Click here to switch to the map view.

The map label for this gene is bchE [H]

Identifier: 116622124

GI number: 116622124

Start: 3811716

End: 3813005

Strand: Direct

Name: bchE [H]

Synonym: Acid_3012

Alternate gene names: 116622124

Gene position: 3811716-3813005 (Clockwise)

Preceding gene: 116622123

Following gene: 116622125

Centisome position: 38.25

GC content: 61.94

Gene sequence:

>1290_bases
ATGCGCTTCTCGCTTGTTAACCCGCCGTGGAGTTTCACGGGCAGCATTTATTTCGGTTGCCGCGAACCGCACCTGCCGCT
TGAGTACGGGTATGCGCGGAACCTGCTGGAGAATGCCGGGCACACGGTGCAGTTGGTGGACGGGCAACTGGAGAGCCTCA
GCTTGGATGAGATGCGCCGGCGCGTGGCGGACATCCGTCCCGATTTCACGGTCGTCACAACGGCCCCGAGTTACCTCTTT
TGGCGATGCGCGCCGCCGGAACTGCGGGTGCCGCGCGAAACGATAGATGCGCTCCGCGACGTCGGCGGTACGATGATCGG
CGTAGGTCCGCACGCCTCGACCACGCCGAAGACGGCGCTGCGGAAGCTCGGCGCGGATGCGGTGGTGCTCGGCGAATGCG
AAGAGGTTCTGCCGAGACTGGTCGGCGATTGGCGCTACGTGGACTCGCTCTGTTACCGCCGCGGGGACGCAGTGAGCGTG
AACGGCGGCCCTCACGCGTCGGATATGACGACTCTTTCCGCGCTTCACTGGAATAGCGAACTGATCGGTCGGCACGCACA
CCATCATCATCGGTTCGATTCGCCGCAGTACGGTGCGGGCGCGGAAATGGAGACATCGCGCGGCTGCCCGTATCACTGTA
CTTTCTGCGCGAAAGACAATTTCCGAAACCGCTTCCGTAGGCGGCCGCTGGCGACCATCGCCGAGGAACTCGATGGCCTG
ATTGCGGCCGGCGCGGGTTACGTGTACTTCATCGACGAGATCTTTCTTCCGTATCGGGATGTGCTGGAAACGATTCGGGA
GCGCAAGATTGTTTTCGGCATTCAAACTCGTATCGATTTGTGGAACGAGGAAATGATCGATCTGCTTGGCGATGCGGGAT
GCGTGTCGATTGAAGCAGGAGTGGAGAGCATTACCGAGGCGGGGCGGGAGTTGCTCGATAAGAAGTGCAAGATCGGCACC
GAGGAGATCGGCGAGCGCCTCATCCATGCCAAGCGGCGCGTATCTTTCGTGCAGGCTAATCTGCTGGATTCCCGTGACGA
CGACCCGGCCGACGTGCAGAAATGGAGGCAGCATCTTCAGGCGAATGGCGTGTGGGCAAACGAACCGGTGCCGCTCTTCC
CGTATCCCGGCTCGCCCGATTACACCCGGCGCTGGGGAGCGCCTGACGACTTGGCCTGGGAGCGTGCGCATAGGTCGTAC
CTGGAGGCATTCGACACGTTCAGCGATATCCAGGAGCAGCGACCGGAGCCGCTGGTGCAACTCGAAATCCCGGAGGGCCC
GCATGCCTGA

Upstream 100 bases:

>100_bases
AAAGTGACCGCGCGTGAAGGCGTGGAGCGCCTATTTCAATGGCTCGCGGAATCGCGCGGCCTTCCAGTGCCGAATGAGCT
ACTTGGGAAGGGGGCTTTTC

Downstream 100 bases:

>100_bases
CGCCGACGTGCGGCGGGTGCTGATGACAGGCGACACGGTGGGCGGAGTTTGGACCTTTACGCTGGATCTGGCGAGAGCAC
TGCAGCCGTGGAATATCGAA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: Anaerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRFSLVNPPWSFTGSIYFGCREPHLPLEYGYARNLLENAGHTVQLVDGQLESLSLDEMRRRVADIRPDFTVVTTAPSYLF
WRCAPPELRVPRETIDALRDVGGTMIGVGPHASTTPKTALRKLGADAVVLGECEEVLPRLVGDWRYVDSLCYRRGDAVSV
NGGPHASDMTTLSALHWNSELIGRHAHHHHRFDSPQYGAGAEMETSRGCPYHCTFCAKDNFRNRFRRRPLATIAEELDGL
IAAGAGYVYFIDEIFLPYRDVLETIRERKIVFGIQTRIDLWNEEMIDLLGDAGCVSIEAGVESITEAGRELLDKKCKIGT
EEIGERLIHAKRRVSFVQANLLDSRDDDPADVQKWRQHLQANGVWANEPVPLFPYPGSPDYTRRWGAPDDLAWERAHRSY
LEAFDTFSDIQEQRPEPLVQLEIPEGPHA

Sequences:

>Translated_429_residues
MRFSLVNPPWSFTGSIYFGCREPHLPLEYGYARNLLENAGHTVQLVDGQLESLSLDEMRRRVADIRPDFTVVTTAPSYLF
WRCAPPELRVPRETIDALRDVGGTMIGVGPHASTTPKTALRKLGADAVVLGECEEVLPRLVGDWRYVDSLCYRRGDAVSV
NGGPHASDMTTLSALHWNSELIGRHAHHHHRFDSPQYGAGAEMETSRGCPYHCTFCAKDNFRNRFRRRPLATIAEELDGL
IAAGAGYVYFIDEIFLPYRDVLETIRERKIVFGIQTRIDLWNEEMIDLLGDAGCVSIEAGVESITEAGRELLDKKCKIGT
EEIGERLIHAKRRVSFVQANLLDSRDDDPADVQKWRQHLQANGVWANEPVPLFPYPGSPDYTRRWGAPDDLAWERAHRSY
LEAFDTFSDIQEQRPEPLVQLEIPEGPHA
>Mature_429_residues
MRFSLVNPPWSFTGSIYFGCREPHLPLEYGYARNLLENAGHTVQLVDGQLESLSLDEMRRRVADIRPDFTVVTTAPSYLF
WRCAPPELRVPRETIDALRDVGGTMIGVGPHASTTPKTALRKLGADAVVLGECEEVLPRLVGDWRYVDSLCYRRGDAVSV
NGGPHASDMTTLSALHWNSELIGRHAHHHHRFDSPQYGAGAEMETSRGCPYHCTFCAKDNFRNRFRRRPLATIAEELDGL
IAAGAGYVYFIDEIFLPYRDVLETIRERKIVFGIQTRIDLWNEEMIDLLGDAGCVSIEAGVESITEAGRELLDKKCKIGT
EEIGERLIHAKRRVSFVQANLLDSRDDDPADVQKWRQHLQANGVWANEPVPLFPYPGSPDYTRRWGAPDDLAWERAHRSY
LEAFDTFSDIQEQRPEPLVQLEIPEGPHA

Specific function: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis in anaerobic conditions. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-prot

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011772
- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 48479; Mature: 48479

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSLVNPPWSFTGSIYFGCREPHLPLEYGYARNLLENAGHTVQLVDGQLESLSLDEMRR
CCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
RVADIRPDFTVVTTAPSYLFWRCAPPELRVPRETIDALRDVGGTMIGVGPHASTTPKTAL
HHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHH
RKLGADAVVLGECEEVLPRLVGDWRYVDSLCYRRGDAVSVNGGPHASDMTTLSALHWNSE
HHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCHH
LIGRHAHHHHRFDSPQYGAGAEMETSRGCPYHCTFCAKDNFRNRFRRRPLATIAEELDGL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCHHHHHHHHHHHH
IAAGAGYVYFIDEIFLPYRDVLETIRERKIVFGIQTRIDLWNEEMIDLLGDAGCVSIEAG
HHCCCCCEEEHHHHHCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCEEEHHHH
VESITEAGRELLDKKCKIGTEEIGERLIHAKRRVSFVQANLLDSRDDDPADVQKWRQHLQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ANGVWANEPVPLFPYPGSPDYTRRWGAPDDLAWERAHRSYLEAFDTFSDIQEQRPEPLVQ
CCCEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LEIPEGPHA
EECCCCCCC
>Mature Secondary Structure
MRFSLVNPPWSFTGSIYFGCREPHLPLEYGYARNLLENAGHTVQLVDGQLESLSLDEMRR
CCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
RVADIRPDFTVVTTAPSYLFWRCAPPELRVPRETIDALRDVGGTMIGVGPHASTTPKTAL
HHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHH
RKLGADAVVLGECEEVLPRLVGDWRYVDSLCYRRGDAVSVNGGPHASDMTTLSALHWNSE
HHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCHH
LIGRHAHHHHRFDSPQYGAGAEMETSRGCPYHCTFCAKDNFRNRFRRRPLATIAEELDGL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCHHHHHHHHHHHH
IAAGAGYVYFIDEIFLPYRDVLETIRERKIVFGIQTRIDLWNEEMIDLLGDAGCVSIEAG
HHCCCCCEEEHHHHHCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCEEEHHHH
VESITEAGRELLDKKCKIGTEEIGERLIHAKRRVSFVQANLLDSRDDDPADVQKWRQHLQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ANGVWANEPVPLFPYPGSPDYTRRWGAPDDLAWERAHRSYLEAFDTFSDIQEQRPEPLVQ
CCCEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LEIPEGPHA
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8385667; 2203738; 10841582 [H]