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The map label for this gene is gldA [H]

Identifier: 116515896

GI number: 116515896

Start: 225727

End: 226815

Strand: Direct

Name: gldA [H]

Synonym: SPD_0237

Alternate gene names: 116515896

Gene position: 225727-226815 (Clockwise)

Preceding gene: 116516937

Following gene: 116517205

Centisome position: 11.03

GC content: 36.55

Gene sequence:

>1089_bases
ATGAGAATTTTTGCTAGTCCTTCTAGATATATTCAGGGGGAAAATGCCTTGTTTGAAAATGCCAAATCAATTTTGGATTT
GGGAAATTACCCTATTCTATTATGCGATCAGTTGGTTTATGATATTGTTGGAAAACGATTTGAAGATTATCTACATAGGT
ATGGTTTCCATATTGTTCTGGCGCTATTTAATGGTGAAGCTTCTGACAATGAAATCAATCGAGTTGTTGCCTTGGCTGAG
AAAGAAAATTGTGATAGTATTATCGGTCTTGGTGGGGGAAAGACGATTGATAGCGCAAAAGCTATTGCAGATTTGATTGA
AAAGCCTGTTATTATTGCTCCAACAATTGCATCGACCGACGCACCTGTATCTGCTTTATCTGTTATTTATACAGATGAAG
GTGCATTTGATCATTATCTATTTTATTCTAAAAATCCAGATTTAGTTTTGGTTGATACAAAAGTTATTTCACAAGCCCCT
AAGCGTTTATTAGCGTCTGGTATTGCAGATGGTTTAGCAACTTGGGTTGAGGCGCGTGCGGTTATGCAGGCAAATGGAAA
AACTATGTTGGGACAACAGCAAACATTGGCTGGAGTTGCAATTGCGAAGAAATGTGAAGAAACGCTGTTTGCAGATGGTT
TACAGGCTATGGCAGCTTGTGAAGCTAAAGTGGTGACACCAGCATTAGAAAATATTGTTGAAGCTAATACTTTATTGAGT
GGTCTAGGTTTTGAAAGTGGAGGATTAGCTGCGGCGCATGCAATTCATAATGGTTTTACTGCATTGACAGGTGACATTCA
TCATTTAACACATGGTGAAAAAGTAGCTTATGGAACTTTAGTACAACTATTATTGGAAAATAGACCTAAAGAAGAACTTG
ATAAGTATATTGAGTTTTACAAAAAAATTGGTATGCCAACAACTCTAAAAGAAATGCATTTGGATCAAGTTGGATATGAT
GATTTAATAAAAGTTGGTAAACAAGCAACTATGGAGGGTGAGACAATTCATCAGATGCCGTTTAAGATTTCGCCTTCAGA
TGTTGCTCAAGCTATTATCGCTGTAGATGCCTATGTAAATTCAAAATAA

Upstream 100 bases:

>100_bases
CTTTCGCCATGCCATCTATCCAAAAGGCGGTTGATGATTTTTCTGACGATTGGTTTGTTACTCAAAATAGTCGTTCCATT
TAGATAGAGAGGAAATACAT

Downstream 100 bases:

>100_bases
ACAATAAGGACTACTGTTTTCCAAATGGTAGTCTTTTATTGATCTCCGTATTGAATTCTATAGAAGATTGAAATAGGATG
AGAACAAATCGATTGGGAAA

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH; GlyDH [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVGKRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAE
KENCDSIIGLGGGKTIDSAKAIADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMAACEAKVVTPALENIVEANTLLS
GLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVAYGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYD
DLIKVGKQATMEGETIHQMPFKISPSDVAQAIIAVDAYVNSK

Sequences:

>Translated_362_residues
MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVGKRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAE
KENCDSIIGLGGGKTIDSAKAIADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMAACEAKVVTPALENIVEANTLLS
GLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVAYGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYD
DLIKVGKQATMEGETIHQMPFKISPSDVAQAIIAVDAYVNSK
>Mature_362_residues
MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVGKRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAE
KENCDSIIGLGGGKTIDSAKAIADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMAACEAKVVTPALENIVEANTLLS
GLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVAYGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYD
DLIKVGKQATMEGETIHQMPFKISPSDVAQAIIAVDAYVNSK

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Is also able to use various diols as substrates, such as propan-1,2-diol, butan-

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=357, Percent_Identity=48.7394957983193, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1786815, Length=301, Percent_Identity=31.8936877076412, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 39198; Mature: 39198

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVGKRFEDYLHRYGFHIVL
CCEECCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
ALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAIADLIEKPVIIAPTIASTD
EEECCCCCCCHHHHHEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCC
APVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAPKRLLASGIADGLATWVEARA
CCHHHEEEEEECCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMAACEAKVVTPALENIVEANTLLS
HHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVAYGTLVQLLLENRPKEELDKYIEFY
HCCCCCCCHHHHHHHHCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
KKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGETIHQMPFKISPSDVAQAIIAVDAYVN
HHCCCCCHHHHHHHHHCCHHHHHHHCHHHCCCCCHHHCCCCEECHHHHHHHHHHHHHHHC
SK
CC
>Mature Secondary Structure
MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVGKRFEDYLHRYGFHIVL
CCEECCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
ALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAIADLIEKPVIIAPTIASTD
EEECCCCCCCHHHHHEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCC
APVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAPKRLLASGIADGLATWVEARA
CCHHHEEEEEECCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMAACEAKVVTPALENIVEANTLLS
HHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVAYGTLVQLLLENRPKEELDKYIEFY
HCCCCCCCHHHHHHHHCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
KKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGETIHQMPFKISPSDVAQAIIAVDAYVN
HHCCCCCHHHHHHHHHCCHHHHHHHCHHHCCCCCHHHCCCCEECHHHHHHHHHHHHHHHC
SK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]