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The map label for this gene is rfbE [H]

Identifier: 116622123

GI number: 116622123

Start: 3809690

End: 3811735

Strand: Direct

Name: rfbE [H]

Synonym: Acid_3011

Alternate gene names: 116622123

Gene position: 3809690-3811735 (Clockwise)

Preceding gene: 116622122

Following gene: 116622124

Centisome position: 38.23

GC content: 61.0

Gene sequence:

>2046_bases
ATGAAGCACCCATCCAAGAATGGGTCGTGCAAAACACGGAAGCCGCTGCTCGGGATCGTGGAATGGTTTCGCCCCGGAGA
ATACGAACGAGTGGAGGAGGTGCTCCGCGACTTGCGTGCGCTGGGCGTGCGTGAACTGCGGACCGGAATCCGCTGGTCGG
ACTGGTACGCGTCCGAAGGCGACGGCTGGTATGCGTGGCTGCTGCCACGCCTGGCGCAGGATGTCAACCTCCTGCCGTGC
TTCCTGTACACTCCCGCGCCCCTGGGAATCGTGCCGAAGTTCTCTTCCCCTCCGCGGACACCGAAGGCCTATGCGGATTT
CATCGACCTGATGATCACGCGATTCGGGCGTTACTTTGATTGGATCGAATTCTGGAATCAGCCAAACAACCTGACGGAAT
GGGACACCCGGATCGATCCCGATTGGCAGATGTTCTGCGAGATGATCGGTTGCGCGGCGTACTGGGCACGCAGCCGCGGG
AAGAAGACCGTTTTGCCGGGGACTTGGCCCATCGATTTGGACTGGCTGGAATTGATGCATGAGCGTGGTGTTCTGGCGTA
CATCGATGCGGTCGGCGTGCATGGGTTTCCCGGGGCGTCGGAGTTTGCGTGGCGCGGGTGGGAGACGGAAATCGGTGAGG
TACGGGCTCGGCTAGCCAGCTTCGGTGCGAAACCTCAGCTTTGGATCACGCAAACCGGCTATTCGACGTGGCGGGGCGAG
GAGCGCGCACAGGTGACCGCCTTCGCGGAAGCGGCGCATGCACCGGTGGAACGGGTCTACTGGCAGTCGGCGACGGATCG
CGATCCCTCGCTGTCGAACGGGGAATCGTTCCAGAGCGACGAGAGGCAGTACCACCTCGGATTGAAGCGGGTGGATGGGA
CACCGAAGCTGCTGTTCCAACTGTGGTCTGAGGGCGGCCTTGAAGCCGTGCGGGAAGGTGTGCGATCCACCGCTCGCTCG
GCAGCTCGACGTCGCGGACACACTCTAATCACGGGAGGCGCGGGATTCATCGGGACAAATTTGGCGGACCGGCTGTTGAC
GGCAGGACGGAACGTGCTGATCTATGACGATCTCTCGCGGTCCGGGTCTGAACGGAATGTCGCGTGGCTGCGCGAGTCGC
ACGGAGAACGGCTAAACGTGGAGATCGCCGATATTCGGAATCGCCAGTCGCTCCGGACGGCGGTTCGCGAGGCCGACCAG
GTCTTTCACTTTGCGGCGCAGGTGGCGGTGACGTCCAGTCTGGTGGATCCGCTGCACGATTTCGAAGTCAATGTGGGCGG
CACCGTGAACCTGCTGGAAGAGATCCGCCGGTTGGACAATCCGCCGCCCCTGCTTTTCACCTCGACGAACAAGGTGTACG
GCGGACTTCCGGACCTGGCGCTGGAAAAGAGTTCGGCGCGTTATCAGCCGCTCGACGCGGCGTTGCGCACGGGAATCAGC
GAGGAGCGGCCGCTTGACTTCCACAGTCCCTATGGCTGCTCGAAAGGCGCGGCCGATCAATACGTCCGCGACTATGCCCG
GACATTCGGATTGCCGGCAGTGGTGTTCCGCATGAGTTGTATTTATGGGCTGCACCAGATGGGCAACGAAGATCAGGGTT
GGGTGGCGCACTTTCTCATTCGCGCGCTCGAAGGCAAACCGATCACGCTATTCGGCGACGGCATGCAGGTACGCGACATC
TTATTCGTAGATGATCTGGTGGACGCATTCCTGCTGGCGCAGGCAAATATTCACACGCTTGCCGGGCAGGCCTTCAACAT
CGGCGGCGGATTGGGAAATACGATCAGCTTGCTGGAACTGCTCGAATTGATTGAGCGTTTCCGAGACGAGAAGCCGATCG
TCCGACTGAAGCCCGCTCGTCCCAGCGACCAACGCTATTACGTCTCCGACACACGTAAATTCAAGGCGGCCACGGGATGG
GCGCCCAAAGTGACCGCGCGTGAAGGCGTGGAGCGCCTATTTCAATGGCTCGCGGAATCGCGCGGCCTTCCAGTGCCGAA
TGAGCTACTTGGGAAGGGGGCTTTTCATGCGCTTCTCGCTTGTTAA

Upstream 100 bases:

>100_bases
AGCAGGGGTTGGTGAACCTGGCGGAATGGCTGGAGAGCCAGACTGCGGTGGATCGCGGTATGGAGGCACGGGCGGAACTG
GCTGCCCGTGGGCTGATGGT

Downstream 100 bases:

>100_bases
CCCGCCGTGGAGTTTCACGGGCAGCATTTATTTCGGTTGCCGCGAACCGCACCTGCCGCTTGAGTACGGGTATGCGCGGA
ACCTGCTGGAGAATGCCGGG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MKHPSKNGSCKTRKPLLGIVEWFRPGEYERVEEVLRDLRALGVRELRTGIRWSDWYASEGDGWYAWLLPRLAQDVNLLPC
FLYTPAPLGIVPKFSSPPRTPKAYADFIDLMITRFGRYFDWIEFWNQPNNLTEWDTRIDPDWQMFCEMIGCAAYWARSRG
KKTVLPGTWPIDLDWLELMHERGVLAYIDAVGVHGFPGASEFAWRGWETEIGEVRARLASFGAKPQLWITQTGYSTWRGE
ERAQVTAFAEAAHAPVERVYWQSATDRDPSLSNGESFQSDERQYHLGLKRVDGTPKLLFQLWSEGGLEAVREGVRSTARS
AARRRGHTLITGGAGFIGTNLADRLLTAGRNVLIYDDLSRSGSERNVAWLRESHGERLNVEIADIRNRQSLRTAVREADQ
VFHFAAQVAVTSSLVDPLHDFEVNVGGTVNLLEEIRRLDNPPPLLFTSTNKVYGGLPDLALEKSSARYQPLDAALRTGIS
EERPLDFHSPYGCSKGAADQYVRDYARTFGLPAVVFRMSCIYGLHQMGNEDQGWVAHFLIRALEGKPITLFGDGMQVRDI
LFVDDLVDAFLLAQANIHTLAGQAFNIGGGLGNTISLLELLELIERFRDEKPIVRLKPARPSDQRYYVSDTRKFKAATGW
APKVTAREGVERLFQWLAESRGLPVPNELLGKGAFHALLAC

Sequences:

>Translated_681_residues
MKHPSKNGSCKTRKPLLGIVEWFRPGEYERVEEVLRDLRALGVRELRTGIRWSDWYASEGDGWYAWLLPRLAQDVNLLPC
FLYTPAPLGIVPKFSSPPRTPKAYADFIDLMITRFGRYFDWIEFWNQPNNLTEWDTRIDPDWQMFCEMIGCAAYWARSRG
KKTVLPGTWPIDLDWLELMHERGVLAYIDAVGVHGFPGASEFAWRGWETEIGEVRARLASFGAKPQLWITQTGYSTWRGE
ERAQVTAFAEAAHAPVERVYWQSATDRDPSLSNGESFQSDERQYHLGLKRVDGTPKLLFQLWSEGGLEAVREGVRSTARS
AARRRGHTLITGGAGFIGTNLADRLLTAGRNVLIYDDLSRSGSERNVAWLRESHGERLNVEIADIRNRQSLRTAVREADQ
VFHFAAQVAVTSSLVDPLHDFEVNVGGTVNLLEEIRRLDNPPPLLFTSTNKVYGGLPDLALEKSSARYQPLDAALRTGIS
EERPLDFHSPYGCSKGAADQYVRDYARTFGLPAVVFRMSCIYGLHQMGNEDQGWVAHFLIRALEGKPITLFGDGMQVRDI
LFVDDLVDAFLLAQANIHTLAGQAFNIGGGLGNTISLLELLELIERFRDEKPIVRLKPARPSDQRYYVSDTRKFKAATGW
APKVTAREGVERLFQWLAESRGLPVPNELLGKGAFHALLAC
>Mature_681_residues
MKHPSKNGSCKTRKPLLGIVEWFRPGEYERVEEVLRDLRALGVRELRTGIRWSDWYASEGDGWYAWLLPRLAQDVNLLPC
FLYTPAPLGIVPKFSSPPRTPKAYADFIDLMITRFGRYFDWIEFWNQPNNLTEWDTRIDPDWQMFCEMIGCAAYWARSRG
KKTVLPGTWPIDLDWLELMHERGVLAYIDAVGVHGFPGASEFAWRGWETEIGEVRARLASFGAKPQLWITQTGYSTWRGE
ERAQVTAFAEAAHAPVERVYWQSATDRDPSLSNGESFQSDERQYHLGLKRVDGTPKLLFQLWSEGGLEAVREGVRSTARS
AARRRGHTLITGGAGFIGTNLADRLLTAGRNVLIYDDLSRSGSERNVAWLRESHGERLNVEIADIRNRQSLRTAVREADQ
VFHFAAQVAVTSSLVDPLHDFEVNVGGTVNLLEEIRRLDNPPPLLFTSTNKVYGGLPDLALEKSSARYQPLDAALRTGIS
EERPLDFHSPYGCSKGAADQYVRDYARTFGLPAVVFRMSCIYGLHQMGNEDQGWVAHFLIRALEGKPITLFGDGMQVRDI
LFVDDLVDAFLLAQANIHTLAGQAFNIGGGLGNTISLLELLELIERFRDEKPIVRLKPARPSDQRYYVSDTRKFKAATGW
APKVTAREGVERLFQWLAESRGLPVPNELLGKGAFHALLAC

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=28.1524926686217, Blast_Score=120, Evalue=6e-27,
Organism=Homo sapiens, GI42516563, Length=400, Percent_Identity=25, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI56237023, Length=354, Percent_Identity=27.1186440677966, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI56118217, Length=354, Percent_Identity=27.1186440677966, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI189083684, Length=354, Percent_Identity=27.1186440677966, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788353, Length=345, Percent_Identity=30.4347826086957, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=29.7994269340974, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1786974, Length=352, Percent_Identity=28.125, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=346, Percent_Identity=28.9017341040462, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17539532, Length=354, Percent_Identity=26.271186440678, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=27.810650887574, Blast_Score=93, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=357, Percent_Identity=24.9299719887955, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=359, Percent_Identity=24.7910863509749, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=24.5810055865922, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356223, Length=364, Percent_Identity=26.3736263736264, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19923002, Length=358, Percent_Identity=25.6983240223464, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 76786; Mature: 76786

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHPSKNGSCKTRKPLLGIVEWFRPGEYERVEEVLRDLRALGVRELRTGIRWSDWYASEG
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCC
DGWYAWLLPRLAQDVNLLPCFLYTPAPLGIVPKFSSPPRTPKAYADFIDLMITRFGRYFD
CCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
WIEFWNQPNNLTEWDTRIDPDWQMFCEMIGCAAYWARSRGKKTVLPGTWPIDLDWLELMH
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHH
ERGVLAYIDAVGVHGFPGASEFAWRGWETEIGEVRARLASFGAKPQLWITQTGYSTWRGE
HCCCEEEEEHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
ERAQVTAFAEAAHAPVERVYWQSATDRDPSLSNGESFQSDERQYHLGLKRVDGTPKLLFQ
CHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCHHHCCCCHHHHHH
LWSEGGLEAVREGVRSTARSAARRRGHTLITGGAGFIGTNLADRLLTAGRNVLIYDDLSR
HHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCC
SGSERNVAWLRESHGERLNVEIADIRNRQSLRTAVREADQVFHFAAQVAVTSSLVDPLHD
CCCCCCHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEVNVGGTVNLLEEIRRLDNPPPLLFTSTNKVYGGLPDLALEKSSARYQPLDAALRTGIS
CEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHCCCC
EERPLDFHSPYGCSKGAADQYVRDYARTFGLPAVVFRMSCIYGLHQMGNEDQGWVAHFLI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RALEGKPITLFGDGMQVRDILFVDDLVDAFLLAQANIHTLAGQAFNIGGGLGNTISLLEL
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCCCCCHHHHHHHH
LELIERFRDEKPIVRLKPARPSDQRYYVSDTRKFKAATGWAPKVTAREGVERLFQWLAES
HHHHHHHCCCCCEEEEECCCCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHC
RGLPVPNELLGKGAFHALLAC
CCCCCCHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKHPSKNGSCKTRKPLLGIVEWFRPGEYERVEEVLRDLRALGVRELRTGIRWSDWYASEG
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCC
DGWYAWLLPRLAQDVNLLPCFLYTPAPLGIVPKFSSPPRTPKAYADFIDLMITRFGRYFD
CCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
WIEFWNQPNNLTEWDTRIDPDWQMFCEMIGCAAYWARSRGKKTVLPGTWPIDLDWLELMH
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHH
ERGVLAYIDAVGVHGFPGASEFAWRGWETEIGEVRARLASFGAKPQLWITQTGYSTWRGE
HCCCEEEEEHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
ERAQVTAFAEAAHAPVERVYWQSATDRDPSLSNGESFQSDERQYHLGLKRVDGTPKLLFQ
CHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCHHHCCCCHHHHHH
LWSEGGLEAVREGVRSTARSAARRRGHTLITGGAGFIGTNLADRLLTAGRNVLIYDDLSR
HHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCC
SGSERNVAWLRESHGERLNVEIADIRNRQSLRTAVREADQVFHFAAQVAVTSSLVDPLHD
CCCCCCHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEVNVGGTVNLLEEIRRLDNPPPLLFTSTNKVYGGLPDLALEKSSARYQPLDAALRTGIS
CEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHCCCC
EERPLDFHSPYGCSKGAADQYVRDYARTFGLPAVVFRMSCIYGLHQMGNEDQGWVAHFLI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RALEGKPITLFGDGMQVRDILFVDDLVDAFLLAQANIHTLAGQAFNIGGGLGNTISLLEL
HHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCCCCCHHHHHHHH
LELIERFRDEKPIVRLKPARPSDQRYYVSDTRKFKAATGWAPKVTAREGVERLFQWLAES
HHHHHHHCCCCCEEEEECCCCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHC
RGLPVPNELLGKGAFHALLAC
CCCCCCHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]