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The map label for this gene is leuS [H]

Identifier: 116517205

GI number: 116517205

Start: 227270

End: 229771

Strand: Direct

Name: leuS [H]

Synonym: SPD_0238

Alternate gene names: 116517205

Gene position: 227270-229771 (Clockwise)

Preceding gene: 116515896

Following gene: 116515892

Centisome position: 11.11

GC content: 44.6

Gene sequence:

>2502_bases
ATGAGTTTTTATAATCATAAAGAAATTGAGCCTAAGTGGCAGGGCTACTGGGCAGAACATCATACATTTAAGACAGGAAC
AGATGCATCAAAACCTAAGTTTTATGCGCTTGATATGTTCCCTTATCCGTCTGGAGTTGGTCTGCACGTAGGACACCCAG
AAGGTTATACTGCAACCGATATCCTCAGTCGTTACAAACGTGCGCAAGGCTACAATGTCCTTCACCCAATGGGTTGGGAT
GCTTTTGGTTTGCCTGCAGAGCAATACGCTATGGATACTGGTAATGACCCAGCAGAATTTACAGCGGAAAACATTGCCAA
CTTCAAACGTCAAATCAATGCGCTTGGATTTTCTTATGACTGGGATCGTGAAGTCAACACAACAGATCCAAACTACTACA
AGTGGACTCAATGGATTTTCACCAAGCTTTACGAAAAAGGCTTGGCCTATGAAGCTGAAGTGCCAGTAAACTGGGTTGAG
GAATTGGGAACTGCCATTGCCAATGAAGAAGTGCTTCCTGACGGAACTTCTGAGCGTGGAGGCTATCCAGTTGTCCGCAA
ACCAATGCGCCAATGGATGCTCAAAATCACGGCTTACGCAGAGCGCTTGCTCAATGACTTAGATGAACTAGATTGGTCAG
AGTCTATCAAGGATATGCAACGCAACTGGATTGGTAAATCAACTGGTGCCAATGTAACTTTCAAAGTAAAAGGAACAGAC
AAGGAATTTACAGTCTTTACTACTCGTCCGGACACACTTTTCGGTGCGACTTTCACTGTCTTGGCTCCTGAACATGAATT
AGTAGACGCTATCACAAGTTCAGAGCAAGCAGAAGCTGTAGCAGACTATAAACACCAAGCCAGCCTTAAGTCTGACTTGG
TTCGTACAGACCTTGCTAAGGAAAAAACTGGTGTTTGGACAGGTGCCTATGCCATCAACCCTGTCAATGGTAAGGAAATG
CCAATCTGGATTGCGGACTATGTTCTTGCTAGTTATGGAACAGGTGCGGTTATGGCTGTGCCTGCCCACGACCAACGTGA
CTGGGAATTTGCCAAACAATTTGACCTTCCAATCGTCGAAGTACTTGAAGGTGGAAATGTCGAAGAAGCTGCCTACACAG
AGGATGGCCTGCATGTCAATTCAGACTTCCTAGATGGATTGAACAAAGAAGACGCTATTGCCAAGATTGTGGCTTGCTTG
GAAGAAAAAGGCTGTGGTCAGGAGAAGGTTACCTACCGCCTCCGCGACTGGCTCTTTAGCCGTCAACGTTACTGGGGTGA
GCCAATTCCAATCATTCATTGGGAAGATGGAACTTCAACAGCTGTTCCTGAAACTGAATTGCCGCTTGTCTTGCCTGTAA
CCAAGGATATCCGTCCTTCAGGTACTGGTGAAAGTCCACTAGCTAACTTGACAGATTGGCTTGAAGTGACTCGTGAAGAT
GGTGTCAAAGGTCGTCGTGAAACTAACACCATGCCACAATGGGCTGGTTCAAGCTGGTACTACCTCCGCTATATTGACCC
GCACAATACTGAGAAATTGGCTGATGAGGACCTCCTCAAACAATGGTTGCCAGTAGATATCTACGTGGGTGGTGCGGAAC
ATGCTGTACTTCACTTGCTTTATGCTCGTTTCTGGCATAAATTCCTCTATGACCTCGGTGTTGTTCCGACTAAGGAACCA
TTCCAAAAACTCTTTAACCAAGGGATGATTTTGGGAACAAGCTACCGTGACCACCGTGGTGCTCTTGTGGCAACCGACAA
GGTTGAAAAACGTGATGGTTCCTTCTTTCATATAGAAACAGGGGAAGAGTTGGAGCAAGCGCCAGCCAAGATGTCTAAAT
CGCTCAAGAACGTTGTTAACCCAGATGATGTGGTGGAACAATACGGTGCCGATACCCTTCGTGTTTATGAAATGTTTATG
GGACCACTCGATGCTTCGATTGCTTGGTCAGAAGAAGGTTTGGAAGGAAGCCGTAAGTTCCTTGACCGAGTTTACCGTTT
GATTACAAGTAAAGAAATCCTTGCGGAAAACAATGGTGCTCTTGACAAGGCTTACAACGAAACAGTCAAAGCTGTCACAG
AACAAATTGAGTCTCTCAAATTCAACACAGCTATTGCCCAACTTATGGTCTTTGTCAATGCTGCTAACAAGGAAGATAAG
CTTTATGTTGACTATGCCAAAGGCTTTATTCAATTGATTGCACCATTTGCACCTCACTTGGCAGAAGAACTCTGGCAAAC
AGTCGCAGAAACAGGTGAGTCAATCTCTTATGTAGCTTGGCCAACTTGGGACGAAAGTAAACTGGTTGAAGACGAAATCG
AAATCGTTGTCCAAATCAAAGGTAAAGTCCGTGCCAAACTCATGGTCGCTAAAGACCTATCACGTGAAGAATTGCAAGAA
ATTGCTCTAGCGGATGAAAAAGTCAAAGCAGAAATTGACGGTAAGGAAATCGTGAAAGTGATTAGTGTACCAAATAAATT
GGTTAATATTGTTGTAAAATAA

Upstream 100 bases:

>100_bases
TCAAAATTAATGAGATACAGTATACAGTTTTTTCTTTAAACACATTTCAAATTCCCTCAAAAATGGTATAATAGTAACAT
CACAAAATTGGAGAGAGACC

Downstream 100 bases:

>100_bases
CCAATTTATTTGCTGATTTCCAACCTCAAACAGTTTCCCAAACTGTTTGAGCCAACTAAACTCGTTAATATCGTTGTGAA
ATAAGGTAGGAATCCTTCAG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGVGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKDMQRNWIGKSTGANVTFKVKGTD
KEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQAEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEM
PIWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVACL
EEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEP
FQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALDKAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDK
LYVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREELQE
IALADEKVKAEIDGKEIVKVISVPNKLVNIVVK

Sequences:

>Translated_833_residues
MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGVGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKDMQRNWIGKSTGANVTFKVKGTD
KEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQAEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEM
PIWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVACL
EEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEP
FQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALDKAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDK
LYVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREELQE
IALADEKVKAEIDGKEIVKVISVPNKLVNIVVK
>Mature_832_residues
SFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGVGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDA
FGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEE
LGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKDMQRNWIGKSTGANVTFKVKGTDK
EFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQAEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMP
IWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVACLE
EKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLVLPVTKDIRPSGTGESPLANLTDWLEVTREDG
VKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPF
QKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMG
PLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALDKAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKL
YVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREELQEI
ALADEKVKAEIDGKEIVKVISVPNKLVNIVVK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=881, Percent_Identity=33.5981838819523, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI94721239, Length=181, Percent_Identity=28.7292817679558, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI94721241, Length=181, Percent_Identity=28.7292817679558, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1786861, Length=865, Percent_Identity=42.7745664739884, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=398, Percent_Identity=26.8844221105528, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71997517, Length=775, Percent_Identity=30.9677419354839, Blast_Score=353, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI71997510, Length=775, Percent_Identity=30.9677419354839, Blast_Score=352, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI212645227, Length=372, Percent_Identity=30.3763440860215, Blast_Score=162, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71980946, Length=150, Percent_Identity=32.6666666666667, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=860, Percent_Identity=35.4651162790698, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321531, Length=433, Percent_Identity=26.5588914549654, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319395, Length=180, Percent_Identity=28.3333333333333, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21355409, Length=750, Percent_Identity=34.6666666666667, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17864482, Length=456, Percent_Identity=23.9035087719298, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24653289, Length=456, Percent_Identity=23.9035087719298, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI281366294, Length=375, Percent_Identity=25.0666666666667, Blast_Score=73, Evalue=9e-13,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 94367; Mature: 94236

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGVGLHVGHPEGYTATD
CCCCCCCCCCCHHCCEEHHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCHHH
ILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKDMQRNWIGKSTGANVTFKVKGTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
KEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQAEAVADYKHQASLKSDLVRTDLAK
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGAYAINPVNGKEMPIWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVE
HHCCCEECCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCHHH
VLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVACLEEKGCGQEKVTYRLRDWLFS
HHCCCCCCHHHCCCCCCEECHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPETELPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
YARFWHKFLYDLGVVPTKEPFQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHIET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCEEEECCHHCCCCCEEEEEC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHH
LDRVYRLITSKEILAENNGALDKAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LYVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIK
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHEEEEEEEC
GKVRAKLMVAKDLSREELQEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK
CCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
SFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGVGLHVGHPEGYTATD
CCCCCCCCCCHHCCEEHHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCHHH
ILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKDMQRNWIGKSTGANVTFKVKGTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
KEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQAEAVADYKHQASLKSDLVRTDLAK
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGAYAINPVNGKEMPIWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVE
HHCCCEECCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCHHH
VLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVACLEEKGCGQEKVTYRLRDWLFS
HHCCCCCCHHHCCCCCCEECHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPETELPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
YARFWHKFLYDLGVVPTKEPFQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHIET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCEEEECCHHCCCCCEEEEEC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHH
LDRVYRLITSKEILAENNGALDKAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LYVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIK
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHEEEEEEEC
GKVRAKLMVAKDLSREELQEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK
CCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA