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The map label for this gene is ywbI [H]

Identifier: 116689273

GI number: 116689273

Start: 1382368

End: 1383324

Strand: Reverse

Name: ywbI [H]

Synonym: Bcen2424_1251

Alternate gene names: 116689273

Gene position: 1383324-1382368 (Counterclockwise)

Preceding gene: 116689277

Following gene: 116689262

Centisome position: 39.71

GC content: 66.35

Gene sequence:

>957_bases
ATGCGCCCCGACCTCGCCCCGTTCCTGAACGACCGCCTCGACTGGAATCTGCTGCGCACCTACCTGGTGATCATGCAGGA
GCGCAGCGTGAGCCGAGCGGCCGCCCGCCTGCACGTCACGCAGCCGGCCGTGAGCCAGGCATTGCGGCGGCTCGAAGAGA
CGCTCGAGCGCCGGCTGATCGAGCGGCGCGGCGCGCACTTCGCGCCGACGCCGGCCGGCGAAGCCGTGTACCGGATCGCG
AGCGACATCTACGGCGGCATCTCGCGCCTCGAAACCGAAATCGACGACAGCACGGCCGACCTGACCGGCTCGATCCGGCT
GCTGACGGTGAGCCGCATCGAATCGCCCGTCTACGACGAATTCCTCGCGGATTTCCACCGCGCATATCCGCGTATCGATC
TGCAGATCGAAGTGATGCGCTCGTCGGACATCCTCTCCTCGCTGCTGCAGAAAACGGCCACGGCCGGGCTCGGCCTGTGC
CGCACGCCGCACGACAAGCTGGAAATGCGCTGCTTCCTGCGCCAACGCTATGCGATCTACTGCGGCCGCCATCACCGGTT
GTTCGGGCAAACGCAGTTGCGGATGGACGATCTGCTCGCGGAGAATTTCGTGTCGTTCACGAGCGACCAGATCGGCGACA
GCCTGTCGCCGCTCACCGTGTTCCGCGACCAGAAGGGCTTCACGGGACGCATCGTCGCGTCGTCGCCGAGCCTCGAGGAA
GTGCGGCGGCTGATCTTCGCGGGCTACGGGATCGGCTGCCTGCCCGAGCACATCGTGCGCGACGACATCGCGCAGCAACG
GCTGTGGCGGTTGCCGCCCGACGACGGGCTCGTCGATGTCGACGTGTTCCTGTTATGGCATCGCGACCGCAAGATGAACG
CCGCCGAGCTGGCGTTTCTCGACACGATGGAGCGCTGCATGCAGCGCTATTCGCTGGCGGAGCGGTTGGGGAAATGA

Upstream 100 bases:

>100_bases
CCCCTCCTTTATCGGTGGCCGCAAATTATTAGTCAAATTTCAAGTTATTATTCACGATATAAATTCAACTAATGCCTGAC
CGCTGCTGCCCGGACACCCG

Downstream 100 bases:

>100_bases
CAGCGCGCATGCGCTCGGAGCACGGCCGGGCGCATACGGCCCGGCGCGTCGCTCCGTGCGCATGCATCAGAAGCGATGCA
CGATGCCGAGTTGCACGCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRPDLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEETLERRLIERRGAHFAPTPAGEAVYRIA
SDIYGGISRLETEIDDSTADLTGSIRLLTVSRIESPVYDEFLADFHRAYPRIDLQIEVMRSSDILSSLLQKTATAGLGLC
RTPHDKLEMRCFLRQRYAIYCGRHHRLFGQTQLRMDDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDDGLVDVDVFLLWHRDRKMNAAELAFLDTMERCMQRYSLAERLGK

Sequences:

>Translated_318_residues
MRPDLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEETLERRLIERRGAHFAPTPAGEAVYRIA
SDIYGGISRLETEIDDSTADLTGSIRLLTVSRIESPVYDEFLADFHRAYPRIDLQIEVMRSSDILSSLLQKTATAGLGLC
RTPHDKLEMRCFLRQRYAIYCGRHHRLFGQTQLRMDDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDDGLVDVDVFLLWHRDRKMNAAELAFLDTMERCMQRYSLAERLGK
>Mature_318_residues
MRPDLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEETLERRLIERRGAHFAPTPAGEAVYRIA
SDIYGGISRLETEIDDSTADLTGSIRLLTVSRIESPVYDEFLADFHRAYPRIDLQIEVMRSSDILSSLLQKTATAGLGLC
RTPHDKLEMRCFLRQRYAIYCGRHHRLFGQTQLRMDDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDDGLVDVDVFLLWHRDRKMNAAELAFLDTMERCMQRYSLAERLGK

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787530, Length=259, Percent_Identity=24.7104247104247, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787589, Length=121, Percent_Identity=33.0578512396694, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=290, Percent_Identity=21.7241379310345, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=23.1833910034602, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=25.4295532646048, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36481; Mature: 36481

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPDLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEETLERRLI
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERRGAHFAPTPAGEAVYRIASDIYGGISRLETEIDDSTADLTGSIRLLTVSRIESPVYDE
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCHHHHH
FLADFHRAYPRIDLQIEVMRSSDILSSLLQKTATAGLGLCRTPHDKLEMRCFLRQRYAIY
HHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
CGRHHRLFGQTQLRMDDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
HCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCEEEEECCCCHHH
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDDGLVDVDVFLLWHRDRKMNAAELAFL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCHHHHHHH
DTMERCMQRYSLAERLGK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPDLAPFLNDRLDWNLLRTYLVIMQERSVSRAAARLHVTQPAVSQALRRLEETLERRLI
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERRGAHFAPTPAGEAVYRIASDIYGGISRLETEIDDSTADLTGSIRLLTVSRIESPVYDE
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHCCHHHHH
FLADFHRAYPRIDLQIEVMRSSDILSSLLQKTATAGLGLCRTPHDKLEMRCFLRQRYAIY
HHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
CGRHHRLFGQTQLRMDDLLAENFVSFTSDQIGDSLSPLTVFRDQKGFTGRIVASSPSLEE
HCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCEEEEECCCCHHH
VRRLIFAGYGIGCLPEHIVRDDIAQQRLWRLPPDDGLVDVDVFLLWHRDRKMNAAELAFL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCHHHHHHH
DTMERCMQRYSLAERLGK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]