAPPFDYRALRRAFGGLYIANGGYTKARAQQALDD
GHADLVSFAYPFIANPDFVTRLRHDRPLAHADMSTAYGGGEAGYTDYPTLDA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=44.4444444444444, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1789463, Length=362, Percent_Identity=31.4917127071823, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=252, Percent_Identity=28.968253968254, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=214, Percent_Identity=28.5046728971963, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=211, Percent_Identity=29.8578199052133, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=168, Percent_Identity=29.7619047619048, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=236, Percent_Identity=27.5423728813559, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=217, Percent_Identity=26.7281105990783, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=384, Percent_Identity=34.6354166666667, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321973, Length=384, Percent_Identity=34.375, Blast_Score=173, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39601; Mature: 39470

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDNSPSGRAASTRATLFDPLEFGGITLGNRIVMAPMTRSRAGAGDVPGALSATYYAQR
CCCCCCCCCCCCCCCHHHCCCHHHCCEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
ASAGLIVTEGTQISPLGKGYPFTPGIYSDAQIDGWQRVTDAVHRAGGRIFVQLAHVGRIG
CCCCEEEECCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEHHCCCC
HPDLQPDGALPVAPSAIQPDGQAFTPAGPKPHPVPRALETNEIPGIVEQYSHAAQAALRA
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GFDGVEIHAGNGYLLDQFLRDGTNRRTDPYGGTIDNRIRLPLDVVRAVGRVVSAARVGVR
CCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCEE
ISPVTPHFGGIADSQPQALFDRFADRLTGLAAYLHVVEGIPQVAADQAPPFDYRALRRAF
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHH
GGLYIANGGYTKARAQQALDDGHADLVSFAYPFIANPDFVTRLRHDRPLAHADMSTAYGG
CCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHCCCC
GEAGYTDYPTLDA
CCCCCCCCCCCCC
>Mature Secondary Structure 
TIDNSPSGRAASTRATLFDPLEFGGITLGNRIVMAPMTRSRAGAGDVPGALSATYYAQR
CCCCCCCCCCCCCCHHHCCCHHHCCEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
ASAGLIVTEGTQISPLGKGYPFTPGIYSDAQIDGWQRVTDAVHRAGGRIFVQLAHVGRIG
CCCCEEEECCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEHHCCCC
HPDLQPDGALPVAPSAIQPDGQAFTPAGPKPHPVPRALETNEIPGIVEQYSHAAQAALRA
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GFDGVEIHAGNGYLLDQFLRDGTNRRTDPYGGTIDNRIRLPLDVVRAVGRVVSAARVGVR
CCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCEE
ISPVTPHFGGIADSQPQALFDRFADRLTGLAAYLHVVEGIPQVAADQAPPFDYRALRRAF
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHH
GGLYIANGGYTKARAQQALDDGHADLVSFAYPFIANPDFVTRLRHDRPLAHADMSTAYGG
CCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHCCCC
GEAGYTDYPTLDA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]